作者机构：
[Jing Tan; Lu Sun; Yan-ping Chen; Ren-da Yang; Xiao-rong Chang] Hunan University of Chinese Medicine, Changsha, China;Xiniujiao Community Health Service Station, Guangdong, China;[Chong-zheng Zhu] Huizhou Hospital of Traditional Chinese Medicine, Guangdong, China;[Feng-jiao Luo] Department of Stomach Surgery, Sun Yat-sen University Cancer Center, Guangdong, China;[Wen-tao Huang] The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China
通讯机构：
[Xiao-rong Chang] H;Hunan University of Chinese Medicine, Changsha, China
关键词：
Moxibustion Therapy;Herbal Cake-partitioned Moxibustion;Hyperlipidemias;Laurocapram;Isoborneol;Sterol Esterase;Hydroxymethylglutaryl CoA Reductases;Rabbits
摘要：
Objective: To observe the effects of laurocapram and borneol as transdermal penetration enhancers applied to herbal cake-partitioned moxibustion on liver lipids, hormone-sensitive lipase (HSL) and hydroxymethylglutaryl CoA (HMG-CoA) reductase in hyperlipidemia rabbits. Methods: Forty New-Zealand rabbits were randomly divided into 5 groups using the random number table method, with 8 rats in each group. Rabbits in the blank group were fed routinely with a normal diet; rabbits in the other groups were fed with high-fat diet for 12 weeks to establish the hyperlipidemia model. Rabbits in the blank and the model groups were not given any intervention. After the model was prepared successfully, rabbits in the non-transdermal penetration enhancer group received herbal cake-partitioned moxibustion without transdermal penetration enhancers; rabbits in the laurocapram group and the borneol group received herbal cake-partitioned moxibustion with laurocapram or borneol respectively. After 4 weeks of treatment, the serum was isolated and enzyme-linked immunosorbent assay (ELISA) was applied for the detection of HSL and HMG-CoA reductase. The liver tissues were isolated, and total cholesterol (TC) and triglycerides (TG) were measured by enzymatic methods. One-step method was applied for high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) detection, and transmission turbidimetry was for apolipoprotein A1 (Apo-A1) and apolipoprotein B (Apo-B) detection. Results: The serum concentrations of the drugs in the laurocapram and the borneol groups were significantly higher than those in the non-transdermal penetration enhancer group (both P<0.05); all drug penetrations in the borneol group were significantly higher than those in the laurocapram group (both P<0.05), except for tanshinone ⅡA. Compared with the non-transdermal penetration enhancer group, the HSL was significantly increased while the HMG-CoA reductase was significantly decreased in the laurocapram and the borneol groups (both P<0.05); between groups, the HSL in the borneol group was significantly higher than that in the laurocapram group (P<0.05). Compared with the blank group, the levels of LDL-C, TG, TC and Apo-B in rabbit liver were significantly increased in the model group (P<0.05); compared with the model group, the levels of LDL-C, TG, TC and Apo-B in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups were significantly decreased (all P<0.05); between groups, the TG and TC in the laurocapram group and the LDL-C, TG, TC and Apo-B in the borneol group were significantly lower than those in the non-transdermal penetration enhancer group (all P<0.05), and the TG, LDL-C and Apo-B in the borneol group were significantly lower than those in the laurocapram group (all P<0.05). Compared with the blank group, the HDL-C and Apo-A1 were significantly decreased in the model group (both P<0.05), while compared with the model group, the HDL-C and Apo-A1 were significantly increased in the non-transdermal penetration enhancer, the laurocapram, and the borneol groups (all P<0.05). Between groups, the Apo-A1 in the laurocapram group, the HDL-C and Apo-A1 in the borneol group were significantly higher than those in the non-transdermal penetration enhancer group (all P<0.05). Conclusion: The application of laurocapram and borneol, as transdermal penetration enhancers, in herbal cake-partitioned moxibustion can promote the penetration of the drugs in the herbal cake, increase the levels of HDL-C and Apo-A1, improve the metabolism of HSL and HMG-CoA reductase, and also simultaneously reduce the levels of TC, TG, LDL-C and Apo-B in the liver. The transdermal penetration enhancement effect of borneol is slightly better than or equivalent to that of laurocapram.
作者机构：
[刘兵] Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China;[王华] Hubei University of CM;[周仲瑜] Department of Acupuncture and Moxibustion, Hubei Provincial Hospital of TCM;[常小荣] Acupuncture-Moxibustion and Tuina School of Hunan University of CM;[章薇] First Affiliated Hospital of Hunan University of CM
摘要：
中风(stro k e)是一种严重危害人类健康和生命安全的常见的难治性疾病,为了系统探讨中风的分子生物学研究进展,总结近年来分子生物学领域治疗中风相关的临床实验和文献资料,从分子生物学、分子免疫学等方面研究中风的进展,在药物使用、分子机理、临床疗效等方面进行整理、分析及总结,展望分子生物学在中风疾病的应用以及未来研究前景.
作者机构：
[Zhang, Weibo; Wang, Guangjun; Jia, Shuyong; Li, Hongyan; Wang, GJ; Zhang, WB; Song, Xiaojing] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.;[Chang, Xiaorong; Liu, Mi] Hunan Univ Chinese Med, Acupuncture & Tuina Sch, Changsha, Peoples R China.
通讯机构：
[Wang, GJ; Zhang, WB] C;[Chang, Xiaorong] H;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.;Hunan Univ Chinese Med, Acupuncture & Tuina Sch, Changsha, Peoples R China.
摘要：
The physiologically important relationship between oxygen saturation and blood flow is not entirely understood, particularly with regard to the multiple velocity components of flow and temperature. While our previous studies used classic laser Doppler flowmetry combined with an enhanced perfusion probe to assess local blood flow following thermal stimulation, oxygen saturation signals were not assessed. Thus, the current study used multiscale entropy (MSE) and multiscale fuzzy entropy (MFE) to measure the complexity of oxygen saturation signals following thermal stimulation in healthy subjects. The results indicate that thermal stimulation increases oxygen saturation and affects the measured signal complexity in a temperature-dependent fashion. Furthermore, stimulus temperature not only affects the correlation between speed-resolved blood perfusion and oxygen saturation, but also the correlation between the complexity area indices (CAI) of the two signals. These results reflect the complexity of local regulation and adaptation processes in response to stimuli at different temperatures.
