作者机构：
[Xiao-rong CHANG] College of Acupuncture and Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学针灸推拿学院, 长沙410007, 中国);[Xiao-jun WANG] Hospital of Traditional Chinese Medicine, Xiangxi Tujia and Miao Autonomous Prefecture, Xiangxi Autonomous Prefecture 416000, China (湘西土家族苗族自治州民族中医院, 湖南湘西自治州416000, 中国);[Yi LIU; Jian XIONG; Bin HU] Changsha Wanze Health-care Management Co., LTD. Jiahua Outpatient Department, Changsha 410114, China (长沙万泽健康医疗管理有限公司嘉华城门诊部, 长沙410114, 中国);[Xin PEI; Xiang-ning SHAO; Xiao-wen TAN] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学中西医结合学院, 长沙410007, 中国);The Second Affiliated Hospital of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Liuyang 410300, China (湖南中医药大学附属第二中西医结合医院,湖南浏阳410300, 中国)
通讯机构：
[Xiao-wen TAN] C;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410007, China (湖南中医药大学中西医结合学院, 长沙410007, 中国)
摘要：
Based on the pathophysiological analysis of delayed-onset muscle soreness， the key points of its treatment was summarized in this paper: promoting the repair of injury， improving the metabolic state， and effectively relieving pain. Its key objectives and order of treatment are made clear: first to promote repair， second to improve metabolism， and then to relieve pain. And through the analysis of the key points of the treatment， the law of acupuncture and moxibustion treatment is put forward as follows: puncture those acupoints with the overall regulation function to adjust the secretion of endogenous analgesic substances in the body， at the same time， the special respiratory movement is adopted to improve the metabolic state， and the proper exercises are combined to promote the tissue repair.
作者机构：
Department of Traditional Chinese Medicine and Shenzhen Research Institute, Xiamen University, Xiamen, 361005, China;College of Acupuncture and Moxibustion, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122, China;College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China;School of Life Sciences, Xiamen University, Xiamen, 361005, China
通讯机构：
Department of Traditional Chinese Medicine and Shenzhen Research Institute, Xiamen University, Xiamen, China
通讯机构：
[Weihong Jiang] D;Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, People’s Republic of China<&wdkj&>Hunan Province Key Laboratory of Otolaryngology Critical Diseases, Xiangya Hospital of Central South University, Changsha, People’s Republic of China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital of Central South University, Changsha, People’s Republic of China
摘要：
PURPOSE: Subcutaneous immunotherapy (SCIT) is an effective treatment for pediatric allergic rhinitis (AR), but its efficacy fluctuates among individuals. This study aims to identify the profile of serum exosomes derived microRNAs (miRNAs) and evaluate their capacities to early predict SCIT efficacy in pediatric AR. PATIENTS AND METHODS: High-throughput sequencing was applied to identify the miRNA of serum exosomes in AR children. GO enrichment and KEGG pathway analysis were performed to enrich the biological annotations of target mRNAs of miRNAs. Then we validated differentially expressed miRNAs in two independent cohorts by RT-qPCR. Logistic regression and receiver operating characteristic curve (ROC) were applied to evaluate the abilities of identified miRNAs in predicting the efficacy of SCIT in AR children. RESULTS: A total of 812 miRNAs were detected in the serum exosomes, including 16 upregulated and 14 downregulated. Differentially expressed genes are enriched in the biological process of developmental process and regulation of cellular process, and gathered in pathways such as the signaling pathways regulating pluripotency of stem cells and the Wnt signaling pathway. In the first validation cohort, hsa-miR-4669 (P=0.009) and hsa-miR-4686 (P=0.032) were significantly downregulated in the effective group than the ineffective group, while hsa-miR-3196 (P=0.015) was upregulated. In the second cohort, hsa-miR-4669 level (P<0.0001) was downregulated in the effective group than the ineffective group. In addition, logistic regression revealed that hsa-miR-4669 level was correlated with the visual analogue scale (r=0.323, P=0.001) and total nasal symptoms score (r=0.269, P =0.007). ROC curve highlighted that hsa-miR-4669 level exhibited a reliable accuracy in predicting SCIT efficacy in pediatric AR (AUC=0.785). CONCLUSION: Serum exosomes derived miRNA were associated with the efficacy of SCIT. Serum exosomes derived hsa-miR-4669 might serve as a novel biomarker for early predicting the response of SCIT in AR children.
作者机构：
[Wei Zhou; Ming-Yu Li; Lan Zhou] College of Acupuncture and Massage,Hunan University of Traditional Chinese Medicine,Changsha 430100,China;[Li Liu] The First Affiliated Hospital of Hunan University of Traditional Chinese,Changsha 430100,China;[Mi Liu] Liuyang Chinese Medicine Hospital,Liuyang 430181,China
摘要：
Objective: To explore the rules of acupuncture point selection in thetreatment of rheumatoid arthritis by applying data mining and modern Statistical software.Methods: We retrieved literature from China hownet(CNKI), VIP database(VIP), Wanfang,PubMed, MEDLINE, Embase database from the founding of the database to February 1,2020. The main information of meridians and acupoints were entered into Excel, SPSS22.0 was used to extract data and do clustering analysis. Results: Quchi, Zusanli, Hegu, Yanglingquan, Xiyan were often chosen for electropuncture treatment of rheumatoid arthritis. Most of the acupoints are located in the large intestine channel of hand yangming bladder meridian or bladder channel of foot Taiyang, Shaoyang gallbladder channel of foot, stomachmeridian of foot Yangming, the energizer meridian and concentrated in the upper and lower extremities. The frequency of use of five Shu points, crossing point, source point, lowerconfluent and eight meeting points acupoints is higher. Cluster analysis can divide the included acupoints into 5 clusters. Conclusion: Quchi, Zusanli, Hegu, Yanglingquan, Xiyan are often chosen for electropuncture treatment of rheumatoid arthritis. Most of the selected acupoints aremostly distributed in the acupoints which are located in the large intestine channel of hand yangming bladder meridian or bladder channel of foot Taiyang, Shaoyang gallbladder channel of foot, Stomach meridian of foot Yangming, and the energizer meridian.
摘要：
Background Acupuncture is safe and effective for functional dyspepsia (FD), while its efficacy varies among individuals. Predicting the response of different FD patients to acupuncture treatment in advance and therefore administering the tailored treatment to the individual is consistent with the principle of predictive, preventive, and personalized medicine (PPPM/3PM). In the current study, the individual efficacy prediction models were developed based on the support vector machine (SVM) algorithm and routine clinical features, aiming to predict the efficacy of acupuncture in treating FD and identify the FD patients who were appropriate to acupuncture treatment. Methods A total of 745 FD patients were collected from two clinical trials. All the patients received a 4-week acupuncture treatment. Based on the demographic and baseline clinical features of 80% of patients in trial 1, the SVM models were established to predict the acupuncture response and improvements of symptoms and quality of life (QoL) at the end of treatment. Then, the left 20% of patients in trial 1 and 193 patients in trial 2 were respectively applied to evaluate the internal and external generalizations of these models. Results These models could predict the efficacy of acupuncture successfully. In the internal test set, models achieved an accuracy of 0.773 in predicting acupuncture response and an R-2 of 0.446 and 0.413 in the prediction of QoL and symptoms improvements, respectively. Additionally, these models had well generalization in the independent validation set and could also predict, to a certain extent, the long-term efficacy of acupuncture at the 12-week follow-up. The gender, subtype of disease, and education level were finally identified as the critical predicting features. Conclusion Based on the SVM algorithm and routine clinical features, this study established the models to predict acupuncture efficacy for FD patients. The prediction models developed accordingly are promising to assist doctors in judging patients' responses to acupuncture in advance, so that they could tailor and adjust acupuncture treatment plans for different patients in a prospective rather than the reactive manner, which could greatly improve the clinical efficacy of acupuncture treatment for FD and save medical expenditures.
作者机构：
[来奕恬; 周竞颖; 丁攀婷] Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China;[刘密; 李南; 张国山] College of Acupuncture-moxibustion-Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha 410208;[邱冉冉] The First Hospital of Hunan University of Chinese Medicine, Changsha 410007
作者机构：
[Shatong Z.; Xiaoyu L.; Tianhua Z.; Xing W.; Xiaojuan X.; Yan P.] College of Acupuncture and Moxibustion and Tui-na, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China
通讯机构：
[Yan, P.] C;College of Acupuncture and Moxibustion and Tui-na, China
