作者机构：
[Dong-Feng Z.; Xing W.; Xiao-Juan X.; Ya-Ping L.; Yan P.] The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
通讯机构：
[Lin Ya-Ping] T;The Domestic First-class Discipline Construction Project of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
期刊：
Canadian Journal of Gastroenterology and Hepatology,2020年2020:1-10 ISSN：2291-2789
通讯作者：
Peng, Y
作者机构：
[Wei, Xing; Xiao, Xiaojuan; Peng, Yan; Peng, Y; Zhao, Dongfeng; Lin, Yaping] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Domest First Class Discipline Construct Project C, Changsha 410000, Peoples R China.;[Chen, Qiao; Chen, Siyi] Hunan Univ Chinese Med, Sch Med, Changsha 410000, Peoples R China.
通讯机构：
[Peng, Y ] H;Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Domest First Class Discipline Construct Project C, Changsha 410000, Peoples R China.
摘要：
<jats:p><jats:italic>Background</jats:italic>. The incidence of diabetic gastroparesis (DGP) is mainly blamed to abnormity of interstitial cells of Cajal (ICCs). Autophagy could degrade damaged proteins and organelles to keep intracellular homeostasis, and it could directly influence structure and number of cells. In this study, we aimed to figure out the relationship between DGP and autophagy of ICCs. <jats:italic>Methods</jats:italic>. Sixty Sprague-Dawley (SD) rats were randomly divided into normal control group (NC, 10) and modeling group (50). Rats in the modeling group were injected 2% streptozotocin (STZ) and fed with high-glucose and high-fat diet for 8 weeks in order to establish DGP rat model. After modeling, 30 successfully modeled rats were randomly selected and separated into diabetic gastroparesis group (DGP, 10), GDP rats with electroacupuncture group (EA, 10), and GDP rats with metoclopramide group (MP, 10). When the intervention was completed, blood glucose was measured by ONE TOUCH glucometer and gastrointestinal propulsive rate was detected through measuring optical density. Autophagosomes were observed under transmission electron microscope (TEM). The expression of LC3 protein and P62 protein was measured by Western blot. When ICCs were transfected with GFP-RFP-LC3 plasmid, autophagy flux was observed by laser scanning confocal microscope. <jats:italic>Results</jats:italic>. (1) After intervention, compared with blood glucose of rats in the NC group, all of the DGP, EA, and MP groups were remarkably increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the DGP group, the blood glucose of the EA and MP groups was decreased greatly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (2) Compared with gastrointestinal propulsive rate of rats in the NC group, no matter gastric emptying rate or intestinal propulsive rate, the EA and MP groups were significantly reduced (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the NC group, gastric emptying rate and intestinal propulsive rate in the EA group were obviously decreased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>); compared with the DGP group, the EA and MP groups were increased significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (3) Compared with the NC group, intensity of RFP and GFP in the DGP group was obviously increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M8"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>), in other words, the DGP group accompanying suppression of autophagy; compared with the DGP group, intensity of RFP and GFP in the EA group was decreased significantly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M9"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M10"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (4) There was no autophagosome in the NC group, and an autophagosome existed in the DGP group. Both EA and MP groups found autophagy. (5) When coming to LC3 II/LC3 I, compared with the NC group, the ratio was enhanced in the DGP and EA groups (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M11"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>, <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M12"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:mrow></mml:math>); compared with the DGP group, LC3 II/LC3 I was dramatically decreased in the MP and EA groups (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M13"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). (6) As the substrate of degradation, the expression of P62 in the other three groups was significantly increased (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M14"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>) compared with the NC group; compared with the DGP group, the amount of P62 in the EA and MP groups was reduced greatly (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M15"><mml:mrow><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn>0.01</mml:mn></mml:mrow></mml:math>). <jats:italic>Conclusion</jats:italic>. The impaired autophagy flux in ICCs is the pathological basis of diabetic gastroparesis, blaming to fusion dysfunction of autophagosome and lysosome and electroacupuncture (EA) could ease the suppression of autophagy to improve gastric motility.</jats:p>
摘要：
<jats:title>Abstract</jats:title><jats:p>Cerebral ischemia/reperfusion (I/R) injuries are common and often cause severe complications. Ozone has been applied for protecting I/R injury in animal models of several organs including cerebra, but the detailed mechanism remains unclear. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and lactate dehydrogenase measurement were used to determine the influence of ozone on cell activity and damage of SH‐SY5Y cells. Some redox items such as catalase (CAT), malondialdehyde (MDA), glutathione peroxidase (GSH‐Px), and superoxide dismutase (SOD) were measured by enzyme‐linked immunosorbent assay. The mitochondrial membrane potential (Δ<jats:italic>Ψ</jats:italic><jats:sub>m</jats:sub>) was determined by JC‐1 assay. Cytochrome‐c (cyt‐c) level in the cytoplasm and mitochondrion was measured by western blotting. Apoptosis was determined by flow cytometry, and some apoptosis‐related molecules were detected by quantitative real‐time polymerase chain reaction and western blotting. Ozone alleviated oxidative damage by increasing GSH‐Px, SOD, CAT, and decreasing MDA. Ozone decreased mitochondrial damage caused by I/R injury and inhibited the release of cyt‐c from mitochondrion to cytoplasm in SH‐SY5Y cells. The cell apoptosis caused by I/R was inhibited by ozone, and ozone could decrease apoptosis by increasing the ratio of Bcl‐2/Bax and inhibiting caspase signaling pathway in SH‐SY5Y cells. Ozone has the ability of maintaining redox homeostasis, decreasing mitochondrion damage, and inhibiting neurocytes apoptosis induced by I/R. Therefore, ozone may be a promising protective strategy against cerebral I/R injury.</jats:p>
作者：
Liu Mi;Liu Yi;Liu Wei-wei;Wang Chao;Tan Cheng-fu;...
期刊：
针灸推拿医学：英文版,2018年16(4):216-222 ISSN：1672-3597
通讯作者：
Xiao-rong Chang<&wdkj&>Jing Li
作者机构：
[Liu Yi; Liu Wei-wei; Wang Chao; Tan Cheng-fu; Liu Li; Peng Yan; Chang Xiao-rong; Yan Jie] Hunan University of Chinese Medicine, Changsha, 410208, China;Liuyang Hospital of Chinese Medicine, Liuyang, Hunan Province 410300, China;[Li Jing] Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China;[Liu Mi] Hunan University of Chinese Medicine, Changsha, 410208, China<&wdkj&>Liuyang Hospital of Chinese Medicine, Liuyang, Hunan Province 410300, China
通讯机构：
[Xiao-rong Chang] H;[Jing Li] Y;Hunan University of Chinese Medicine, Changsha, China<&wdkj&>Yueyang Hospital of Integrated Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China
摘要：
Aims: The expression of phosphoglycerate kinase 1 (MMP19) is elevated in some cancers. However, the clinical features and prognostic value of glioma patients with MMP19 expression are unclear. In this study, the expression level of MMP19 and the correlation between the level of MMP19 expression and the clinicopathologic data in glioma patients including survival were examined. Methods and results: Using real-time PCR, the mRNA expression of MMP19 was examined in 61 fresh glioma tissues and 32 brain samples. The result indicated that MMP19 mRNA was obviously elevated in glioma tissues compared to brain tissues. Further, we observed that MMP19 mRNA was much higher in stage III patients than it was in stage I-II patients. The expression of the MMP19 protein was determined by immunohistochemical analysis in 156 paraffin-embedded glioma samples and 35 normal paraffin-embedded brain samples. The MMP19 protein level was significantly increased in glioma tissues compared to brain tissues (P = 0.008). Furthermore, we observed that a high expression of MMP19 protein was positively associated with clinical stage (P = 0.008) but did not correlate with age, gender, or histological type. An increased MMP19 protein expression was associated with poor overall survival rates (P = 0.001). A stratified analysis showed that patients with high MMP19 protein expression indicated a worse prognosis occurring in WHO III-IV stages (P = 0.001). A Multivariate analysis indicated that a high expression of the MMP19 protein was an independent prognostic indicator of patient survival (P = 0.009). Conclusions: MMP19 is overexpressed and plays a significant role in disease progression and poor outcome in glioma patients.
作者机构：
[张程程; 陈海交; 杨建文; 刘薇薇; 刘丽] Graduate School, Hunan University of Chinese Medicine, Changsha, 410208, China;[林亚平; 彭艳] Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Yan Peng] K;Key Laboratory of Acupuncture Bioinformation Analysis, School of Acupuncture, Moxibustion & Tuina, Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Electroacupuncture;Interstitial Cells of Cajal;Diabetes Complications;Gastroparesis;Rats
摘要：
Novel organobismuth(III) complex of 5H-dibenzo[c,f][1,5]oxabismocin-12(7H)-yl nitrate (C2) was synthesized and characterized by spectral and elemental analysis. It was compared with other five C,E,C-chelating (E = N, O, S) organobismuth(III) complexes against human adenocarcinoma alveolar basal epithelial cells (A549), human liver cancer cell line (SMCC7721), human gastric cancer cell line (SGC-7901), human colon adenocarcinoma cell line (SW480) and healthy human bronchial cell line (16HBE14o-) in vitro. It was found that C2 exhibited the best anticancer activity. Further mechanistic investigation indicated that toxicological activity of C2 was ascribable to apoptosis rather than anti-proliferative activity. Apoptosis was induced through up-regulating the level of Bcl-2/Bax as well as the activation of caspase-3. The results demonstrate that heterocyclic organobismuth(III) complexes of this type have great potential in the treatment of cancer.
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China;[林亚平; 彭艳; 岳增辉] Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China
