作者机构：
[Yuan, Xin; Wang, Tuanmei; Wang, Guohong; Yan, Shuyuan; Wang, Yanling; He, Jun; Peng, Xiangwen; Wang, Dongbo] Hunan Normal Univ, Changsha Hosp Maternal & Child Hlth Care, Changsha 410000, Peoples R China.;[Yuan, Xin] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Peng, Xiangwen] H;Hunan Normal Univ, Changsha Hosp Maternal & Child Hlth Care, Changsha 410000, Peoples R China.
关键词：
ASXL1;IVF;whole-exome sequencing
摘要：
RATIONALE: Bohring-Opitz syndrome is a severe congenital disorder associated with a de novo mutation in the additional sex combs-like 1 (ASXL1) gene, and it is characterized by symptoms that include developmental delay and musculoskeletal and neurological features. PATIENT CONCERNS: The patient was a girl, an in vitro fertilization (IVF) baby, with delayed motor development, drooling, short stature, slow growth, low muscle tone, image diagnosis of hypoplasia of the corpus callosum, delayed tooth eruption, high palatal arch, adduction of the thumb, drooling, not chewing, excessive joint activity, and ligament relaxation. DIAGNOSIS: Whole-exome sequencing analysis detected 1 novel disruptive frameshift mutation in ASXL1 in the proband but wild-type ASXL1 in both parents. INTERVENTIONS: Approximately 1 year of rehabilitation training, which included exercise therapy, toy imitation operation, cognition of daily objects, daily living skills training, gesture language training, oral muscle training, and hand movement training. OUTCOMES: After approximately 1 year of training, the patient was 3 years old and able to eat normally without drooling. She was able to grasp objects and pick them up after they fell. She was able to grasp small objects and actively played with toys. In addition, she was able to crawl on the floor (at slow speed, with poor initiative), stand with assistance, and walk with assistance; she was unstable when standing unassisted (standing unassisted for 8 seconds at most during training). LESSON: ASXL1 c.3762delT is a novel mutation that may be caused by IVF. This finding suggests that appropriate gene mutation detection approaches may be necessary for IVF technology.
作者：
Su, Yuting;Peng, Yao;Ren, Jie;Wu, Shangjie;Lei, Si;...
期刊：
Journal of Analytical Methods in Chemistry,2022年2022 ISSN：2090-8865
作者机构：
[Peng, Yao; Ren, Jie; Su, Yuting; Li, Juan; Li, Shunxiang] Hunan Univ Chinese Med, Hunan Engn Technol Res Ctr Bioact Subst Discovery, Sch Pharm, Changsha 410208, Peoples R China.;[Peng, Yao; Ren, Jie; Su, Yuting; Li, Juan; Li, Shunxiang] Hunan Prov Sino US Int Joint Res Ctr Therapeut Dru, Changsha 410208, Peoples R China.;[Wu, Shangjie; Lei, Si; Peng, Fei] Cent South Univ, XiangYa Hosp 2, Changsha 410008, Peoples R China.;[Sun, Zhina; He, Xiuqing] Qinghai Red Cross Hosp, Xining 810099, Peoples R China.
摘要：
Berberidis Cortex is rich in alkaloids, and many of them have antibacterial, anti-inflammatory, and hypoglycemic activities. However, few research studies have focused on the quantitative analysis of multiple components from Berberidis Cortex. In this study, a new quality evaluation strategy for Berberidis Cortex was developed and validated by high-performance liquid chromatography (HPLC), which involved single marker, fingerprint, and stoichiometric methods. Using berberine hydrochloride as an internal reference, the relative correction factors of palmatine hydrochloride, magnoline, and jatrorrhizine hydrochloride were 2.4537, 0.9783, and 1.0035, respectively, and their durabilities were also well performed. In addition, both methods mentioned above were used to compare the mass fractions of four isoquinoline alkaloids in ten batches of Berberidis Cortex from different origins. These results indicated that the approach applied in this study was accurate and feasible. The fingerprints of these ten batches of Berberidis Cortex were established, and eleven components were identified with the similarity greater than 0.993. Both cluster and principal component analysis were carried out based on the peak area of these components, the results demonstrated that these ten batches of Berberidis Cortex were divided into two groups and the distribution of the medicinal material was basically consistent. Therefore, quantitative analysis of multicomponents by single marker (QAMS) can be widely used in the quality control of Berberidis Cortex as theoretical basis.
期刊：
Journal of Ethnopharmacology,2022年295:115357 ISSN：0378-8741
通讯作者：
Ge, J.
作者机构：
[Deng, Xianguang; Liu, Lifang; Zhao, Da; Ge, Anqi; Ge, Jinwen] First Hosp Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Yang, Kailin; Ge, Jinwen] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Hunan, Peoples R China.;[Ge, Jinwen] Hunan Acad Chinese Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Anqi Ge; Lifang Liu] T;[Kailin Yang; Jinwen Ge] K;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>Hunan Academy of Chinese Medicine, Changsha, Hunan Province, China<&wdkj&>The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, China
关键词：
Breast cancer;Meta-analysis;Randomized controlled trial;Systematic review;Xihuang capsule;Xihuang pill
期刊：
Frontiers in Pharmacology,2022年13:2067 ISSN：1663-9812
通讯作者：
Fu, W.
作者机构：
[You, Xujun] Hunan Univ Chinese Med, Grad Sch, Changsha, Peoples R China.;[Li, Qixin; You, Xujun; Fu, Wei] Guangzhou Univ Chinese Med, Shenzhen Baoan Tradit Chinese Med Hosp, Dept Androl, Shenzhen, Peoples R China.;[Wu, Yongrong] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Peoples R China.;[Sheng, Wen] Hunan Univ Chinese Med, Androl Lab, Changsha, Peoples R China.;[Zhou, Qing] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Androl, Changsha, Peoples R China.
通讯机构：
Department of Andrology, Shenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, China
关键词：
Astragalus-Scorpio;prostate cancer;PI3K;AKT;Astragaloside IV;polypeptide extract from scorpion venom;autophagy
摘要：
Superb microvascular imaging (SMI) is among the latest doppler ultrasound methods. It uses an advanced clutter filter to eliminate artifacts caused by breathing, movement and retains the low-speed blood signals in microvessels. The great advantage of SMI is that it can intuitively detect very slow blood signals in microvessels, providing clinicians with more significant information about flow distribution in the target area. Therefore, it is speculated that SMI has important application value. The purpose of this article is to outline the application of SMI in different parts of the body.
摘要：
In recent years, manganese dioxide (MnO2) nanoparticles with unique physicochemical properties have been widely used in many biomedical fields, such as biosensors, contrast agents, tumor therapy, and drug delivery. From these applications, MnO2 nanoparticles have great clinical translation potential. However, by contrast, the in vitro and in vivo biosafety of MnO2 nanoparticles have been deeply and thoroughly clarified for the clinical translation, which hinders their clinical applications. In this work, we deeply investigated the blood safety of MnO2 nanoparticles by conducting a series of in vitro and in vivo experiments. These included the effects of MnO2 nanoparticles on morphology of red blood cells, activation of platelets, coagulation functions, and toxicity of key organs. The obtained results show that these effects displayed a concentration-dependent manner of MnO2 nanoparticles. Different safe concentration ranges could be found in the different experimental index. This study provides important guidance for the specific biomedical applications of MnO2 nanoparticles, greatly accelerating their laboratory development and clinical translation.
摘要：
Lilium, a perennial crop with great ornamental, medicinal and edible value, has been frequently used as functional food and medicine. Lilium lancifolium Thunb. (L. lancifolium) and Lilium brownii F.E.Brown var.viridulum Baker (L. brownii) are the most used medicinal species in China. However, the flavor compounds of these two species have not yet been clear. Here, metabolomics and transcriptome analysis were used to reveal the difference of the bitter substances of L. lancifolium and L. brownii. Qualitative results indicated that nine compounds are commonly existed in L. lancifolium and L. brownii, while nine compounds are unique in L. lancifolium and eight compounds are unique in L. brownii. Furthermore, quantitative results revealed that the content of regaloside A in L. lancifolium was nearly 2-7 folds higher than that of L. brownii, and the content of regaloside B in L. lancifolium was about 4-16 folds higher than that of L. brownii. Regaloside C and E were not detected in L. brownii. Transcriptome analysis showed that there were 90 unique genes up-regulated in L. lancifolium samples in the pathway of phenylpropanoid biosynthesis and 75 unique genes up-regulated in L. brownii samples, which could be related to the different content and chemical structure specificity of phenylpropanoid glycerol glucosides in L. lancifolium and L. brownii. The results of our in-deep research provide new insights into the bitter substances of L. lancifolium and L. brownii, and a further consideration for the chemical consistency and quality evaluation for Lilii bulbus.
期刊：
Journal of Healthcare Engineering,2022年2022 ISSN：2040-2295
作者机构：
[Chen, Xi; Gong, Juan; Tang, Xueyong; Jiang, Yourang; Qi, Dongwei] Chongqing Hosp Tradit Chinese Med, Dept Dermatol, Chongqing 400021, Peoples R China.;[Li, Xin] Hunan Univ Chinese Med, Changsha 410208, Peoples R China.
摘要：
Traditional Chinese medicine classifies psoriasis (Ps) according to clinical manifestations, and its different clinical manifestations imply the pathogenesis and material evolution basis of Ps, especially biomarkers that are meaningful to identification of Ps, treatment response, and elucidation of the pathogenesis of the disease. This study aims to screen differential metabolites in plasma of psoriasis vulgaris (PV) of blood heat syndrome based on a widely targeted metabolomic technique and to analyze syndrome metabolic markers and metabolic pathways. Forty-five PV patients were recruited, including 21 cases of the blood heat syndrome group (BH-PPG), 24 cases of the non-blood-heat syndrome group (NBH-PPG), and 30 healthy cases of the normal control group (NPG). The UPLC-MS/MS detection platform, a self-developed database, and multivariate statistical analysis were applied to investigate the plasma metabolic differences. The biomarkers related to blood heat syndrome were screened using the principal component analysis method. A total of 479 metabolites were detected in the three groups of plasma samples; 72 different metabolites were sorted out in the BH-PPG/NPG group, 82 in the NBH-PPG/NPG group, and 8 in the BH-PPG/NBH-PPG group. Differential metabolites mainly consist of metabolites of organic acids, amino acids, carbohydrates, and nucleotides. Multiple metabolites ginkgolic acid, pyrroloquinoline quinone, L-aspartic acid, and citramalic acid were expected to be the potential biomarkers of blood heat syndrome PV. The formation and evolution processes may be associated with disorders and regulation of metabolic pathways, ferroptosis, carbon metabolism, and purine metabolism.
期刊：
Evidence-Based Complementary and Alternative Medicine,2022年2022 ISSN：1741-427X
通讯作者：
Li, J.
作者机构：
[Zhang, Zexin] Guangzhou Univ Chinese Med, Clin Med Coll 1, Guangzhou 510405, Peoples R China.;[Liu, Zifeng; Lin, Siqi] Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou 510405, Peoples R China.;[Han, Jun] Beijing Tcmages Pharmaceut Co Ltd, Natl & Local Joint Engn Res Ctr Key Technol Chine, Beijing 101301, Peoples R China.;[Yu, Yi; Li, Jing] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410000, Peoples R China.
通讯机构：
The First Affiliated Hospital Of Hunan University Of Chinese Medicine, Changsha, China
摘要：
<i>Background</i>. Tao-He-Cheng-Qi Formula (THCQF) is a traditional Chinese medicine that has been proven to have antitumor effects. The aim of this study was to elucidate the molecular targets and mechanisms of THCQF against colon cancer and construct a prognostic model based on network pharmacology, bioinformatics analysis, and in vitro experiments. <i>Methods</i>. Potential THCQF compounds and targets were retrieved from the Traditional Chinese Medicine Systems Pharmacology and Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine databases. Differentially expressed genes for colon cancer were screened in The Cancer Genome Atlas and Gene Expression Omnibus databases. The anticolon cancer mechanisms of THCQF were explored using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Molecular docking simulations and molecular dynamics analysis were used to evaluate the binding between target proteins and active compounds. Finally, the identified compounds were used to treat colon cancer cells from the HCT116 cell line, and expression of mRNA and protein after relevant posttreatment were tested using real-time polymerase chain reaction and western blotting. <i>Results</i>. A total of 27 anticolon cancer targets of THCQF were selected, among which four genes (<i>CCNB1</i>, <i>CCNA2</i>, <i>IL1A</i>, and <i>MMP3</i>) were shown to effectively predict patient outcomes in a prognostic colon cancer model. GO and KEGG enrichment analyses indicated that the activity against colon cancer of THCQF was associated with the interleukin (IL)-4 and IL-3 signaling pathways. Two compounds in THCQF, aloe emodin (AE) and quercetin (QR), were shown to efficiently bind to cyclin B1, the protein encoded by <i>CCNB1</i>. Finally, incubation of HCT116 cells with AE and QR significantly decreased <i>CCNB1</i> mRNA expression and cyclin B1 levels. <i>Conclusions</i>. Taken together, the results indicate that AE and QR are the pivotal active compounds of THCQF, and <i>CCNB1</i> is the main molecular target through which THCQF exerts its anticolon cancer effects. The study findings provide insight for studies investigating the anticancer effects of other traditional Chinese medicines.
作者机构：
[Tan, Yang; Li, Qing; Liu, Xiang-Dan; Zhu, Xiang-Mei; Shi, Yu-He; Zhu, Jue; Tong, Qiao-Zhen] Hunan Univ Chinese Med, Pharm Coll, Xueshi Rd,300, Changsha 410208, Hunan, Peoples R China.;[Zhu, Xiang-Mei] Second Hosp Hunan Univ Chinese Med, Caie North Rd,233, Changsha 410005, Hunan, Peoples R China.;[Liu, Xiang-Dan; Tong, Qiao-Zhen] Key Lab Germplasm Resources & Standardized Plantin, Xueshi Rd,300, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Qiao-Zhen Tong] P;Pharmacy of College, Hunan University of Chinese Medicine, Changsha, Hunan 410208, People’s Republic of China<&wdkj&>Key Laboratory of Germplasm Resources and Standardized Planting of Bulk Authentic Medicinal Materials from Hunan, Changsha, Hunan 410208, People’s Republic of China
关键词：
Myocardial ischemia-reperfusion injury (MIRI);Tandem mass tag (TMT);Jiawei Danshen decoction (JWDSD);Proteomic;Traditional Chinese medicine (TCM)
摘要：
Every year, approximately 17 million people worldwide die due to coronary heart disease, with China ranking second in terms of the death toll. Myocardial ischemia-reperfusion injury (MIRI) significantly influences cardiac function and prognosis in cardiac surgery patients. Jiawei Danshen Decoction (JWDSD) is a traditional Chinese herbal prescription that has been used clinically for many years in China to treat MIRI. The underlying molecular mechanisms, however, remain unknown. To investigate the proteomic changes in myocardial tissue of rats given JWDSD for MIRI therapy-based proteomics. MIRI rat model was created by ligating/releasing the left anterior descending coronary artery. For seven days, the drugs were administered twice daily. The model was created following the last drug administration. JWDSD's efficacy in improving MIRI was evaluated using biochemical markers and cardiac histology. Tandem mass tag-based quantitative proteomics (TMT) technology was also used to detect proteins in the extracted heart tissue. To analyze differentially expressed proteins (DEPs), bioinformatics analysis, including gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways, were employed. Furthermore, western blotting confirmed the potential targets regulated by JWDSD. The histopathologic characteristics and biochemical data showed JWDSD's protective effects on MIRI rats. A total of 4549 proteins were identified with FDR (false discovery rate) ≤1%. Twenty overlapping were identified (162 DEPs and 45 DEPs in Model/Control or JWDSD/Model group, respectively). Of these DEPs, 16 were regulated by JWDSD. GO analysis provided a summary of the deregulated protein expression in the categories of biological process (BP), cell component (CC), and molecular function (MF). KEGG enrichment analysis revealed that the signaling pathways of neutrophil extracellular trap formation, RNA polymerase, serotonergic synapse, and linoleic acid metabolism are all closely related to JWDSD effects in MIRI rats. Furthermore, T-cell lymphoma invasion and metastasis 1 (TIAM1) was validated using western blotting, and the results were consistent with proteomics data. Our study suggests that JWDSD may exert therapeutic effects through multi-pathways regulation in MIRI treatment. This work may provide proteomics clues for continuing research on JWDSD in treating MIRI.
期刊：
IEEE Transactions on Industrial Informatics,2022年18(4):2877-2889 ISSN：1551-3203
通讯作者：
Xiong, NN
作者机构：
[Liang, Jingpu; Liu, Anfeng] Cent South Univ, Sch Comp Sci & Engn, Changsha 410083, Peoples R China.;[Liu, Wei] Hunan Univ Chinese Med, Sch Informat, Changsha 410208, Peoples R China.;[Xiong, Neal N.; Xiong, NN] Sul Ross State Univ, Dept Comp Sci & Math, Alpine, TX 79830 USA.;[Zhang, Shaobo] Hunan Univ Sci & Technol, Sch Comp Sci & Engn, Xiangtan 411201, Peoples R China.
通讯机构：
[Xiong, NN ] S;Sul Ross State Univ, Dept Comp Sci & Math, Alpine, TX 79830 USA.
关键词：
Codes;Industrial Internet of Things;Costs;5G mobile communication;Security;Informatics;Trajectory optimization;5G-enabled intelligent applications;code dissemination;industrial Internet of things (IIoT);trajectory optimization;trust evaluation
摘要：
A large number of devices with communication and sensing capabilities are connected to the 5G network and facilitate distributed industrial Internet of Things (IIoT) applications. Those devices can update their program code by using software-defined technologies and upgrade functions without hardware replacement, thus, greatly facilitating the development of IIoT applications. Disseminating code safely to these devices is a pivotal issue. However, the existing methods rarely consider whether the code received by devices is integrated. Therefore, an unmanned aerial vehicle (UAV) assisted trustworthy code dissemination (UTCD) framework in 5G-enabled intelligent IIoT systems is proposed to select credible mobile vehicles (MVs) to disseminate code with opportunistic routing style. In the UTCD framework, a verifiable trust evaluation scheme is proposed to identify the trust of the MVs by sending the UAV to collect the code wait to be verified (CWV) directly from the selected devices (bedrock devices) as evidence. This scheme is a fundamental change from the previous passive, indirect and unverifiable trust evaluation schemes. In UTCD, a bedrock devices selection scheme is presented by selecting as few bedrock devices as possible to collect as much the CWV as possible with the minimum cost. After that, based on the CWV, which is collected from bedrock devices, a complete trust evaluation scheme is proposed to obtain the trust of MVs. Furthermore, a UAV trajectory optimization algorithm is proposed to obtain as much CWV as possible within the limited conditions of UAV. Finally, comprehensive experiments conduct on a real-life dataset demonstrate that the proposed scheme outperforms the existing schemes in terms of the efficiency and security of code dissemination.
摘要：
Abstract Turpiniae Folium, the dried leaves of Turpinia arguta Seem., is a kind of historic traditional Chinese medicine. Here, based on our previous study, we extracted the Turpiniae Folium polysaccharides (TFP) and isolated three polysaccharide fractions from TFP. Then, TFP and one of the major polysaccharide fractions (TFP‐1a) were identified through HPLC, HPGPC, and ATR‐FTIR. Furthermore, the evaluations of their antioxidative, anti‐inflammatory activities and inhibitory effect on angiotensin II‐induced vascular smooth muscle cells (VSCMs) proliferation in vitro were conducted. Both TFP and TFP‐1a showed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities, and exerted strong anti‐inflammatory activity. Moreover, TFP and TFP‐1a also possessed a strong inhibitory effect on Ang II‐induced VSCMs proliferation. On these premises, we inferred that TFP and TFP‐1a could be potential and promising natural antioxidants, anti‐inflammatory agents, and implicated to treat cardiovascular disease.
摘要：
The high level of serum cholesterol caused by the excessive absorption of cholesterol can lead to hypercholesteremia, thus promoting the occurrence and development of cancer. Ezetimibe is a drug that reduces cholesterol absorption and has been widely used for the treatment of patients with high circulating cholesterol levels for many years. Mechanistically, ezetimibe works by binding to NPC1L1, which is a key mediator of cholesterol absorption. Accumulating data from preclinical models have shown that ezetimibe alone could inhibit the development and progression of cancer through a variety of mechanisms, including anti-angiogenesis, stem cell suppression, anti-inflammation, immune enhancement and anti-proliferation. In the past decade, there has been heated discussion on whether ezetimibe combined with statins will increase the risk of cancer. At present, more and more evidence shows that ezetimibe does not increase the risk of cancers, which supports the role of ezetimibe in anti-cancer. In this review, we discussed the latest progress in the anti-cancer properties of ezetimibe and elucidated its underlying molecular mechanisms. Finally, we highlighted the potential of ezetimibe as a therapeutic agent in future cancer treatment and prevention.
摘要：
Sex hormone secretion difference is one of the main reasons for sexually dimorphic traits in animals, which affects the dimorphism of the intestinal microbiota; however, their interaction is still unknown. Intestinal mucosa-associated microbiota (MAM) and intestinal luminal content microbiota (LM) belong to two different habitats according to the difference in interactions between bacteria and host intestinal epithelium/nutrients. To clarify the sexually dimorphic characteristics of MAM and LM and their correlation with sex hormones, 12 specific pathogen-free (SPF) Kunming mice from the same nest were fed separately according to sex. After 8 weeks, samples from the male intestinal mucosa group (MM group), the female intestinal mucosa group (FM group), the male intestinal content group (MC group), and the female intestinal content group (FC group) were collected and then, the next-generation sequencing of 16S ribosomal ribonucleic acid (rRNA) gene was performed. Our results showed that the sexual dimorphism of MAM was more obvious than that of LM and the relative abundance of Muribaculaceae, Turicibacter, and Parasutterella was significantly higher in the FM group than in the MM group (p < 0.001, p < 0.05, p < 0.05). Next, we measured the level of serum sex hormones in mice and calculated the correlation coefficient between major bacteria and sex hormones. The results showed that the correlation between MAM and sex hormones was more prominent, and finally, three bacterial genera (Muribaculaceae, Turicibacter, and Parasutterella) were obtained, which could better represent the relationship between sexual dimorphism and sex hormones. The abundance of Parasutterella is positively and negatively correlated with estradiol and testosterone (T), respectively, which may be related to the differences in the metabolism of bile acid and glucose. A decrease in the abundance of Turicibacter is closely related to autism. Our results show that the abundance of Turicibacter is negatively and positively correlated with T and estradiol, respectively, which can provide a hint for the prevalence of male autism. In conclusion, it is proposed in our study that intestinal microbiota is probably the biological basis of physiological and pathological differences due to sex, and intestinal MAM can better represent the sexual dimorphism of mice.
摘要：
Interleukin 10 (IL-10)-producing B cells (B10 cells) are a canonical cell fraction for regulating other activities of immune cells. Posttranscriptional modification of IL-10 in B10 cells is not yet fully understood. Short-chain fatty acids play an important role to regulate the functions of immune cells. This study aims to clarify the role of propionic acid (PA), a short-chain fatty acid, in regulating the expression of IL-10 in B10 cells. Blood samples were collected from patients with food allergy (FA) and healthy subjects. Serum and cellular components were prepared with the samples, and analysed by enzyme-linked immunosorbent assay and flow cytometry, respectively. The results showed that serum PA levels were lower in FA patients. PA concentrations were negatively correlated with serum cytokine Th2 concentrations, specific IgE concentrations in serum and skin prick test results. The peripheral frequency of B10 cells and the production of IL-10 in B cells were also associated with serum PA concentrations. Activation of B cells by CpG induced the production of IL-10 and tristetretrprolin (TTP), in which TTP caused the spontaneous decay of IL-10 mRNA. PA was necessary to stabilize the IL-10 mRNA in B cells by inducing the production of granzyme B, which resulted in the degradation of the IL-10 mRNA. Administration of PA attenuated FA response in mice by maintaining homeostasis of B10 cells. In conclusion, PA is needed to stabilize the expression of IL-10 in B10 cells. PA administration can mitigate experimental FA by maintaining B10 cell functions.
作者机构：
[Wang, Yu-Yan; Cheng, Fei; Wu, Jian-Ping; Xu, Kang-Ping; Li, Gui-Hua; Zhang, Yan] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410013, Peoples R China.;[Chen, Zu-Hui; Wang, Yu-Yan; Wu, Jian-Ping; Xu, Kang-Ping; Li, Gui-Hua] Hunan Acad Chinese Med, Inst Chinese Mat Med, Changsha 410013, Peoples R China.;[Deng, Gui-Ming; Long, Hong-Ping] Hunan Univ Chinese Med, Hosp 1, Changsha 410007, Peoples R China.;[Cheng, Fei; Zeng, Hong-Liang] Haikou Peoples Hosp, Haikou 570208, Hainan, Peoples R China.;[Cheng, Fei; Zeng, Hong-Liang] Cent South Univ, Xiangya Sch Med, Affiliated Haikou Hosp, Haikou 570208, Hainan, Peoples R China.
通讯机构：
[Fei Cheng; Kang-Ping Xu] X;Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013 P. R. China<&wdkj&>Haikou People's Hospital and Central South University Xiangya School of Medicine Affiliated Haikou Hospital, Haikou, 570208 P. R. China<&wdkj&>Hunan QingYa Health Service Limited Company, Changsha, 410000 P. R. China<&wdkj&>Hunan Key Laboratory of Diagnostic and Therapeutic Drug Research for Chronic Diseases, Central South University, Changsha, 410013 P. R. China<&wdkj&>Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013 P. R. China<&wdkj&>Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Changsha, 410013 P. R. China
摘要：
Abstract Two new C21 steroidal glycosides, brapreguanes A and B (1–2) were isolated from 75 % aqueous ethanol extract of Selaginella braunii Baker. Their structures were established by spectroscopic analyses (1D/2D NMR spectra and HR‐ESI‐MS). The absolute configurations of sugar were elucidated by enzymatic hydrolysis and GCMS analysis. In addition, all compounds were evaluated for the anti‐proliferative activities against various human cancer cells in vitro. Compounds exhibited no inhibition to various human cancer cells.
