作者机构：
[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.;[Li, Hua; Zhang, Guo-Min; Chen, Bei-Yang] Hunan Univ Tradit Chinese Med, Dept Pathol, Changsha, Hunan, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.;[Tang, Ying-Hong] Hunan Univ Tradit Chinese Med, Dept Pharmacol, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of "panax notoginseng root";Atorvastatin;Vascular smooth muscle cell;Proliferating cell nuclear antigen;Cyclind D-1;Cycline;Extracellular matrix;Collagen I;Fibronectin;Matrix metalloproteinase-9;Tissue inhibitor metalloproteinase-1
摘要：
Aim of the study: the effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, TSPN and atorvastatin group. Rat aorta intima in all groups were injured by insertion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proliferating cell nuclear antigen(PCNA), cyclinD1, cyclinE, collagen I(Col-I), fibronect(FN), matrix metalloproteinase-9(MMP-9) and tissue inhibitor metalloproteinase-1(TIMP-1). Results: TPNS significantly inhibited the vascular intimal hyperplasia. TPNS significantly lowered the expression of PCNA, cyclinE, cyclinD1, FN and MMP-9. TPNS had no significant impacts on the expression of Col-I and TIMP-1. Conclusions: Our studies indicated that TSPN could inhibit vessel restenosis after vascular intimal injury, and its mechanisms may be related to the blockage of the excessive proliferation of VSMC, the reduction of ECM protein deposition in the endometrium, and the degradation of ECM protein. (C) 2009 Elsevier GmbH. All rights reserved.
作者机构：
[Rao, Jian-Ming] Department of Urology, Hunan Traditional Chinese Medical University, Changsha, Hunan Province, China
通讯机构：
[Yang, Jin-Rui] Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.
摘要：
OBJECTIVE To prospectively evaluate perioperative results and 12-month follow-up after plasmakinetic enucleation of the prostate (PKEP) and transvesical open prostatectomy (OP) for benign prostatic hyperplasia (BPH) > 80 mL. METHODS A total of 83 patients with a prostate > 80 mL were randomized to either PKEP or OP. Perioperative and postoperative outcome data were obtained during a 12-month follow-up. RESULTS No statistical differences were observed in the preoperative data. Both groups resulted in a similar and significant postoperative improvement in International Prostate Symptom Score (IPSS), quality of life (QOL), maximum uroflow rate (Qmax), postvoid residual (PVR) urine volume and prostate specific antigen (PSA), but no significant difference was found between the groups at the 12-month follow-up. Compared to OP, operation time (111.2 +/- 27.1 minutes vs 109.6 +/- 28.2 minutes, P = .708) were not significantly different between the groups, but blood loss was significantly less (10.2 +/- 4.5 g/l vs 15.1 +/- 4.3 g/l, P < .001), and bladder irrigation (2.4 +/- 1.0 days vs 4.3 +/- 1.1 days, P < .001), catheterization time (3.3 +/- 1.1 days vs 6.2 +/- 1.3 days, P < .001), and hospital stay (5.4 +/- 1.2 days vs 9.3 +/- 1.1 days, P < .001) were significantly shorter in the PKEP group. Effects on erectile function were similar in both groups, but adverse events were less frequent in the PKEP group. CONCLUSION PKEP can be performed safely and is an equally effective procedure for treatment of large BPH with OP, with minimal complications and faster postoperative recovery. The PKEP helps to reduce the morbidity associated with OP and may become the attractive alternative to OP for patients with large BPH. UROLOGY 82: 176-181, 2013. (C) 2013 Elsevier Inc.
摘要：
Background: Relationships of some types of childhood maltreatment and suicidal behavior remain controversial and inconclusive. Methods: Medline, Embase, PsycINFO and Cochrane library were searched for eligible studies, and the results were synthesized in meta-analyses. Results: childhood maltreatment was associated positively with suicidal behavior in the total population and maltreatment subgroups. Emotional abuse had the strongest effect (OR = 2.33, SMD = 0.660, P < 0.001). Subgroup analyses showed that the effects of childhood abuse (OR = 1.55, SMD = 0.523) and neglect (OR = 1.25, SMD = 0.31) were significant. According to the analysis of dichotomous outcomes, childhood maltreatment was associated positively with suicidal behavior in men and women (women: OR = 4.84, P < 0.001; men: OR = 1.03, P < 0.001). Among populations, childhood maltreatment had the strongest effect on suicidal behavior in the general population (OR = 3.78, P < 0.001). However, the analysis of continuous outcomes showed that the effect was strongest in patients with chronic schizophrenia (SMD = 0.89, P < 0.001). In addition, childhood maltreatment was associated positively with suicide attempt (OR = 1.11, SMD = 0.48, P < 0.001), but not with suicidal ideation. Limitations: Some subgroup samples were not sufficiently large. Conclusions: Childhood maltreatment increases the risk of suicidal behavior. Emotional abuse had the strongest effect among the five types of maltreatment. The risk of suicidal behavior is higher in the general population, women, and individuals with chronic schizophrenia who have histories of childhood maltreatment.
作者机构：
[Liu, Jianbo; He, Yuqiong; Xiao, Bo; Shen, Yanmei; Luo, Xuerong] 湖南中医药大学，Cent S Univ, Xiangya Hosp 2, Mental Hlth Inst, Changsha 410000, Hunan, Peoples R China.;[Liu, Jianbo; He, Yuqiong; Xiao, Bo; Shen, Yanmei; Luo, Xuerong; Luo, XR (reprint author)] Cent S Univ, Key Lab Psychiat & Mental Hlth Hunan Prov, Changsha 410000, Hunan, Peoples R China.;[Gong, Jingbo] Tradit Chinese Med Univ Hunan, Dept Appl Psychol, Changsha 410208, Hunan, Peoples R China.;[Nie, Guanghui] Guangxi Med Univ, Sch Publ Hlth, Nanning 530000, Peoples R China.;[Luo, XR (reprint author)] Cent S Univ, Xiangya Hosp 2, Mental Hlth Inst, Changsha 410000, Hunan, Peoples R China.
通讯机构：
[Luo, Xuerong] Cent S Univ, Xiangya Hosp 2, Mental Hlth Inst, Changsha 410000, Hunan, Peoples R China.;[Luo, Xuerong] Cent S Univ, Key Lab Psychiat & Mental Hlth Hunan Prov, Changsha 410000, Hunan, Peoples R China.
关键词：
APT;SPT;Neglect;Depression;Mediating factor
摘要：
Background: Autistic personality traits (APT) and schizotypal personality traits (SPT) are associated with depression. However, mediating factors within these relationships have not yet been explored. Thus, the focus of the current study was to examine the effects of childhood neglect on the relationship between APT/SPT and depression. Methods: This cross-sectional study was conducted on first-year students (N = 2469) at Hunan University of Chinese Medicine and Hengyang Normal College (Changsha, China). Participants completed surveys on APT, SPT, childhood neglect, abuse and depression. Results: Through correlational analyses, APT and SPT traits were positively correlated with childhood neglect and depression (p < 0.05). In a hierarchical regression analysis, among types of childhood maltreatment, emotional neglect (beta = 0.112, p < 0.001) and physical neglect (beta = 0.105, p < 0.001) were the strongest predictors of depression. Childhood neglect did not account for the relationships between APT/SPT and depression. Further analysis found that childhood neglect mediated the relationship between SPT and depression but not APT and depression. Conclusions: Among types of childhood maltreatment, neglect was the strongest predicting factor for depression. Neglect did not account for the relationship between APT/SPT and depression but was a strong mediating factor between SPT and depression.
期刊：
SOUTH AFRICAN JOURNAL OF PSYCHIATRY,2017年23 ISSN：1608-9685
通讯作者：
Luo, Xuerong
作者机构：
[Xiao, Bo; Liu, Jianbo; Luo, Xuerong] 湖南中医药大学，Cent S Univ, Mental Hlth Inst, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.;[Xiao, Bo; Liu, Jianbo; Luo, Xuerong; Luo, XR (reprint author)] China Natl Clin Res Ctr Mental Hlth Disorders, Natl Technol Inst Psychiat, Key Lab Psychiat & Mental Hlth Hunan Prov, Hengyang, Peoples R China.;[Gong, Jingbo] Tradit Chinese Med Univ Hunan, Dept Appl Psychol, Changsha, Hunan, Peoples R China.;[Luo, XR (reprint author)] Cent S Univ, Mental Hlth Inst, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Luo, Xuerong] Cent S Univ, Mental Hlth Inst, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.;[Luo, Xuerong] China Natl Clin Res Ctr Mental Hlth Disorders, Natl Technol Inst Psychiat, Key Lab Psychiat & Mental Hlth Hunan Prov, Hengyang, Peoples R China.
摘要：
Objective: This study assessed the mediating role of perceived parental rejection in the relationship between childhood maltreatment experience and behavioural problems in Chinese adolescents. Methods: A total of 2484 adolescents (1305 males and 1179 females; aged 12-16 years) from Hunan Province, China, participated in the study. Behavioural problems, parental rejection scores and child abuse experiences were evaluated by the Child Behavior Checklist (parental version), the Memories of Parental Rearing Behavior Scale and the Childhood Trauma Questionnaire, separately. Mediating effects were examined by structural equation modelling using Amos 20 software. Results: The study found that perceived maternal rejection partially mediated the association between abuse and internalising behaviours in the male cohort, whereas perceived father's rejection partially mediated this association in the female cohort. However, mental illness had no moderating effect on these relationships. Conclusion: These results are consistent with the literature on maltreatment and parent-child relationships and provide empirical support for the view that emotional and behavioural problems related to perceived parental rejection underlie the development of psychosocial problems in adolescents.
作者机构：
[Deng, Chang-Qing; Chen, Gang] Hunan Univ Tradit Chinese Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Pathophysiol Lab, Shaoshan Rd 113, Changsha, Hunan, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Pathophysiol Lab, Shaoshan Rd 113, Changsha, Hunan, Peoples R China.
关键词：
BuYang HuanWu Decoction;Vascular smooth muscle cell;Platelet derived growth factor;Proliferating cell nuclear antigen;c-fos;CyclinD(1);Extracellular regulated protein kinases1/2;Mitogen-activated protein kinase;phosphatase-1
摘要：
Buyang Huanwu Decoction (BYHWD) was a commonly used traditional Chinese medicine for the treatment and prevention of ischemic cardiovascular and cerebral disease. Previous studies had shown that BYHWD alkaloids and glycosides could inhibit intimal hyperplasia and vascular smooth muscle cell (VSMC) proliferation after injury caused by balloon catheter. The present study aims to explore the mechanisms by which cell cycle was affected by BYHWD and its components. Primary rat VSMC was treated with platelet-derived growth factor (PDGF) and cell cycle phase and extracellular-signal regulated protein kinase (ERK) transduction pathway factors were measured. PDGF-treated cells were associated with a significant increase in the number of cells in the G(2)/M phase and S phase, and in the expression of P-ERK1/2, proliferating cell nuclear antigen (PCNA), c-fos, cyclinD(1) and cyclin-dependent kinase-4, as well as a decrease in the number of cells in the G(0)/C(1) phase, and in the expression of cyclin-dependent kinase inhibitor P21 protein and mitogen-activated protein kinase phosphatase-1 (MKP-1). Treatment with plasma of rats fed seven doses of BYHWD crude extract (22.2 g/kg), BYHWD alkaloids (1.66 g/kg), BYHWD glycosides (14.2 g/kg) or the negative control atorvastatin (20 mg/kg) inhibited these changes. All drug-containing plasma had similar activity to the mitogen activated protein kinase (MAPK)/ERK antagonist PD098059 which inhibited PDGF-induced expression of P-ERK1/2 and enhanced MKP-1. These suggest that BYHWD and its components may prevent VSMC proliferation by interfering with the ERK transduction pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
作者：
Peng, Tian-Shu;He, Yong-Heng*;Nie, Tian;Hu, Xiang-Dang;Lu, Hai-Yan;Yi, Jian;Shuai, Yun-Fei;Luo, Min
期刊：
Experimental and therapeutic medicine,2014年8(2):430-434 ISSN：1792-0981
通讯作者：
He, Yong-Heng
作者机构：
[Peng, Tian-Shu; He, Yong-Heng; Hu, Xiang-Dang; Lu, Hai-Yan; Luo, Min] Hunan Univ Chinese Med, Dept Anorectal Dis, Affiliated Hosp 2, Changsha 410005, Hunan, Peoples R China.;[He, Yong-Heng] Hunan Univ Chinese Med, Dept Anorectal Dis, Affiliated Hosp 2, 233 Caie North Rd, Changsha 410005, Hunan, Peoples R China.;[Nie, Tian; Yi, Jian; Shuai, Yun-Fei] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Blood & Oncol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[He, Yong-Heng] Hunan Univ Chinese Med, Dept Anorectal Dis, Affiliated Hosp 2, 233 Caie North Rd, Changsha 410005, Hunan, Peoples R China.
关键词：
colorectal cancer;PPM1D;prognosis;biomarker
摘要：
Protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D) has been associated with carcinogenesis. The present study investigated PPM1D expression as a potential biomarker in colorectal cancer (CRC). PPM1D expression was assessed using immunohistochemistry in 368 patients with CRC. The correlation between PPM1D expression, clinicopathological features and prognosis was analyzed. PPM1D small interfering (si)RNA-induced PPM1D silencing was performed in CRC cell lines to assess the effect of PPM1D on tumor cell proliferation and invasion in vitro. A total of 68.48% (252/368) of the CRC samples displayed high PPM1D expression. By contrast, only 9.24% (34/368) of the matched non-cancerous tissue samples exhibited high PPM1D expression. High PPM1D expression was correlated with node metastasis (P=0.0024), distant metastasis (P<0.001) and TNM stage (P=0.0016). Kaplan-Meier survival analysis revealed that patients with low PPM1D expression had significantly longer survival than those with high PPM1D expression (P=0.012). Moreover, multivariate analyses demonstrated that high PPM1D expression was an independent prognostic factor for overall survival (hazard ratio = 0.24; 95% confidence interval, 0.13-0.86; P=0.004). Furthermore, PPM1D gene silencing was found to significantly reduce the proliferation and invasion of CRC cells in vitro. These findings suggest a role for PPM1D as a prognostic marker and potential therapeutic target in CRC.
摘要：
Aim of the study: The inhibitive effect of BuYang HuanWu Decoction (BYHWD) and its major components on vascular intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix protein. Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, alkaloid, glycoside, BYHWD and atorvastatin groups. Rat aorta intima in all groups were injured by insesion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proteins in vascular cells associated with cell cycle including proliferating cell nuclear antigen(PCNA), cyclinD(1) and cyclinE, and extracellular matrix(ECM) proteins including collagen I (Col-1) and fibronectin (FN), further to discover the involved biologically active substances and the potential mechanisms. Results: Alkaloid and glycosid isolated from BYHWD were more effective than BYHVVD in the inhibition of intimal hyperplasia and the expressions of PCNA, cyclinD(1), cychnE, Col-I and FN, suggesting that alkaloid and glycoside may be the main components of BYHWD responsible for the observed inhibition of excessive hyperplasia of vascular intima. Conclusions: The mechanism associated with the anti-hyperplasia activity of BYHVVD and its effective components may be related to the blockage of cell cycles of VSMC, and the inhibition of the ECM protein synthesis, even the increased degradation of ECM proteins. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
作者机构：
[Tang, Jintian] Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing, 100084, China;[Cheng, Yan] School of Pharmacy, Central South University, Changsha, Hunan 410013, China;[Xie, Huiqing] Department of Rehabilitation, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China;[Xie, Biao; Zhou, Jianda; Chen, Jia] Deptment of Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, China;[Xie, Biao] Department of Colorectal Surgery, Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, Hunan 410005, China
通讯机构：
[Zhou, Jianda] Cent S Univ, Xiangya Hosp 3, Dept Plast Surg, Changsha 410013, Hunan, Peoples R China.;[Xie, Huiqing] Cent S Univ, Xiangya Hosp 3, Dept Rehabil, Changsha 410013, Hunan, Peoples R China.
摘要：
The molecular and cellular mechanisms behind the involvement of inflammation in melanoma have not been fully elucidated. In this study, knockdown of Hmgb1 expression increased apoptosis, reduced invasion and p-NF-kappa B expression, but increased Klotho protein level in melanoma tumor cells. The effect of Hmgb1 knockdown was overcome by LPS. Introduction of exogenous Hmgb1 significantly decreased apoptosis, increased invasion, elevated p-NF-kappa B, but lowered Klotho protein level in melanoma cells. The effect of exogenous Hmgb1 was agonized by NF-kappa B inhibitor CAPE. Hmgb1 knockdown activated, but exogenous Hmgb1 inactivated, p-IGF1R/p-PI3K p-85/p-Akt/p-mTOR signaling. Knockdown of Klotho gene expression significantly decreased apoptosis, increased invasion in melanoma cells, and inhibited xenograft A375 tumor growth. A significantly high percentage of cells stained positive for p-NF-kappa B, but negative for Klotho, in melanoma tissues compared to normal and benign skin tissues. The positive p-NF-kappa B and negative Klotho protein expression correlated with poor prognosis in melanoma patients. Multivariate analysis revealed an independent association between p-NF-kappa B / Klotho protein level and overall survival. In conclusion, Hmgb1 can inhibit Klotho gene expression and malignant phenotype in melanoma cells through activation of NF-kappa B signaling.
作者：
Su, Feng;Zhu, Shilin;Ruan, Jinlan;Muftuoglu, Yagmur;Zhang, Longbo*;Yuan, Qianying*
期刊：
Oncotarget,2016年7(4):4142-4154 ISSN：1949-2553
通讯作者：
Zhang, Longbo;Yuan, Qianying
作者机构：
[Muftuoglu, Yagmur; Yuan, Qianying] Department of Pharmacology, Yale University School of Medicine, New Haven, CT, USA;[Yuan, Qianying; Ruan, Jinlan] Department of Pharmacology, Huazhong University of Science and Technology, Wuhan, China;[Zhu, Shilin] The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China;[Su, Feng] Department of Emergency, Xiangya Hospital, Central South University, Changsha, China;[Zhang, Longbo] Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, China
通讯机构：
[Zhang, Longbo] Cent S Univ, Xiangya Hosp, Dept Neurosurg, Changsha, Hunan, Peoples R China.;[Yuan, Qianying] Huazhong Univ Sci & Technol, Dept Pharmacol, Wuhan 430074, Peoples R China.;[Yuan, Qianying] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06510 USA.
关键词：
RY10-4;resistance;Notch;breast cancer
摘要：
RY10-4, a novel protoapigenone analog, shows potent cytotoxicity against human breast cancer cells. However, breast cancer cell lines overexpressing human epidermal growth factor receptor 2 (HER2), SKBR3 and BT474, showed less sensitivity to RY10-4 when compared to breast cancer cells lines expressing lower levels of HER2, such as MDA-MB-231 and MCF-7 cells. This was associated with aberrant hyperactivity in Notch signaling in cells treated with RY10-4, since treatment with RY10-4 causes an increase in Notch activity by 2-to3.5-fold in SKBR3 and BT474 cell lines. The increase in activity was abrogated with a gamma-secretase inhibitor, DAPT, or with Notch1 small-interfering RNA (si-Notch1). Cell proliferation was inhibited more effectively by RY10-4 plus DAPT or si-Notch1 than either agent alone. RY10-4 plus DAPT increases apoptosis in both HER2-overexpressing cell lines by two-fold compared to RY10-4 alone, while DAPT alone has no significant effects on apoptosis. In addition, we previously found RY10-4 could inhibit tumor growth through the PI3K/AKT pathway. Here we report that the combination of RY10-4 and DAPT exhibit additive suppression on AKT phosphorylation, contributing to the anti-cancer effects. In an animal model, this combination therapy inhibits the growth of SKBR3 tumor xenografts in nude mice to a greater extent than treatment with either reagent alone. These results indicate that the aberrant activation of Notch signaling impedes the inhibitory effect of RY10-4 on HER2-amplified cell proliferation. Furthermore, these adverse effects can be prevented by treatment combining RY10-4 with a Notch pathway inhibitor.
作者机构：
[Huang, Pan; Wang, Chang; Wang, Haizhen; Yang, Zhibo; Pan, Yi] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province 410005, China;[Zeng, Bijun] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province 410005, China. Electronic address: dr_zengbijun@163.com
通讯机构：
[Zeng, Bijun] 233 Caie North Rd, Changsha 410005, Hunan, Peoples R China.
关键词：
MicroRNA 124;Atopic eczema;Inflammation;NF-kappa B (p65);Keratinocytes
摘要：
Chronic skin inflammation in atopic eczema is associated with elevated expression of proinflammatory genes and activation of innate immune responses in keratinocytes. MicroRNAs (miRNAs) are short, single-stranded RNA molecules that silence genes via the degradation of target mRNAs or inhibition of translation. Recent studies have demonstrated that miR-124 is associated with regulation of inflammation factors in several diseases. The aim of this study was to investigate the role of miR-124 in skin inflammation of atopic eczema. We showed that miR-124 expression is decreased in chronic lesional skin of patients with atopic eczema, and could be strongly inhibited by IFN-gamma and TNF-alpha. Through Western blot, real-time PCR and luciferase assays, we revealed that miR-124 inhibited the expression of p65, a member of NF-kappaB family which can regulate many factors involved in the immune response and inflammatory reactions, through direct targeting. Further, upon IFN-gamma or TNF-alpha stimulation, IL8, CCL5 and CCL8 showed to be significantly upregulated by IFN-gamma or TNF-alpha, downregulated by miR-124; the promotive effect of IFN-gamma and TNF-alpha could be partially reversed by miR-124. The levels of IL8, CCL5 and CCL8 could be significantly downregulated by p65 knockdown, upregulated by miR-124 inhibition; the suppressive effect of p65 knockdown could be partially reversed by miR-124. Moreover, contrary to miR-124, p65, IL8, CCL5 and CCL8 mRNA expression was upregulated in chronic lesional skin of patients with atopic eczema, and all inversely correlated with miR-124. Taken together, our data demonstrate that miR-124 controls NF-kappaB-dependent inflammatory responses in keratinocytes and chronic skin inflammation in atopic eczema; rescuing miR-124 expression presents a promising strategy for atopic eczema treatment.
作者机构：
[Zhao, Shuang; Su, Juan; Li, Fangfang; Chen, Xiang] Department of Dermatology, Xiangya Hospital, Central South University, Changsha, Hunan, China;[Zhao, Shuang; Su, Juan; Li, Fangfang; Chen, Xiang] Hunan Key Laboratory of Skin Cancer and Psoriasis, Xiangya Hospital, Central South University, Changsha, Hunan, China;[Zhu, Shilin] Department of Neurology, The Second Affiliated Hospital of Hunan University of TCM, Changsha, Hunan, China;[Wu, Lisa] Institute of Medical Science Research, Xiangya Hospital, Central South University, Hunan, China;[Su, Feng] Department of Emergency, Xiangya Hospital, Central South University, Changsha, China
通讯机构：
[Su, Juan] Cent S Univ, Xiangya Hosp, Dept Dermatol, Changsha, Hunan, Peoples R China.;[Su, Juan] Cent S Univ, Xiangya Hosp, Hunan Key Lab Skin Canc & Psoriasis, Changsha, Hunan, Peoples R China.
摘要：
Malignant melanoma is the most aggressive type of skin cancer. RAB22A, a member of RAS oncogene family, has been found to be significantly upregulated in multiple human cancers. In the present study, we found that RAB22A mRNA expression was significantly upregulated in melanoma tissues (including 60 primary melanomas and 84 metastatic melanomas) compared to benign nevi (n = 20), which were significantly higher in metastatic melanoma tissues than primary tissues. Immunohistochemistry data further showed that the positive immunoreactivity of RAB22A was detected in 66% (95/144) melanoma tissues, but not in benign nevi. Moreover, high expression of RAB22A was significantly associated with advanced clinical stage in melanoma. Furthermore, patients with high RAB22A expression had shorter overall survival compared those with low expression of RAB22A. In-vitro study showed that RAB22A was also upregulated in melanoma cell lines WM35, A375, WM451, and SK-MEL-1, when compared with the normal melanocyte HM cells. Knockdown of RAB22A significantly reduced the proliferation, migration and invasion of melanoma A375 cells, while overexpression of RAB22A significantly promoted these malignant phenotypes. In addition, RAB22A was found to be a target of miR-203, a tumor suppressive miRNA in melanoma. Besides, miR-203 was downregulated in melanoma tissues and cell lines, when compared with benign nevi and HM cells, respectively. Taken these findings together, our study could validate an oncogenic role of RAB22A in melanoma, suggesting that RAB22A may be a potential therapeutic target for melanoma.
期刊：
MOLECULAR MEDICINE REPORTS,2017年17(1):660-666 ISSN：1791-2997
通讯作者：
Tang, Siyuan;Su, Feng
作者机构：
[Zhu, Shilin] Department of Neurology, The Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, Hunan 410005, P.R. China;[Tang, Siyuan] Xiang Ya Nursing School of Central South University, Changsha, Hunan 410013, P.R. China;[Su, Feng] Department of Emergency, Xiang Ya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
通讯机构：
[Tang, Siyuan] Cent S Univ, Xiang Ya Nursing Sch, 172 Tongzipo Rd, Changsha 410013, Hunan, Peoples R China.;[Su, Feng] Cent S Univ, Xiang Ya Hosp, Dept Emergency, 87 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China.
摘要：
Diosgenin, as an essential natural steroidal saponin, can be extracted from numerous sources, primarily from fenugreek. It is an important raw material for the synthesis of steroid hormone drugs. It exhibits antitumor, antiinflammatory, antioxidation and several other significant pharmacologic actions, and is of high pharmaceutical value. In the present study, the activities and underlying mechanisms of dioscin in the inhibition of ischemic stroke in rats were investigated. Inflammatory responses wer analyzed using ELISA kits and caspase3 and caspase9 activity was analyzed using Caspase3 and caspase9 activity kits. Western blot analysis was used to measure Tolllike receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factorkappaB (NFkappaB), transforming growth factorbeta1 (TGFbeta1), highmobility group protein 1 (HMGB1), interleukin1 receptorassociated kinase 1 (IRAK1), and tumor necrosis factor receptorassociated factor 6 (TRAF6) protein expression. Dioscin inhibited infarct volume and neurological scores in the ischemic stroke rat model. The results demonstrated that dioscin reduced inflammatory responses, and suppressed the expression of TLR4, MyD88, NFkappaB, TGFbeta1, HMGB1, IRAK1, and TRAF6 in the rat ischemic stroke model. Taken together, these findings suggested that dioscin inhibited ischemic strokeinduced inflammation through inhibition of the TLR4/MyD88/NFkBinduced inflammation the rat model, which provided novel insights into the mechanisms underlying the effect of dioscin as an antiinflammatory candidate for the treatment of ischemic stroke in in the future.
摘要：
Introduction: Psoriasis is a common and recurrent disease and difficult to treat. The aim was to develop an evidence-based clinical practice guideline of Chinese herbal medicine (CHM) for psoriasis vulgaris with focus on Chinese medicine pattern differentiation and treatment as well as approved herbal proprietary medicine. Methods: The guideline development group involved in various expertise in contents and methods. Authors searched electronic databases include CNKI, VIP, Sino-Med, Wanfang data, PubMed, the Cochrane Library, EMBASE, as well as checked China State Food and Drug Administration (SFDA) from the inception till March 31, 2013. Systematic reviews and randomized controlled trials of Chinese herbal medicine treating adults with psoriasis vulgaris were evaluated. Risk of bias tool in the Cochrane Handbook and evidence strength developed by the GRADE group were applied for the evaluation, and recommendations were based on the findings incorporating evidence strength. After several rounds of expert consensus, the final guideline was endorsed by relevant professional committees. Results: CHM treatment principle and formulae based on pattern differentiation together with approved patent herbal medicines are the main treatments for psoriasis vulgaris, and the diagnosis and treatment focusing on blood related patterns is the major domain. Conclusion: CHM therapy alone or combined with other conventional treatment reported in clinical studies together with expert consensus were recommended for clinical practice. (C) 2014 Elsevier GmbH. All rights reserved.
摘要：
Numb, an endocytic adaptor, is a known cell fate determinant that participates in asymmetric cell division. The present study aimed to explore the potential roles of Numb in hepatocarcinogenesis. Numb expression was investigated in hepatocellular carcinomas (HCC) with reverse transcription-quantitative polymerase chain reaction and immunohistochemical examination; its association with the prognosis of HCC patients was analyzed. In addition, the effects of Numb deletion on proliferation of HCC cells and its relevant molecules were evaluated in Huh7 and HepG2 cells. Numb overexpression was observed in 62% of adjacent non-tumor tissues and 46% of tumor tissues. Overexpression of Numb in HCC was associated with histological grade, portal vein invasion and the number of tumors (P=0.001, 0.022 and 0.034 respectively). Multivariate analysis revealed that Numb expression was an independent prognostic indicator of HCC patients. Methylation of the Numb promoter contributed to hepatocarcinogenesis. In vitro assays demonstrated that Numb silencing resulted in inhibition of cell proliferation, induction of apoptosis, down-regulation of cyclin-dependent protein kinase 4 (CDK4) and S-phase kinase-associated protein 2 (SKP2), and upregulation of Bcl-2 homologous antagonist/killer (BAK) and cyclin-dependent kinase inhibitor 1 (p21). The present study suggests that downregulation of Numb inhibits colony formation and cell proliferation, induces apoptosis of HCC cells and independently predicts the poor prognosis of HCC patients. Thus, Numb has a potential role in the development and progression of HCC.
作者机构：
[Zeng, Bijun; Wang, Chang; Tang, Xueyong; Wang, Haizhen; Shen, Hui; Liu, Wen] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, 410005, China;[Shen, Hui] Department of Dermatology, Ruikang Hospital affiliated to Guanxi University of Chinese Medicine, Nanning, Guangxi Province, 530000, China;[Yang, Zhibo] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan Province, 410005, China. Electronic address: dr_yangzhibo@163.com
通讯机构：
[Yang, Zhibo] 233 Caie North Rd, Changsha 410005, Hunan, Peoples R China.
关键词：
MiR-330;IL-22;CTNNB1;Keratinocyte;Psoriasis
摘要：
Psoriasis is a common chronic inflammatory skin disease which is characterized by hyperproliferation and aberrant differentiation of keratinocytes; however the exact pathogenesis is largely unknown. Interleukin-22 (IL-22) has demonstrated its vital role in T cell-mediated immune response by interacting with keratinocytes in the pathogenesis of psoriasis. The microRNAs (miRNAs) are a class of small noncoding RNA molecules that play important roles in cellular processes by regulating gene expression at the post-transcriptional level. MiR-330 has been reported to inhibit the proliferation and migration of mouse keratinocytes. In the present study, we indicated that miR-330 expression in lesion tissue of psoriasis patients was specifically down-regulated, and could inhibit IL-22-induced proliferation of HaCaT and HKC cell. Wnt/beta-catenin pathway plays an essential role in the pathogenesis of psoriasis. By direct targeting CTNNB1, miR-330 could significantly downregulate IL-22-induced CTNNB1 expression. In addition, we found that the downstream targets of b-catenin, CyclinD1 and Axin2, could be affected by miR-330; miR-330 could suppress CyclinD1 protein expression and rescue Axin2 protein expression. Taken together, we indicated miR-330 inhibits IL-22-induced proliferation of HaCaT and HKC cell by targeting CTNNB1 and subsequently affect the downstream factors, CyclinD1 and Axin2 for the first time, and provide diagnostic markers and a novel target for psoriasis treatment. (C) 2017 Published by Elsevier Masson SAS.
摘要：
The beta 1-adrenergic receptor (AR) is the primary beta-AR subtype in the heart and is the target of metoprolol (Met), which is commonly used to treat angina and hypertension. Previous studies have revealed a positive correlation between the methylation levels of the adrenoreceptor beta 1 gene (Adrb1) promoter in the myocardium with the antihypertensive activity of Met in spontaneously hypertensive rats (SHR), which affects beta 1-AR expression in H9C2 cells. The aim of the present study was to investigate the effects of myocardial beta 1-AR downregulation using short-hairpin RNA (shRNA) against Adrb1 on the antihypertensive activity of Met in SHR. Recombinant adeno-associated virus type 9 (rAAV9) vectors carrying Adrb1 shRNA (rAAV9-Adrbl) or a negative control sequence (rAAV9-NC) were generated and used to infect rat hearts via the pericardial cavity. The results of reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting analyses demonstrated that cardiac beta 1-AR expression in the rAAV9-Adrbl group was significantly downregulated when compared with the rAAV9-NC group (P<0.001, P<0.001 and P=0.032, respectively). In addition, a greater reduction in systolic blood pressure (SBP) was observed in the rAAV9-NC group compared with the rAAV9-Adrbl group following Met treatment (P=0.035). Furthermore, downregulation of myocardial beta 1-AR was associated with a significant decrease in SBP (P<0.001). In conclusion, these data suggest that suppression of beta 1-AR expression in the myocardium reduces SBP and sensitivity to Met in SHR.
期刊：
INTERNATIONAL UROLOGY AND NEPHROLOGY,2014年46(11):2063-2070 ISSN：0301-1623
通讯作者：
Yang, Jin-Rui
作者机构：
[Ren, Yi-Xin; Ding, Ping; Yan, Yong-Li; Rao, Jian-Ming; Yang, Jing-Hua; He, Jiang] Hunan Tradit Chinese Med Univ, Dept Urol, Hunan Prov Peoples Hosp 2, Changsha 410007, Hunan, Peoples R China.;[Yang, Jin-Rui] Cent S Univ, Dept Urol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Xiao, Heng-Jun] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Urol, Guangzhou 510630, Guangdong, Peoples R China.
通讯机构：
[Yang, Jin-Rui] Cent S Univ, Dept Urol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.
关键词：
Benign prostatic hyperplasia;Plasmakinetic enucleation of the prostate;Prostate size;Therapy
摘要：
OBJECTIVE: To evaluate surgical complications and outcomes based on prostate size in patients with benign prostatic hyperplasia (BPH) treated with plasmakinetic enucleation of the prostate (PKEP). METHODS: A retrospective review was conducted of PKEP performed between July 2008 and January 2013. According to the prostate size on preoperative transrectal ultrasonography measurement, patients were divided into three groups: group 1: <40 ml, group 2: 40-80 ml and group 3: >80 ml. Baseline, perioperative and postoperative data were obtained. RESULTS: There were significant differences among the three groups regarding the mean operative time (p < 0.001) and the mean resected tissue weight (p < 0.001). But enucleation efficiency (p < 0.001) in gm tissue per minute increased significantly as prostate size increased. Mean hemoglobin decrease (p > 0.05), mean postoperative irrigation time (p > 0.05), mean catheter time (p > 0.05) and mean hospital stay (p > 0.05) did not differ significantly among three groups. The three groups had a similar and significant postoperative improvement in International Prostate Symptom Score, quality of life, maximum uroflow rate and post-void residual urine volume independent of prostate size (p < 0.001), but no significant difference was found among three groups at the 12-month follow-up (p > 0.05). Perioperative and postoperative complications did not depend on prostate size (p > 0.05). CONCLUSIONS: Although patients with a larger BPH required significantly longer operation time in PKEP, prostate size did not affect perioperative and postoperative complications or micturition improvement.
作者机构：
[Yang, Zhibo; Pan, Yi; Huang, Pan; Wang, Chang] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan province, 410005, China;[Zeng, Biyun] Department of Dermatology, The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan province, 410005, China. Electronic address: dr_zengbijun@163.com
通讯机构：
[Zeng, Biyun] 233 Caie North Rd, Changsha 410005, Hunan, Peoples R China.
摘要：
Melanin is the pigment responsible for the color of human skin and hair. Melanin serves as a double-edge sword which can exert both protective and spot-causing effects on skin. Although melanin has an important role in protecting the skin against UV damage, an excessive or uneven melanin production can lead to the formation of freckles and age spots. Isoliquiritigenin (ISL) has been reported to inhibit melanin synthesis; however, its role in melanin degradation remains unclear. In the present study, we evaluated the detailed function of ISL in melanin degradation in human epidermal keratinocytes. Since autophagy has been reported to be related to melanin degradation, we also examined the activation of autophagy by ISL treatment in keratinocytes by measurement of autophagy-related proteins, ATG7, LC3 and p62. Moreover, si-ATG7-induced ATG7 knockdown and autophagy inhibitor 3-MA decreased LC3 II protein levels and increased PMEL17, p62 and melanin levels in HaCaT cells, which could be partially reversed by ISL treatment, indicating that autophagy participated in melanin degradation. The decreased p-AKT and p-mTOR proteins upon ISL treatment indicated the involvement of PI3K/AKT/mTOR signaling in ISL-induced melanin degradation. Taken together, we demonstrated that autophagy participates in ISL-induced melanin degradation in human epidermal keratinocytes through PI3K/AKT/mTOR signaling.
作者机构：
[He, Xiaojuan; Lu, Aiping; Niu, Xuyan; Tan, Yong; Lu, Cheng] Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China;[Lu, Aiping; Zhang, Ge; He, Bing; Bian, Zhaoxiang; Xu, Gang] Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China;[Lu, Aiping] E-Institutes of Shanghai Municipal Education Commission, Shanghai, China;[Zhou, Ke] Second Affiliated Hospital, Hunan University of Chinese Medicine, Changsha, China;[Liu, Xinru; Zhang, Weidong; Zu, Xianpeng] School of Pharmacy, Second Military Medical University, Shanghai, China
通讯机构：
[Zhang, Ge; Lu, Aiping] Hong Kong Baptist Univ, Sch Chinese Med, Inst Adv Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China.;[Zhang, Weidong] Second Mil Med Univ, Sch Pharm, Shanghai, Peoples R China.;[Lu, Aiping] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing, Peoples R China.;[Lu, Aiping] Shanghai Municipal Educ Commiss, E Inst, Shanghai, Peoples R China.
摘要：
Hyperuricemia (HU) often progresses to combine with non-alcoholic fatty liver disease (NAFLD) in the clinical scenario, which further exacerbates metabolic disorders; early detection of biomarkers, if obtained during the HU progression, may be beneficial for preventing its combination with NAFLD. This study aimed to decipher the biomarkers and mechanisms of the development of steatosis in HU. Four groups of subjects undergoing health screening, including healthy subjects, subjects with HU, subjects with HU combined with NAFLD (HU+NAFLD) and subjects with HU initially and then with HU+NAFLD one year later (HU-->HU+NAFLD), were recruited in this study. The metabolic profiles of all subjects' serum were analyzed by liquid chromatography quadruple time-of-flight mass spectrometry. The metabolomic data from subjects with HU and HU+NAFLD were compared, and the biomarkers for the progression from HU to HU+NAFLD were predicted. The metabolomic data from HU-->HU+NAFLD subjects were collected for further verification. The results showed that the progression was associated with disturbances of phospholipase metabolism, purine nucleotide degradation and Liver X receptor/retinoic X receptor activation as characterized by up-regulated phosphatidic acid, cholesterol ester (18:0) and down-regulated inosine. These metabolic alterations may be at least partially responsible for the development of steatosis in HU. This study provides a new paradigm for better understanding and further prevention of disease progression.
