作者机构：
[Zhang, Leilei; Li, Peifeng; Li, Jitian; Cao, Xiangyang; Li, WY; Liu, Youwen; Li, Wuyin; Zhang, Ying] Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, Luoyang 471002, Henan, Peoples R China.;[Zhuang, Zhikun; Wang, Haibin; Zhang, Ying] Guangzhou Univ Chinese Med, Guangzhou 510405, Guangdong, Peoples R China.;[Jing, Zhenhao; Yuan, Qiang; Dong, Yipping] Henan Univ Chinese Med, Zhengzhou 450046, Henan, Peoples R China.;[Hong, Zhinan; Wei, Qiushi; He, Wei] Guangzhou Univ Chinese Med, Inst Orthopaed, Guangzhou 510240, Guangdong, Peoples R China.;[Hong, Zhinan; Wei, Qiushi; He, Wei] Guangzhou Univ Chinese Med, Affiliated Hosp 3, Guangzhou 510240, Guangdong, Peoples R China.
通讯机构：
[Li, WY ] L;[Wang, HB ] G;Luoyang Orthoped Traumatol Hosp, Orthoped Hosp Henan Prov, Med Ctr Hip, Luoyang 471002, Henan, Peoples R China.;Guangzhou Univ Chinese Med, Guangzhou 510405, Guangdong, Peoples R China.
关键词：
BMSC;OD;SOCS1;STAT1/3
摘要：
Our study showed that Signal transducer and activator of transcription (STAT)1 and STAT3 phosphorylation was firstly upregulated in the early stage of osteogenic differentiation (OD), and quickly eliminated in hours. Following with phosphorylation of STAT1/3, its downstream feedback regulator Suppressor of cytokine signaling 1 (SOCS1) protein also underwent a quick elevation. Further activation and deactivation of STAT1/3, by administrated with Colivelin and Nifuroxazide in Bone mesenchymal stem cells (BMSCs), increased and decreased SOCS1 expression, inhibited and promoted OD of BMSCs, respectively, as evidenced by Alizarin staining, alkaline phosphatase (ALP) activity, and determination of Run-related transcription factor 2 (RUNX2), Osteocalcin (OCN), ALP, and Bone sialoprotein (BSP). In addition, administration of Colivelin and Nifuroxazide caused and blocked inflammation and apoptosis of BMSCs. To further elucidate the role of STAT1/3-SOCS1 regulatory loop on OD of BMSCs, we overexpressed or silenced SOCS1 in BMSCs during OD. WB data showed that overexpression of SOCS1 repressed STAT1/3 phosphorylation, and knockdown of SOCS1 increased the phosphorylated STAT1/3. Further mechanism study showed that OD of BMSCs was elevated or reduced by SOCS1 overexpression or knockdown, respectively. The findings presenting indicated that the STAT1/3-SOCS1 axis may be exploited as an innovative strategy to enhance osteogenesis in regenerative medicine.
摘要：
Laparoscopic Nissen fundoplication and esophagoplasty are the standards for gastroesophageal reflux disease (GERD) and hiatal hernia (HH) repair. Biologically derived mesh is also associated with reduced recurrence. This study attempted to evaluate the effectiveness of a biological mesh in the 4K laparoscopic repair of HH. This retrospective study reviewed patients with a severe GERD complicated with HH from August 2019 to August 2020. All patients underwent the HH repair using a biological mesh under a 4K laparoscope accompanying Nissen fundoplication. Up to 16 months postoperatively, GERD-health-related quality-of-life (GERD-HRQL) scale, radiologic studies on HH recurrence, and symptoms were recorded. The mean surgical time and postoperative hospital stay were 70.9 +/- 8.72min, 4.8 +/- 0.76 days, respectively. The postoperative symptom relief rate was 96.5%, and no recurrence exhibited during follow-up. Dysphagia occurred in 10 (9.43%) patients. There were no intraoperative vagus nerve injury or postoperative complications, mesh infection, and reoperation for mesh. The tension-free repair of HH with the biological mesh is an option for clinical use, with effectiveness and few short-term complications being reported.
期刊：
Frontiers in Immunology,2022年13:2347 ISSN：1664-3224
通讯作者：
Yang, K.;Yang, T.;Chen, H.;Zeng, L.
作者机构：
[Zeng, Liuting; Chen, Hua] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Natl Clin Res Ctr Dermatol & Immunol Dis NCRC DID, Key Lab Rheumatol & Clin Immunol,Minist Educ,Dept, Beijing, Peoples R China.;[Li, Jun; Yang, Tiejun; Yu, Ganpeng] Peoples Hosp Ningxiang City, Dept Orthoped, Ningxiang City, Peoples R China.;[Yang, Kailin] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Xiang, Wang] First Peoples Hosp Changde City, Dept Rheumatol, Changde City, Peoples R China.
通讯机构：
[Zeng, L.; Chen, H.; Yang, T.] D;[Yang, K.] K;Department of Rheumatology and Clinical Immunology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases (NCRC-DID), Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing, China
关键词：
Curcumin;Curcuma longa extract;Rheumatoid arthritis;ankylosing spondylitis;Osteoarthritis;juvenile idiopathic arthritis;Systematic review;Meta-analysis
期刊：
Journal of Controlled Release,2022年348:42-56 ISSN：0168-3659
通讯作者：
Liu, Liang;Liu, Bin;Cai, Xiong
作者机构：
[Cai, Xiong; Hu, Shengtao; Su, Zhaoli; Hu, Mingyue; Wang, Wei; Yi, Ouyang; Wang, Shenzhi; Lin, Ye; Liao, Jin] Hunan Univ Chinese Med, Hosp & Inst Innovat & Appl Res Chinese Med 1, Dept Rheumatol, Changsha 410208, Hunan, Peoples R China.;[Liu, Bin; Yi, Ouyang] Hunan Univ, Coll Biol, Changsha 410082, Hunan, Peoples R China.;[Liu, Liang] Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Macau 999078, Peoples R China.
通讯机构：
[Cai, Xiong] D;[Liu, Liang] S;[Liu, Bin] C;State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. Electronic address:;College of Biology, Hunan University, Changsha, Hunan 410082, China. Electronic address:
关键词：
Hybrid membrane;Inflammatory cytokines;Prussian blue nanoparticle;Rheumatoid arthritis;Sinomenine hydrochloride
摘要：
Sinomenine is a bioactive alkaloid isolated from the Chinese medicinal plant of Sinomenium acutum (Thunb.) Rehd.et Wils. Currently, sinomenine hydrochloride (SIN) preparations, classified as a natural disease-modifying anti-rheumatic drug (nDMARD), have been used for therapy of rheumatoid arthritis (RA); however, the efficacy of SIN was seriously limited by its short half-life, low bioavailability, and dose-dependent adverse reactions. In this study, a biomimetic nanocomplex based on Prussian blue nanoparticles (PB NPs) was developed for overcoming clinical limitations of SIN and accordingly improving its efficacy. In vitro studies showed that the nanocomplexes significantly inhibited abnormal proliferation of fibroblast-like synoviocytes (FLSs) by scavenging reactive oxygen species (ROS) and inhibiting secretion of proinflammatory cytokines. In vivo imaging demonstrated that the improved immune-escape properties of the nanocomplexes resulted in markedly increased half-life of circulation and levels of accumulated drugs at arthritic sites of adjuvant-induced arthritis (AIA) rats. Notably, the nanocomplexes significantly suppressed joint inflammation and protected against bone destruction of AIA rats by inhibiting inflammatory cytokine secretion of the synovial macrophages and FLSs. These results indicate that the nanocomplexes provide an excellent carrier for controlled release and targeted accumulation of SIN within the arthritic sites, which consequently achieve disease-remitting effects of SIN on RA.
期刊：
Frontiers in Molecular Neuroscience,2022年14:785938 ISSN：1662-5099
通讯作者：
Wang, Yang
作者机构：
[Wang, Yang; Luo, Weikang; Yang, Zhaoyu] Cent South Univ, Xiangya Hosp, Inst Integrat Med, Dept Integrated Chinese & Western Med, Changsha, Peoples R China.;[Wang, Yang; Luo, Weikang; Yang, Zhaoyu] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China.;[Zhang, Wei] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Zhou, Dan] Hunan Univ Chinese Med, Period Off, Changsha, Peoples R China.;[Guo, Xiaohang] Hunan Univ Chinese Med, Med Sch, Changsha, Peoples R China.
通讯机构：
[Wang, Yang] C;Cent South Univ, Xiangya Hosp, Inst Integrat Med, Dept Integrated Chinese & Western Med, Changsha, Peoples R China.;Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Peoples R China.
作者机构：
[Peng, Shu-Wang] Hunan Univ Chinese Med, Hosp 1, Dept Gastrointestinal & Thyroid & Vasc Surg, Changsha, Peoples R China.;[Sheng, Jiang-Ming; Tian, Gui-Xiang; Liu, Ming-Hui; Xie, Hai-Qing] Cent South Univ, Xiangya Hosp 2, Dept Ultrasound, Changsha, Peoples R China.;[Li, Yan; Feng, Bai-Sui] Zhengzhou Univ, Affiliated Hosp 2, Dept Gastroenterol, Zhengzhou, Peoples R China.;[Peng, Ke-Ping] Hunan Univ Chinese Med, Hosp 1, Dept Otorhinolaryngol Head & Neck surg, Changsha, Peoples R China.;[Tian, Gui-Xiang] Cent South Univ, Xiangya Hosp 2, Res Ctr Ultrasonog, Changsha, Peoples R China.
通讯机构：
[Gui-Xiang Tian] D;Department of Ultrasound, The Second Xiangya Hospital of Central South University, Changsha, China<&wdkj&>Research Center of Ultrasonography, The Second Xiangya Hospital of Central South University, Changsha, China
摘要：
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples, which was positively correlated with UC clinical symptoms. HDM-specific eosinophils and Th2 cells were detected in CLF, which could be specifically activated by exposing to HDM in the culture. Direct exposure to HDM induced eosinophil activation in the colon of UC patients. UC patients displayed elevated levels of Th2 cytokines in the serum. UC clinical symptom scores were positively correlated with serum levels of Th2 cytokines. HDM was detected in UC patients' stools, which was positively correlated with UC clinical symptoms. Direct exposure to HDM could trigger eosinophilic activation of the colon.
期刊：
Complementary Therapies in Medicine,2022年67:102831 ISSN：0965-2299
作者机构：
[Zhao, Jie] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Hunan, Peoples R China.;[Ai, Jun] Guangxi Univ Chinese Med, Basic Med Sch, Nanning 530200, Guangxi, Peoples R China.;[Shi, Wei; Meng, LiFeng; Mo, Chao] Guangxi Univ Chinese Med, Affiliated Hosp 1, Dept Nephrol, 327 Xian Hu Rd, Nanning 530023, Guangxi, Peoples R China.;[Mo, Chao] Guangxi Univ Chinese Med, Grad Sch, Nanning 530200, Guangxi, Peoples R China.;[Meng, LiFeng] Southwest Med Univ, Tradit Chinese & Western Med Lab Controlling Orga, Luzhou 646000, Sichuan, Peoples R China.
摘要：
BACKGROUND: Bailing Capsule (BLC), Jinshuibao (JSB), Huangkui Capsule (HKC), Uremic Clearance Granule (UCG), Tripterygium glycosides (TG), Compound Xueshuantong Capsule (CXC), and Shenyan Kangfu Tablet (SYKFT) as classic Chinese patent medicines (CPMs), have been widely used and shown beneficial effects on the treatment of early diabetic kidney disease (DKD). However, the comparative efficacy of seven CPMs in the treatment of early DKD remains unknown. OBJECTIVE: To evaluate and compare the efficacy of seven CPMs (BLC, JSB, HKC, UCG, TG, CXC, SYKFT) combined with angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB) on early DKD by a Bayesian network meta-analysis (NMA) of randomized controlled trials (RCTs). METHODS: A comprehensive and systematic literature search was performed in PubMed, Embase, Cochrane Library, Web of Science, Clinical Trials.gov, China Biology Medicine, Chinese National Knowledge Infrastructure, Chinese Scientific Journal, and Wanfang databases from inception to March 14, 2021, for full-text RCTs that evaluated the efficacy of seven CPMs combined with ACEI/ARB on patients with early DKD. Two reviewers independently screened studies for eligibility, extracted data, and assessed the risk of bias. Agreement between reviewers was measured using kappa statistics. Mean difference (MD) and odds ratio (OR) were calculated to evaluate continuous variables and dichotomous, respectively. The random effect modeling NMA was performed and the ranking probability of interventions in various outcomes was also conducted based on the surface under the cumulative ranking curve (SUCRA). Begg's and Egger's tests were used to evaluate publication bias. The certainty of the evidence for outcomes was evaluated according to the GRADE system. RESULTS: A total of 62 RCTs with 5362 patients with early DKD were identified. The value of Kappa calculated for the various parameters extracted by the two investigators was 0.821 (P<0.001). Among these CPMs, UCG+ACEI/ARB showed the best effectiveness for urinary albumin excretion rate (UAER) (MD 32.25, 95% CrI 19.11-45.67, low certainty) with SUCRA 92%. JSB+ACEI/ARB showed the highest effectiveness for 24-h urinary total protein (24-h UTP) (MD 76.92, 95% CrI 53.54-100.58, low certainty) with SUCRA 97%. CXC+ACEI/ARB showed the highest effectiveness for serum creatinine (SCr) (MD 26.02, 95% CrI 6.10-45.95, low certainty) with SUCRA 96%. HKC+ACEI/ARB showed the highest effectiveness for blood urea nitrogen (BUN) (MD 1.46, 95% CrI 0.42-2.54, very low certainty) with SUCRA 86%. BLC+ACEI/ARB showed significant differences in triglyceride (TRIG) (MD -1.17, 95% CrI -1.93 to -0.43, low certainty) with SUCRA 90%, total cholesterol (TC) (MD -1.17, 95% CrI -1.97 to -0.39, very low certainty) with SUCRA 90%, and C-reaction protein (CRP) (MD -0.90, 95% CrI -1.51 to -0.32, very low certainty) with SUCRA 76%. CONCLUSIONS: CPMs+ACEI/ARB might be positive efficacious interventions from which patients with DKD will derive benefit. UCG+ACEI/ARB, JSB+ACEI/ARB, CXC+ACEI/ARB, and HKC+ACEI/ARB might be potentially the preferred intervention for reducing UAER, 24-h UTP, SCr, and BUN levels, respectively. BLC+ACEI/ARB has a better impact on lowing TRIG, TC, and CRP levels in patients with early DKD. However, more high-quality, large-scale, multi-center RCTs and stronger head-to-head trials are required to confirm these findings.
摘要：
BACKGROUND: Pulmonary hypertension (PH) is a progressive disorder lacking a validated and effective therapy which characterized by elevated pulmonary arterial pressure, vascular remodeling and eventual death. FDA approved sildenafil is being used as a first-line drug for PH, however, neither survival rates nor quality of life have been improved because of side effects and patient noncompliance. Thus, the exploration of novel therapeutic drugs is urgently needed. Astragaloside IV (ASIV) exhibits a protective effect on HPH, but its mechanisms of action is unclear. HYPOTHESIS: CD4+T cell subsets, Tfh and Tfr cells, may contribute to the development of chronic hypoxia-induced PH (HPH). We hypothesized that ASIV could effectively ameliorates pulmonary vascular remodeling of HPH by restraining the Tfh cell response and expanding Tfr cell response. METHODS AND RESULTS: HPH mice model was established by exposure to chronic hypoxia for 21 days. Mice were randomly assigned to six groups: NaCl group, model group, SN group (100mg/kg of sildenafil), low-dose group (20mg/kg of ASIV), medium-dose group (40mg/kg of ASIV) and high-dose group (80mg/kg of ASIV). Primary culture and identification of distal pulmonary artery smooth muscle cells (PASMCs) in mice were established. Here, we demonstrated that ASIV treatment could significantly ameliorate the increase of mean PAP, RV/ (LV+S) ratio and PAMT in HPH mice. ASIV inhibited Tfh cell differentiation and IL-21 production, but promoted Tfr cell differentiation and TGF-β, IL-10 production. Chronic hypoxia promoted germinal center B cell responses, which inhibited by ASIV. ASIV regulated Tfh and Tfr cell differentiation by inhibiting the phosphorylation of mTOR signaling pathway, and the effect of ASIV-H was better than that observed in the SN group. ASIV inhibited the proliferation, migration and adhesion of PASMCs in vitro. Moreover, ASIV significantly downregulated the protein level of RhoA and upregulated the protein level of p27 in PASMCs under hypoxic condition. CONCLUSION: Collectively, ASIV may regulate Tfh and Tfr cell responses to subsequently repress pulmonary vascular remodeling and hypoxic pulmonary hypertension.
作者机构：
[Zhu, Xu] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Dept Epidemiol & Hlth Stat, Changsha, Hunan, Peoples R China.;[Xue, Jing] Cent South Univ, Xiangya Hosp, Dept Sci Res, Changsha, Hunan, Peoples R China.;[Liu, Zheng] Shandong First Med Univ, Hosp Affiliated 1, Dept Anesthesiol, Shandong Prov Qianfoshan Hosp, Jinan, Shandong, Peoples R China.;[Xiang, Jingsha; Dai, Wenjie] Cent South Univ, Xiangya Sch Publ Hlth, Changsha, Hunan, Peoples R China.;[Zhou, Qiaoling; Xu, Hui; Chen, Wenhang] Cent South Univ, Xiangya Hosp, Dept Nephrol, Changsha, Hunan, Peoples R China.
摘要：
OBJECTIVE: The effect of the serum chloride (Cl) level on mortality in critically ill patients with acute kidney injury (AKI) remains unknown. We sought an association between mortality and serum Cl. METHODS: We identified AKI patients in the eICU Collaborative Research Database from 2014 to 2015 at 208 US hospitals. The outcomes included in-hospital and intensive care unit (ICU) mortality. Time-varying covariates Cox regression models and the Kaplan-Meier (K-M) curves were used to assess the association between serum Cl levels and mortality. Multivariable adjusted restricted cubic spline models were used to analyze the potential nonlinear relationship between mortality and serum Cl. RESULTS: In total, 4,234 AKI patients were included in the study. Compared with normochloremia (98≤chloride<108mEq/L), hypochloremia (Cl<98mEq/L) was associated with mortality (adjusted hazard ratio [HR] for in-hospital mortality 1.46, 95% confidence interval [CI] 1.20-1.80, P = 0.0003; adjusted HR for ICU mortality 1.37, 95% CI 1.05-1.80, P = 0.0187). Hyperchloremia showed no significant difference in mortality compared to normochloremia (adjusted HR for in-hospital mortality 0.89, 95% CI 0.76-1.04, P = 0.1438; adjusted HR for ICU mortality 0.87, 95% CI 0.72-1.06, P = 0.1712). Smoothing curves revealed continuous non-linear associations between serum Cl levels and mortality. The K-M curve showed that patients with hypochloremia presented with a lower survival rate. CONCLUSIONS: Lower serum Cl levels after ICU admission was associated with increased in-hospital and ICU mortality in critically ill patients with AKI. The results should be verified in well-designed prospective studies.
作者机构：
[Yuan, Xin; Wang, Tuanmei; Wang, Guohong; Yan, Shuyuan; Wang, Yanling; He, Jun; Peng, Xiangwen; Wang, Dongbo] Hunan Normal Univ, Changsha Hosp Maternal & Child Hlth Care, Changsha 410000, Peoples R China.;[Yuan, Xin] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Peng, Xiangwen] H;Hunan Normal Univ, Changsha Hosp Maternal & Child Hlth Care, Changsha 410000, Peoples R China.
关键词：
ASXL1;IVF;whole-exome sequencing
摘要：
RATIONALE: Bohring-Opitz syndrome is a severe congenital disorder associated with a de novo mutation in the additional sex combs-like 1 (ASXL1) gene, and it is characterized by symptoms that include developmental delay and musculoskeletal and neurological features. PATIENT CONCERNS: The patient was a girl, an in vitro fertilization (IVF) baby, with delayed motor development, drooling, short stature, slow growth, low muscle tone, image diagnosis of hypoplasia of the corpus callosum, delayed tooth eruption, high palatal arch, adduction of the thumb, drooling, not chewing, excessive joint activity, and ligament relaxation. DIAGNOSIS: Whole-exome sequencing analysis detected 1 novel disruptive frameshift mutation in ASXL1 in the proband but wild-type ASXL1 in both parents. INTERVENTIONS: Approximately 1 year of rehabilitation training, which included exercise therapy, toy imitation operation, cognition of daily objects, daily living skills training, gesture language training, oral muscle training, and hand movement training. OUTCOMES: After approximately 1 year of training, the patient was 3 years old and able to eat normally without drooling. She was able to grasp objects and pick them up after they fell. She was able to grasp small objects and actively played with toys. In addition, she was able to crawl on the floor (at slow speed, with poor initiative), stand with assistance, and walk with assistance; she was unstable when standing unassisted (standing unassisted for 8 seconds at most during training). LESSON: ASXL1 c.3762delT is a novel mutation that may be caused by IVF. This finding suggests that appropriate gene mutation detection approaches may be necessary for IVF technology.
作者：
Su, Yuting;Peng, Yao;Ren, Jie;Wu, Shangjie;Lei, Si;...
期刊：
Journal of Analytical Methods in Chemistry,2022年2022 ISSN：2090-8865
作者机构：
[Peng, Yao; Ren, Jie; Su, Yuting; Li, Juan; Li, Shunxiang] Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha 410208, Peoples R China.;[Peng, Yao; Ren, Jie; Su, Yuting; Li, Juan; Li, Shunxiang] Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha 410208, Peoples R China.;[Wu, Shangjie; Lei, Si; Peng, Fei] Cent South Univ, XiangYa Hosp 2, Changsha 410008, Peoples R China.;[Sun, Zhina; He, Xiuqing] Qinghai Red Cross Hosp, Xining 810099, Peoples R China.
摘要：
Berberidis Cortex is rich in alkaloids, and many of them have antibacterial, anti-inflammatory, and hypoglycemic activities. However, few research studies have focused on the quantitative analysis of multiple components from Berberidis Cortex. In this study, a new quality evaluation strategy for Berberidis Cortex was developed and validated by high-performance liquid chromatography (HPLC), which involved single marker, fingerprint, and stoichiometric methods. Using berberine hydrochloride as an internal reference, the relative correction factors of palmatine hydrochloride, magnoline, and jatrorrhizine hydrochloride were 2.4537, 0.9783, and 1.0035, respectively, and their durabilities were also well performed. In addition, both methods mentioned above were used to compare the mass fractions of four isoquinoline alkaloids in ten batches of Berberidis Cortex from different origins. These results indicated that the approach applied in this study was accurate and feasible. The fingerprints of these ten batches of Berberidis Cortex were established, and eleven components were identified with the similarity greater than 0.993. Both cluster and principal component analysis were carried out based on the peak area of these components, the results demonstrated that these ten batches of Berberidis Cortex were divided into two groups and the distribution of the medicinal material was basically consistent. Therefore, quantitative analysis of multicomponents by single marker (QAMS) can be widely used in the quality control of Berberidis Cortex as theoretical basis.
期刊：
Journal of Ethnopharmacology,2022年295:115357 ISSN：0378-8741
通讯作者：
Ge, J.
作者机构：
[Deng, Xianguang; Liu, Lifang; Zhao, Da; Ge, Anqi; Ge, Jinwen] First Hosp Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Yang, Kailin; Ge, Jinwen] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Hunan, Peoples R China.;[Ge, Jinwen] Hunan Acad Chinese Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Anqi Ge; Lifang Liu] T;[Kailin Yang; Jinwen Ge] K;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Hunan Academy of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, China
关键词：
Breast cancer;Meta-analysis;Randomized controlled trial;Systematic review;Xihuang capsule;Xihuang pill
期刊：
Frontiers in Pharmacology,2022年13:2067 ISSN：1663-9812
通讯作者：
Fu, W.
作者机构：
[You, Xujun] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.;[Li, Qixin; You, Xujun; Fu, Wei] Guangzhou Univ Chinese Med, Shenzhen Baoan Tradit Chinese Med Hosp, Dept Androl, Shenzhen, Peoples R China.;[Wu, Yongrong] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Sheng, Wen] Hunan Univ Chinese Med, Androl Lab, Changsha, Peoples R China.;[Zhou, Qing] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Androl, Changsha, Peoples R China.
通讯机构：
Department of Andrology, Shenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China
关键词：
Astragalus-Scorpio;prostate cancer;PI3K;AKT;Astragaloside IV;polypeptide extract from scorpion venom;autophagy
摘要：
Superb microvascular imaging (SMI) is among the latest doppler ultrasound methods. It uses an advanced clutter filter to eliminate artifacts caused by breathing, movement and retains the low-speed blood signals in microvessels. The great advantage of SMI is that it can intuitively detect very slow blood signals in microvessels, providing clinicians with more significant information about flow distribution in the target area. Therefore, it is speculated that SMI has important application value. The purpose of this article is to outline the application of SMI in different parts of the body.
摘要：
In recent years, manganese dioxide (MnO2) nanoparticles with unique physicochemical properties have been widely used in many biomedical fields, such as biosensors, contrast agents, tumor therapy, and drug delivery. From these applications, MnO2 nanoparticles have great clinical translation potential. However, by contrast, the in vitro and in vivo biosafety of MnO2 nanoparticles have been deeply and thoroughly clarified for the clinical translation, which hinders their clinical applications. In this work, we deeply investigated the blood safety of MnO2 nanoparticles by conducting a series of in vitro and in vivo experiments. These included the effects of MnO2 nanoparticles on morphology of red blood cells, activation of platelets, coagulation functions, and toxicity of key organs. The obtained results show that these effects displayed a concentration-dependent manner of MnO2 nanoparticles. Different safe concentration ranges could be found in the different experimental index. This study provides important guidance for the specific biomedical applications of MnO2 nanoparticles, greatly accelerating their laboratory development and clinical translation.
摘要：
Lilium, a perennial crop with great ornamental, medicinal and edible value, has been frequently used as functional food and medicine. Lilium lancifolium Thunb. (L. lancifolium) and Lilium brownii F.E.Brown var.viridulum Baker (L. brownii) are the most used medicinal species in China. However, the flavor compounds of these two species have not yet been clear. Here, metabolomics and transcriptome analysis were used to reveal the difference of the bitter substances of L. lancifolium and L. brownii. Qualitative results indicated that nine compounds are commonly existed in L. lancifolium and L. brownii, while nine compounds are unique in L. lancifolium and eight compounds are unique in L. brownii. Furthermore, quantitative results revealed that the content of regaloside A in L. lancifolium was nearly 2-7 folds higher than that of L. brownii, and the content of regaloside B in L. lancifolium was about 4-16 folds higher than that of L. brownii. Regaloside C and E were not detected in L. brownii. Transcriptome analysis showed that there were 90 unique genes up-regulated in L. lancifolium samples in the pathway of phenylpropanoid biosynthesis and 75 unique genes up-regulated in L. brownii samples, which could be related to the different content and chemical structure specificity of phenylpropanoid glycerol glucosides in L. lancifolium and L. brownii. The results of our in-deep research provide new insights into the bitter substances of L. lancifolium and L. brownii, and a further consideration for the chemical consistency and quality evaluation for Lilii bulbus.
期刊：
Journal of Healthcare Engineering,2022年2022 ISSN：2040-2295
作者机构：
[Chen, Xi; Gong, Juan; Tang, Xueyong; Jiang, Yourang; Qi, Dongwei] Chongqing Hosp Tradit Chinese Med, Dept Dermatol, Chongqing 400021, Peoples R China.;[Li, Xin] Hunan Univ Chinese Med, Changsha 410208, Peoples R China.
摘要：
Traditional Chinese medicine classifies psoriasis (Ps) according to clinical manifestations, and its different clinical manifestations imply the pathogenesis and material evolution basis of Ps, especially biomarkers that are meaningful to identification of Ps, treatment response, and elucidation of the pathogenesis of the disease. This study aims to screen differential metabolites in plasma of psoriasis vulgaris (PV) of blood heat syndrome based on a widely targeted metabolomic technique and to analyze syndrome metabolic markers and metabolic pathways. Forty-five PV patients were recruited, including 21 cases of the blood heat syndrome group (BH-PPG), 24 cases of the non-blood-heat syndrome group (NBH-PPG), and 30 healthy cases of the normal control group (NPG). The UPLC-MS/MS detection platform, a self-developed database, and multivariate statistical analysis were applied to investigate the plasma metabolic differences. The biomarkers related to blood heat syndrome were screened using the principal component analysis method. A total of 479 metabolites were detected in the three groups of plasma samples; 72 different metabolites were sorted out in the BH-PPG/NPG group, 82 in the NBH-PPG/NPG group, and 8 in the BH-PPG/NBH-PPG group. Differential metabolites mainly consist of metabolites of organic acids, amino acids, carbohydrates, and nucleotides. Multiple metabolites ginkgolic acid, pyrroloquinoline quinone, L-aspartic acid, and citramalic acid were expected to be the potential biomarkers of blood heat syndrome PV. The formation and evolution processes may be associated with disorders and regulation of metabolic pathways, ferroptosis, carbon metabolism, and purine metabolism.
期刊：
Evidence-Based Complementary and Alternative Medicine,2022年2022 ISSN：1741-427X
通讯作者：
Li, J.
作者机构：
[Zhang, Zexin] Guangzhou Univ Chinese Med, Clin Med Coll 1, Guangzhou 510405, Peoples R China.;[Liu, Zifeng; Lin, Siqi] Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou 510405, Peoples R China.;[Han, Jun] Beijing Tcmages Pharmaceut Co Ltd, Natl & Local Joint Engn Res Ctr Key Technol Chine, Beijing 101301, Peoples R China.;[Yu, Yi; Li, Jing] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410000, Peoples R China.
通讯机构：
The First Affiliated Hospital Of Hunan University Of Chinese Medicine, Changsha, China
摘要：
<i>Background</i>. Tao-He-Cheng-Qi Formula (THCQF) is a traditional Chinese medicine that has been proven to have antitumor effects. The aim of this study was to elucidate the molecular targets and mechanisms of THCQF against colon cancer and construct a prognostic model based on network pharmacology, bioinformatics analysis, and in vitro experiments. <i>Methods</i>. Potential THCQF compounds and targets were retrieved from the Traditional Chinese Medicine Systems Pharmacology and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine databases. Differentially expressed genes for colon cancer were screened in The Cancer Genome Atlas and Gene Expression Omnibus databases. The anticolon cancer mechanisms of THCQF were explored using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Molecular docking simulations and molecular dynamics analysis were used to evaluate the binding between target proteins and active compounds. Finally, the identified compounds were used to treat colon cancer cells from the HCT116 cell line, and expression of mRNA and protein after relevant posttreatment were tested using real-time polymerase chain reaction and western blotting. <i>Results</i>. A total of 27 anticolon cancer targets of THCQF were selected, among which four genes (<i>CCNB1</i>, <i>CCNA2</i>, <i>IL1A</i>, and <i>MMP3</i>) were shown to effectively predict patient outcomes in a prognostic colon cancer model. GO and KEGG enrichment analyses indicated that the activity against colon cancer of THCQF was associated with the interleukin (IL)-4 and IL-3 signaling pathways. Two compounds in THCQF, aloe emodin (AE) and quercetin (QR), were shown to efficiently bind to cyclin B1, the protein encoded by <i>CCNB1</i>. Finally, incubation of HCT116 cells with AE and QR significantly decreased <i>CCNB1</i> mRNA expression and cyclin B1 levels. <i>Conclusions</i>. Taken together, the results indicate that AE and QR are the pivotal active compounds of THCQF, and <i>CCNB1</i> is the main molecular target through which THCQF exerts its anticolon cancer effects. The study findings provide insight for studies investigating the anticancer effects of other traditional Chinese medicines.
