摘要：
Objective To assess the effect and safety of Hydroxysafflor Yellow A for Injection (HSYAI) in treating patients with acute ischemic stroke (AIS) and blood stasis syndrome (BSS). Methods A multicenter, randomized, double-blind, multiple-dose, active-controlled phase II trial was conducted at 9 centers in China from July 2013 to September 2015. Patients with moderate or severe AIS and BSS were randomly assigned to low-, medium-, high-dose HSYAI groups (25, 50 and 70 mg/d HSYAI by intravenous infusion, respectively), and a control group (Dengzhan Xixin Injection (& x706f;& x76cf;(sic)& x8f9b;(sic)& x5c04;& x6db2;, DZXXI) 30 mL/d by intravenous infusion), for 14 consecutive days. The primary outcome was the Modified Rankin Scale (mRS) score <= 1 at days 90 after treatment. The secondary outcomes included the National Institute of Health Stroke Scale (NIHSS) score <= 1, Barthel Index (BI) score > 95, and BSS score reduced > 30% from baseline at days 14, 30, 60, and 90 after treatment. The safety outcomes included any adverse events during 90 days after treatment. Results Of the 266 patients included in the effectiveness analysis, 66, 67, 65 and 68 cases were in the low-, medium-, and high-dose HSYAI and control groups, respectively. The proportions of patients in the medium- and high-dose HSYAI groups with mRS score <= 1 at days 90 after treatment were significantly larger than the control group (P<0.05). The incidences of favorable outcomes of NIHSS and BI at days 90 after treatment as well as satisfactory improvement of BSS at days 30 and 60 after treatment in the medium- and high-dose HSYAI groups were all significantly higher than the control group (P<0.05). No significant difference was reported among the 4 groups in any specific adverse events (P>0.05). Conclusions HSYAI was safe and well-tolerated at all doses for treating AIS patients with BSS. The medium (50 mg/d) or high dose (75 mg/d) might be the optimal dose for a phase III trial. (Registration No. ChiCTR-2000029608)
作者机构：
[Zong-li Liao; Jing Tan; Lu Sun; Ren-da Yang; Xiao-rong Chang] Hunan University of Chinese Medicine, Changsha, China;[Chong-zheng Zhu] Huizhou Hospital of Traditional Chinese Medicine, Guangdong, China;[Wen-tao Huang] The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, China
通讯机构：
[Xiao-rong Chang] H;Hunan University of Chinese Medicine, Changsha, China
关键词：
Moxibustion Therapy;Indirect Moxibustion;Herbal Cake-partitioned Moxibustion;Hyperlipidemias;Laurocapram;Janus Kinase 2/Signal Transducer and Activator of Transcription 3 (JAK2/STAT 3) Pathway;Leptin;Rabbits
摘要：
MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil (CTVO) was found to facilitate MSC migration. Further exploration of the underlying molecular mechanism participating in the pro-migratory ability may provide a novel strategy to improve MSC transplantation efficacy. This study indicated that CTVO promotes MSC migration through enhancing ROCK2 mRNA and protein expressions. MSC migration induced by CTVO was blunted by ROCK2 inhibitor, which also decreased myosin light chain (MLC) phosphorylation. Meanwhile, the siRNA for ROCK2 inhibited the effect of CTVO on MSC migration ability and attenuated MLC phosphorylation, suggesting that CTVO may promote BMSC migration via the ROCK2/MLC signaling. Taken together, this study indicates that C. tinctorius volatile oil could enhance MSC migration via ROCK2/MLC signaling in vitro. C. tinctorius volatile oil-targeted therapy could be a beneficial strategy to improve the therapeutic potential of cell transplantation for bone diseases in regenerative medicine.
作者机构：
[De-sheng Zhang; Shui-chun Liu; Shao-qiu Sun] The Second Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410005, China (湖南中医药大学第二附属医院, 长沙410005, 中国);[Pei-rong Zhou] Hunan University of Traditional Chinese Medicine, Changsha 410208, China (湖南中医药大学, 长沙410208, 中国)
通讯机构：
[Shao-qiu Sun] T;The Second Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410005, China (湖南中医药大学第二附属医院, 长沙410005, 中国)
摘要：
Objective: To compare the clinical effect differences of bian (砭) stone hot compress combined with acupuncture at cervical Jiaji (夹脊EX-B2) acupoint and simple acupuncture at cervical EX-B2 for axial pain after cervical laminectomy with implant fixation (LIF) operation. Methods: A total of 80 patients with axial pain after cervical LIF operation were randomly assigned into the bian (砭) stone hot compress combined with acupuncture at cervical EX-B2 group (Group A) and the acupuncture at cervical EX-B2 group (Group B), and 40 patients in each group. The group A was treated by bian (砭) stone hot compress and acupuncture at cervical EX-B2, while the group B was treated by acupuncture at cervical EX-B2. Both of groups were treated once a day, one course of treatment consisted of 7 days, 6-day treatment plus 1-day rest, and there were 3 courses of treatment in total. The pain score (Visual analogue scale,VAS), neck dysfunction indices (NDI), and serum creatine phosphokinase (CPK) levels of patients were compared before and after the treatment, and their clinical effects were evaluated. Results: The VAS score, NDI, and CPK in patients of the two groups after treatment were all lower than those before treatment (all P<0.05), and the indicators in the group A after treatment were all lower than those in the group B after treatment (all P<0.05). The effective rate in the group A was 92.5%, higher than that (77.5%) in the group B, and there was statistically significant difference (P<0.05). Conclusions: The bian (砭) stone hot compress combined with acupuncture at cervical EX-B2 acupoint was better than acupuncture at cervical EX-B2 alone in treatment of axial pain after cervical LIF operation. In the group A, therapeutic effects were improved by relieving pain, improving neck function, and repairing muscle injury.
作者机构：
Hunan University of Chinese Medicine, Changsha, 410007;School of Medicine, Southeast University, Nanjing, 210009;Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410005;Brain Hospital of Hunan Province, Changsha, 410007;[Zhang Lin; Quan Shu-lin; Wang Jing-jing; Zhong Yan] Hunan University of Chinese Medicine, Changsha, 410007
通讯机构：
[Jing-jing Wang] H;Hunan University of Chinese Medicine, Changsha, China
摘要：
Objective: To observe the therapeutic efficacy of acupuncture plus Tai Ji Quan (Tai Chi) in recovering the neurological function and treating depression state in post-stroke depression patients, together with a 12-month follow-up. Methods: A total of 105 eligible post-stroke depression patients were randomized into an acupuncture plus Tai Ji group (53 cases) and a control group (52 cases) based on their visiting sequence. The patients all received routine treatment and rehabilitation training for stroke. In addition, the control group was given oral administration of citalopram hydrobromide tablets, 1 month as a course of treatment, for 3 courses in total. Meanwhile, the acupuncture plus Tai Ji group received acupuncture and practiced Tai Ji Quan, for 1 month and 12 months respectively. Before the intervention, after 1-month intervention and 12 months later, the National Institute of Health stroke scale (NIHSS), Barthel index (BI) and Hamilton depression rating scale (HAMD) were adopted for efficacy evaluation. Results: Prior to the intervention, there were no significant differences in HAMD, NIHSS and BI scores between the two groups (all P>0.05); after 1-month intervention, there were significant between-group differences in NIHSS, BI and HAMD scores (P<0.05 or P<0.01); the 12-month follow-up revealed significant between-group differences in NIHSS, BI and HAMD scores (all P<0.01). In the treatment of stroke, the total effective rate was 84.4 %in the acupuncture plus Tai Ji group, significantly higher than 68.9 %in the control group (P<0.05); in the treatment of depression, the total effective rate was 86.7 %in the acupuncture plus Tai Ji group, significantly higher than 77.8 %in the control group (P<0.05). Conclusion: Acupuncture plus Tai Ji Quan can produce a significant efficacy in improving the limb motor function and depression in post-stroke depression patients.
作者机构：
Hunan University of Chinese Medicine, Changsha, 410007;The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410005;Brain Hospital of Hunan Province, Changsha, 410007;[张林; 钟艳; 全淑林; 王净净] Hunan University of Chinese Medicine, Changsha, 410007;[石学慧] The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410005
通讯机构：
[Lin Zhang] H;Hunan University of Chinese Medicine, Changsha, China
关键词：
Acupuncture Therapy;Auricuar Point Sticking;Stroke;Complications;Heart Rate;Depression
摘要：
Objective: To investigate the effect of combining acupuncture and auricular point sticking on heart rate variability (HRV)in patients with post-stroke depression (PSD). Methods: A total of 80 cases with PSD were randomized into a treatment group and a control group. The control group was intervened by oral administration of paroxetine hydrochloride, whereas the treatment group received acupuncture plus auricular point sticking base on the same oral administration. The Hamilton depression rating scale (HAMD)and HRV were measured before and after treatment in both groups. Results: The individual and global scores of HAMD significantly dropped after 8 weeks of treatment in both groups (all P<0.05). In the treatment group, anxiety/somatization factor, sleep disturbance, hopelessness factor, cognition factor and global score were significantly different from those in the control group (all P<0.05). The 24 h standard deviation of all normal-to-normal R-R interval (SDNN), standard deviation of 5-minute average of normal R-R intervals (SDANN), root mean square of successive differences (RMSSD), percent of differences between adjacent normal R-R intervals >50 ms (PNN50)and high frequency (HF)were increased while low frequency (LF)and LF/HF decreased significantly after 8 weeks of treatment in both groups (P<0.05). All items in the treatment group were significantly different from those in the control group (all P<0.05). Conclusion: Combining acupuncture and auricular point sticking can enhance the conventional medical treatment for HRV in patients with PSD.
作者机构：
[钟鸣; 李湘璐; 杨崧] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;Key Disciplinary Areas of "The Twelfth Five-year Plan" in Hunan Province, Changsha, 410208, China;[王适] Second Clinical Medical College of Hunan University of Chinese Medicine, Changsha, 410005, China;[Lü Y.] Hunan Food and Drug Professional College, Changsha, 410208, China;[何群] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China, Key Disciplinary Areas of "The Twelfth Five-year Plan" in Hunan Province, Changsha, 410208, China
通讯机构：
[Yang, S.] C;College of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
