摘要：
Background: Dental caries is the most prevalent bacterial biofilm-induced disease. Current clinical prevention and treatment agents often suffer from adverse effects on oral microbiota diversity and normal tissues, predominately arising from the poor biofilm-targeting property of the agents. Methods: To address this concern, we herein report dual-sensitive antibacterial peptide nanoparticles pHly-1 NPs upon acid and lipid-binding for treatment of dental caries. Amino acid substitutions were performed to design the peptide pHly-1. The potential, morphology and secondary structure of pHly-1 were characterized to elucidate the mechanisms of its pH and lipid sensitivity. Bacterial membrane integrity assay and RNA-seq were applied to uncover the antimicrobial mechanism of peptides under acidic condition. The in vitro and ex vivo antibiofilm assays were used to determine the antibiofilm performance of pHly-1 NPs. We also carried out the in vivo anti-caries treatment by pHly-1 NPs on dental caries animal model. Oral microbiome and histopathological analyses were performed to assess the in vivo safety of pHly-1 NPs. Results: The pHly-1 peptide underwent the coil-helix conformational transition upon binding to bacterial membranes in the acidic cariogenic biofilm microenvironment, thereby killing cariogenic bacteria. Under normal physiological conditions, pHly-1 adopted a ??-sheet conformation and formed nanofibers, resulting in negligible cytotoxicity towards oral microbes. However, in acidic solution, pHly-1 NPs displayed reliable antibacterial activity against Streptococcus mutans, including standard and clinically isolated strains, mainly via cell membrane disruption, and also suppressed in vitro and human-derived ex vivo biofilm development. Compared to the clinical agent chlorhexidine, in vivo topical treatment with pHly-1 NPs showed an advanced effect on inhibiting rat dental caries development without adverse effects on oral microbiota diversity and normal oral or gastric tissues. Conclusion: Our results demonstrated the high efficacy of dual-sensitive antimicrobial peptides for the selective damage of bacterial biofilms, providing an efficient strategy for preventing and treating dental caries.
摘要：
To explore the mechanism of Epimedii Folium (HF) and Notoginseng Radix (NR) intervention in vascular dementia (VD). This study used the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database to collect the active ingredients and potential drug targets of HF and NR, the Uniprot database to convert drug target names into gene names, GeneCards, Drugbank, Therapeutic Target Database, and Online Mendelian Inheritance in Man database to collect the potential disease targets of VD, and then combined them with the drug targets to construct the HF-NR-VD protein-protein interaction (PPI) network by Search Tool for the Retrieval of Interacting (STRING). Cytoscape (version 3.7.1) was used to perform cluster analysis of the PPI network. Metascape database was used for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The potential interaction of the main components of the HF-NR couplet medicine with core disease targets was revealed by molecular docking simulations. There were 23 predicted active ingredients in HF and NR, and 109 common drug targets that may be involved in the treatment of VD. Through PPI network analysis, 30 proteins were identified as core proteins owing to their topological importance. GO functional analysis revealed that the primary biological processes were mainly related to inflammation, apoptosis, and the response to oxidative stress. KEGG pathway enrichment analysis revealed that TNF and PI3K/Akt signaling pathways may occupy the core status in the anti-VD system. Molecular docking results confirmed that the core targets of VD had a high affinity for the main compounds of the HF-NR couplet medicine. We demonstrated the multi-component, multi-target, and multi-pathway characteristics of HF-NR couplet medicine for the treatment of VD and provided a foundation for further clinical application and experimental research.
通讯机构：
[Zhang, GM ; Liu, HP] H;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
Objective. To explore the molecular network mechanism of modified Taohong Siwu Decoction (MTHSWD) to interfere with premature ovarian failure based on systematic pharmacological strategy. Methods. The network pharmacology strategy was used to explore the potential mechanism of MTHSWD intervention in POF, and then it was verified through animal experiments. Mouse zona pellucida 3 was used as an antigen to subcutaneously immunize BALB/c female mice to establish an immune POF model. Mice were divided into MTHSWD low-, medium-, and high-dose groups, positive control group, model group, and normal group. After 30 days of drug intervention, ovarian tissue was taken for pathological hematoxylin-eosin (HE) staining, and immunohistochemical methods were used to detect the expression of TGF-beta 1 and TGF-beta RII and Smad2/3 protein expression in follicular wall granular cells and ovarian tissue, respectively. Results. Network pharmacology studies have shown that MTHSWD may interfere with the TGF-beta signaling pathway. Animal experimental research shows that, compared with the model group, the number of ovarian mature follicles in the MTHSWD groups and the positive group was significantly increased, and the number of atresia follicles decreased. Immunohistochemistry showed that, compared with the control group, the expression of TGF-beta 1, TGF-beta RII, and Smad2/3 in the follicular wall granulosa cells and ovarian tissues of MTHSWD groups was significantly higher than that of the model group (P < 0.05). Conclusion. MTHSWD may improve the ovarian function of POF mice by upregulating the protein expression of granulosa cells TGF-beta 1, TGF-beta RII, and Smad2/3.
作者机构：
[Wang, Wei; Zhong, Rui] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp,Xiangya Sch Med, Dept Med Oncol,Digest & Urinary Unit, Changsha 410013, Hunan, Peoples R China.;[Zhang, Qiong; Yi, Feng; Xiang, Fen] Yueyang Cent Hosp, Dept Emergency, Yueyang 414100, Hunan, Peoples R China.;[Qiu, Yan-Fang] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp,Xiangya Sch Med, Dept Radiat Therapy Oncol, Changsha 410013, Hunan, Peoples R China.;[Zhu, Lei] Hunan Univ Chinese Med, Sch Nursing, Changsha 410208, Hunan, Peoples R China.;[Zou, Yan-Hui] Cent South Univ, Hunan Canc Hosp, Affiliated Canc Hosp,Xiangya Sch Med, Hlth Serv Ctr, Changsha 410013, Hunan, Peoples R China.
通讯机构：
[Zhang, Qiong] D;Department of Emergency, Yueyang Central Hospital, Yueyang 414100, Hunan Province, China.
关键词：
Acute hepatitis of unknown etiology;Adenovirus;Coronavirus disease;Immune system;Multi-system inflammatory syndrome in children;Vaccine
摘要：
Two years after the coronavirus disease 2019 (COVID-19) pandemic, acute hepatitis of unknown etiology in children (AHUCD) began to be reported worldwide. The novel coronavirus and adenovirus were found in pathogen and antibody tests in AHUCD cases reported by the World Health Organization. Children are not exposed to the viruses that children are generally exposed to owing to COVID-19 infection preventive measures such as isolation and wearing masks; therefore, some researchers have speculated that this disease is related to reduced exposure to pathogens. Some scientists have also speculated that the disease is related to liver injury and adenoviral hepatitis, which are the sequelae of COVID-19. Some evidence also suggests a weak association between the disease and COVID-19 vaccination. Therefore, further research and investigation of the pathogenesis, preventive measures, and early treatment of hepatitis of unknown etiology are required. This study aimed to synthesize available evidence to further elucidate this disease in order to treat and prevent it effectively.
摘要：
Reconfigurable wireless network can flexibly provide efficient spectrum access service and keep stable operation in highly dynamic environment. In this paper, a primary-prioritized recurrent deep reinforcement learning algorithm for dynamic spectrum access based on cognitive radio (CR) technology is proposed. The spectrum Markov state is modeled to capture the evolution behavior to achieve the priority queuing of the primary users and the secondary users. According to the spectrum access strategies of the secondary users under different optimal criteria, we can obtain the best tradeoff benefits of spectrum access fairness and throughput. Furthermore, we proposed a learning-based algorithm for dynamic spectrum access, which allows the secondary users to modify their parameters to select the optimal access policy to maximize network throughput utilization. The Dueling Deep
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-Network (Dueling DQN) with prioritized experience replay combined with recurrent neural network is used to improve the convergence speed. Extensive experimental results demonstrate that the proposed RDRL scheme outperforms the existing Dueling DQN and DQN schemes in terms of convergence speed and channel throughput.
作者机构：
[Liu, Lingzhi; Li, Yuejun; Tang, Ping; Wang, Hong; He, Haihui] Hunan Univ Chinese Med, Affiliated Hosp 3, Dept Oncol, Zhuzhou City, Peoples R China.;[Liu, Lingzhi; Li, Yuejun; Tang, Ping; Wang, Hong; He, Haihui] Hunan Coll Tradit Chinese Med, Affiliated Hosp 1, Dept Oncol, Zhuzhou City, Peoples R China.;[Peng, Qinghua; Liao, Linli] Hunan Univ Chinese Med, Hunan Prov Key Lab Prevent & Treatment Ophthalmol, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.;[Yan, Junfeng] Hunan Univ Chinese Med, Sch Informat, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yuejun Li] D;[Qinghua Peng] H;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha City, P. R. China<&wdkj&>Department of Oncology, The Third Affiliated Hospital of Hunan University of Chinese Medicine, Zhuzhou City, P. R. China<&wdkj&>Department of Oncology, The First Affiliated Hospital of Hunan College of Traditional Chinese Medicine, Zhuzhou City, P. R. China
关键词：
network pharmacology;gastric cancer;Sijunzi decoction;traditional Chinese medicine;bioactivity
摘要：
Objective:
Sijunzi decoction (SJZD) has been used for alleviating peptic ulcer or gastric discomfort, and treating spleen disorders since the Song Dynasty, but its pharmacological effect on human gastric cancer (GC) is still unclear. In this research, a network pharmacology-based strategy was applied to explore active ingredients, potential targets, and molecular mechanisms of SJZD against GC.
Methods:
The active compounds and potential targets of SJZD, as well as GC-associated gene targets, were retrieved from publicly available databases. Bioinformatics approaches were used to assess the network interaction, functional regulation, and signaling pathways between SJZD ingredients and GC targets. The anticancer effects of SJZD against GC were verified in vivo by a mouse subcutaneous model.
Results:
The results of network analysis showed that quercetin was the most active ingredient in SJZD. Several prominent target genes of SJZD were identified, such as AKT1 and STAT3. Gene ontology analysis revealed that the core anti-GC targets of SJZD included transcription factor activity and kinase activity. Pathway enrichment analysis indicated that GC patients could be benefited from SJZD treatment via modulation of signaling pathways related to endocrine system, cancer, and infectious disease. Furthermore, in vivo experiments showed that high-dose SJZD could inhibit GC xenograft tumor growth, reduce GC cell proliferation, induce GC cell apoptosis, and decrease the expression of p-AKT1 and p-STAT3.
Conclusions:
Taken together, our results suggest that SJZD can serve as an effective adjuvant therapeutic agent for GC patients.
摘要：
BackgroundStomach adenocarcinoma (STAD) arises from the mutations of stomach cells and has poor overall survival. Chemotherapy is commonly indicated for patients with stomach cancer following surgical resection. The most prevalent alteration that affects cancer growth is N6-methyladenosine methylation (m6A), although the possible function of m6A in STAD prognosis is not recognized. MethodThe research measured predictive FRGs in BLCA samples from the TCGA and GEO datasets. Data on the stemness indices (mRNAsi), gene mutations, copy number variations (CNV), tumor mutation burden (TMB), and corresponding clinical characteristics were obtained from TCGA and GEO. STAD from TCGA and GEO at 24 m6A was investigated. Lasso regression was used to construct the prediction model to assess the m6A prognostic signals in STAD. In addition, the correlation between m6a and immune infiltration in STAD patients was discussed using GSVA and ssGSEA analysis. Based on these genes, GO and KEGG analyses were performed to identify key biological functions and key pathways. ResultA significant relationship was discovered between numerous m6A clusters and the tumor immune microenvironment, as well as three m6A alteration patterns with different clinical outcomes. Furthermore, GSVA and ssGSEA showed that m6A clusters were significantly associated with immune infiltration in the STAD. The low-m6Ascore group had a lower immunotherapeutic response than the high-m6Ascore group. ICIs therapy was more effective in the group with a higher m6Ascore. Three writers (VIRMA, ZC3H13, and METTL3) showed significantly lower expression, whereas five authors (METTL14, METTL16, WTAP, RBM15, and RBM15B) showed considerably higher expression. Three readers (YTHDC2, YTHDF2, and LRPPRC) had higher levels of expression, whereas eleven readers (YTHDC1, YTHDF1, YTHDF3, HNRNPC, FMR1, HNRNPA2B1, IGFBP1, IGFBP2, IGFBP3, and RBMX) had lower levels. As can be observed, the various types of m6 encoders have varied ramifications for STAD control. ConclusionSTAD occurrence and progression are linked to m6A-genes. Corresponding prognostic models help forecast the prognosis of STAD patients. m6A-genes and associated immune cell infiltration in the tumor microenvironment (TME) may serve as potential therapeutic targets in STAD, which requires further trials. In addition, the m6a-related gene signature offers a viable alternative to predict bladder cancer, and these m6A-genes show a prospective research area for STAD targeted treatment in the future.
期刊：
Transactions on Emerging Telecommunications Technologies,2022年33(5) ISSN：2161-3915
通讯作者：
Ming, Zhao
作者机构：
[Li, Ting; Ming, Zhao] Cent South Univ, Sch Comp Sci & Engn, Changsha, Peoples R China.;[Liu, Wei] Hunan Univ Chinese Med, Sch Informat, Changsha, Peoples R China.;[Wang, Tian] Huaqiao Univ, Coll Comp Sci & Technol, Xiamen, Peoples R China.;[Li, Xiong] Hunan Univ Sci & Technol, Sch Comp Sci & Engn, Xiangtan, Peoples R China.;[Ma, Ming] SUNY Stony Brook, Dept Comp Sci, Stony Brook, NY 11794 USA.
通讯机构：
[Ming, Zhao] C;Cent South Univ, Sch Comp Sci & Engn, Changsha, Peoples R China.
摘要：
Due to their mobile character, ground vehicles and unmanned aerial vehicles (UAVs) are currently being considered as sensing devices that can collect data in the Internet of Things (IoT). Building and enhancing trust and security environments in data collection processes are fundamental and essential requirements. Here, we proposed a novel scheme named “Trust Data Collections via Vehicles joint with UAVs in the Smart Internet of Things” (T-SIoTs scheme), which targets to establish a trust-based environment for data collections by utilizing both trust vehicles and UAVs. First, to optimize security aspect, data center (DC) selected trust-based vehicles as mobile data collectors via analyzing and digging historical datasets. To promise coverage regions of data collections, several static stations are established, which can be utilized as static data collectors. Second, UAVs are arranged by the DC to collect data stored by both trust-based vehicles and static data collectors. In the T-SIoTs scheme, trajectories of UAVs are designed according to shortest-distance-first routing scheme. Comprehensive theoretical analyses and experiments have been provided to evaluate and support the T-SIoTs scheme. Compared with the previous studies, the T-SIoTs scheme can improve the security ratio by 46.133% to 54.60% approximately. And with the routing scheme, the energy consumptions of UAVs can be reduced by 46.93% approximately.
作者机构：
[Hu, Guangwan; Fan, Minxia; Guo, Mingquan; Li, Jing] Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China.;[Hu, Guangwan; Guo, Mingquan; Li, Jing] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China.;[Wang, Zhi] Hunan Univ Chinese Med, Coll Pharm, Changsha 410208, Peoples R China.;[Hu, Guangwan; Fan, Minxia; Guo, Mingquan] Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China.;[Hu, Guangwan; Fan, Minxia; Guo, Mingquan] Chinese Acad Sci, Innovat Acad Drug Discovery & Dev, Shanghai 201203, Peoples R China.
通讯机构：
[Mingquan Guo; Guangwan Hu] K;Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China<&wdkj&>College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China<&wdkj&>Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China<&wdkj&>Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences, Shanghai 201203, China
摘要：
Abstract: Polygonatum sibiricum Red. (P. sibiricum) has been used as a traditional Chinese medicine with a wide range of pharmacology effects. However, the responsible bioactive compounds and their mechanisms of action concerning its antioxidative and anti-hyperuricemic activities remain unexplored. In this work, the antioxidant capacity of P. sibiricum was firstly evaluated with the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azinobis-(3ethylbenzthiazoline)-6-sulfonic acid (ABTS) and ferric-reducing antioxidant power (FRAP) assays, from which the ethyl acetate (EA) fraction exhibited the highest DPPH, ABTS radical scavenging, and ferric-reducing capacities. Meanwhile, the EA fraction displayed the highest total phenolic and flavonoid contents among the four fractions. Next, the potential ligands from the EA fraction were screened out by bio-affinity ultrafiltration liquid chromatography-mass spectrometry (UF-LC-MS) with superoxide dismutase (SOD) and xanthine oxidase (XOD). As a result, N-trans-p-coumaroyloctopamine, N-trans-feruloyloctopamine, N-trans-feruloyltyramine were identified as potential SOD ligands, while N-cis-p-coumaroyltyramine was determined as potential XOD ligand. Additionally, these four ligands effectively interact with SOD and XOD in the molecular docking analysis, with binding energies (BEs) ranging from –6.83 to –6.51 kcal/mol, and the inhibition constants (Ki) from 9.83 to 16.83 μM, which were better than the positive controls. In conclusion, our results indicated that P. sibiricum has good antioxidative and anti-hyperuricemic activities, and its corresponding active ligands targeting SOD and XOD could be explored by the UF-LC-MS method. Keywords: Polygonatum sibiricum; UF-LC-MS; antioxidative; anti-hyperuricemic; SOD; XOD
摘要：
Background and Purpose: Irritable bowel syndrome (IBS) is usually associated with chronic gastrointestinal disorders. Its most common subtype is accompanied with diarrhea (IBS-D). The enteric nervous system (ENS) modulates major gastrointestinal motility and functions whose aberration may induce IBS-D. The enteric neurons are susceptible to long-term neurotransmitter level alterations. The patchouli alcohol (PA), extracted from Pogostemonis Herba, has been reported to regulate neurotransmitter release in the ENS, while its effectiveness against IBS-D and the underlying mechanism remain unknown. Experimental Approach: In this study, we established an IBS-D model in rats through chronic restraint stress. We administered the rats with 5, 10, and 20 mg/kg of PA for intestinal and visceral examinations. The longitudinal muscle myenteric plexus (LMMP) neurons were further immunohistochemically stained for quantitative, morphological, and neurotransmitters analyses. Key Results: We found that PA decreased visceral sensitivity, diarrhea symptoms and intestinal transit in the IBS-D rats. Meanwhile, 10 and 20 mg/kg of PA significantly reduced the proportion of excitatory LMMP neurons in the distal colon, decreased the number of acetylcholine (Ach)- and substance P (SP)-positive neurons in the distal colon and restored the levels of Ach and SP in the IBS-D rats. Conclusion and Implications: These findings indicated that PA modulated LMMP excitatory neuron activities, improved intestinal motility and alleviated IBS-induced diarrheal symptoms, suggesting the potential therapeutic efficacy of PA against IBS-D.
摘要：
Photodynamic therapy (PDT) provides apparent survival benefits for unresectable cholangiocarcinoma patients. the insufficient sensitivity of cancer cell to PDT treatment limits the clinical application. In this study, according to the GEO datasets, WNT7B expression was decreased by PDT treatment in cholangiocarcinoma samples. In cholangiocarcinoma cells, PDT treatment inhibited Wnt signaling, suppressed cell viability, and enhanced cell apoptosis. Within cholangiocarcinoma cells, PDT treatment induced p53 and miR-34a-5p expression. Under PDT treatment, p53 knockdown downregulated miR-34a-5p expression, whereas the inhibition effect of p53 knockdown on miR-34a-5p could be partially attenuated by agomir-34a-5p. p53 knockdown enhanced cell viability and suppressed cell apoptosis, whereas miR-34a-5p overexpression exerted opposite effects; miR-34a-5p overexpression partially attenuated p53 knockdown effects on PDT-treated cholangiocarcinoma cells. miR-34a-5p directly targeted WNT7B and inhibited WNT7B expression. Under PDT treatment, WNT7B knockdown inhibited the Wnt signaling and cell viability, and promoted cell apoptosis, while miR-34a-5p suppression showed the opposite trends; WNT7B knockdown partially attenuated miR-34a-5p inhibition effects on PDT-treated cholangiocarcinoma cells. In conclusion, PDT treatment induces p53-induced miR-34a transactivation to inhibit cholangiocarcinoma cell proliferation; the miR-34a-5p/WNT7B axis and Wnt signaling are involved. (C) 2021 Elsevier Inc. All rights reserved.
摘要：
Journal of Empirical Research on Human Research Ethics, Volume 17, Issue 3, Page 362-372, July 2022. <br/>This study aims to investigate the knowledge and attitudes of participants and potential participants in clinical trials toward electronic informed consent. We conducted a survey-based cross-sectional study in Hunan Province, China in March 2021. A total of 547 respondents were included in this study. All questions in an 8-item survey section assessing participants’ knowledge of electronic informed consent received correct answers from at least 70% of participants. In terms of attitude scores, most participants (86.3%) believed that electronic informed consent is more convenient than the paper-based version, and more than half (51.2%) believed that electronic informed consent could completely replace the paper-based version. Responses indicated that common concerns about electronic informed consent were its security and confidentiality, legal benefits, and implications for rights protection.
摘要：
Six flavonoids, namely, three undescribed biflavonoids, one undescribed 8-aryl flavonoid, and two known compounds, were isolated from Selaginella tamariscina (P.Beauv.) Spring. The structures and absolute configurations of those undescribed compounds were established by NMR spectroscopy data, HRESIMS analyses and electronic circular dichroism (ECD) analyses. In addition, all the isolates were evaluated for their hypoglycemic activity in HepG2 cells. Involvenflavone H, I, and J significantly increased glucose consumption in both normal and insulin-resistant HepG2 cells. Interestingly, these three compounds can effectively upregulate the protein expression of glucokinase (GCK) and adenylate cyclases (ADCYs). These results suggested that involvenflavone H, I, and J (especially involvenflavone J) may have potent hypoglycemic activity, which also provided promising molecular targets for the treatment of diabetes.
摘要：
Diabetes mellitus increases the risk of dementia, and evidence suggests hyperglycemia is a key contributor to neurodegeneration. However, our understanding of diabetes-associated cognitive decline, an important complication of diabetes mellitus, is lacking and the underlying mechanism is unclear. Blood brain barrier (BBB) breakdown is a possible cause of dementia in diabetes mellitus and Alzheimer's disease. Accumulating evidence shows BBB dysfunction caused by hyperglycemia contributes to cognitive decline. A specific type of inflammatory programmed cell death, called pyroptosis, has potential as a therapeutic target for BBB-associated diseases. Potential inducers of pyroptosis include inflammasomes such as NLRP3, whose activation relies on damage-associated molecular patterns. High mobility group box 1 (HMGB1) is a highly conserved, ubiquitous protein found in most cell types, and acts as a damage-associated molecular pattern when released from the nucleus. We propose that HMGB1 influences vascular inflammation by activating the NLRP3 inflammasome and thereby initiating pyroptosis in vascular cells. Moreover, HMGB1 plays a pivotal role in the pathogenesis of diabetes mellitus and diabetic complications. Here, we review the role of HMGB1 in BBB dysfunction induced by hyperglycemia and propose that HMGB1 is a promising therapeutic target for countering diabetes-associated cognitive decline.
作者机构：
[Jiao, Liqun; Xu, Ran; Yang, Bin; Wang, Tao; Luo, Jichang; Ma, Yan] China Int Neurosci Inst China INI, Beijing, Peoples R China.;[Jiao, Liqun; Xu, Ran; Yang, Bin; Wang, Tao; Luo, Jichang; Ma, Yan] Capital Med Univ, Dept Neurosurg, Xuanwu Hosp, Beijing, Peoples R China.;[Liao, Wanying] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Beijing, Peoples R China.;[Wang, Xue] Capital Med Univ, Xuanwu Hosp, Med Lib, Beijing, Peoples R China.;[Li, Wei] Liaocheng Peoples Hosp, Dept Neurosurg, Liaocheng, Shandong, Peoples R China.
通讯机构：
[Yang, B.; Jiao, L.] C;China International Neuroscience Institute (China-INI)China
关键词：
CLINICAL PHARMACOLOGY;Coronary heart disease;Lipid disorders;Protocols & guidelines;VASCULAR MEDICINE
期刊：
Frontiers in Cellular and Infection Microbiology,2022年12:1004845 ISSN：2235-2988
作者机构：
[Xiao, Nenqun; Zhou, Kang; Peng, Maijiao; Yi, Xin] Hunan Univ Chinese Med, Coll Pharm, Changsha, Peoples R China.;[Cai, Ying; Deng, Na] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Peoples R China.
关键词：
Baohe pill decoction;lactase-producing bacteria;high-fat and high-protein diet;Diarrhea;intestinal content
摘要：
Background: This study investigated the effects of Baohe pill decoction on the diversity and community composition of lactase-producing bacteria in the intestinal contents of mice with diarrhea induced by high-fat and high-protein diet, which provided an experimental basis for the study on the therapeutic mechanism of Baohe pill decoction. Materials and methods: The Traditional Chinese Medicine Systems Pharmacology (TCMSP), DisGeNET, UniProt, National Center for Biotechnology Information (NCBI), and GeneCards databases were used to collect the potential targets with active ingredients of Baohe pill decoction, diarrhea, and lactase, and then construct correlation networks. Fifteen Kunming mice were randomly divided into the control group (CN), natural recovery group (NR), and Baohe pill decoction treatment group (BHP), with five mice in each group. After constructing a mouse diarrhea model by HFHPD induction, BHP was gavaged with Baohe pill decoction, and the other groups were gavaged with distilled water of equal. The intestinal contents were collected from ileal to jejunal and analyzed using metagenomic sequencing to characterize the intestinal content of lactase-producing bacteria in mice. Results: The core active ingredients related to diarrhea in Baohe pill decoction were quercetin, luteolin, kaempferol, forsythin, and wogonin. And there was no intersection between the potential targets with the active ingredient of Baohe pill, lactase, and diarrhea. After the intervention of Baohe pill decoction, the Observed species, Chao1 index, and Operational Taxonomic Units (OTU) number increased in BHP (P > 0.05), while the Pielous evenness and Shannon index decreased (P > 0.05). In Beta diversity, the community structure of the NR was significantly different from CN and BHP (P < 0.05), and the community structure of the CN was not significant difference from BHP (P > 0.05). Compared to NR, the relative abundance of Bifidobacterium and Amycolatopsis increased, while the relative abundance of Lachnoclostridium, Sinorhizobium, Cedecea, and Escherichia decreased in BHP, but none of the significant differences (P > 0.05). Conclusion: The therapeutic effect of Baohe pill decoction on diarrhea induced by HFHPD does not appear to involve the body's lactase gene targets directly, but is associated with the change of the construction of lactase-producing bacterial communities.
