作者机构：
[熊家青] Graduate School of Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[刘丽芳; 李逵] Department of Breast Surgery, First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[张伟霞; 聂佳欣] Graduate School of Hunan University of Traditional Chinese Medicine, Changsha 410208
通讯机构：
Department of Breast Surgery, First Affiliated Hospital of Hunan University of Traditional, Chinese Medicine, Changsha, China
作者机构：
Graduate School, Hunan University of Chinese Medicine, Changsha, 410208;Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208;[张程程; 陈海交; 杨建文; 刘薇薇; 刘丽] Graduate School, Hunan University of Chinese Medicine, Changsha, 410208;[林亚平; 彭艳] Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Yan Peng] K;Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要：
Objective: To observe the effect of electroacupuncture (EA) at Zusanli (ST 36) on the gastrointestinal motility and the ultrastructures of pacemaker cells [the interstitial cells of Cajal (ICC)] in diabetic gastroparesis (DGP) rats and explore the mechanism of EA for DGP. Methods: A total of 50 Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, with 10 rats in each group. Group A was the blank control; a single intraperitoneal injection of 2% streptozotocin (STZ) was performed in rats of group B, group C, group D and group E, with high glucose and high fat diet for 8 weeks to establish the DGP rat models. Group B was the model group and the rats did not receive any treatment; group C was EA at acupoint group and the rats received EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group D was EA at non-acupoint group and the rats received EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6); group E was metoclopramide group and the rats were treated by intragastric administration of metoclopramide. Blood glucose was detected using ONE TOUCH blood glucose meter; gastric emptying rate and small intestine migration rate were measured using intragastric phenol red; ultrastructures of gastric antrum ICC were detected by transmission electron microscopy. Results: The differences of blood glucose between group B, group C, group D, group E versus that of group A were statistically significant after modeling (P<0.01); after treatment, the differences of blood glucose between group D, group E versus that of group C were statistically significant (P<0.05, P<0.01); the gastric emptying rate of rats in group B was statistically significant different from that in group A (P<0.01); the gastric emptying rate of rats in group C was statistically significant different from that in group B (P<0.01). The migration rates of rats’ small intestines in group B, group C, group D and group E were all statistically significant different from that in group A (P<0.01); the migration rate of rats’ small intestines in group C was statistically significant different from that in group B (P<0.01). The ultrastructure of rat’s ICC in group B showed apoptosis compared with that in group A; rat‘s ICC in group C had complete basement membrane, more cytoplasm mitochondria, Golgi and rough endoplasmic reticulum, showing clear structure, occasional mitochondria swelling and gap junctions with adjacent smooth muscle cells; there were no significant differences between group D, group E versus group B. Conclusion: EA at Zusanli (ST 36) plus other acupoints can regulate the blood glucose and promote gastrointestinal motility in DGP rats, and the mechanism may be related to repairing the damaged ICC structure.
作者机构：
[李莹; 潘诗敏; 李翠英; 张曦] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[李莹; 潘诗敏; 李翠英; 张曦] Graduate School of Hunan University of CM, Changsha 410208;[李金香] the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China. 1306550930@qq.com
作者机构：
[李玲] College of traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[高强; 卢芳国; 张波; 魏科] Medical College, Hunan University of Chinese Medicine, Changsha, 410208, China;[蔡亮] Microbiology Laboratory, Hunan Provincial Center for Disease Control and Prevention, Changsha, 410208, China;[张世鹰] Graduate School, Hunan University of Chinese Medicine, Changsha, 410208, China;[戴冰] Pharmacy college, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Lu, F.-G.] M;Medical College, Hunan University of Chinese Medicine, Changsha, China
作者机构：
School of Acupuncture,Moxibustion & Tuina,Hunan University of Chinese Medicine, Changsha, 410208;Postgraduate School of Hunan University of Chinese Medicine, Changsha, 410208;[李文慧; 谭杏; 宋洋] School of Acupuncture,Moxibustion & Tuina,Hunan University of Chinese Medicine, Changsha, 410208;[郁洁; 林亚平] Postgraduate School of Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Ya-ping Lin] P;Postgraduate School of Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective:To investigate the effects of electroacupuncture(EA)at Neiguan(PC 6)and Baihui(GV 20)by observing the changes of CCAAT/enhancer-binding protein(C/EBP)homologous protein(CHOP)and caspase-12 gene expressions in rats after cerebral ischemia-reperfusion injury(IRI),and explore whether the apoptosis pathway of endoplasmic reticulum stress(ERS)is involved in the protective mechanisms of EA.Methods:Sixty rats were randomly assigned to five groups(12 in each group):a normal control group(group A),a sham-operation group(group B),an operation group(group C),an Edaravone group(group D)and an EA group(group E).The cerebral IRI rat model was induced by middle cerebral artery occlusion(MCAO)using intraluminal monofilament.2,3,5-triphenyl tetrazolium chloride(TTC)staining was adopted in the measurement of cerebral infarction volume.Real-time polymerase chain reaction(RT-PCR)was used to determine the mRNA expressions of CHOP and caspase-12.Results:Compared with group A and group B,the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group C,group D and group E were increased,with statistical significances(P<0.05 or P<0.01);compared with group C,the volume of cerebral infarction and mRNA expressions of CHOP and caspase-12 in group D and group E were decreased significantly(P<0.05 or P<0.01);there were no significant differences between group D and group E in comparing the above items(P>0.05).Conclusion:EA at Neiguan(PC 6)and Baihui(GV 20)can effectively suppress the volume of cerebral infarction.Furthermore,the underlying mechanism of EA at Neiguan(PC 6)and Baihui(GV 20)is possibly related to the down-regulation of CHOP and caspase-12 mRNA expressions,so as to decrease cell apoptosis.
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China;[林亚平; 彭艳; 岳增辉] Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China
