作者： Wang, Dong-Xin;Yang, Ying;Huang, Xiao-Song;Tang, Jia-Yu;Zhang, Xi;...

期刊： Neurochemistry International,2021年146(1):104972 ISSN：0197-0186

通讯作者： Li, XS;Wang, XY

作者机构： [Wang, Dong-Xin; Li, Xiao-Song] Hunan Prov Brain Hosp, Res Inst Mental & Sanitat, 427,Sect 3 Furong Middle Rd, Changsha 410007, Hunan, Peoples R China.;[Yang, Ying; Huang, Xiao-Song; Li, Xiao-Hui; Tang, Jia-Yu] Hunan Prov Brain Hosp, Med Dept Neurol, Changsha 410007, Hunan, Peoples R China.;[Zhang, Xi] Hunan Tradit Chinese Med Univ, Clin Med Sch, Changsha 410006, Hunan, Peoples R China.;[Zhou, Bin; Huang, Hong-Xing; Liu, Bo; Liu, Kun; Zou, Shu-Cheng] Hunan Prov Brain Hosp, Dept Neurosurg, Changsha 410007, Hunan, Peoples R China.;[Xiao, Hui-Qiong] Hunan Prov Brain Hosp, Dept Sci Res, Changsha 410007, Hunan, Peoples R China.

通讯机构： [Li, XS ; Wang, XY ] H;Hunan Prov Brain Hosp, Res Inst Mental & Sanitat, 427,Sect 3 Furong Middle Rd, Changsha 410007, Hunan, Peoples R China.;Hunan Prov Brain Hosp, Inst Clin Trials Drugs, 427,Sect 3 Furong Middle Rd, Changsha 410007, Hunan, Peoples R China.

关键词： 1 methyl 4 phenylpyridinium;1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine;adenovirus E1B protein;lactate dehydrogenase;microRNA;microRNA 96;parkin;pramipexole;protein bcl 2;protein BNip3;unclassified drug;antiparkinson agent;BNip3 protein, mouse;membrane protein;microRNA;Mirn96 microRNA, mouse;mitochondrial protein;pramipexole;animal experiment;animal model;animal tissue;apoptosis;Article;behavior assessment;cell viability;controlled study;enzyme release;gene overexpression;gene targeting;human;human cell;immunofluorescence;male;mitophagy;mouse;MPP+-induced neurotoxicity;nerve injury;neuroprotection;nonhuman;Parkinson disease;priority journal;protein targeting;real time reverse transcription polymerase chain reaction;TUNEL assay;Western blotting;animal;C57BL mouse;dose response;drug effect;metabolism;mitophagy;nerve cell;parkinsonism;physiology;tumor cell line;Animals;Antiparkinson Agents;Cell Line, Tumor;Dose-Response Relationship, Drug;Humans;Male;Membrane Proteins;Mice;Mice, Inbred C57BL;MicroRNAs;Mitochondrial Proteins;Mitophagy;Neurons;Parkinsonian Disorders;Pramipexole

摘要： Background: Parkinson's disease is a common neurodegenerative problem. Pramipexole (PPX) plays protective role in Parkinson's disease. Nevertheless, the mechanism of PPX in Parkinson's disease-like neuronal injury is largely uncertain. Methods: 1-methyl-4-phenylpyridinium (MPP+)-stimulated neuronal cells and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice were used as the model of Parkinson's disease. MPP+-induced neuronal injury was assessed via cell viability, lactic dehydrogenase (LDH) release and apoptosis. microRNA-96 (miR-96) and BCL2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) abundances were examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) or Western blotting. Mitophagy was tested by Western blotting and immunofluorescence staining. MPTP-induced neuronal injury in mice was investigated via behavioral tests and TUNEL. Results: PPX alleviated MPP+-induced neuronal injury via increasing cell viability and decreasing LDH release and apoptosis. PPX reversed MPP+-induced miR-96 expression and inhibition of mitophagy. miR-96 overexpression or BNIP3 interference weakened the suppressive role of PPX in MPP+-induced neuronal injury. miR-96 targeted BNIP3 to inhibit PTEN-induced putative kinase 1 (PINK1)/Parkin signals-mediated mitophagy. miR-96 overexpression promoted MPP+-induced neuronal injury via decreasing BNIP3. PPX weakened MPTP-induced neuronal injury in mice via regulating miR-96/BNIP3-mediated mitophagy. Conclusion: PPX mitigated neuronal injury in MPP+-induced cells and MPTP-induced mice by activating BNIP3-mediated mitophagy via directly decreasing miR-96. © 2021 Elsevier Ltd

语种： 英文

作者： 彭琼;杨萍;黄红星;卢军;王琴;...

期刊： 中国医师杂志,2020年22(7):1005-1008 ISSN：1008-1372

作者机构： [彭琼; 杨萍; 黄红星; 卢军; 王琴; 黄亚辉; 匡卫平] Department of Neurosurgery, College of Clinical Medicine, Hunan University of Chinese Medicine, Changsha, 410007, China

关键词： 迷走神经刺激术;癫痫;抑郁;细胞因子类;神经递质

摘要： 目的:探讨迷走神经刺激(VNS)对癫痫合并抑郁患者血清细胞因子及神经递质的影响。方法:选取2015年3月至2018年3月湖南省脑科医院收治的13例癫痫合并抑郁并行VNS手术患者，比较其术前、术后6个月及12个月时手术疗效、抑郁程度、血清细胞因子及神经递质改变情况。结果:VNS术后6、12个月时患者癫痫治疗有效率分别为61.5%(8/13)、76.9%(10/13)。术后6个月及12个月，患者汉密顿抑郁量表(HAMD)、抑郁自评量表(SDS)分值均较术前明显降低( P<0.05或 P<0.01)。术后6个月时血清5-羟色胺(5-HT)水平较术前明显升高，IL-1α、IL-6、TNF-α较术前明显降低，差异有统计学意义( P<0.05或 P<0.01)；术后12个月时血清5-HT、多巴胺(DA)较术前明显升高，IL-1α、IL-2、IL-6、TNF-α较术前明显降低，差异有统计学意义( P<0.05或 P<0.01)。 结论:VNS可能通过调节神经递质及血清细胞因子水平，使癫痫及抑郁均得到不同程度改善。

语种： 中文

作者： 周彬;黄红星;杨萍;匡卫平;卢军;...

期刊： 中国医师杂志,2019年21(11):1617-1620 ISSN：1008-1372

作者机构： 湖南省脑科医院神经外科,湖南中医药大学临床医学院,长沙410007;[黄红星; 朱勇; 卢军; 匡卫平; 杨萍; 王琴; 周彬] 湖南省脑科医院

关键词： 癫痫,颞叶;柴胡龙骨牡蛎汤;脑电描记术;神经生长因子;脑源性神经营养因子

摘要： 目的探讨柴胡加龙骨牡蛎汤（CLMD）对颞叶癫痫患者脑电图及血清神经生长因子（NGF）、脑源性神经营养因子（BDNF）水平的影响。方法将138例颞叶癫痫患者,采用单盲随机对照实验设计,分为奥卡西平组67例、奥卡西平+CLMD组71例,治疗28 d后,观察两组脑电图变化、血清中NGF、BDNF变化等。结果奥卡西平+CLMD组脑电图有效率为81. 8%,明显高于奥卡西平组的50. 7%,差异有统计学意义（P <0. 01）;两组治疗后血清NGF、BDNF水平均高于治疗前（P <0. 05）,且奥卡西平+CLMD组血清NGF、BDNF水平高于奥卡西平组（P <0. 05）;两组药物不良反应相关血清学指标比较差异无统计学意义（P> 0. 05）。结论 CLMD联合奥卡西平疗效优于单用奥卡西平,可明显提高颞叶癫痫患者NGF、BDNF水平,减少颞叶癫痫患者痫样放电,且安全性高。

语种： 中文

作者： Yang, Ping;Li, Liang;Zhang, Wei;Liu, Bo;Li, Ling;...

期刊： FRONTIERS IN NEUROLOGY,2019年10(APR):433143 ISSN：1664-2295

通讯作者： Zou, SC;Li, F

作者机构： [Liu, Hua; Li, Ling; Yang, Ping; Liu, Bo; Liu, Kun; Zou, Shucheng; Huang, Hongxing] Hunan Univ Chinese Med, Hunan Brain Hosp, Dept Neurosurg, Clin Med Sch, Changsha, Hunan, Peoples R China.;[Li, F; Li, Feng; Li, Liang; Huang, Huiyong] Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.;[Zhang, Wei] Hunan Univ Chinese Med, Discipline Chinese & Western Integrat Med, Changsha, Hunan, Peoples R China.;[Li, F; Li, Feng] Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90024 USA.

通讯机构： [Li, F ; Zou, SC ] H;Hunan Univ Chinese Med, Hunan Brain Hosp, Dept Neurosurg, Clin Med Sch, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.;Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90024 USA.

关键词： hemangioblastomas;RNF139;Mutation;next-generation sequencing (NGS);Sanger sequencing

摘要： Hemangioblastomas (HBs) are classified as grade I tumors with uncertain origin according to the World Health Organization's classification system. HBs are characterized by rich mesenchymal cells and abundant capillaries. It has been shown that tumorigenesis of HBs depends on mutational inactivation of Von Hippel-Lindau (VHL) tumor suppressor gene. Therefore, the majority of patients will undergo VHL single gene test, and sequencing scheme is rarely used in clinic. In this study, we described a girl and her father successively found to have HBs within half a year. The results of next-generation sequencing (NGS) and Sanger sequencing analysis showed that both of them carried heterozygous mutation of RNF139 p.Q650R. This mutation was interpreted as Pathogenic variation based on the American College of Medical Genetics and Genomics (ACMG) guideline. Sanger sequencing was performed with other family members. No mutation on rs118184842 locus of RNF139 gene was found in the samples from the girl's mother, uncle and aunt. This report supports that the novel mutation of RNF139 p.Q650R probably serve as a key role in HBs progression.

语种： 英文

作者： 刘坤;黄红星;罗琳（罗琳）

期刊： 中国实用神经疾病杂志,2017年20(21):60-63 ISSN：1673-5110

作者机构： 湖南省第二人民医院(湖南省脑科医院)神经外科,湖南长沙,410007;湖南中医药大学医学院,湖南长沙,410208;[罗琳] 湖南中医药大学;[刘坤; 黄红星] 湖南省脑科医院

关键词： 大脑中动脉瘤;显微手术;脑血管痉挛;电针;尼莫地平;破裂;蛛网膜下腔出血;脑梗死

摘要： 目的 探讨电针配合尼莫地平治疗大脑中动脉瘤夹闭术后脑血管痉挛的疗效.方法 回顾性分析60例经显微夹闭术处理破裂大脑中动脉瘤患者,术后T CD证实不同程度脑血管痉挛,30例术后应用尼莫地平持续泵入(甲组),连用14 d;30例在尼莫地平组治疗基础上加用电针疗法(乙组),选用下关、百会及风池穴.术后3 d、7 d及14 d评估GCS评分、脑血管痉挛程度、mRS评分等.结果 术后7 d和14 d,乙组GCS评分明显高于甲组,脑血管痉挛为轻度比例达76.7%、93.3%;术后1个月随访,乙组mRS评分好于甲组,差异均有统计学意义(P<0.05);而术后3 d,2组GCS评分及脑血管痉挛恢复程度差异无统计学意义(P>0.05).结论 尼莫地平配合电针对大脑中动脉瘤破裂所致脑血管痉挛有一定改善作用,时间越长,效果可能越明显.

语种： 中文

作者： 周彬;黄红星;匡卫平;蔡溢

期刊： 立体定向和功能性神经外科杂志,2013年26(1):20-23 ISSN：1008-2425

作者机构： 410007长沙,湖南省脑科医院神经外科、湖南中医药大学临床学院;[周彬; 黄红星; 匡卫平; 蔡溢] 湖南脑科医院

关键词： 复发性躁狂症;立体定向手术;疗效;安全性

摘要： 目的 探讨立体定向靶点毁损术治疗复发性躁狂症的疗效及安全性.方法 对40例复发性躁狂症患者行立体定向杏仁核、扣带回、伏隔核靶点毁损术,分别在术前2天与术后2周、6个月、1年由精神科医师进行临床疗效总评量表(clinical global impression,CGI)、躁狂量表(bech-rafaelsen mania rating scale,BRMS)、阳性和阴性症状量表(positive and negative symptom scale,PANSS)及精神症状简明量表(brief psychiatric rating scale,BPRS)对疗效进行评定,采用日常能力量表(activies of daily living,ADL)、社会功能缺陷量表(social disability screening schedule,SDSS)评估手术安全性.结果 术后2周、6个月、1年与术前相比,临床疗效总评量表(CGI)、躁狂量表(BRMS)、阳性和阴性症状量表(PANSS)、精神症状简明量表(BPRS)、日常能力量表(ADL)及社会功能缺陷量表(SDSS)评分有明显差异(P＜0.05或P＜0.01),且无严重并发症和后遗症.结论 立体定向杏仁核、扣带回、伏隔核靶点毁损术可以有效缓解患者躁狂状态,提高药物敏感性,明显改善患者生存质量.

语种： 中文