摘要：
Objective: To probe into the mechanisms of electroacupuncture (EA) at Shangjuxu (ST 37) for treatment of the ulcerative colitis (UC). Methods: Forty male Wistar rats were randomly divided into 4 groups: a normal group, a model group, a Shangjuxu group and a non-acupoint group, 10 rats in each group. The UC rat model was made with enema of trinitro-benzenesulfonic acid (TNBSA), and the changes of interleukin-1 beta (IL-1 beta) and interleukin-4 (IL-4) contents after EA at Shangjuxu (ST 37) were observed. Results: EA at Shangjuxu (ST 37) could significantly decrease the IL-1 beta content and increase the IL-4 content in the colic tissues of the UC rats with significant differences as compared with the model group and the non-acupoint group (P<0.05 or P<0.01). Conclusion: The mechanisms of EA at Shangjuxu (ST 37) for treatment of the UC rats is possibly related with the decrease of IL-1 beta, a inflammation-promoting cytokine, and the increase of IL-4, a anti-inflammatory cytokine.
作者机构：
[Cai Guangming; Shen Qian; Wu Na; Zhao Yanling; Wang Feng] Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Shen Qian; Wu Na; He Qun] Hunan Univ Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Wu Na] Inst Chinese Mat Med, 302 Hosp PLA, Beijing 100039, Peoples R China.
通讯机构：
[Wu Na] I;Inst Chinese Mat Med, 302 Hosp PLA, Beijing 100039, Peoples R China.
作者机构：
[Yang, Xu-Guang; Ma, Ting-Ting; Li, Ying; Liang, Fan-Rong; Yu, Shu-Yuan; Tian, Xiao-Ping] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.;[Sun, Guo-Lie] Hubei Coll Tradit Chinese Med, Wuhan 430065, Hubei, Peoples R China.;[Yan, Jie; Chang, Xiao-Rong] Hunan Univ Tradit Chinese Med, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Liang, Fan-Rong] C;Chengdu Univ Tradit Chinese Med, Chengdu 610075, Sichuan, Peoples R China.
关键词：
Nepean Dyspepsia Index;Functional dyspepsia;Health-related quality of life;Validation studies;Questionaires
摘要：
AIM: To assess the reliability and validity of the translated version of Nepean Dyspepsia Index (NDI) in Chinese patients with documented functional dyspepsia (FD).METHODS: The translation process included forward translation, back translation, pretest and cross-cultural adaptation. Reliability and validity of the translated version were examined by asking 300 subjects to complete the Chinese version of the NDI. The mean age of subjects was 39.24 years and 68.7% of the subjects were women. Internal consistency analysis with Cronbach's α was performed to test the reliability. Correlation analysis was used to assess the content validity. Factor analysis and structural equation models were used to assess the construct validity.RESULTS: The Cronbach's α coefficients ranged 0.833-0.960, well above the acceptable level of 0.70. Correlation analysis showed that each item had a strong correlation with the corresponding domain, but a weak correlation with other domains. Confirmatory factor analysis indicated that the comparative fit index was 0.94, higher than the acceptable level of 0.90.CONCLUSION: The Chinese version of the NDI is a reliable and valid scale for measuring health-related quality of life and disease severity in Chinese patients with FD.
作者机构：
[Wu Yan-shu; Zhang Qian; Jin Cheng; Xiao Xiao-he; Yan Dan] 302 Mil Hosp China, PLA Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Wu Yan-shu; Zhang Qian; Wang Jian] Chengdu Univ Tradit Chinese Med, Coll Pharm, Chengdu 611137, Peoples R China.;[Jiao Yi] Hunan Univ Tradit Chinese Med, Coll Pharm, Changsha 410002, Hunan, Peoples R China.;[Du Xiao-xi] State Food & Drug Adm, Ctr Drug Evaluat, Beijing 100038, Peoples R China.
通讯机构：
[Xiao Xiao-he] 3;302 Mil Hosp China, PLA Inst Chinese Mat Med, Beijing 100039, Peoples R China.
作者机构：
[Ren YongShen; Wang Jiabo; Zhao HaiPing; Yan Dan; Xiao XiaoHe; Zhou CanPing; Li HanBing; Zhao YanLing] 302 Mil Hosp, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.;[Ren YongShen; Zhao HaiPing; Li HanBing] Chengdu Univ Tradit Chinese Med, Chengdu 610075, Peoples R China.;[Zhou CanPing] Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Xiao XiaoHe] 3;302 Mil Hosp, China Mil Inst Chinese Mat Med, Beijing 100039, Peoples R China.
关键词：
Rhubarb;Strobal;Cold/Hot nature of Chinese medicines;cold/hot plate differentiating assay
摘要：
In this study, objective differences between the Cold (Han) and Hot (Re) nature of traditional Chinese medicines, e.g. Strobal and Rhubarb, are determined by using a cold/hot plate differentiation technology. A novel, self-designed cold/hot plate differentiating instrument, with methodological study, was used to investigate the intervention of Strobal and Rhubarb on the temperature tropism of mice. Compared with the ICR and BALB/c mice, it was found that KM mice on the cold/hot plate were more sensitive to the change of temperature, within the tolerant temperature range of 15-40 degrees C. The temperature tropism behavior of mice is influenced by treatment with Rhubarb and Strobal, as is the activity of ATPase in liver tissue. These trends are consistent with the definition of the Cold/Hot nature of Chinese medicines based on traditional Chinese medicinal theory. This study showed that the differences of the Cold/Hot nature of traditional Chinese medicines. might be objectively represented by the temperature tropism of animal by means of cold/hot differentiating assay.
作者机构：
[Zhang XueRu; Ren YongShen; Wang JiaBo; Zhao HaiPing; Xiao XiaoHe; Zhou CanPing; Zhao YanLing] 302 Mil Hosp China, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.;[Zhang XueRu; Xia XinHua; Zhou CanPing] Hunan Univ Chinese Med, Coll Pharmaceut Sci, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Xiao XiaoHe] 3;302 Mil Hosp China, China Mil Inst Chinese Meteria Med, Beijing 100039, Peoples R China.
关键词：
"Cold" and " Hot" natures of Chinese medicine;evaluation;temperature tropism;ethology;drug process;Coptis chinensis Franch
摘要：
The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of Chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40 A degrees C) significantly increased (P < 0.05), suggesting the enhancement of Hot tropism. The internal indexes of adenosine triphosphatase (ATPase) activity and oxygen consuming volume decreased significantly (P < 0.05), suggesting the decapability of energy metabolism. This external behavior of Hot tropism might reflect the internal Cold nature of C. chinensis Franch. However, the processed materials of C. chinensis Franch exhibited a different Cold nature in temperature tropism compared with crude C. chinensis Franch (CC): the Cold nature of bile-processed C. chinensis Franch (BC) enhanced while the ginger-processed C. chinensis Franch (GC) changed inversely. The changing sequence was consistent with the theoretical prognostication. It is indicated that the external Cold & Hot natures of Chinese medicine may possibly reflect in an ethological way for the changes of animal's temperature tropism which might be internally regulated by the body's energy metabolism.
期刊：
Journal of Zhejiang University. Science. B,2009年10(3):172-179 ISSN：1673-1581
通讯作者：
He, Ying-chun
作者机构：
[Tian, Dao-fa; He, Ying-chun] Chinese Med Univ Hunan, Fac Integrat Med, Changsha 410007, Peoples R China.;[Lu, Fang-guo] Chinese Med Univ Hunan, Fac Basic Med, Changsha 410007, Peoples R China.;[Tang, Fa-qing] Cent S Univ, Dept Clin Lab, Xiangya Hosp 1, Changsha 410008, Peoples R China.
通讯机构：
[He, Ying-chun] C;Chinese Med Univ Hunan, Fac Integrat Med, Changsha 410007, Peoples R China.
摘要：
OBJECTIVE: To investigate the enhancive effect of N,N'-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). METHODS: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C(57)BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C57BL/6J cancerous lesion-inducing group (CI), and C57BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. RESULTS: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). CONCLUSION: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.
作者：
Li Ying;Liang Fanrong;Yang Xuguang;Tian Xiaoping;Yan Jie;...
期刊：
HEADACHE,2009年49(6):805-816 ISSN：0017-8748
通讯作者：
Li, Y
作者机构：
[Li Ying; Liang Fanrong; Yang Xuguang; Tian Xiaoping; Tang Yong; Ma Tingting] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Yan Jie; Chang Xiaorong; Lan Lei; Yao Wen] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Sun Guojie; Zhou Li; Zou Ran] Hubei Coll Tradit Chinese Med, Wuhan, Hubei, Peoples R China.
通讯机构：
[Li, Y ] ;37 Shi Er Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.
关键词：
acupuncture therapy;acute migraine;acupuncture points;sham;acupuncture;traditional Chinese medicine
摘要：
Aim of the study: The inhibitive effect of BuYang HuanWu Decoction (BYHWD) and its major components on vascular intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix protein. Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, alkaloid, glycoside, BYHWD and atorvastatin groups. Rat aorta intima in all groups were injured by insesion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proteins in vascular cells associated with cell cycle including proliferating cell nuclear antigen(PCNA), cyclinD(1) and cyclinE, and extracellular matrix(ECM) proteins including collagen I (Col-1) and fibronectin (FN), further to discover the involved biologically active substances and the potential mechanisms. Results: Alkaloid and glycosid isolated from BYHWD were more effective than BYHVVD in the inhibition of intimal hyperplasia and the expressions of PCNA, cyclinD(1), cychnE, Col-I and FN, suggesting that alkaloid and glycoside may be the main components of BYHWD responsible for the observed inhibition of excessive hyperplasia of vascular intima. Conclusions: The mechanism associated with the anti-hyperplasia activity of BYHVVD and its effective components may be related to the blockage of cell cycles of VSMC, and the inhibition of the ECM protein synthesis, even the increased degradation of ECM proteins. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Zheng, Hui; Tian, Xiao-ping; Li, Ying; Liang, Fan-rong; Yu, Shu-guang; Liu, Xu-guang; Tang, Yong; Yang, Xu-guang; Ma, Ting-ting] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Yan, Jie; Chang, Xiao-rong; Yu, Shu-yuan] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Sun, Guo-jie] Hubei Coll Tradit Chinese Med, Wuhan, Hubei, Peoples R China.;[Zhang, Hong-xing] 1 People Hosp Wuhan City, Wuhan, Hubei, Peoples R China.
通讯机构：
[Liang, FR ] ;Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.
关键词：
Functional Dyspepsia;Acupuncture Treatment;Acupuncture Group;Specific Acupoint;Transcutaneous Electric Acupoint Stimulation
摘要：
Acupuncture is widely used in China to treat functional dyspepsia (FD). However, its effectiveness in the treatment of FD, and whether FD-specific acupoints exist, are controversial. So this study aims to determine if acupuncture is an effective treatment for FD and if acupoint specificity exists according to traditional acupuncture meridians and acupoint theories. This multicenter randomized controlled trial will include four acupoint treatment groups, one non-acupoint control group and one drug (positive control) group. The four acupoint treatment groups will focus on: (1) specific acupoints of the stomach meridian; (2) non-specific acupoints of the stomach meridian; (3) specific acupoints of alarm and transport points; and (4) acupoints of the gallbladder meridian. These four groups of acupoints are thought to differ in terms of clinical efficacy, according to traditional acupuncture meridians and acupoint theories. A total of 120 FD patients will be included in each group. Each patient will receive 20 sessions of acupuncture treatment over 4 weeks. The trial will be conducted in eight hospitals located in three centers of China. The primary outcomes in this trial will include differences in Nepean Dyspepsia Index scores and differences in the Symptom Index of Dyspepsia before randomization, 2 weeks and 4 weeks after randomization, and 1 month and 3 months after completing treatment. The important features of this trial include the randomization procedures (controlled by a central randomization system), a standardized protocol of acupuncture manipulation, and the fact that this is the first multicenter randomized trial of FD and acupuncture to be performed in China. The results of this trial will determine whether acupuncture is an effective treatment for FD and whether using different acupoints or different meridians leads to differences in clinical efficacy. Clinical Trials.gov Identifier: NCT00599677.
摘要：
This study was aimed at procuring directly and identifying the bacteria which had been found in paraffin-embedded liver tissues of hepatocellular carcinoma (HCC) patients. In our previous studies, Helicobacter spp. had been detected by polymerase chain reaction (PCR) and observed by histology in the liver tissues of HCC patients but had never been cultured successfully. To obtain and identify the uncultured bacteria, laser microdissection and pressure catapulting (LMPC) techniques were applied. Following microdissection from the liver tissue sections, these bacteria were examined by PCR using Helicobacter genus-specific 16S rRNA primers and sequence analysis. Amplified products of 16S rRNA were positive in all six microdissected samples with bacteria, and showed 99%-100% similarity with Helicobacter pylori by sequence analysis. Another H. pylori-specific 26 kDa gene (encoding one 26 kDa protein as H. pylori-specific antigen) was also tested by PCR. Four of six samples were positive. Therefore, Helicobacter spp. detected by PCR in the liver tissues of HCC patients in our previous studies are actually the bacteria observed by histology and identified as H. pylori by further sequence analysis. The laser-assisted microdissection technique can be extensively applied for identification of bacteria in tissue samples in bacteriology research.
摘要：
Ethnopharmacological relevance: Buyang Huanwu Decoction (BYHWD), a Chinese prescription that has been used for hundreds of years to treat paralysis, has gained attention recently due to its significant neuroprotective properties. Aim of the study: This study was to investigate whether BYHWD treatment protected axotomized rubrospinal neurons (RN) following spinal cord injury (SCI) in rats. Materials and methods: Adult rats received a right lateral funiculus transection at the level between C3 and C4, and were either treated with BYHWD or with distilled water (DW) via gastrogavage. Effects on RN viability and atrophy were determined by Nissl staining, axon regeneration was examined by biotinylated dextran amine (BDA) tracing techniques and functional recovery was studied by a test of forelimb usage during spontaneous vertical exploration. Results: RN cell number and mean somal size were 20% and 29% higher, respectively, in BYHWD-treated rats relative to DW-treated rats. There were a small number of BDA-labeled axons in the caudal of injury site in BYHWD-treated rats, whereas no caudal axonal regeneration was detected in DW-treated rats. BYHWD-treated rats used the injured forelimb more often than rats treated with DW. Conclusions: These results indicate that administration of BYHWD following SCI protects injured neurons, promotes regeneration, and enhances functional recovery. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Huang Guo-Dong] Nanchang Univ, Affiliated Hosp 1, Dept Integrat Med, Nanchang, Peoples R China.;[Li Jia-Bang] Cent S Univ, Xiangya Hosp, Changsha 410008, Peoples R China.;[Xiao Mei-Zhen; Huang Dao-Fu; Huang Yuan-Hua] Hunan Univ Chinese Med, Hengyang Hosp, Hunan, Peoples R China.;[Liu Juan] Changsha Med Coll, Changsha, Peoples R China.
通讯机构：
[Li Jia-Bang] C;Cent S Univ, Xiangya Hosp, Changsha 410008, Peoples R China.
关键词：
Gastritis;Helicobacter pylori;Platelet activating factor mastocarcinoma-related peptide;Volatile oil of amomum
摘要：
Objective： To observe the effect of volatile oil of amomum （VOA） on the expressions of mastocarcinoma-related peptide （PS2） and platelet activating factor （PAF） in helicobacter pylori- associated gastritis （HPG） and to analyze its potential mechanism. Methods： Eighty patients with HPG were randomly assigned to two groups, 42 patients in the treated group treated with 0.5 mL VOA, thrice per day; and the 38 patients in the control group receiving Western tertiary medicinal treatment. Gastroscopic picture and helicobacter pylori （HP） infection （by quick urease and Warthin- Starry stain） of the gastro-membrane, expressions of PS2 and PAF （by immunohistochemical assay and Western blotting） as well as the contents of aminohexose and phospholipid （by Neuhaus method） in the gastric membrane of all patients were detected before treatment and 4 weeks after treatment. The clinical efficacy in the two groups was compared. Results： The total effective rate in the treated group was 88.1%, which was significantly higher than that in the control group （78.9%, P〈0.05）. After treatment, in the treated group, gastric membranous contents of aminohexose and phospholipid was increased, expression of PS2 elevated but that of PAF lowered, all showing significant difference as compared with those in the control group （P〈0.01）. In the control group, the expressions of PS2 and PAF changed insignificantly. The radical eliminating rate of HP in the treated group and the control group was insignificantly different between them （76.1% vs. 65.8%, P 〉 0.05）. Conclusion： The mechanism of VOA for anti-gastritis might be related with its action in increasing the expression of PS2 and decreasing the expression of PAF, and thus regulating the hydrophobicity of the gastric membrane.
关键词：
Adult-onset type II citrullinemia (CTLN2);Aspartate-glutamate carrier (AGC);Citrin;Malate-aspartate shuttle;Neonatal intrahepatic cholestatic hepatitis (NICCD);Retrotransposal insertion;SLC25A13
摘要：
Deficiency of citrin, liver-type mitochondrial aspartate-glutamate carrier, is an autosomal recessive disorder caused by mutations of the SLC25A13 gene on chromosome 7q21.3 and has two phenotypes: neonatal intrahepatic cholestatic hepatitis (NICCD) and adult-onset type II citrullinemia (CTLN2). So far, we have described 19 SLC25A13 mutations. Here, we report 13 novel SLC25A13 mutations (one insertion, two deletion, three splice site, two nonsense, and five missense) in patients with citrin deficiency from Japan, Israel, UK, and Czech Republic. Only R360X was detected in both Japanese and Caucasian. IVS16ins3kb identified in a Japanese CTLN2 family seems to be a retrotransposal insertion, as the inserted sequence (2,667-nt) showed an antisense strand of processed complementary DNA (cDNA) from a gene on chromosome 6 (C6orf68), and the repetitive sequence (17-nt) derived from SLC25A13 was found at both ends of the insert. All together, 30 different mutations found in 334 Japanese, 47 Chinese, 11 Korean, four Vietnamese and seven non-East Asian families have been summarized. In Japan, IVS16ins3kb was relatively frequent in 22 families, in addition to known mutations IVS11 + 1G > A, 851del4, IVS13 + 1G > A, and S225X in 189, 173, 48 and 30 families, respectively; 851del4 and IVS16ins3kb were found in all East Asian patients tested, suggesting that these mutations may have occurred very early in some area of East Asia.
期刊：
JOURNAL OF PHYSICAL CHEMISTRY A,2008年112(2):305-311 ISSN：1089-5639
通讯作者：
Xiao, Xiaoming
作者机构：
[Rong, Chunying; Xiao, Xiaoming; Liu, Shubin; Huang, Ying] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.;[Huang, Ying] Hunan Univ Tradit Chinese Med, Sch Pharm, Changsha 410007, Hunan, Peoples R China.;[Zhong, Aiguo] Taizhou Coll, Dept Chem, Linhai 317000, Zhejiang, Peoples R China.;[Liu, Shubin] Univ N Carolina, Renaissance Comp Inst, Chapel Hill, NC 27599 USA.
通讯机构：
[Xiao, Xiaoming] H;Hunan Normal Univ, Coll Chem & Chem Engn, Changsha 410081, Hunan, Peoples R China.
摘要：
Porphyrin and pincer complexes are both important categories of compounds in biological and catalytic systems. The idea to combine them is computationally investigated in this work. By employment of density functional theory (DFT), conceptual DFT, and time-dependent DFT approaches, structure, spectroscopy, and reactivity properties of porphyrin pincers are systematically studied for a selection of divalent metal ions. We found that the porphyrin pincers are structurally and spectroscopically different from their precursors and are more reactive in electrophilic and nucleophilic reactions. A few quantitative linear/exponential relationships have been discovered between bonding interactions, charge distributions, and DFT chemical reactivity indices. These results are implicative in chemical modification of hemoproteins and understanding chemical reactivity in heme-containing and other biologically important complexes and cofactors.
摘要：
The series piezoelectric quartz crystal (SPQC) sensing technique is a rapid and sensitive method for detection of microorganisms. In the present study, the detection device was composed of detecting system, signal generating system and data analyzing system. To magnify the amount of detection samples, eight independent SPQC sensors were parallel connected to form a muti-channel detecting unit. Electrodes were separated from the SPQC sensor and immersed into culture medium to detect the change of solution conductivity. The cell constant k was determined as 0.05 m, and the sensitivity interval of the device was from 550 to 600 μs. To maintain sensitivity of the SPQC sensor, a novel culture medium amino acid broth (AaB) was developed. It was nutrient with low conductivity and satisfied our detection device. For determining frequency detection time (FDT) expediently and accurately, FDT was defined afresh with fitting–differentiating method. Pathogens Staphylococcus aureus and Shigella dysenteriae were determined with an automated detecting device and the methods mentioned above. The calibration curves of FDT against density of bacteria showed a linear correlation coefficient (R ≥ 0.99) over the range of 10–106 cells ml−1. Detection results all fell inside the 95% confidence interval of a standard pour plate counting method. The reproducibility was also reviewed, and results showed that the device was stable and sensitive even after 180 days of employment.
摘要：
A novel alkaloid, hupcrispatine (1), has been isolated from the unique Chinese species Huperzia crispate Ching. The structure of hupcrispatine has been elucidated as 9-amino-6-methyl-3-quinolone on the basis of spectral evidence.
