作者机构：
College of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[蔺婷; 胡晶] Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, 410208, China;[何迎春; 周芳亮; 胡梅] College of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Engineering and Technological Research Center for Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Chinese Medicine and Protecting Visual Function, Changsha, 410208, China
通讯机构：
[He, Y.-C.] C;College of Medicine, Hunan University of Chinese Medicine, Changsha, China
作者机构：
Post-Graduate School, Hunan University of Chinese Medicine, Changsha, 410208, China;Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[胡梅] College of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;Hunan Provincial Research Engineering Technology Center for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine and Visual Function Protection, Hunan University of Chinese Medicine, Changsha, 410208, China;College of traditional Chinese Medicine, Changsha Medical University, Changsha, 410219, China
通讯机构：
[He, Y.] H;Hunan Provincial Key Lab for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China
关键词：
益气解毒方;鼻咽癌;细胞凋亡;MAPK/ERK信号通路
摘要：
目的 研究益气解毒方水提物对鼻咽癌细胞凋亡的影响,并从MAPK/ERK信号通路探讨其诱导凋亡的作用机制。方法 CCK-8法检测益气解毒方水提物对CNE1、CNE2细胞增殖的影响;Hoechst 33342染色法、JC-10染色法、荧光双染流式细胞仪检测其对CNE1、CNE2细胞凋亡的影响;Western blot法检测其对CNE1、CNE2细胞蛋白表达的影响。结果 益气解毒方水提物能抑制CNE1、CNE2细胞增殖( P <0.05)、诱导凋亡( P <0.05);药物作用48 h后,Survivin、XIAP、Bcl-2表达下降,Bax表达上升,MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK表达下降( P <0.05);在此基础上,加入激活剂ISO和益气解毒方水提物后,与单用益气解毒方水提物相比,p-c-Raf、p-MEK、p-ERK1/2表达上调,Survivin、XIAP、Bcl-2表达增加,Bax表达下降,促凋亡效应也降低( P <0.05)。结论 益气解毒方水提物可诱导鼻咽癌细胞凋亡,该效应与其抑制MAPK/ERK信号通路关键蛋白p-c-Raf、p-MEK、p-ERK1/2表达,进而下调Survivin、XIAP、Bcl-2表达,上调Bax表达有关。
