作者机构：
[李玲] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[杨婷婷; 邱婷; 王健; 朱梦晨] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[李点] Health Management Section, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China
作者机构：
[刘洋; 张仪; 胡旭东] School of Basic Chinese Medical Science, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China;[童巧珍] Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China;[陈聪; 廖菁] School of Basic Chinese Medical Science, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China<&wdkj&>Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China
通讯机构：
[LIAO Jing] S;School of Basic Chinese Medical Science, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China<&wdkj&>Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 401208, China
作者机构：
The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China;[杨建文; 林亚平; 彭艳; 张程程; 陈海交; 魏星] The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha 410000, China;[曹建中] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410000, China
通讯机构：
[Zhang Cheng-cheng; Peng Yan] T;The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China<&wdkj&>The Domestic First-class Discipline Construction Project of Chinese Medicine of Hunan University of Chinese Medicine, College of Acupuncture & Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Calcium-activated Chloride Channel;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要：
To investigate the mechanisms of electroacupuncture (EA) at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) in intervening diabetic gastroparesis (DGP) based on calcium-activated chloride channel. Forty Sprague-Dawley rats were randomly divided into four groups, including a normal control group (group A), a model group (group B), an EA group (group C) and a metoclopramide group (group D), with 10 rats in each group. A single intraperitoneal injection of 2% streptozotocin (STZ) combined with 8-week high-glucose high-fat diet was used to establish a DGP rat model. After intervention, gastrointestinal propulsive rate was observed; the expression level of transmembrane protein 16A (TMEM16A) was examined by immunohistochemistry; the Ca2+ concentration in interstitial cells of Cajal (ICCs) was detected by immunofluorescence; and whole-cell patch-clamp technique was applied to detect the current intensity of calcium-activated chloride channel (ICaCC) in ICCs in gastric antrum. After modeling, the blood glucose levels in group B, group C and group D were significantly increased compared with group A (all P<0.01); after intervention, compared with group B, the blood glucose levels in group C and group D were significantly decreased (P<0.05, P<0.01); the intra-group comparison of blood glucose level between after modeling and after intervention found significant difference only in group C (P<0.01). The gastrointestinal propulsive rates in group B, group C and group D were significantly different from that in group A (P<0.01 or P<0.05); the gastrointestinal propulsive rates were markedly higher in group C and group D than in group B (P<0.01, P<0.01). The expressions of TMEM16A in group B and group C were decreased compared with group A (P<0.01, P<0.05); the expressions of TMEM16A in group C and group D were increased compared with group B (P<0.01, P<0.05). The fluorescence intensity of Ca2+ was significantly lower in group B than in group A (P<0.01); the fluorescence intensity of Ca2+ was significantly higher in group C and group D than in group B (P<0.01, P<0.05). ICaCC in ICCs in group B was significantly decreased compared with group A; ICaCC in group C and group D were increased compared with group B. EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) can significantly improve gastrointestinal motility in DGP rats by up-regulating the ICaCC in ICCs.
摘要：
Recent research evidence documents that lncRNAs (long non-coding RNAs lncRNAs) play a pivotal role in the tumorigenesis and development of tumors. LncRNA SNGH3 (small nucleolar RNA host gene 3) is highly expressed in numerous forms of cancer, serving as an oncogene in cancer progression. Nonetheless, the clinical relationship, along with the mechanism of SNGH3 in bladder cancer, have not been studied. Herein, the findings exhibited upregulation of SNGH3 in bladder cancer tissues, along with the cell lines. Furthermore, overexpressed SNGH3 was positively linked to the TNM stage, as well as the histological grade of bladder cancer. Moreover, the silencing of SNGH3, using CRISPR-dCas9, suppressed cell growth along with migration, but elevated bladder cancer cell apoptosis. In summary, we established that SNGH3 serves as a bladder cancer oncogene and could be employed as a prospective diagnostic marker for clinical use, and is also a therapeutic target for CRISPR-mediated gene therapy.
期刊：
Evidence-Based Complementary and Alternative Medicine,2021年2021 ISSN：1741-427X
作者机构：
[Wang, Yaxin; Zhou, Xiaowen; Li, Xiantao; Yan, Zhenqian; Ren, Qi] Guangzhou Univ Chinese Med, Sch Basic Med Sci, Lab TCM Syndrome Essence & Objectificat, Guangzhou 510006, Peoples R China.;[Liu, Xiaoqi] Guangzhou Sagene Tech Co Ltd, Guangzhou 510006, Peoples R China.;[Fang, Ge] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.;[Wang, Bin] Shenzhen Tradit Chinese Med Hosp, Shenzhen 518000, Peoples R China.
摘要：
Background. Hyperlipidemia, due to the practice of unhealthy lifestyles of modern people, has been a disturbance to a large portion of population worldwide. Recently, several scholars have turned their attention to Chinese medicine (CM) to seek out a lipid-lowering approach with high efficiency and low toxicity. This study aimed to explore the mechanism of Huatan Jiangzhuo decoction (HTJZD, a prescription of CM) in the treatment of hyperlipidemia and to determine the major regulation pathways and potential key targets involved in the treatment process. Methods. Data on the compounds of HTJZD, compound-related targets (C-T), and known disease-related targets (D-T) were collected from databases. The intersection targets (I-T) between C-T and D-T were filtered again to acquire the selected targets (S-T) according to the specific index. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, as well as network construction, were applied to predict the putative mechanisms of HTJZD in treating hyperlipidemia. Thereafter, an animal experiment was conducted to validate the therapeutic effect of HTJZD. In addition, regulated differentially expressed genes (DEGs) were processed from the RNA sequencing analysis results. Common genes found between regulated DEGs and S-T were analyzed by KEGG pathway enrichment to select the key targets. Lastly, key targets were validated by real-time quantitative reverse transcription PCR (qRT-PCR) analysis. Results. A total of 210 S-T were filtered out for enrichment analysis and network construction. The enrichment results showed that HTJZD may exert an effect on hyperlipidemia through the regulation of lipid metabolism and insulin resistance. The networks predict that the therapeutic effect of HTJZD may be based on the composite pharmacological action of these active compounds. The animal experiment results verify that HTJZD can inhibit dyslipidemia in rats with hyperlipidemia, suppress lipid accumulation in the liver, and reverse the expression of 202 DEGs, which presented an opposite trend in the model and HTJZD groups. Six targets were selected from the common targets between 210 S-T and 202 regulated DEGs, and the qRT-PCR results showed that HTJZD could effectively reverse Srebp-1c, Cyp3a9, and Insr mRNA expression (P<0.01). Conclusion. In brief, network pharmacology predicted that HTJZD exerts a therapeutic effect on hyperlipidemia. The animal experimental results confirmed that HTJZD suppressed the pathological process induced by hyperlipidemia by regulating the expression of targets involved in lipid metabolism and insulin resistance.
期刊：
OncoTargets and Therapy,2021年14:1239-1248 ISSN：1178-6930
通讯作者：
Shen, Li
作者机构：
[Li, Jinxia] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Peoples R China.;[Wang, Yiping] Zhejiang Chinese Med Univ, Key Lab Digest Pathophysiol Zhejiang Prov, Affiliated Hosp 1, Hangzhou 310006, Peoples R China.;[Jin, Weiyang] Hangzhou Normal Univ, Sch Life & Environm Sci, Hangzhou 311121, Peoples R China.;[Shen, Li] China Acad Chinese Med Sci, Inst Basic Theory TCM, Beijing 100700, Peoples R China.
通讯机构：
[Shen, Li] C;China Acad Chinese Med Sci, Inst Basic Theory TCM, Beijing 100700, Peoples R China.
摘要：
Objective: To observe the efficacy of Actinidia eriantha polysaccharide (ALPS) combined with PD1 antibody therapy in colorectal cancer-xenograft mice. Methods: CT26 cells were inoculated into 80 C57BL/6 mice to establish the colorectal cancer xenograft-mouse model. Mice were divided evenly into a model group, AEPS group, anti-PD1 group, and combined group. AEPS 5mL/kg.day was given orally and 10 mg/kg anti-PD1 injected intravenously for 28 days. Tumor growth and mouse survival were observed. Tumor-cell proliferation and metastasis markers Ki67, N-cadherin, KLF4, and Oct4 were detected with immunochemistry and Western blotting, T-cell infiltration in spleens and tumors was detected with MTT and flow cytometry. IFN gamma and TNF alpha were detected with ELISA. Results: Tumor growth was significantly retarded and survival prolonged in the AEPS, anti-PD1, and combined groups. Ki67 expression decreased in the anti-PD1 and combined groups, and N-cadherin, KLF4, and Oct4 expression decreased in the AEPS and combined groups. IFN gamma and TNF alpha levels, T-cell infiltration in spleen, and tumor all increased distinctively in the AEPS and combined groups. The combined group showed better antitumor effects and life-extension effect than the other two groups. Conclusion: AEPS and PD1 antibody-combination therapy can suppresses tumor growth and prolong survival of colorectal cancer-xenograft mice by regulating immunofunction, and the combined therapy showed better therapeutic efficacy than the single treatment.
通讯机构：
[Liang Li; Shucheng Zou] D;Department of Neurosurgery, Hunan Brain Hospital, Clinical Medical School of Hunan, University of Chinese Medicine, Changsha, 410007, Hunan Province, China.;Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, 410208, Hunan Province, China.
摘要：
Glioma is highly lethal because of its high malignancy. Ubiquitination, a type of ubiquitin-dependent protein modification, has been reported to play an oncogenic or tumor-suppressive role in glioma development, depending on the targets. Ring finger protein 139 (RNF139) is a membrane-bound E3 ubiquitin ligase serving as a tumor suppressor by ubiquitylation-dependently suppressing cell growth. Herein, we firstly confirmed the abnormal downregulation of RNF139 in glioma tissues and cell lines. In glioma cells, ectopic RNF139 overexpression could inhibit, whereas RNF139 knockdown could aggravate the aggressive behaviors of glioma cells, including hyperproliferation, migration, and invasion. Moreover, in two glioma cell lines, RNF139 overexpression inhibited, whereas RNF139 knockdown enhanced the phosphorylation of phosphatidylinositol 3-kinase (PI3K) and AKT serine/threonine kinase 1 (AKT). In a word, we demonstrate the aberration in RNF139 expression in glioma tissue samples and cell lines. RNF139 serves as a tumor-suppressor in glioma by inhibiting glioma cell proliferation, migration, and invasion and promoting glioma cell apoptosis through regulating PI3K/AKT signaling.
作者机构：
[兰晓栋; 罗晓欣; 金梦雨; 俞赟丰; 冯宇; 周曼丽] College of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China;National Key Discipline of TCM Diagnositics, Hunan Provincial Key Laboratory, Hunan University of Chinese Medicine, Changsha, 410208, China;[简维雄] College of Traditional Chinese Medicine, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China, National Key Discipline of TCM Diagnositics, Hunan Provincial Key Laboratory, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Jian, W.-X.] C;[Jian, W.-X.] N;National Key Discipline of TCM Diagnositics, China;College of Traditional Chinese Medicine, China
