期刊：
DNA and cell biology,2013年32(6):302-309 ISSN：1044-5498
通讯作者：
Peng, D
作者机构：
[Jin, Yi; Peng, Dan; Shen, Yi; Xu, Min] Cent S Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410000, Hunan, Peoples R China.;[Liang, Ying] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Changsha, Hunan, Peoples R China.;[Lu, Jing] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Xiao, Bowen] Cent Hosp Changsha, Thorac & Cardiovasc Surg Ward, Changsha, Hunan, Peoples R China.
通讯机构：
[Peng, D] Cent S Univ, Xiangya Hosp 2, Dept Orthoped, Changsha 410000, Hunan, Peoples R China.
摘要：
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-376c expression level was decreased while transforming growth factor-alpha (TGFA) mRNA expression levels were increased in osteosarcoma tissues and cell lines, and we identified TGFA as a novel direct target of miR-376c. Overexpression of miR-376c suppressed TGFA expression and the expression of its downstream signaling molecule such as epidermal growth factor receptor, and attenuated cell proliferation and invasion. Forced expression of TGFA could partly rescue the inhibitory effect of miR-376c in the cells. Taken together, these findings will shed light on the role and mechanism of miR-376c in regulating osteosarcoma cell growth via miR-376c/TGFA axis, and miR-376c may serve as a potential therapeutic target in osteosarcoma in the future.
摘要：
Bipolar disorder and unipolar depressive disorder (UD) may be different in brain structure. In the present study, we performed voxel-based morphometry (VBM) to quantify the grey matter volumes in 23 patients with bipolar I depressive disorder (BP1) and 23 patients with UD, and 23 age-, gender-, and education-matched healthy controls (HCs) using magnetic resonance imaging. We found that compared with the HC and UD groups, the BP1 group showed reduced grey matter volumes in the right inferior frontal gyrus and middle cingulate gyrus, while the UD group showed reduced volume in the right inferior frontal gyrus compared to HCs. In addition, correlation analyses revealed that the grey matter volumes of these regions were negatively correlated with the Hamilton depression rating scores. Taken together, the results of our study suggest that decreased grey matter volume of the right inferior frontal gyrus is a common abnormality in BP1 and UD, and decreased grey matter volume in the right middle cingulate gyrus may be specific to BP1.
作者机构：
[Zu, Xu-yu; Sun, Shao-wei; Tuo, Qin-hui; Lei, Xiao-yong; Liao, Duan-fang; Chen, Lin-xi] Univ S China, Inst Pharm & Pharmacol, Prov Key Lab Pharmacoprotom, Hengyang 421001, Peoples R China.;[Sun, Shao-wei; Liao, Duan-fang; Tang, Chao-ke] Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.;[Liao, Duan-fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Sch Pharm, Changsha 410208, Hunan, Peoples R China.;[Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215004, Peoples R China.;[Li, Kai] Suzhou Univ, Affiliated Hosp 2, Suzhou 215004, Peoples R China.
通讯机构：
[Tang, CK] Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
关键词：
caveolae;caveolin-1;oxidized low density lipoprotein;atherosclerosis;transcytosis;human umbilical vein endothelial cells
摘要：
Aim: To explore the mechanisms involved in ox-LDL transcytosis across endothelial cells and the role of caveolae in this process. Methods: An in vitro model was established to investigate the passage of oxidized low density lipoprotein (ox-LDL) through a tight monolayer of human umbilical vein endothelial cells (HUVEC) cultured on a collagen-coated filter. Passage of Dil-labeled ox-LDL through the monolayer was measured using a fluorescence spectrophotometer. The uptake and efflux of ox-LDL by HUVEC were determined using fluorescence microscopy and HPLC. Results: Caveolae inhibitors - carrageenan (250 μg/mL), filipin (5 μg/mL), and nocodazole (33 μmol/L)-decreased the transport of ox-LDL across the monolayer by 48.9%, 72.4%, and 79.8% as compared to the control group. In addition, they effectively decreased ox-LDL uptake and inhibited the efflux of ox-LDL. Caveolin-1 and LOX-1 were up-regulated by ox-LDL in a time-dependent manner and decreased gradually after depletion of ox-LDL (P<0.05). After treatment HUVEC with ox-LDL and silencing caveolin-1, NF-KB translocation to the nucleus was blocked and LOX-1 expression decreased (P<0.05). Conclusion: Caveolae can be a carrier for ox-LDL and may be involved in the uptake and transcytosis of ox-LDL by HUVEC
期刊：
Phytomedicine : international journal of phytotherapy and phytopharmacology,2010年17(3-4):233-240 ISSN：0944-7113
通讯作者：
Deng, CQ
作者机构：
[Deng, Chang-Qing] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Chen, Bei-Yang; Zhang, Guo-Min; Li, Hua] Hunan Univ Tradit Chinese Med, Dept Pathol, Changsha, Hunan, Peoples R China.;[Tang, Ying-Hong] Hunan Univ Tradit Chinese Med, Dept Pharmacol, Changsha, Hunan, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, CQ] Hunan Univ Tradit Chinese Med, Sch Integrated Chinese & Western Med, Pathophysiol Lab, Xiangzui Rd, Changsha, Hunan, Peoples R China.
关键词：
Total saponins of "panax notoginseng root";Atorvastatin;Vascular smooth muscle cell;Proliferating cell nuclear antigen;Cyclind D(1);Cycline;Extracellular matrix;Collagen I;Fibronectin;Matrix metalloproteinase-9;Tissue inhibitor metalloproteinase-1
摘要：
Aim of the study: the effect of total saponins of "panax notoginseng root" on aortic intimal hyperplasia and the expressions of cell cycle protein and extracellular matrix in rats Materials and methods: Sprague-Dawley rats were randomly divided into sham-operated, control, TSPN and atorvastatin group. Rat aorta intima in all groups were injured by insertion of domestic balloon catheter into the aortae except sham-operated rats. Drugs were administrated orally from the second day after vascular injury and continued for 14 days. The injured segments of aortae were collected on the sixteenth day after operation to observe the morphological changes of vascular structure and to examine the expressions of proliferating cell nuclear antigen(PCNA), cyclinD1, cyclinE, collagen I(Col-I), fibronect(FN), matrix metalloproteinase-9(MMP-9) and tissue inhibitor metalloproteinase-1(TIMP-1). Results: TPNS significantly inhibited the vascular intimal hyperplasia. TPNS significantly lowered the expression of PCNA, cyclinE, cyclinD1, FN and MMP-9. TPNS had no significant impacts on the expression of Col-I and TIMP-1. Conclusions: Our studies indicated that TSPN could inhibit vessel restenosis after vascular intimal injury, and its mechanisms may be related to the blockage of the excessive proliferation of VSMC, the reduction of ECM protein deposition in the endometrium, and the degradation of ECM protein. (C) 2009 Elsevier GmbH. All rights reserved.
期刊：
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION,2017年317(24):2502-2514 ISSN：0098-7484
通讯作者：
Wu, XK
作者机构：
[Liu, Jian-Ping] Centre for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China;[Ding, Cai-Fei] Centre for Reproductive Medicine, Zhejiang Province Hospital of Integrative Medicine, Hangzhou, China;[Fu, Ping] Department of Gynecology, Hangzhou City Hospital of Chinese Medicine, Hangzhou, China;[Sun, Yun] Department of Gynecology, Wenzhou City Hospital of Chinese Medicine, Wenzhou, China;[Hou, Li-Hui; Xie, Liang-Zhen; Kuang, Hong-Ying; Gao, Jing-Shu; Ma, Hong-Li] Department of Obstetrics and Gynecology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, China
通讯机构：
[Wu, XK] Heilongjiang Univ Chinese Med, Affiliated Hosp 1, Dept Obstet & Gynecol, Harbin 150040, Peoples R China.
摘要：
Importance: Acupuncture is used to induce ovulation in some women with polycystic ovary syndrome, without supporting clinical evidence. Objective: To assess whether active acupuncture, either alone or combined with clomiphene, increases the likelihood of live births among women with polycystic ovary syndrome. Design, Setting, and Participants: A double-blind (clomiphene vs placebo), single-blind (active vs control acupuncture) factorial trial was conducted at 21 sites (27 hospitals) in mainland China between July 6, 2012, and November 18, 2014, with 10 months of pregnancy follow-up until October 7, 2015. Chinese women with polycystic ovary syndrome were randomized in a 1:1:1:1 ratio to 4 groups. Interventions: Active or control acupuncture administered twice a week for 30 minutes per treatment and clomiphene or placebo administered for 5 days per cycle, for up to 4 cycles. The active acupuncture group received deep needle insertion with combined manual and low-frequency electrical stimulation; the control acupuncture group received superficial needle insertion, no manual stimulation, and mock electricity. Main Outcomes and Measures: The primary outcome was live birth. Secondary outcomes included adverse events. Results: Among the 1000 randomized women (mean [SD] age, 27.9 [3.3] years; mean [SD] body mass index, 24.2 [4.3]), 250 were randomized to each group; a total of 926 women (92.6%) completed the trial. Live births occurred in 69 of 235 women (29.4%) in the active acupuncture plus clomiphene group, 66 of 236 (28.0%) in the control acupuncture plus clomiphene group, 31 of 223 (13.9%) in the active acupuncture plus placebo group, and 39 of 232 (16.8%) in the control acupuncture plus placebo group. There was no significant interaction between active acupuncture and clomiphene (P = .39), so main effects were evaluated. The live birth rate was significantly higher in the women treated with clomiphene than with placebo (135 of 471 [28.7%] vs 70 of 455 [15.4%], respectively; difference, 13.3%; 95% CI, 8.0% to 18.5%) and not significantly different between women treated with active vs control acupuncture (100 of 458 [21.8%] vs 105 of 468 [22.4%], respectively; difference, -0.6%; 95% CI, -5.9% to 4.7%). Diarrhea and bruising were more common in patients receiving active acupuncture than control acupuncture (diarrhea: 25 of 500 [5.0%] vs 8 of 500 [1.6%], respectively; difference, 3.4%; 95% CI, 1.2% to 5.6%; bruising: 37 of 500 [7.4%] vs 9 of 500 [1.8%], respectively; difference, 5.6%; 95% CI, 3.0% to 8.2%). Conclusions and Relevance: Among Chinese women with polycystic ovary syndrome, the use of acupuncture with or without clomiphene, compared with control acupuncture and placebo, did not increase live births. This finding does not support acupuncture as an infertility treatment in such women. Trial Registration: clinicaltrials.gov Identifier: NCT01573858.
作者机构：
[Tao, Huai] Department of Biochemistry and Molecular Biology, Hunan University of Chinese Medicine, Changsha 410208, China;[Tang, Yamei; Tang, Aiguo; Li, Cunyan] Department of Laboratory, The Second Xiangya Hospital, Central South University, Changsha 410011, China;[Liu, Yong] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China. Electronic address: csuliuyong@163.com;[Chen, Zhiheng] Department of Pediatrics, The Third Xiangya Hospital, Central South University, Changsha 410013, China;[Zhang, Xianghui] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China
通讯机构：
[Liu, Yong] Mental Health Institute, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha 410011, China. Electronic address:
摘要：
Oxytocin (OT) was reported to affect cognitive and emotional behavior by action in ventral tegmental area (VTA) and other brain areas. However, it is still unclear how OT activates VTA and related midline nucleus. Here, using patch-clamp recording, we studied the effects of OT on neuron activity in VTA and interfascicular nucleus (IF). OT dose-dependently and selectively excited small neurons located in medial VTA and the majority of IF neurons but not large neurons in lateral VIA. We found the hyperpolarization-activated current (I-h) and the membrane capacitance of OT-sensitive neuron were significantly smaller than those of OT-insensitive neurons. The action potential width of 01-sensitive neurons was about half that of OT-insensitive neurons. The OT effect was blocked by the OT receptor antagonist atosiban and WAY-267464 but not by tetrodotoxin, suggesting a direct postsynaptic activation of OT receptors. In addition, the phospholipase C (PLC) inhibitor U73122 antagonized the depolarization by OT. Both the nonselective cation channel (NSCC) antagonist SKF96365 and the Na+-Ca2+ exchanger (NCX) blocker SN-6 attenuated OT effects. These results suggested that the PLC signaling pathway coupling to NSCC and NCX contributes to the OT-mediated activation of neurons in medial VIA and IF. Taken together, our results indicate OT directly acted on medial WA and especially IF neurons to activate NSCC and NCX via PLC. The direct activation by OT of midbrain neurons may be one mechanism underlying OT effects on social behavior. (C) 2013 Elsevier Ltd. All rights reserved.
期刊：
EXPERIMENTAL AND THERAPEUTIC MEDICINE,2014年8(1):3-8 ISSN：1792-0981
通讯作者：
Qin, L
作者机构：
[Zhu, Neng; Guo, Qiong; Yang, Luoyan] Cent S Univ, Xiangya Hosp 2, Dept Urol, Changsha 410011, Hunan, Peoples R China.;[Zhu, Neng; Luo, Zhigang] South China Univ, Affiliated Hosp 2, Dept Urol, Hengyang 421001, Hunan, Peoples R China.;[Qin, Li] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.;[Qin, Li; Liao, Duanfang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Qin, L] Univ South China, Inst Pharm & Pharmacol, 28 Changsheng West Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
Wnt5a;cancer;metastasis;signaling pathway
摘要：
Wnt5a is a noncanonical signaling member of the wingless-related/mouse mammary tumor virus integration family, which is involved in a wide range of cellular processes, particularly in cancer development and metastasis. Accumulating evidence indicates that Wnt5a exhibits paradoxical effects in various types of cancer metastasis. Therefore, the Wnt5a signaling cascade in cancer metastasis appears to be complex and may depend on binding receptors, downstream effectors, exogenous inhibitors and tumor micro-environments, as well as the extracellular matrix, particularly cell/tissue-tropic contexts. The aim of the present study was to summarize the previous findings on the roles of Wnt5a and the potential mechanisms in various types of cancer metastasis. Furthermore, it is reasonable to hypothesize that Wnt5a and the involved signaling pathways may become molecular targets in the treatment of cancer metastasis.
期刊：
SOCIAL BEHAVIOR AND PERSONALITY,2013年41(7):1141-1152 ISSN：0301-2212
通讯作者：
Cai, TS
作者机构：
[Zhang, Bin] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Cai, Taisheng; Zhu, Hong; Zhou, Xueting] Cent S Univ, Inst Med Psychol, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Zhou, Xueting] Changsha Univ Sci & Technol, Sch Foreign Languages, Changsha, Hunan, Peoples R China.
通讯机构：
[Cai, TS] Cent S Univ, Inst Med Psychol, Xiangya Hosp 2, Middle Ren Min Rd 139, Changsha 410011, Hunan, Peoples R China.
关键词：
perfectionism;perceived social support;depression;anxiety;college students
摘要：
We examined the role of perceived social support in the relationship between perfectionism and depression/anxiety. Partial correlation and hierarchical regression were conducted using cross-sectional data from 426 college students. They completed questionnaires including positive and negative perfectionism scales, the Depression Anxiety Stress Scale-21, and the Multidimensional Scale of Perceived Social Support. Results showed that depression/anxiety were significantly correlated with perceived social support and perfectionism. Perceived social support significantly moderated the influence of perfectionism upon depression/anxiety. These findings indicate that perceived social support may have a protective effect in preventing perfectionists from experiencing depression and anxiety.
摘要：
It has been reported that the effect of inflammatory cytokines on beta-cell destruction in type 1 diabetes is concentration-dependent. However, the underlying mechanisms remain unclear. In the present study, we found that a high concentration of cytokines promoted apoptosis in the rat beta-cell line INS-1, whereas a low concentration of cytokines had no effect. We also found that cytokines at a low concentration stimulated neutral ceramidase (NCDase) release via exosomes from INS-1 cells, whereas cytokines at a high concentration inhibited NCDase release. Furthermore, the results showed that the NCDase-containing exosomes isolated from the culture medium of INS-1 cells treated with cytokines at a low concentration inhibited apoptosis induced by a high concentration of cytokines. Finally, the results also showed that the protective action of NCDase in the exosomes on apoptosis was mediated by the generation of sphingosine 1-phosphate (S1P) and its interaction with S1P receptor 2. Taken together, these findings revealed a novel NCDase-S1P-phosphate-S1P receptor 2-dependent mechanism by which a low level of inflammatory cytokines protects pancreatic beta-cells from apoptosis induced by a high level of inflammatory cytokines.
通讯机构：
[Luo, XR] Cent S Univ, Mental Hlth Inst, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.
摘要：
Eye-tracking studies in young children with autism spectrum disorder (ASD) have shown a visual attention preference for geometric patterns when viewing paired dynamic social images (DSIs) and dynamic geometric images (DGIs). In the present study, eye-tracking of two different paired presentations of DSIs and DGIs was monitored in a group of 13 children aged 4 to 6 years with ASD and 20 chronologically age-matched typically developing children (TDC). The results indicated that compared with the control group, children with ASD attended significantly less to DSIs showing two or more children playing than to similar DSIs showing a single child. Visual attention preference in 4- to 6-year-old children with ASDs, therefore, appears to be modulated by the type of visual stimuli.
摘要：
Cholesterol accumulation is a critical step during the development and progression of atherosclerosis. Recently, Wnt5a expression has been found to be markedly upregulated in both murine and human atherosclerotic lesions. However, the effect and mechanism of Wnt5a in atherosclerosis is poorly understood. In the present study, we investigated the effects and potential mechanisms of Wnt5a on cholesterol accumulation during atherosclerosis. We used RAW264.7 and vascular smooth muscle cells (VSMC) treated with oxidized low-density lipoprotein (oxLDL) as lipid-loaded cell models. We found that expression of Wnt5a protein was increased in a concentration (25, 50, 75 and 100 mug/mL)- and time (24, 48 and 72 h)-dependent manner by oxLDL treatment. To explore the underlying mechanism, we used Wnt5a short interference (si) RNA to knockdown Wnt5a expression in both RAW264.7 cells and VSMC, or applied recombinant Wnt5a (rWnt5a) to stimulate Wnt5a signalling. After Wnt5a knockdown, total cholesterol (TC) and free cholesterol (FC) content in both cell types increased significantly (P < 0.05) upon exposure to oxLDL. Conversely, the TC and FC content decreased markedly (P < 0.05) after treatment of cells with rWnt5a. More importantly, both protein and mRNA expression of Caveolin-1 and ATP-binding cassette transporter A1 (ABCA1) was significantly reduced after exposure of wnt5a siRNA-treated cells to oxLDL, whereas rWnt5a treatment of cells resulted in increased Caveolin-1 and ABCA1 protein expression after exposure of cells to oxLDL. Together, these findings demonstrate, for the first time, that Wnt5a reduces the accumulation of cholesterol in lipid-loaded cells by regulating the mRNA expression of Caveolin-1 and ABCA1, which are involved in reverse cholesterol transport. This may present a novel mechanism of Wnt5a-mediated cholesterol transportation in macrophages and VSMC. Therefore, targeting the Wnt5a signalling pathway may have clinical implications in atherosclerosis.
摘要：
Aim: To evaluate the effects of angiopoietin-1 (Ang-1) on myocardial endothelial cell function under high glucose (HG) condition. Methods: Mouse heart myocardial endothelial cells (MHMECs) were cultured and incubated under HG (25 mmol/L) or normal glucose (NG, 5 mmol/L) conditions for 72 h. MTT was used to determine cellular viability, and TUNEL assay and caspase-3 enzyme linked immunosorbent assays were used to assay endothelial apoptosis induced by serum starvation. Immunoprecipitation and Western blot analysis were used to analyze protein phosphorylation and expression. Endothelial tube formation was used as an in vitro assay for angiogenesis. Results: Exposure of MHMECs to HG resulted in dramatic decreases in phosphorylation of the Tie-2 receptor and its downstream signaling partners, Akt/eNOS, compared to that under NG conditions. Ang-1 (250 ng/mL) increased Tie-2 activation, inhibited cell apoptosis, and promoted angiogenesis. Ang-1-mediated protection of endothelial function was blunted by Ang-2 (25 ng/mL). Conclusion: Ang-1 activates the Tie-2 pathway and restores hyperglycemia-induced myocardial microvascular endothelial dysfunction. This suggests a protective role of Ang-1 in the ischemic myocardium, particularly in hearts affected by hyperglycemia or diabetes
期刊：
Clinica chimica acta; international journal of clinical chemistry,2016年459:137-146 ISSN：0009-8981
通讯作者：
Qin, Li
作者机构：
[Qin, Li] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China. Electronic address: lqin1011@126.com;[Liu, Zheng; Liao, Duan-Fang; Ao, Bao-Xue; Shi, Ya-Ning; Zheng, Xi-Long; Liu, Chan] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China;[Liu, Zheng; Ao, Bao-Xue; Liu, Chan] Institute of Pharmacy and Pharmacology, University of South China, Hengyang, Hunan, China;[Wu, Hong-Tao] The Second Xiangya Hospital, Central South University, Changsha, China;[Zheng, Xi-Long] Department of Biochemistry & Molecular Biology, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
通讯机构：
[Qin, Li] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, China. Electronic address:
摘要：
Steroid receptor RNA activator (SRA) is a type of long noncoding RNA (lncRNA) which coordinates the functions of various transcription factors, enhances steroid receptor-dependent gene expression, and also serves as a distinct scaffold. The novel, profound and expanded roles of SRA are emerging in critical aspects of coactivation of nuclear receptors (NRs). As a nuclear receptor coactivator, SRA can coactivate androgen receptor (AR), estrogen receptor alpha (ER alpha), ER beta, progesterone receptor (PR), glucocorticoid receptor (GR), thyroid hormone receptor and retinoic acid receptor (RAR). Although SRA is one of the least well-understood molecules, increasing studies have revealed that SRA plays a key role in both biological processes, such as myogenesis and steroidogenesis, and pathological changes, including obesity, cardiomyopathy, and tumorigenesis. Furthermore, the SRA-related signaling pathways, such as the mitogen-activated protein kinase (p38 MAPK), Notch and tumor necrosis factor alpha (TNF alpha) pathways, play critical roles in the pathogenesis of estrogen-dependent breast cancers. In addition, the most recent data demonstrates that SRA expression may serve as a new prognostic marker in patients with ER-positive breast cancer. Thus, elucidating the molecular mechanisms underlying SRA-mediated functions is important to develop proper novel strategies to target SRA in the diagnosis and treatment of human diseases. (C) 2016 Elsevier B.V. All rights reserved.
期刊：
Journal of ethnopharmacology,2011年135(1):7-14 ISSN：0378-8741
通讯作者：
Deng, CQ
作者机构：
[Deng, Chang-Qing; Chen, Gang] Hunan Univ Tradit Chinese Med, Pathophysiol Lab, Changsha, Hunan, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Changsha, Hunan, Peoples R China.
通讯机构：
[Deng, CQ] Hunan Univ Tradit Chinese Med, Pathophysiol Lab, Shaoshan Rd 113, Changsha, Hunan, Peoples R China.
关键词：
BuYang HuanWu Decoction;Vascular smooth muscle cell;Platelet derived growth factor;Proliferating cell nuclear antigen;c-fos;CyclinD(1);Extracellular regulated protein kinases1/2;Mitogen-activated protein kinase;phosphatase-1
摘要：
Buyang Huanwu Decoction (BYHWD) was a commonly used traditional Chinese medicine for the treatment and prevention of ischemic cardiovascular and cerebral disease. Previous studies had shown that BYHWD alkaloids and glycosides could inhibit intimal hyperplasia and vascular smooth muscle cell (VSMC) proliferation after injury caused by balloon catheter. The present study aims to explore the mechanisms by which cell cycle was affected by BYHWD and its components. Primary rat VSMC was treated with platelet-derived growth factor (PDGF) and cell cycle phase and extracellular-signal regulated protein kinase (ERK) transduction pathway factors were measured. PDGF-treated cells were associated with a significant increase in the number of cells in the G(2)/M phase and S phase, and in the expression of P-ERK1/2, proliferating cell nuclear antigen (PCNA), c-fos, cyclinD(1) and cyclin-dependent kinase-4, as well as a decrease in the number of cells in the G(0)/C(1) phase, and in the expression of cyclin-dependent kinase inhibitor P21 protein and mitogen-activated protein kinase phosphatase-1 (MKP-1). Treatment with plasma of rats fed seven doses of BYHWD crude extract (22.2 g/kg), BYHWD alkaloids (1.66 g/kg), BYHWD glycosides (14.2 g/kg) or the negative control atorvastatin (20 mg/kg) inhibited these changes. All drug-containing plasma had similar activity to the mitogen activated protein kinase (MAPK)/ERK antagonist PD098059 which inhibited PDGF-induced expression of P-ERK1/2 and enhanced MKP-1. These suggest that BYHWD and its components may prevent VSMC proliferation by interfering with the ERK transduction pathway. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
期刊：
BIOMED RESEARCH INTERNATIONAL,2014年2014:531979 ISSN：2314-6133
通讯作者：
Fan, JM
作者机构：
[Zhang, Wen; Fan, Jianmin] The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[Liu, Qiming] Cardiovascular Medicine Department, The Second Xiangya Hospital of Central South University, Changsha 410011, China
通讯机构：
[Fan, JM] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
摘要：
Human cytomegalovirus (HCMV) infection is linked to the development and severity of the cardiovascular disease atherosclerosis; however, there is little known about the promotion of atherosclerosis. miR-US25-1 is one of HCMV-encoded miRNAs and targets cellular genes that are essential for virus growth to control the life cycle of the virus and host cells. The prominent regulation on cell cycle genes of the miR-US25-1 attracts us to explore its role in the atherosclerosis promotion. It was indicated that miR-US25-1 level was upregulated in subjects or in endothelial cells with HCMV infection; and the miR-US25-1 downregulated the expression of BRCC 3 by targeting the 5' UTR of BRCC 3. And a miR-US25-1 mimics transfection could reduce the EAhy926 cell viability but did not induce apoptosis in EAhy926 cells. And what is more, miR-US25-1 mimicis transfection deteriorated the ox-LDL-induced apoptosis and aggravated the upregulation of apoptosis-associated molecules by oxidised low density lipoprotein (ox-LDL) in EAhy926 cells. And we have also confirmed the deregulation of BRCC 3 expression by miR-US25-1 by targeting the 5' UTR of it. Given the vital role of BRCC 3 in DNA damage repairing, we speculated that the targeting inhibition of BRCC 3 by miR-US25-1 may contribute to the aggravation of ox-LDL-promoted apoptosis of endothelial EAhy926 cells.
作者机构：
[Wu, Lixiang; Tang, Qingping; Li, Simin] Cent S Univ, Sch Basic Med Sci, Dept Physiol, Changsha 410008, Hunan, Peoples R China.;[Yang, Xiaosu; Nie, Jingjing; Shen, Qin] Cent S Univ, Xiang Ya Hosp, Dept Neurol, Changsha 410008, Hunan, Peoples R China.;[Huang, Xiaosong; Tan, Lihong] Hunan Univ Chinese Med, Brain Hosp Hunan Prov, Dept Neurol, Changsha 410007, Hunan, Peoples R China.;[Tang, Qingping] Hunan Univ Chinese Med, Brain Hosp Hunan Prov, Dept Rehabil, Changsha 410007, Hunan, Peoples R China.;[Chen, Hengheng] Chongqing Med Univ, Childrens Hosp, Key Lab Child Dev & Disorders, Minist Educ, Chongqing 400014, Peoples R China.
通讯机构：
[Wu, LX] Cent S Univ, Sch Basic Med Sci, Dept Physiol, Xiang Ya Rd 90, Changsha 410008, Hunan, Peoples R China.
关键词：
Motor learning;Long-term depression;Stroke;Synaptic plasticity;PICK1;Willed-movement training
摘要：
Willed-movement (WM) training has been implicated in the promotion of motor function in human stroke survivors and focal ischemic rats. However, the molecular basis of changes in synaptic transmission following WM training remains unclear. In addition, studies examining the influence of rehabilitative training, such as skilled motor learning, on long-term depression (LTD) of synapses in the primary motor cortex have produced conflicting results. To identify the possible effects of willed movement on motor recovery, on expression of the protein interacting with C kinase 1 protein (PICK1), and on PICK1 related LTD, littermate rats were randomly divided into four groups: normal control, middle cerebral artery occlusion (MCAO), WM and environmental modification. Neurological and neurobehavioral assessments were performed for the rats with occlusion of the right middle cerebral artery. Double-labeling immunofluorescence staining was performed to detected expression of PICK1 and NeuN. Extracellular recordings were used to detect the basal extracellular field excitatory postsynaptic potentials and LTD with or without PICK1 inhibitor FSC231. The results showed that willed-movement training facilitated motor recovery after MCAO in rats, increased the PICK1 protein levels, and enhanced LTD in the ischemia hemisphere. The enhanced LTD for the rats after willed-movement training was attenuated by FSC231. Our results indicated that willed-movement training can enhance activity-dependent LTD through PICK1-dependent mechanisms in the ischemic hemisphere of rats. (C) 2013 Elsevier B.V. All rights reserved.
摘要：
Proliferation of vascular smooth muscle cells (VSMCs) contributes to the development of atherosclerosis. Ezetimibe is a new lipid lowering agent that inhibits cholesterol absorption. In the present study we attempted to investigate whether ezetimibe has any effect on VSMC proliferation and the potential mechanisms involved. Our data showed ezetimibe abrogated the proliferation and migration of primary rat VSMCs induced by Chol:M beta CD. Mechanically, we found that ezetimibe was capable of abolishing cyclin D1, CDK2, phospho-Rb (p-Rb), and E2F protein expressions that were upregulated by Chol:M beta CD treatment. In addition, Ezetimibe was able to reverse cell cycle progression induced by Chol:M beta CD, which was further supported by its down-regulation of cyclin D1 promoter activity in the presence of Chol:M beta CD. Furthermore, ezetimibe abrogated the increment of phospho-ERK1/2 (p-ERK1/2) and nuclear accumulation of ERK1/2 in VSMCs induced by Chol:M beta CD Inhibition of the MAPK pathway by using ERK1/2 inhibitor PD98059 attenuated the reduction effect of ezetimibe on the expressions of phosphor-MEK1 (p-MEK1), p-ERK1/2, and cyclin Dl. Taken together our data suggest that ezetimibe inhibits Chol:M beta CD-induced VSMCs proliferation and leads to cell cycle arrest at the G0/G1 phase by suppressing cyclin D1 expression via the MAPK signaling pathway. These novel findings support the potential pleiotropic effect of ezetimibe in cardiovascular disease.
摘要：
T helper 2 (Th2) polarization is a major pathological feature in allergic diseases; its etiology is not fully understood. This study aims to elucidate the adjuvant effect of the microbial product-derived small peptides in the initiation of antigen-specific Th2 polarization. In this study, a clinical survey of patients with chronic rhinosinusitis (CRS) and food allergy (FA) was carried out. The Staphylococcal enterotoxin B (SEB)-derived small peptides (Ssps) were examined in the human stool extracts. The formation of Ssp/antigen adducts was tested in a protein-protein combination assay. The bone marrow-derived dendritic cells (BMDCs) were employed to test the role of Ssp/ovalbumin (OVA) adducts in the dendritic cell (DC) maturation. A mouse model was developed to test the role of Ssp/OVA adducts in the initiation of Th2 polarization in the intestine. The results showed that 54 (18.2%) patients with FA were diagnosed among 296 patients with SEB+ CRS; only eight (2.9%) FA patients were identified among 272 patients with SEB 2 CRS. Ssps were detected in the stool protein extracts from FA patients with SEB+ CRS, but not in those with SEB- CRS. Ssp/OVA adducts induced DC maturation, speeded up DC migration, activated CD4(+) T cells in the regional lymph nodes and induced skewed Th2 polarization in the local tissue. We conclude that patients with SEB+ CRS are prone to suffering from FA. SEB- can be degraded to Ssps in the gastrointestinal tract. The Ssps can bind macromolecular antigens to form adducts to promote the antigenicity of the antigens and induction of the antigen-specific Th2 polarization and inflammation in the local tissue. Cellular & Molecular Immunology (2013) 10, 78-83; doi:10.1038/cmi.2012.32; published online 3 September 2012
作者：
Zhu, Qun;Shen, Bo;Zhang, Boshao;Zhang, Wei;Chin, Steve H.;Jin, Junfei;Liao, Duan-fang
期刊：
Molecular and Cellular Biochemistry,2010年340(1-2):275-281 ISSN：0300-8177
通讯作者：
Jin, JF
作者机构：
[Zhu, Qun] Nanjing Med Univ, Affiliated Hosp 2, Dept Endocrinol, Nanjing 210011, Jiangsu, Peoples R China.;[Shen, Bo] Jiangsu Canc Hosp, Dept Med Oncol, Nanjing 210009, Jiangsu, Peoples R China.;[Zhang, Boshao] Univ Alabama, Dept Biostat, Birmingham, AL 35294 USA.;[Zhang, Wei] Southeast Univ, Sch Med, Dept Pathophysiol, Nanjing 210009, Jiangsu, Peoples R China.;[Liao, Duan-fang; Jin, Junfei] Univ S China, Res Ctr Life Sci, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Jin, JF] Univ S China, Res Ctr Life Sci, 28 W Changsheng Rd, Hengyang 421001, Hunan, Peoples R China.
关键词：
AMP-activated protein kinase;Doxorubicin;Human leukemia K562 cell;Apoptosis;Cell death
摘要：
Doxorubicin (Dox) is a commonly used anthracycline in many antitumor regimens. The dose related Dox-induced cardiotoxicity often poses challenge in clinical practice, lowering its dose and administering it in combination with other compound is an option. In this study, we found that a nontoxic concentration of Dox at 34.5 nM (20 ng/ml) combined with Compound C, an inhibitor used in AMP-activated protein kinase (AMPK) pathway, could kill human leukemia K562 cells. Additionally, this study confirmed that the combined effect was related to the inhibition of some key proteins such as AMPK and acetyl CoA carboxylase. Moreover, down-regulation of these key proteins in AMPK pathway using siRNA technology also sensitized K562 cells to nontoxic concentration of Dox. The study also showed that Dox at a concentration of 345.0 nM (200 ng/ml) or 862.0 nM (500 ng/ml) that is lower than a typical value of 1-2 microM Dox in patients could kill human leukemia K562 cells. Taken together, our results suggest that inhibition of AMPK pathway by Compound C or siRNA sensitizes K562 cells to nontoxic concentration of Dox which is much lower than typical concentration in plasma of clinical patients.
作者机构：
[Shi, Lijuan; Gong, Jingbo; Luo, Xuerong; Cui, Xilong; Wang, Suhong] Mental Health Institute of The Second Xiangya Hospital, National Technology Institute of Psychiatry, Key Laboratory of Psychiatry and Mental Health of Hunan Province, Central South University, Changsha, Hunan, China;[Gong, Jingbo] Traditional Chinese Medicine University of Hunan, Changsha, Hunan, PR China;[Wang, Suhong] Department of Neuroscience, the Third Affiliated Hospital of Soochow University, Changzhou, China;[Yuan, Jiajin] School of Psychology, Southwest University, Chongqing, China
摘要：
OBJECTIVE: The current model of ADHD suggests abnormal reward and punishment sensitivity, although differences in ADHD subgroups are unclear. This study aimed to investigate the effect of feedback valence (reward or punishment) and punishment magnitude (small or large) on Feedback-Related Negativity (FRN) and Late Positive Potential (LPP) in two subtypes of ADHD (ADHD-C and ADHD-I) compared to typically developing children (TD) during a children's gambling task. METHODS: Children with ADHD-C (n = 16), children with ADHD-I (n = 15) and typically developing children (n = 15) performed a children's gambling task under three feedback conditions: large losses, small losses and gains. FRN and LPP components in brain potentials were recorded and analyzed. RESULTS: In TD children and children with ADHD-C, large loss feedback evoked more negative FRN amplitudes than small loss feedback, suggesting that brain sensitivity to the punishment and its magnitude is not impaired in children with ADHD-C. In contrast to these two groups, the FRN effect was absent in children with ADHD-I. The LPP amplitudes were larger in children with ADHD-C in comparison with those with ADHD-I, regardless of feedback valence and magnitude. CONCLUSION: Children with ADHD-C exhibit intact brain sensitivity to punishment similar to TD children. In contrast, children with ADHD-I are significantly impaired in neural sensitivity to the feedback stimuli and in particular, to punishment, compared to TD and ADHD-C children. Thus, FRN, rather than LPP, is a reliable index of the difference in reward and punishment sensitivity across different ADHD-subcategories.
