期刊：
Journal of Atherosclerosis and Thrombosis,2011年18(9):796-807 ISSN：1340-3478
通讯作者：
Tang, Chao-Ke
作者机构：
[Tang, Chao-Ke; Li, Xiao-Xu; Hu, Yan-Wei; Liu, Xie-Hong; Tang, Ya-Ling; Mo, Zhong-Cheng; Yi, Guang-Hui; Wang, Zuo; Xiao, Ji] Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Peoples R China.;[Liao, Duan-Fang] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, State Key Lab Chinese Med Powder & Med Innovat Hu, Changsha, Hunan, Peoples R China.
通讯机构：
[Tang, Chao-Ke] U;Univ S China, Inst Cardiovasc Res, Key Lab Atherosclerol Hunan Prov, Life Sci Res Ctr, Hengyang 421001, Peoples R China.
关键词：
Advanced oxidation protein products;ATP-binding cassette transporter A1;JAK/STAT;Cholesterol efflux
摘要：
AIMS: Advanced oxidation protein products (AOPPs) are new independent risk factor for coronary artery disease. This study was to determine the effects and potential mechanisms of AOPPs on cholesterol efflux from human macrophage foam cells. METHODS: Human THP-1 monocytes were preincubated with Phorbol-12-myristate- 13-acetate (PMA) and oxidized low density lipoprotein (ox-LDL) to form foam cells. The protein and mRNA expression were examined by western immunoblotting assays and real-time quantitative PCR, respectively. Cellular cholesterol content was measured by HPLC. The cholesterol efflux was assessed by liquid scintillation counting. RESULTS: AOPPs significantly decreased the expression of ATP-binding membrane cassette transporter A-1 (ABCA1) and liver X receptor alpha (LXRalpha) and reduced cholesterol efflux from THP-1 macrophage- derived foam cells. AOPPs substantially activated NADPH oxidase and activated Janus kinase/signal transducers and activators of transcription (JAK/STAT) signal pathway in THP-1-derived foam-like cells. Inhibiting NADPH oxidase by diphenyliodonium (DPI) effectively abolished the AOPPs-induced decrease in cholesterol efflux and the expression of ABCA1. Inhibiting JAK/STAT activation by its specific inhibitor AG-490 or by siRNA could also block AOPPs action on THP-1 cells. CONCLUSIONS: AOPPs may first down-regulate the expression of LXRalpha and ABCA1 through JAK/STAT signal pathway activation and then inhibit cholesterol efflux in THP-1-derived foam-like cells; therefore, our study may be useful for understanding the critical effects of AOPPs on the pathogenesis of atherosclerosis.
摘要：
In the present paper, experimental materials of 33 accessions representative of the Euodia rutaecarpa from four Chinese provinces were analyzed using the sequence-related amplified polymorphism (SRAP) and amplified fragment length polymorphism (AFLP) techniques, focusing on their molecular discrimination and the assessment of their genetic relatedness. For the analysis, we optimized 10 pairs of SRAP primers and 6 pairs of AFLP primers, (The software package NTSYS-pc 2.1 was applied to analyze the data matrix) and cluster analysis distributed samples into two clusters, one with E. rutaecarpa var. officinalis (Dode) Huang and the other with E. rutaecarpa (Juss.) Benth by SRAP+AFLP markers in the same similarity coefficient of 0.53 (Genetic parameters also analyzed using POPGENE version 1.31). Genetic diversity in the species was detected with SRAP (H = 0.2260, I = 0.3341) and AFLP (H = 0.1665; I = 0.2518) markers. Genetic variability levels of E. rutaecarpa var. officinalis (Dode) Huang was higher than genetic variability levels of E. rutaecarpa (Juss.) Benth. Our study shown that both SRAP and AFLP molecular markers are in high efficiency in detecting the genetic diversity of E. rutaecarpa.
作者机构：
[Li, Guangcheng; Dong, Keli; Zhang, Ting; Zhu, Hong] Cent S Univ, Xiangya Hosp 3, Dept Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Zhang, Zhanwei] Hunan Univ Chinese Med, Hosp 1, Dept Neurosurg, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Dong, Keli] C;Cent S Univ, Xiangya Hosp 3, Dept Chinese Med, Changsha 410013, Hunan, Peoples R China.
关键词：
icariin;Alzheimer’s disease;entorhinal cortex;proteomics;traditional Chinese medicine;neural regeneration
摘要：
The present study sought to explore the mechanism of action by which icariin, an active component of Epimedii Herba, treats Alzheimer's disease at the proteomics level. Two-dimensional gel electrophoresis was used to isolate total protein from the entorhinal cortex of senescence-accelerated mouse prone 8 (SAMP8) mice, and differential protein spots were obtained. Corresponding peptide mass fingerprinting was conducted through mass spectrography to identify differential protein spots. Twenty-six differential protein spots were found in the entorhinal area of SAMP8 mice at 8 weeks following intragastric perfusion with icariin and double distilled water. Fourteen spots were identified, which were involved in mitochondrial energy metabolism, oxidative stress, and neuronal function. The results revealed that icariin can regulate the expression of various proteins in the entorhinal cortex of SAMP8 mice, and treat Alzheimer's disease by improving mitochondrial function, suppressing oxidative stress, inhibiting neural cell apoptosis, and protecting neurons.
摘要：
Previously, we found that G protein-coupled receptor APJ endogenous ligand apelin-13 stimulates vascular smooth muscle cells (VSMC) proliferation mediated in part by PKC-PI3K-ERK1/2-cyclinDl signaling cascades. In this study, Raf-1-14-3-3 signaling in rat VSMCs proliferation stimulated by apelin-13 was further investigated. Cell proliferation was measured with MTT assay. Expression of PI3K, phospho-PI3K, Raf-1, phospho-Raf-1, ERK1/2, phospho-ERKl/2, cyclinDl and cyclinE were detected by Western blotting. 14-3-3 protein combining with Raf-1 was detected by immunoprecipitation. Here, we demonstrated that apelin-13 increased the expression of 14-3-3, Raf-1 phosphorylation and ERK1/2 phosphorylation in a concentration- dependent and time-dependent manner at 0~4 μmol/L and 0~48 h. 14-3-3 inhibitor Difopein decreased the apelin-13-induced Raf-1 phosphorylation, ERK1/2 phosphorylation, expression of cyclinDl and cyclinE. Furthermore, apelin-13 promoted the combination of 14-3-3 protein and Raf-1, Difopein significantly inhibited the combination of 14-3-3 and Raf-1 stimulated by apelin-13. Similarly, Difopein significantly inhibited the VSMCs proliferation stimulated by apelin-13. Our results revealed that Raf-1 +14-3-3-ERK1/2 signaling cascades mediated the effect of apelin-13 on rat VSMCs proliferation.
期刊：
Journal of Applied Polymer Science,2011年122(5):3248-3254 ISSN：0021-8995
通讯作者：
Liu, Yanfei
作者机构：
[Liu, Yanfei; Huang, Kelong; Liu, Suqin] Cent South Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Peng, Dongming] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Liu, Zhenbao] Chinese Acad Med Sci, Inst Biomed Engn, Tianjin 300192, Peoples R China.;[Liu, Zhenbao] Peking Union Med Coll, Tianjin 300192, Peoples R China.
通讯机构：
[Liu, Yanfei] C;Cent South Univ, Sch Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
drug delivery systems;functionalization of polymers;microencapsulation;polycarbonates
摘要：
A novel biodegradable aliphatic polycarbonate, poly(propylene carbonate maleate) (PPCMA) was synthesized by terpolymerization of carbon dioxide, propylene oxide, and maleic anhydride (MA), using a polymer supported bimetallic complex as catalyst. The utility of PPCMA to encapsulate and control the release of drug pazufloxacin mesilate (PZFX), via microcapsules, was investigated. PPCMA microcapsules containing PZFX were elaborated by solvent evaporation method based on the formation of double W/O/W emulsion. The manufacturing parameters such as the volume ratio of V(PPCMA) : V(PZFX), the concentration of stabilizer gelatin in outer aqueous phase played major roles on microcapsule characters, and were altered to optimize the process parameters. The PPCMA-PZFX microcapsules were obtained with smooth and spherical surface under optimum condition, the mean diameter of microcapsules was similar to 2 mu m, and the drug loading and drug encapsulation efficiency of the microcapsules were 22.9 +/- 1.05% and 82.1 +/- 2.03%, respectively. PZFX released from PPCMA microcapsules was found to reach 89.8 +/- 2.89% after 36d in a pH 7.4 phosphate-buffered solution, and the release profile obeyed the Higuchi equation. The results suggest that the new polymer PPCMA provides an alternative to degradable matrix polymers for long-term sustained releasing drug delivery systems. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 122: 3248-3254, 2011
摘要：
This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong (DU 26), Neiguan (PC 6), Quchi (LI 11) and Zusanli (ST 36) in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β (Pol β)experiments, the Pol β activity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic endonuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints
摘要：
BACKGROUND: Numerous man-made pollutants activate the aryl hydrocarbon receptor (AhR) and are risk factors for type 2 diabetes. AhR signaling also affects molecular clock genes to influence glucose metabolism. OBJECTIVE: We investigated mechanisms by which AhR activation affects glucose metabolism. METHODS: Glucose tolerance, insulin resistance, and expression of peroxisome proliferator-activated receptor-alpha (PPAR-alpha) and genes affecting glucose metabolism or fatty acid oxidation and clock gene rhythms were investigated in wild-type (WT) and AhR-deficient [knockout (KO)] mice. AhR agonists and small interfering RNA (siRNA) were used to examine the effect of AhR on PPAR-alpha expression and glycolysis in the liver cell line Hepa-1c1c7 (c7) and its c12 and c4 derivatives. Brain, muscle ARNT-like protein 1 (Bmal1) siRNA and Ahr or Bmal1 expression plasmids were used to analyze the effect of BMAL1 on PPAR-alpha expression in c7 cells. RESULTS: KO mice displayed enhanced insulin sensitivity and improved glucose tolerance, accompanied by decreased PPAR-alpha and key gluconeogenic and fatty acid oxidation enzymes. AhR agonists increased PPAR-alpha expression in c7 cells. Both Ahr and Bmal1 siRNA reduced PPAR-alpha and metabolism genes. Moreover, rhythms of BMAL1 and blood glucose were altered in KO mice. CONCLUSIONS: These results indicate a link between AhR signaling, circadian rhythms, and glucose metabolism. Furthermore, hepatic activation of the PPAR-alpha pathway provides a mechanism underlying AhR-mediated insulin resistance.
摘要：
A further chemical investigation of the plant Rubus corchorifolius L. f., collected in Hunan Province, afforded two new ent-kauranoids 6 and 7. Their structures were elucidated by various spectroscopic methods.
