作者机构：
[陈海交; 林亚平; 杨建文; 张程程; 彭艳; 刘薇薇; 刘丽; 刘密; 岳增辉] Hunan University of Chinese Medicine, Changsha, 410208
通讯机构：
[Yan Peng] H;Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Specificity of Acupoints;Point Selection;Diabetes Complications;Gastroparesis;Rats
摘要：
Objective: To observe the effect of electroacupuncture (EA) on the electrogastrogram and gastric antrum ghrelin in rats with diabetic gastroparesis (DGP). Methods: Fifty Sprague-Dawley (SD) rats were randomly divided into group A, group B, group C, group D and group E, 10 rats in each group. Group A was the blank control group without intervention. Group B, Group C, Group D and Group E were treated with single dose intraperitoneal injection of 2% streptozotocin (STZ), combined with 8-week high glucose and high fat diet to establish DGP rat models. Group B was the model group without treatment. Group C was the EA at acupoint group, was treated with EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Group D was the EA at non-acupoint group, was treated by EA at the control points of Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6). Rats in the metoclopramide control group received 1.7% metoclopramide solution [10 mL/(kg·bw)] by gavage. Rat's blood glucose level was measured by blood glucose meter; gastric emptying rate was detected using phenol red as a marker; the electrogastrogram was detected by BL-420F biological function system; the protein level of ghrelin was detected by enzyme-linked immunosorbent assay (ELISA); the expression of ghrelin mRNA was detected by real-time polymerase chain reaction (RT-PCR). Results: Compared with group A, the blood glucose of group B, C, D and E were significantly increased before and after the treatment (all P<0.01); after treatment, the gastric emptying rate of group B was significantly decreased (P<0.01), the migration rates of small intestine in group B, C, D and E were significantly lower (all P<0.01), and the protein content of ghrelin in group C was significantly decreased (P<0.01); the expressions of ghrelin mRNA were significantly increased in group B, C, D and E (all P<0.01), the mean amplitudes of electrogastrogram in group B and D were significantly decreased (both P<0.01). After treatment, compared with group B, the blood glucose of group C was significantly decreased (P<0.05), the gastric emptying rate and small intestine migration rate were significantly increased in group C and E (P<0.05, P<0.01), the small intestinal migration rate was significantly increased in group D (P<0.05), the expression of ghrelin in protein and mRNA in group C was significantly lower (P<0.01), the expression of ghrelin mRNA in group E was significantly lower (P<0.05), and the mean amplitude of electrogastrogram in group C was significantly increased (P<0.05). After treatment, compared with group D, the protein and mRNA expressions of ghrelin in group C were significantly decreased (P<0.01). After treatment, compared with group E, the protein expression of ghrelin in group C was significantly decreased (P<0.01). Conclusion: EA at Zusanli (ST 36), Liangmen (ST 21) and Sanyinjiao (SP 6) could regulate the blood glucose level of DGP model rats, enhance electrogastrogram activity, promote gastric emptying, and regulate ghrelin expression in protein and mRNA.
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com
作者机构：
[彭艳; 林亚平; 贺凤娥; 万荃荃; 陈文; 刘琴; 易受乡] Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha, 410007
通讯机构：
[Ya-ping Lin] M;Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of Traditional Chinese Medicine, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective: To explore the action mechanism of electroacupuncture (EA) in improving the gastric motility of rats with diabetic gastroparesis (DGP). Methods: Forty-eight healthy Sprague-Dawley (SD) rats were randomly assigned into four groups: a normal group (group A), a model group (group B), a group of EA at acupoints (group C), and a group of EA at non-acupoints (group D), 12 rats in each group. The animal model of DGP was established by intraperitoneal injection of streptozotocin (STZ) plus high glucose and fat diet. The blood glucose, urine glucose and gastric emptying rate (GER) were observed. The content of insulin (INS) in serum and ghrelin in gastric antrum tissue were detected by enzyme linked immunoassay (ELISA). The expression of growth hormone secretagogue receptor mRNA (GHSR mRNA) in gastric antrum tissue was detected by real-time fluorescent quantitative polymerase chain reaction (PCR). Results: Compared with group A, blood glucose and urine glucose increased significantly (P<0.01), GER, content of serum insulin, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue decreased significantly (P<0.05 or P<0.01) in group B. Compared with group B, blood glucose and urine glucose decreased significantly (P<0.05), GER, the content of insulin in serum, the content of ghrelin and expression of GHSR mRNA in gastric antrum tissue increased significantly (P<0.05 or P<0.01) in group C. Conclusion: EA at acupoints can down-regulate the content of blood and urine sugar, and promote gastric emptying, which is possibly related to the regulation of serum insulin, and the expressions of ghrelin and GHSR mRNA in gastric antrum.
作者机构：
[彭艳; 刘丽; 刘密; 吴雪芬; 郭鑫; 岳增辉; 郑雪娜] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China;[袁建菱] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China. 624755064@qq.com
摘要：
目的：观察电针对糖尿病胃轻瘫(DGP)大鼠血清胰岛素(INS)水平和胃窦组织胆囊收缩素(CCK)含量的影响。方法将60只SD大鼠随机分为A组、B组、C组、D组和E组,每组12只。A组为正常对照组,B组、C组、D组和E组采用腹腔一次性注射2%链脲佐菌素(STZ)配合高糖高脂不规则饮食建立DGP模型。B组为模型组,不接受治疗；C组采用电针足三里、三阴交和梁门治疗；D 组采用电针足三里、三阴交和梁门的对照点治疗；E 组采用甲氧氯普胺片(胃复安)药液灌胃治疗。采用OneTouch 血糖仪测血糖,尿糖试纸测尿糖,酚红灌胃法测胃排空率和肠移行率,并采用 ELISA 法检测各组大鼠治疗前后血清INS水平和胃窦组织CCK的含量。结果 B组、C组、D组和E组大鼠血糖值及尿糖值与A组比较,差异均具有统计学意义(P＜0.01)。C组大鼠血糖值及尿糖值与B组和E组比较,差异均具有统计学意义(P＜0.05)。B组、C组、D组和E组大鼠胃排空率与 A 组比较,差异均具有统计学意义(P＜0.01)。C 组大鼠胃排空率与 B 组和 D 组比较,差异均具有统计学意义(P＜0.01,P＜0.05)。B组和D组大鼠肠移行率与A组比较,差异均具有统计学意义(P＜0.01)。C组大鼠肠移行率与B组和D组比较,差异均具有统计学意义(P＜0.01)。B组大鼠血清INS水平及胃窦组织CCK含量与A组比较,差异均具有统计学意义(P＜0.05,P＜0.01)。D组大鼠胃窦组织CCK含量与A组比较,差异具有统计学意义(P＜0.01)。C组和E组大鼠血清INS水平及胃窦组织CCK含量与B组比较,差异均具有统计学意义(P＜0.05,P＜0.01)。C组大鼠胃窦组织CCK含量与D组比较,差异具有统计学意义(P＜0.05)。结论电针能明显改善DGP大鼠的胃肠功能症状,促进胃排空,其疗效可能与电针刺激能提高血清INS水平,同时降低胃窦组织CCK含量有关。
期刊：
Journal of Acupuncture and Herbs,2015年3(1):70-76
作者机构：
Graduate School, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China Key Acu-Moxibustion Lab. of Biological Information Analyzing, Institute of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha, 410208, China
关键词：
Life Sciences;Life Sciences, other;Medicine;Clinical Medicine;Complementary Medicine;Pharmacy;Pharmacy, other
摘要：
Objective: To observe the effects of electro-acupuncture (EA) on ultrastructure of gastric antrum smooth muscle cells (GASMCs) in diabetic gastroparesis (DGP) model rats, and to explore the possible mechanism underlying the therapeutic effects of EA.
期刊：
Journal of Acupuncture and Herbs,2015年3(1):38-45 ISSN：2001-7464
作者机构：
Key Research Lab. on Acupoint-organ Relations and Key Lab. of Bioinformatic Analysis on Acupuncture-moxibustion, Acupuncture-Moxibustion and Tuina Massage School, Hunan University of Traditional Chinese Medicine, Changsha, 417000, China Phisiotherapy Department, Hunan People’s Hospital, Changsha, 410005, China
关键词：
Life Sciences;Life Sciences, other;Medicine;Clinical Medicine;Complementary Medicine;Pharmacy;Pharmacy, other
摘要：
Objective: to observe the effects of moxibustion on histomorphological changes of gastric mucosa, as well as on serum IL-6、IL-8、TNF-α，Hp IgG、CD Methods: 40 SD rats were randomly assigned to four groups: group A (blank control), group B (Hp infection model), group C (moxibustion plus model), group D (electro-acupuncture plus model), 10 for each group. The “NaHCO Results: compared with group A, the values of IL-6, IL-8, TNF-α, Hp IgG and CD Conclusion: the preventive and therapeutic effects of moxibustion on Hp related gastritis might be realized by two ways- to inhibit the secretion of proinflammatory cytokines such as IL-6, IL-8 and TNF-α, or to regulate the production of immune factors (such as up-regulation of CD
