作者机构：
[Liao, Duan-fang; Choudhary, M. Iqbal; Shehla, Nuzhat; Li, Bin; Wang, Wei; Atta-ur-Rahman; Cao, Liang; Jian, Yuqing] Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;[Choudhary, M. Iqbal; Shehla, Nuzhat; Wang, Wei; Atta-ur-Rahman] Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.;[Zhao, Jianping; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, Pharmaceut Sci Res Inst, University, MS 38677 USA.
通讯机构：
[Choudhary, MI ] H;Hunan Univ Chinese Med, TCM & Ethnomed Innovat & Dev Int Lab, Acad Atta Ur Rahman Belt & Rd Tradit Med Res Ctr, Sch Pharm, Changsha, Peoples R China.;Univ Karachi, HEJ Res Inst Chem, Int Ctr Chem & Biol Sci, Karachi, Pakistan.
摘要：
<jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>We focused on the KCNQ1OT1/miR-15a/PD-L1 axis and explored its significance in regulating immune evasion and malignant behaviors of prostate cancer (PC) cells.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>The expression levels of KCNQ1OT1, miR-15a, PD-L1, and CD8 in cells or tissues were examined by RT-qPCR, western blot or immunohistochemistry (IHC) assays. The direct regulations between KCNQ1OT1, miR-15a and PD-L1 were validated by luciferase reporter assay. PC cells were co-cultured with CD8<jats:sup>+</jats:sup>T cells to study the immune evasion. Proliferation, apoptosis, migration and invasion abilities were detected by MTT, flow cytometry, wound healing and Transwell assays, respectively. The cytotoxicity of CD8<jats:sup>+</jats:sup>T cells was determined by LDH cytotoxicity Kit. Epithelial–mesenchymal transition (EMT) and Ras/ERK signaling markers were evaluated by western blot.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>KCNQ1OT1, PD-L1 and CD8 were increased, while miR-15a was decreased in PC tissues. MiR-15a directly bound to the 3′-UTR of PD-L1 and inhibited the expression of PD-L1. Overexpressing miR-15a in PC cells was sufficient to promote cytotoxicity and proliferation, while inhibit apoptosis of CD8<jats:sup>+</jats:sup>T cells, and also suppressed viability, migration, invasion and EMT while promoted apoptosis of PC cells. The above anti-tumor effects of miR-15a were reversed by overexpressing PD-L1. KCNQ1OT1 sponged miR-15a and released its inhibition on PD-L1. Functionally, KCNQ1OT1 in PC cells was essential for suppressing the cytotoxicity of CD8<jats:sup>+</jats:sup>T cells and maintaining multiple malignant phenotypes of PC cells. The Ras/ERK signaling was suppressed after overexpressing miR-15a or knocking down KCNQ1OT1.</jats:p></jats:sec><jats:sec><jats:title>Conclusions</jats:title><jats:p>LncRNA KCNQ1OT1 sponges miR-15a to promote immune evasion and malignant progression of PC via up-regulating PD-L1.</jats:p></jats:sec>
期刊：
Journal of Pharmaceutical and Biomedical Analysis,2020年177:112875 ISSN：0731-7085
通讯作者：
Liu, RX;Wang, W
作者机构：
[Liu, Wanhui; Liu, RX; Liu, Rongxia; Liu, Qianqian] Yantai Univ, Key Lab Mol Pharmacol & Drug Evaluat, Sch Pharm, Minist Educ,Collaborat Innovat Ctr Adv Drug Deliv, Yantai 264005, Peoples R China.;[Cai, Xiong; Li, Bin; Wang, Wei; Liu, Leping] Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Innovat Mat Med Res Inst, Changsha 410208, Hunan, Peoples R China.;[Cheng, Dongsheng] Yantai Yuhuangding Hosp, Yantai 264000, Peoples R China.
通讯机构：
[Liu, RX ] Y;[Wang, W ] H;Yantai Univ, Key Lab Mol Pharmacol & Drug Evaluat, Sch Pharm, Minist Educ,Collaborat Innovat Ctr Adv Drug Deliv, Yantai 264005, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, TCM & Ethnomed Innovat & Dev Int Lab, Innovat Mat Med Res Inst, Changsha 410208, Hunan, Peoples R China.
关键词：
carboxyl group;lactone derivative;methanol;schisanlactone e;unclassified drug;herbaceous agent;schisanlactone E;triterpene;accuracy;animal experiment;area under the curve;Article;dehydrogenation;drug bioavailability;drug excretion;drug metabolism;half life time;hydrophilicity;hydroxylation;in vivo study;isomer;Kadsura;Kadsura heteroclita;male;matrix effect;maximum plasma concentration;mean residence time;multiple reaction monitoring;nonhuman;physical chemistry;plasma concentration-time curve;precipitation;priority journal;qualitative analysis;quantitative analysis;rat;solid phase extraction;sulfation;supernatant;tandem mass spectrometry;time to maximum plasma concentration;ultra performance liquid chromatography;volume of distribution;animal;animal model;bioavailability;chemistry;feces;high performance liquid chromatography;intestinal excretion;Kadsura;oral drug administration;plant stem;procedures;tandem mass spectrometry;urinary excretion;Administration, Oral;Animals;Biological Availability;Chromatography, High Pressure Liquid;Drugs, Chinese Herbal;Feces;Intestinal Elimination;Kadsura;Male;Models, Animal;Plant Stems;Rats;Renal Elimination;Solid Phase Extraction;Tandem Mass Spectrometry;Triterpenes
作者：
Li, Jiang;Hu, Jia-yuan;Zhai, Jing-bo;Niu, Jun-qiang;Kwong, Joey S. W.;...
期刊：
Complementary Therapies in Medicine,2019年46:180-188 ISSN：0965-2299
通讯作者：
Dai, M;Tian, Gui-hua;Shan, HC
作者机构：
[Dai, Min; Li, Jiang; Dai, M] Chinese Acad Med Sci, Natl Canc Ctr, Canc Hosp, 17 Panjiayuannanli, Beijing, Peoples R China.;[Dai, Min; Li, Jiang; Dai, M] Peking Union Med Coll, 17 Panjiayuannanli, Beijing, Peoples R China.;[Tian, Gui-hua; Hu, Jia-yuan; Shan, Hong-cai] Beijing Univ Chinese Med, Dongzhimen Hosp, 5 Haiyuncang Alley, Beijing, Peoples R China.;[Zhai, Jing-bo] Tianjin Univ Tradit Chinese Med, Tianjin, Peoples R China.;[Niu, Jun-qiang] Lanzhou Univ, Hosp 1, Lanzhou, Gansu, Peoples R China.
通讯机构：
[Shan, HC ; Tian, GH] B;[Dai, M ] C;Chinese Acad Med Sci, Natl Canc Ctr, Canc Hosp, 17 Panjiayuannanli, Beijing, Peoples R China.;Peking Union Med Coll, 17 Panjiayuannanli, Beijing, Peoples R China.;Beijing Univ Chinese Med, Dongzhimen Hosp, 5 Haiyuncang Alley, Beijing, Peoples R China.
关键词：
N-of-1 trial;Traditional Chinese medicine;Reporting guidance;CONSORT
作者机构：
[Liu, Yongbei; Dang, Wenya; Liu, Bin; Zhou, Hongyan; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;[Yang, Yupei; Qin, Yan; Wang, Wei; Liu, Bin; Li, Bin; Liu, Yongbei] Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.;[Zou, Wei] Hunan Prov Maternal & Child Hlth Care Hosp, NHC Key Lab Birth Defects Res Prevent & Treatment, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Liu, B ; Wang, W ; Liu, B] H;Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Pharm, Innovat Mat Med Res Inst, TCM & Ethnomed Innovat & Dev Int Lab, Changsha 410208, Hunan, Peoples R China.
摘要：
T4 polynucleotide kinase (PNK) is the primary member of the 5'-kinase family that can transfer the gamma-phosphate residue of ATP to the 5'-hydroxyl group of oligonucleotides. In this article, using the differential quenching ability of reduced graphene oxide (rGO) towards the fluorophore-labeled DNA probe, we propose a novel method for detecting T4 PNK activity assisted by ligase reaction. Under the optimized conditions, the detection limit of T4 PNK was estimated to be 0.0002 U muL(-1) in the linear region of 0.001 U muL(-1)-0.1 U muL(-1). Additionally, the developed method was used to screen regulators of T4 PNK from natural compounds. The compound f isolated from the root of Kadsura coccinea (Lem.) A.C. Smith was found to stimulate T4 PNK activity in a concentration-dependent manner in vitro. Finally, the method was used to monitor the relation of T4 PNK activity with pelvic inflammatory disease (PID). The results demonstrated that the development of this disease could inhibit T4 PNK activity to some extent. In summary, the above data indicate that the method not only provides a universal platform for monitoring T4 PNK activity, but also shows great potential to be used in drug screening and clinic diagnosis.
摘要：
Objective To observe the effects and possible mechanism of Ziyin Mingmu Pills (滋阴明目丸) on retinal pigment epithelial (RPE) cells after light injury. Methods A total of 60 SD rats were divided into Ziyin Mingmu Pills low,medium and high dose groups and a blank group,with 15 rats in each group. The groups of different Ziyin Mingmu Pills dosages were respectively administered with the suspension of Ziyin Mingmu Pills at a concentration of 0.39,0.78,and 1.56 g /ml. The gavage volume was 34 ml /(kg·d),and the blank group was given 34 ml /(kg·d) of normal saline for gavage,each group was administered for 7 days. The drug-containing serum was prepared in the end of the gavage,and the concentration of the drug-containing serum in the subsequent experiment was screened by the MTT method. The selected cells for experiment were divided into a blank group (without drugcontaining serum),serum group (with drug-containing serum),model group (light damage modeling,no drug-containing serum),and Chinese medicine group (light damage modeling with drug-containing serum). After 24 hours of culture,the in vitro RPE cell light damage models of model group and the Chinese medicine group were established. The apoptosis rate of each group was measured,and the expression of caspase-1,caspase-3 and mRNA were measured. Results The medium dose serum was the optimal dose,and it was used in subsequent experiments. Compared with the blank group,the apoptosis rate,expression of Caspase-1 and Caspase-3 protein and mRNA in serum group were not statistically significant (P > 0.05). The apoptosis rate,Caspase-1 and Caspase-3 protein and mRNA expression in the model group increased significantly (P < 0.05). Compared with the model group,the apoptosis rate,Caspase-1 and Caspase-3 protein and mRNA expression in the Chinese medicine group were significantly decreased (P < 0.05). Conclusion Ziyin Mingmu Pills can effectively inhibit the apoptosis of RPE cells after light injury. The mechanism may be related to the inhibition of the expression of Caspase-1,Caspase-3 protein and its mRNA.
摘要：
Acute pancreatitis (AP) is defined as an acute inflammation of pancreas that may cause damage to other tissues and organs depending upon the severity of symptoms. The diagnosis of AP is usually made by detection of raised circulating pancreatic enzyme levels, but there are occasional false positive and false negative diagnoses and such tests are often normal in delayed presentations. More accurate biomarkers would help in such situations. In this study, the global metabolites' changes of AP patients (APP) were profiled by using gas chromatography-mass spectrometry (GC-MS). Multivariate pattern recognition techniques were used to establish the classification models to distinguish APP from healthy participants (HP). Some significant metabolites including 3-hydroxybutyric acid, phosphoric acid, glycerol, citric acid, D-galactose, D-mannose, D-glucose, hexadecanoic acid and serotonin were selected as potential biomarkers for helping clinical diagnosis of Ap. Furthermore, the metabolite changes in APP with severe and mild symptoms were also analyzed. Based on the selected biomarkers, some relevant pathways were also identified. Our results suggested that GC-MS based serum metabolomics method can be used in the clinical diagnosis of AP by profiling potential biomarkers. (C) 2017 LAP and EPC. Published by Elsevier B.V. All rights reserved.
作者机构：
[常小荣; 刘迈兰; 周芝根; 刘灿; 杨茜芸] College of Acupuncture-Moxibustion and Massage, Hunan University of CM, Changsha 410208, China;[李波] the First Affiliated Hospital of Hunan University of CM
作者机构：
[刘灿; 周芝根; 杨茜芸; 常小荣; 刘迈兰] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208, China;[李波] The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[周杰; 陈勤] The Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, 310053, China
通讯机构：
[Mai-lan Liu] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Point, Zusanli (ST 36);Central Mechanism;Functional Magnetic Resonance Imaging;Specificity of Acupoints;Research on Acupoints
摘要：
The contents about the central action mechanisms of needling Zusanli (ST 36) were sorted by summarizing the relevant literatures published in the past 10 years, and it was found that: by comparing acupuncture at Zusanli (ST 36), other acupoints or sham points, most studies showed that Zusanli (ST 36) had relatively specific characteristics in the brain function response; studies on the central action mechanisms of Zusanli (ST 36) were mainly focused on sequelae of cerebral apoplexy, pain, gastrointestinal diseases, weight loss and drug addiction withdrawal and so on; acupuncture manipulations, stimulation methods, individual differences, stimulation quantity, and stimulation duration, etc. could also induce different brain function responses; acupuncture at Zusanli (ST 36) had an after-working effect, also called long-term effect, but with poor repeatability. So, it was suggested that the future studies should focus on the factors that affect the clinical efficacies and experimental results, including the compatibility effects of the acupoints, reproducibility of research results, sample size, acupuncture methods, long-term effects and disease entities.
摘要：
Two 9,10-dihydrophenanthrenes trivially named phocantol and phocantone, two diterpenoid glycosidesnamed phocantoside A and phocantoside B were isolated from the ethanol extract of the air-dried whole plant of Pholidota cantonensis Rolfe, together with seventeen known compounds. The structures of the four compounds were identified as 1-hydroxy-2,7-dimethoxy-9,10-dihydrophenanthro-[4,5-bcd]furan, 5-hydroxy-2,7-dimethoxy-9,10-dihydro-1,4-phenanthrenedione, (8R,13E)-ent-labd-13-ene-3α,8,15-triol 15-O-β-D-gluco-pyranoside and (5S,8R,9S,10R)-cis-cleroda-3,13(E)-diene-15,18-diol 15-O-β-D-glucopyranosyl-18-O-β-D-glucopyranoside by chemical and spectroscopic methods, including 1D and 2D NMR. Twenty compounds were evaluated for their cytotoxic activities against mouse leukemia p388D1 cancer cells, and compound phocantone, phocantoside A, tanshinone IIA and syringate exhibited cytotoxic activity against the mouse leukemia p388D1 cancer cells with IC50 values ranging from 13.37 to 27.5 μM.
作者机构：
[刘迈兰; 刘灿; 周芝根; 郁洁; 常小荣] School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208, China;[吴静; 戴培山] School of Information Science and Engineering, Central South University, Changsha, 410002, China;[李波] Radiology Department, the First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China
通讯机构：
[Jie Yu; Xiao-rong Chang] S;School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
