摘要：
BACKGROUND: Rosacea, a common chronic inflammatory skin disease worldwide, is currently incurable with complex pathogenesis. Dendrobium polysaccharide (DOP) may exert therapeutic effects on rosacea via acting on the NF-κB-related inflammatory and oxidative processes. MATERIALS AND METHODS: In this study, an LL-37-induced rosacea-like mouse model was established. HE staining was used to assess the skin lesions, erythema severity scores, pathological symptoms, and inflammatory cell numbers of mice in each group. The inflammation level was quantitatively analyzed using enzyme-linked immunosorbent assay (ELISA) and reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The expression levels of TLR4 and p-NF-κB were finally detected. RESULTS: DOP improved skin pathological symptoms of rosacea mice. DOP also alleviated the inflammation of rosacea mice. Moreover, the TLR4/NF-κB pathway was observed to be inhibited in the skin of mice after DOP application. These findings evidenced the anti-inflammatory effects of DOP on the LL-37-induced rosacea mouse model. DOP could inhibit NF-κB activation, suppress neutrophil infiltration, and reduce pro-inflammatory cytokines production, which may be the reason for DOP protecting against rosacea. CONCLUSION: This study may propose an active candidate with great potential for rosacea drug development and lay a solid experimental foundation for promoting DOP application in rosacea therapy.
期刊：
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY,2024年83(1):86-92 ISSN：0160-2446
通讯作者：
Liu, XL;Ye, HH
作者机构：
[Ma, Lili; Zhou, Hua; Zhang, Yuyu; Huang, Yun; Fang, Chongbo; Rong, Weibo] Ningbo Univ, Li Huili Hosp, Ningbo Med Ctr, Dept Pharm, Ningbo, Zhijiang, Peoples R China.;[Liu, Xiaoli] Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Caie North Rd 233, Changsha, Hunan, Peoples R China.;[Ye, Honghua] Ningbo Univ, Li Huili Hosp, Ningbo Med Ctr, Dept Cardiol, Xingning Rd 57, Ningbo 315040, Zhijiang, Peoples R China.;[Liu, Xiaoli] Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Caie North Rd 233, Changsha 410119, Hunan, Peoples R China.
通讯机构：
[Liu, XL ] H;[Ye, HH ] N;Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Caie North Rd 233, Changsha, Hunan, Peoples R China.;Ningbo Univ, Li Huili Hosp, Ningbo Med Ctr, Dept Cardiol, Xingning Rd 57, Ningbo 315040, Zhijiang, Peoples R China.;Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Caie North Rd 233, Changsha 410119, Hunan, Peoples R China.
关键词：
SGLT2 inhibitors, heart failure, cost-effectiveness, China
摘要：
Supplemental Digital Content is Available in the Text. This study aimed to compare the cost-effectiveness of the new quadruple therapy regimen of adding sodium-glucose-linked transporter 2 (SGLT2) inhibitors, with standard treatment for patients with heart failure (HF) in China. From the payer's perspective, the dates of cardiovascular event recurrences were extracted from a meta-analysis including 6 trials, combined with the treatment cost for patients with HF in China to construct a Markov model. The outcomes included per capita medical costs and incremental cost-effectiveness ratio, using quality-adjusted life years (QALYs) data. Single-factor, probability sensitivity analysis, and scenario analysis were used to explore the potential uncertainties of the model. The per capita costs of the new quadruple therapy regimen and standard treatment were $87441.26 and $87087.54, respectively. The new regimen was associated with a mean of 21.44 QALYs gained, compared with 18.60 QALYs gained with the standard treatment. The incremental cost-effectiveness ratio was $124.03 per QALY gained. The sensitivity analysis revealed that changes in the parameters within the set range did not affect the model results. In China, compared with standard treatment, the new quadruple therapy regimen with SGLT2 inhibitors reduce the frequency of cardiovascular events among patients with HF, and it has economic advantages.
作者机构：
[Zhou, Hao] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Urol, Changsha 410001, Hunan, Peoples R China.;[Wang, Fang] Changsha Social Work Coll, Med Coll, Changsha 410004, Hunan, Peoples R China.;[Wang, F; Wang, Fang] Changsha Social Work Coll, Med Coll, 22 XiangZhang Rd, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Wang, F ] C;Changsha Social Work Coll, Med Coll, 22 XiangZhang Rd, Changsha 410004, Hunan, Peoples R China.
摘要：
Hepatic leukemia factor (HLF), a transcription factor, is dysregulated in many cancers. This study investigates the function of HLF in prostate cancer (PCa) and its relation to tensin 1 (TNS1). Clinical tissues were collected from 24 PCa patients. Duke University 145 (DU145) and PC3 cells overexpressing HLF were established. HLF signaling was downregulated in PCa tissues compared to adjacent tissues and in DU145 and PC3 cells compared to prostate epithelial cells RWPE-1 or prostate stromal cells (WPMY-1). PCa cell lines with overexpression of HLF had reduced proliferative, migratory, and invasive activity, increased apoptosis, and cell mitosis mostly in the G0/G1 phase. HLF induced the TNS1 transcription to activate the p53 pathway. Depletion of TNS1 reversed the anti-tumor effects of HLF on PCa cells and tumor growth and metastasis in vivo. In summary, our findings suggest that HLF suppressed PCa progression by upregulating TNS1 expression and inducing the p53 pathway activation, which might provide insights into novel strategies for combating PCa.
期刊：
Frontiers in Psychology,2023年14:1150998 ISSN：1664-1078
通讯作者：
Zhu, SL
作者机构：
[Tang, Tao-Ran; Li, Hong] Hunan Univ Chinese Med, Sch Nursing, Changsha, Hunan, Peoples R China.;[Yang, Song-Chun; Wang, Mi; Ke, Xin-Chen; Zhang, Han-Yi; Su, Juan; Lin, Wen-Rui] Cent South Univ, Xiangya Hosp, Dept Dermatol, Changsha, Hunan, Peoples R China.;[Zhang, Cheng-Cheng] Cent South Univ, Sch Publ Hlth, Dept Social Med & Hlth Management, Changsha, Hunan, Peoples R China.;[Zhu, Shi-Lin] Hunan Univ Chinese Med, Affiliated Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhu, SL ] H;Hunan Univ Chinese Med, Affiliated Hosp 2, Changsha, Hunan, Peoples R China.
关键词：
anxiety;depression;mental health service;skin disease;survey;web-based
摘要：
OBJECTIVE: The study aimed to analyze the current status and reasons for the neglect of the psychological need of patients with common skin diseases. METHODS: This cross-sectional study was conducted in China using an online self-assessment questionnaire distributed via social media. Demographic, clinical and psychological data were collected, and the main outcomes, i.e., depression (evaluated using the 9-item Patient Health Questionnaire, PHQ-9) and anxiety (evaluated using Generalized Anxiety Disorder-7, GAD-7). Multivariate regression analysis was used for the prediction of variates of mental health service seeking behaviors. RESULTS: A total of 1,010 patients participated in the survey, and 273 (27.0%) patients met the "with need" criteria, i.e., having the need for mental health intervention but not being treated. In the multivariate regression model, income (OR = 0.80, 95%CI: 0.65-0.99), duration of disease (OR = 0.68, 95%CI: 0.49-0.95) and suicide ideation (OR = 2.10, 95%CI: 1.14-3.87) were significant factors. For patients who did not receive mental health care, the lack of knowledge about the availability of mental health services, lack of knowledge of where to seek help, concerns about the side effects of treatment, failure to seek treatment for severe skin diseases, and absence of current psychological distress were associated with their need for psychological intervention. CONCLUSION: This study examined the current status of the need for psychological intervention and the reasons why the need was unmet in patients with skin diseases. Due to the confusion and a lack of knowledge about their mental health issues, mental health services are often underutilized.
摘要：
BACKGROUND: Studies are ongoing to examine the versatile functions of circular RNAs (circRNAs) in human diseases. This research investigates the effects of hsa_circ_0000644 (circ_644) and its related molecules on the malignant behavior of bladder cancer (BCa) cells. METHODS: Abundant bioinformatics analyses were performed to screen the key circRNA and its related molecules in BCa. Tumor tissues and the para-tumorous tissues were collected from 58 patients with BCa. Expression of RUNX family transcription factor 3 (RUNX3), circ_644, microRNA-143-3p (miR-143-3p), and musashi RNA binding protein 2 (MSI2) in BCa tissues or cells was determined. Molecular interactions were confirmed by chromatin immunoprecipitation, RNA pull-down, and luciferase assays. Gain and loss-of function assays were performed using two BCa cell lines (T24 and HT1376). RESULTS: Circ_644 was highly expressed whereas RUNX3, which could suppress circ_644 transcription, was lowly expressed in BCa tissues and cells. Upregulation of RUNX3 suppressed proliferation, colony formation, migration and invasion, and tumorigenicity of BCa cells and induced cell cycle arrest. However, the tumor-suppressive effects of RUNX3 were blocked by circ_644 upregulation. Circ_644 served as a sponge for miR-143-3p, and miR-143-3p bound to MSI2 mRNA. The rescue experiments showed that miR-143-3p inhibition or MSI2 overexpression restored the malignant behaviors of BCa cells induced by circ_644 knockdown or RUNX3 overexpression. CONCLUSION: This study demonstrates that transcriptional activation of circ_644 upon RUNX3 downregulation drives the malignant development of BCa through the miR-143-3p/MSI2 axis. RUNX3 restoration or specific inhibition of circ_644 or MSI2 may help block BCa progression.
期刊：
REVISTA INTERNACIONAL DE MEDICINA Y CIENCIAS DE LA ACTIVIDAD FISICA Y DEL DEPORTE,2023年22(88):1-11 ISSN：1577-0354
通讯作者：
Liu, YH
作者机构：
[Li, Wenjuan; Qin, Zuoai; Liu, Nian; Fu, Zhongying; Yan, Jie; Ye, Haimin; Guo, Xiajun; Liu, Yehui; Wang, Xiaoyi; Zhou, Liu] Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, 233 Caie North Rd, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Liu, YH ] H;Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, 233 Caie North Rd, Changsha 410000, Hunan, Peoples R China.
关键词：
Neck acupuncture;Scalp acupuncture;Stroke;Cognitive impairment;Quality of life;Therapeutic effect
摘要：
Objective: To explore the intervention value of needle acupuncture and scalp acupuncture in improving cognitive impairment and life in stroke patients; Methods: A total of 62 stroke patients who were healed in our hospital from August 2019 to October 2021 were retrospectively selected as the research objects, and were divided into a combined healing cluster (Combined healing cluster, CTG, n=31, The patients received conventional healing combined with acupuncture and acupuncture) and the general healing cluster (GTG, n=31). The healing effects of the two clusters were contrast, and the National Institutes of Health Stroke Scale (NIHSS), neurological deficit score before and after healing Table (NDS) and Barthel Index (BI) score changes, the follow-up outcomes of the two clusters of patients were calculated and contrast between the two clusters; Results: (1) The total effective rate of patients in CTG cluster was 96.77%, and the total effective rate of patients in GTG cluster was 80.65%, and the variation in effective rate between the two clusters was notable (P<0.05). The NIHSS and NDS marks of the CTG cluster were notably bottom than those of the GTG cluster, and the variation between the clusters was notable (P<0.05). (3) On the 7th, 15th and 30th days of healing, the BI marks of the CTG cluster were notably upper than those in the GTG cluster, and the variation between the clusters was notable (P<0.05). (4) There were a total of 3 recurrences in the CTG cluster after 6 months of follow-up, with a recurrence rate of 10.00%, and a total of 9 recurrences in the GTG cluster. The recurrence rate of patients in the CTG cluster was notably bottom than that in the GTG cluster (P<0.05); Conclusion: The combined use of acupuncture and scalp acupuncture for stroke patients can help to enhance the healing effect and life of patients, enhance the neurological function of patients, and reduce the recurrence rate of stroke in patients in the short-term. It is recommended to popularize and apply it.
