作者机构：
[Qin, Li-Feng; Peng, Dan; Zhang, Qing] Cent S Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410010, Hunan, Peoples R China.;[Qin, Li-Hua] Hunan Univ Chinese Med, Sch Nursing, Dept Gynecol, Changsha 410208, Hunan, Peoples R China.;[Xu, Min] Cent S Univ, Xiangya Hosp 2, Intens Care Unit, Changsha, Hunan, Peoples R China.;[Fang, Han] Cent S Univ, Xiang Ya Hosp, Dept Cardiovasc Dis, Changsha 410008, Hunan, Peoples R China.;[Zhang, Qing] Cent S Univ, Xiangya Hosp 2, Dept Orthoped, 139 Ren Min Rd, Changsha 410010, Hunan, Peoples R China.
通讯机构：
[Zhang, Qing] C;Cent S Univ, Xiangya Hosp 2, Dept Orthoped, 139 Ren Min Rd, Changsha 410010, Hunan, Peoples R China.
关键词：
S2 nerve root;chordoma;giant cell tumor;rectum;sacral tumor;urinary and bowel function
摘要：
The current report describes the case of a 29-year-old female with a sacral giant cell tumor (GCT) during pregnancy. Originally, the patient presented with severe pain in the lumbosacral region, radiating posterolaterally from the lumbar spine into the bilateral thigh and subsequently, into the bilateral crus posterolaterally. Plain X-rays, computed tomography and magnetic resonance imaging showed osteolytic destruction of the sacrococcygeal bones and a huge soft-tissue mass with features of a chordoma. The patient underwent a partial en bloc sacrectomy (partial S1 and completely below) and curettage for tumors located at the sacroiliac joint and underlying left ilium, with bilateral internal iliac arteries ligated to control intraoperative hemorrhage. The patient's bilateral S2 nerve roots were killed. The diagnosis of conventional GCT was determined based on the histopathological examination of the resected specimen. Urinary and bowel functions were recovered by exercising.
关键词：
Single Nucleotide Polymorphism;tagSNPs;Genetic Algorithm;Artificial Neural Network
摘要：
Currently, many approaches have been developed to be applied in the tagSNP selection research. However, there are still drawbacks existing in these methods, manifested chiefly by high time complexity, large number of selected tagSNPs, low prediction accuracy and inefficient tagSNPs in followup study. We propose an informative SNP selection method framework based on genetic algorithm in this paper to address these problems. In this study, we separately improve the phases of informative SNPs set construction and haplotypes reconstruction. Firstly, we eliminate the large number of redundant SNPs with LD values to obtain a candidate subset with small redundancy, and then seek for optimization with genetic algorithm not only to ensure the reconstruction accuracy efficiently but also to reduce time complexity greatly. Besides, to avoid retrain prediction model repeatedly by traditional methods (e.g., MLR, SVM and so on), we make full use of BP neural network's multiple output characteristics to reconstruct all non-tagSNP at once. Thus, it significantly saves computational complexity. The experimental results show that our method performs much better than the current dominating tagSNP selection methods.
摘要：
Ethnopharmacological relevance: The root bark of Lycium chinense Miller, Lycii Radicis Cortex, has been used in traditional Chinese medicine (TCM) to treat different inflammation-related symptoms, such as diabetes mellitus. The pro-inflammatory transcription factor nuclear factor kappa B (NF-kappa B) is a key regulator of inflammation, while the transcription factor peroxisome proliferator-activated receptor gamma (PPAR gamma) is. a key modulator of genes involved in diabetes development. To identify putative active compound(s) from Lycii Radicis Cortex inhibiting NF-kappa B or activating PPAR gamma. Material and methods: Using activity-guided fractionation, six extracts with different polarity, isolated fractions, and purified compounds from Lycii Radicis Cortex were tested for NF-kappa B inhibition and PPAR gamma activation in vitro. The structure of the purified compounds was elucidated by NMR and MS techniques. Results: The ethyl acetate extract and the methanol extract of Lycii Radicis Cortex suppressed tumor necrosis factor alpha (TNF-alpha)-induced activation of NF kappa B, while the dichloromethane extract activated PPAR gamma. Nine phenolic amide analogues, including trans-N-(p-coumaroyl)tyramine (1), trans-N-feruloyltyramine (2), transN-caffeoyltyramine (3), dihydro-N-caffeoyltyramine (4), three neolignanamides (5-7), and two lignanamide (8, 9), were isolated and their inhibitory potential on NF-kappa B was determined (1-4 were also contained in water decoction). Two of the nine isolated phenolic amides inhibited TNF-alpha-induced NF-kappa B activation. Trans-N-caffeoyltyramine was verified as the key component responsible for the NF-kappa B inhibition with an IC50 of 18.4 mu M in our cell-based test system. Activation of PPAR gamma was attributed to a palmitic-acid enriched fraction which displayed concentration-dependent effect ablated upon co-treatment with the PPAR gamma antagonist T0070907. Conclusions: Phenolic amides were confirmed as main components from Lycii Radicis Cortex responsible for NF-kappa B inhibition. Fatty acids were identified as the major plant constituent responsible for the PPAR gamma activation. Structure-activity relationship analysis suggests that the NF-kappa B inhibitory activity of trans-N-caffeoyltyramine may be attributed to its Michael acceptor-type structure (alpha,beta-unsaturated carbonyl group). The data of this study contribute to a better understanding of the molecular mechanism of action of Lycii Radicis Cortex extracts in the context of inflammation. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
关键词：
EEG;Synchronization;Brain Functional Integration;Theoretic Measures;Hilbert transform;Phase Locked Value
摘要：
Synchronization is one of the basic characteristics of EEG, which is of great significance to the study of EEG. But the synchronous evaluation methods of EEG are calculated according to local EEG, it reflects the local state of brain without considering the overall activity of the brain, so this is important for a new synchronous evaluation index of the overall state of the brain. Based on the information theory, this article gives a process of synchronization index from the calculation of ECoG. Using the process, the experimental gets the index IRAA and IMA2 as evaluation index of EEG global synchronization. In order to assess the decision performance of index to the EEG synchronization, carrying out the 3 groups experiment based on the EEG collected in several cognitive tasks. The experimental results show that: (1) the signal processing process proposed is effective to EEG, which are able to more clearly distinguish the EEG synchronization; (2) the IMA2 and IRAA is effective for EEG synchronization judgment; (3) Compared to the values of PLV, IMA2 and IRAA can be more clearly differentiate the synchronization state of EEG, reflecting the global brain activity.
摘要：
The Notch signaling pathway plays versatile roles during heart development. However, there is contradictory evidence that Notch pathway either facilitates or impairs cardiomyogenesis in vitro. In this study, we developed iPSCs by reprogramming of murine fibroblasts with GFP expression governed by Oct4 promoter, and identified an effective strategy to enhance cardiac differentiation through timely modulation of Notch signaling. The Notch inhibitor DAPT (N-[N-(3,5-difluorophenacetyl)-l-alanyl]-S-phenylglycine t-butyl ester) alone drove the iPSCs to a neuronal fate. After mesoderm induction of embryoid bodies initiated by ascorbic acid (AA), the subsequent treatment of DAPT accelerated the generation of spontaneously beating cardiomyocytes. The timed synergy of AA and DAPT yielded an optimal efficiency of cardiac differentiation. Mechanistic studies showed that Notch pathway plays a biphasic role in cardiomyogenesis. It favors the early-stage cardiac differentiation, but exerts negative effects on the late-stage differentiation. Therefore, DAPT administration at the late stage enforced the inhibition of endogenous Notch activity, thereby enhancing cardiomyogenesis. In parallel, DAPT dramatically augmented the expression of Wnt3a, Wnt11, BMP2, and BMP4. In conclusion, our results highlight a practicable approach to generate cardiomyocytes from iPSCs based on the stage-specific biphasic roles of Notch signaling in cardiomyogenesis.
期刊：
Journal of Computational and,2014年11(7):1649-1652 ISSN：1546-1955
作者机构：
湖南大学
关键词：
Single Nucleotide Polymorphisms;Weighted Fuzzy Kernel Clustering;Quality Control
摘要：
The quality of single nucleotide polymorphisms (SNPs) is of paramount importance for genome-wide association studies (GWAS) to reduce potential false findings. The SNP genotyping data are not always accurate because of various reasons such as experimental systematic errors. SNP quality control methods commonly use filter-by-extreme filters based on quality control variables of Hardy-Weinberg equilibrium (HWE), missing frequency (MiF) and minor allele frequency (MAF), to remove outliers. These filters neglect the fact that variables may contribute differently for different SNP clusters, and their implementation requires arbitrary thresholds. For this problem, a novel filtering method based on weighted fuzzy kernel clustering algorithm (WFKCA) is described to identify outlier SNPs.
期刊：
International Journal of Molecular Sciences,2014年5(15):8335-8351 ISSN：1422-0067
作者机构：
1.Division of Stem Cell Regulation and Application, College of Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, China
2.Department of Medical Microbiology, Immunology & Cell Biology, Simmons Cancer Institute, Southern Illinois University School of Medicine, 913 N, Rutledge Street, Springfield, IL 62702, USA
关键词：
cancer stem cells; side population; apoptosis; apoptotic inducers; apoptotic death pathways; and cancer therapy
摘要：
Cancer stem cells (CSCs) play crucial roles in tumor progression, chemo- and radiotherapy resistance, and recurrence. Recent studies on CSCs have advanced understanding of molecular oncology and development of novel therapeutic strategies. This review article updates the hypothesis and paradigm of CSCs with a focus on major signaling pathways and effectors that regulate CSC apoptosis. Selective CSC apoptotic inducers are introduced and their therapeutic potentials are discussed. These include synthetic and natural compounds, antibodies and recombinant proteins, and oligonucleotides.
摘要：
A bacteriocin-producing lactic acid bacteria, FX-6, was isolated from Tibetan kefir using the agar well diffusion assay. Strain FX-6 was identified as Lactobacillus paracasei subsp. tolerans on the basis of 16S rDNA sequence analyses. This bacteriocin, which was designated bacteriocin Fl, was purified by a threestep purification procedure. The molecular mass of bacteriocin Fl was 2113.842 Da by MALDI-TOF MS analyses. It was also discovered to have blocked N-termini, hampering analysis by Edman degradation. Bacteriocin Fl exhibited a wide range of antimicrobial activity, strong heat stability (20minat 121 C, or 60 min at 100 degrees C) and pH stability (pH 3.0-9.0). Following treatment by pepsin and trypsin, the antibacterial activity was partly reduced. Bacteriocin Fl is the first reported bacteriocin produed by L paracasei subsp. tolerans which can not only inhibit fungi but also bacteria. The characterization of bacteriocin Fl suggested that it was a novel bacteriocin with potential research and application value in food industry. (C) 2014 Elsevier Ltd. All rights reserved.
