作者机构：
[Huang, Yuxiang; Tang, Jianghong; Huang, YX] Hunan Univ Chinese Med, Dept Geriatr, Affiliated Hosp 2, Changsha 410005, Hunan, Peoples R China.;[Zhu, Shilin] Hunan Univ Chinese Med, Dept Nursing, Affiliated Hosp 2, Changsha 410005, Hunan, Peoples R China.
通讯机构：
[Huang, YX ] H;Hunan Univ Chinese Med, Dept Geriatr, Affiliated Hosp 2, Changsha 410005, Hunan, Peoples R China.
关键词：
geriatric depression;quality of life;symptom burden;transitional care
摘要：
OBJECTIVE: To explore the effects of smartphone-based hospital-family transitional care on symptom burden and quality of life in elderly patients with depression. METHODS: This study retrospective analyzed the clinical data of 168 elderly patients with depression admitted to our hospital from January 2022 to January 2024. A total of 79 patients were included in the reference group (routine transitional management), and 89 subjects were included in the observation group (smartphone-based hospital-family transitional care). The symptom burden and quality of life in both groups before and after management were compared. The main statistical methods used in this study were the chi-squared test and the Mann-Whitney U test. RESULTS: Before discharge, no significant difference existed in Geriatric Depression Scale (GDS) scores, P300 latency, P300 amplitude, Montreal Cognitive Assessment (MoCA) scores, and the scores of each domain in the World Health Organization Quality of Life (WHOQOL)-BREF between the two groups (all p > 0.05). After 5 months, the observation group demonstrated a significantly lower GDS score (p = 0.016), shorter P300 latency (p < 0.001), higher P300 amplitude (p < 0.001), higher MoCA score (p = 0.001), and significantly higher scores in physiological, psychological, and environmental domains than the reference group (p < 0.001), with no significant difference in social relation domain (p > 0.05). CONCLUSIONS: Smartphone-based hospital-family transitional care can improve the symptom burden, cognitive function, and quality of life of elderly patients with depression.
作者机构：
[Li, Ze; Song, Zhenyan; Cheng, Shaowu; He, Jiawei; Li, Ping; Wu, Yixiao; Cheng, SW; Chen, Qi; Wang, Shiwei; Yu, Wenjing] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Peoples R China.;[Li, Ze; Song, Zhenyan; He, Jiawei; Li, Ping; Wu, Yixiao; Chen, Qi; Wang, Shiwei; Yu, Wenjing] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha 410208, Peoples R China.;[Cheng, Shaowu; Cheng, SW] Hunan Univ Chinese Med, Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Changsha 410208, Peoples R China.;[Cheng, Shaowu; Cheng, SW] Hunan Univ Chinese Med, Sch Med, Changsha 410208, Peoples R China.
通讯机构：
[Cheng, SW ] H;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Hunan Univ Tradit Chinese Med, Affiliated Hosp 2, Changsha 410208, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Changsha 410208, Peoples R China.
摘要：
Emerging contaminants refer to chemical substances that have not been widely regulated but possess the potential to cause adverse effects on both the environment and human health. Antibiotics, as emerging contaminants, pose significant threats to ecosystems and human health due to their widespread use and persistence in the environment. Levofloxacin, a broad-spectrum fluoroquinolone antibiotic, is commonly employed in the treatment of bacterial infections, and has been frequently detected in environmental matrices and freshwater systems. In this study, we assessed the effects of levofloxacin on hatchability, mortality rates, malformations, behavioral changes, and cardiac development in zebrafish embryos by exposing them to varying concentrations of levofloxacin (0, 0.5, 1, 2, 4, and 8 mM). Our results demonstrate that levofloxacin exposure significantly impaired the growth and development of zebrafish larvae, particularly at higher concentrations. Notable effects included reduced body length, abnormal yolk sac and swim bladder development, pericardial edema, prolonged distances between the sinus venosus and arteriolar bulb (SV-BA), and disruptions in heart rate. Quantitative PCR analysis further revealed that levofloxacin exposure significantly upregulated the expression of key cardiac development genes in zebrafish larvae, including nppa, myh6, cacna1ab, myl7, gata4, nkx2.5, tbx2b, and tbx5b. These findings indicate that levofloxacin exposure exerts significant toxic effects on both embryonic and larval growth as well as heart development and gene expression in zebrafish. This study provides critical insights into the potential ecological risks posed by levofloxacin along with other antibiotics while laying a foundation for further investigation into their toxicological mechanisms.
摘要：
This study was designed to investigate the role of Ligustrum lucidum Ait and Ecliptae Herba on premature ovarian failure (POF) and the underlying mechanisms. In the POF mouse model constructed using cyclophosphamide (CTX), Ligustrum lucidum Ait and Ecliptae Herba increased ovarian index and estradiol (E2) levels and curtailed motility and follicle-stimulating hormone (FSH). Ligustrum lucidum Ait and Ecliptae Herba alleviated ovarian pathological damage in POF mice and promoted the expression of ovarian CD31 and Vascular Endothelial Growth Factor A (VEGFA). Through high-performance liquid chromatography-mass spectrometry (HPLC-MS) and network pharmacology, Specnuezhenide and ecliptasaponin A were identified as the key components of Ligustrum lucidum Ait and Ecliptae Herba in anti-POF action. The important target associated with these components is Estrogen Receptor (ESR) 1. Molecular docking and in vitro experiments showed that Specnuezhenide and ecliptasaponin A can both bind to the ESR protein; knocking down ESR1 inhibited the anti-apoptotic effect of Specnuezhenide and ecliptasaponin A on CTX-induced POF cells. In conclusion, the key components of Ligustrum lucidum Ait and Ecliptae Herba that alleviate POF are Specnuezhenide and ecliptasaponin A, which improve the condition by upregulating ESR1.
This study was designed to investigate the role of Ligustrum lucidum Ait and Ecliptae Herba on premature ovarian failure (POF) and the underlying mechanisms. In the POF mouse model constructed using cyclophosphamide (CTX), Ligustrum lucidum Ait and Ecliptae Herba increased ovarian index and estradiol (E2) levels and curtailed motility and follicle-stimulating hormone (FSH). Ligustrum lucidum Ait and Ecliptae Herba alleviated ovarian pathological damage in POF mice and promoted the expression of ovarian CD31 and Vascular Endothelial Growth Factor A (VEGFA). Through high-performance liquid chromatography-mass spectrometry (HPLC-MS) and network pharmacology, Specnuezhenide and ecliptasaponin A were identified as the key components of Ligustrum lucidum Ait and Ecliptae Herba in anti-POF action. The important target associated with these components is Estrogen Receptor (ESR) 1. Molecular docking and in vitro experiments showed that Specnuezhenide and ecliptasaponin A can both bind to the ESR protein; knocking down ESR1 inhibited the anti-apoptotic effect of Specnuezhenide and ecliptasaponin A on CTX-induced POF cells. In conclusion, the key components of Ligustrum lucidum Ait and Ecliptae Herba that alleviate POF are Specnuezhenide and ecliptasaponin A, which improve the condition by upregulating ESR1.
摘要：
Post-stroke depression (PSD) is one of the most common and devastating neuropsychiatric complications in stroke patients, affecting more than one-third of survivors of ischemic stroke (IS). Despite its high incidence, PSD is often overlooked or undertreated in clinical practice, and effective preventive measures and therapeutic interventions remain limited. Although the exact mechanisms of PSD are not fully understood, emerging evidence suggests that the gut microbiota plays a key role in regulating gut-brain communication. This has sparked great interest in the relationship between the microbiota-gut-brain axis (MGBA) and PSD, especially in the context of cerebral ischemia. In addition to the gut microbiota, another important factor is the gut barrier, which acts as a frontline sensor distinguishing between beneficial and harmful microbes, regulating inflammatory responses and immunomodulation. Based on this, this paper proposes a new approach, the microbiota-immune-barrier axis, which is not only closely related to the pathophysiology of IS but may also play a critical role in the occurrence and progression of PSD. This review aims to systematically analyze how the gut microbiota affects the integrity and function of the barrier after IS through inflammatory responses and immunomodulation, leading to the production or exacerbation of depressive symptoms in the context of cerebral ischemia. In addition, we will explore existing technologies that can assess the MGBA and potential therapeutic strategies for PSD, with the hope of providing new insights for future research and clinical interventions.
通讯机构：
[Chen, Z; Deng, GM ] H;Hunan Univ Chinese Med, Hosp 1, Changsha 410007, Hunan, Peoples R China.;Hunan Univ Chinese Med, Hosp 2, Changsha 410005, Hunan, Peoples R China.
关键词：
traditional Chinese medicine;tumor;nanodelivery system;tumor therapy
摘要：
For centuries, traditional Chinese medicine (TCM) has had certain advantages in the treatment of tumors. However, due to their poor water solubility, low bioavailability and potential toxicity, their effective delivery to target sites can be a major challenge. Nanocarriers based on the active ingredients of TCM, such as liposomes, polymer nanoparticles, inorganic nanoparticles, and organic/inorganic nanohybrids, are a promising strategy to improve the delivery of TCM, resulting in higher therapeutic outcomes and fewer side effects. Therefore, this article intends to review the application of Chinese medicine nano preparation in tumor. Firstly, we introduce the classification and synthesis of nanometer preparations of Chinese medicine. The second part mainly introduces the different responses of TCM nano-preparations in the course of treatment to introduce how TCM nano-preparations play a role in anti-tumor therapy. The third part focuses on Different response modes of Chinese medicine nano preparations in tumor therapy. The fourth part elucidates the application of Chinese medicine nano preparations in the treatment of cancer. Finally, the research direction to be explored in related fields is put forward.
摘要：
BACKGROUND: Upper eyelid ptosis is a common aesthetic concern among Asian patients, resulting in a tired and drowsy appearance that affects their attractiveness. The levator advancement technique is widely used for ptosis correction; however, achieving precise results remains challenging. OBJECTIVES: This study introduces a modified approach to improve the accuracy of levator aponeurosis advancement by focusing on precise measurements and anterior displacement within a defined corneal range. METHODS: The study included patients with mild to moderate ptosis. We measured the mean margin reflex distance 1 (MRD-1) preoperatively and 12months postoperatively. Histological evidence was obtained through staining of levator aponeurosis complexes using Hematoxylin and Eosin and Masson's trichrome. RESULTS: In this prospective study, 29 patients showed a significant increase in mean MRD-1 from 2.56 ± 0.84mm (range 1.02-3.98mm) preoperatively to 4.38 ± 0.55mm (range 3.09-5.35mm) at the 12-month follow-up (paired t-test; P < 0.001). Masson's staining revealed that the levator aponeurosis tissue primarily consists of collagen fibers, which minimize potential errors due to their toughness and lack of elasticity during the surgical procedure. CONCLUSIONS: The modified technique enhances the accuracy of levator aponeurosis advancement in ptosis correction, resulting in minimal surgical trauma and high patient satisfaction. Our technique appears to have a low incidence of severe adverse effects, based on the available data from the current case series. LEVEL OF EVIDENCE IV: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
摘要：
Purpose The traditional N staging system fails to adequately stratify the prognostic heterogeneity in patients with resectable node-positive, stage III, non-small cell lung cancer, particularly in those undergoing postoperative radiotherapy. The purpose of this study was to determine the optimal nodal status classification strategy: the traditional N classification, the positive lymph nodes-based classification, or the lymph node ratio-based classification. Furthermore, we aimed to identify the population that would benefit the most from postoperative radiotherapy using the best classification strategy.
The traditional N staging system fails to adequately stratify the prognostic heterogeneity in patients with resectable node-positive, stage III, non-small cell lung cancer, particularly in those undergoing postoperative radiotherapy. The purpose of this study was to determine the optimal nodal status classification strategy: the traditional N classification, the positive lymph nodes-based classification, or the lymph node ratio-based classification. Furthermore, we aimed to identify the population that would benefit the most from postoperative radiotherapy using the best classification strategy.
Methods We analysed data from 5028 patients with resectable node-positive, stage III, non-small cell lung cancer sourced from the Surveillance, Epidemiology, and End Results (SEER) database. Various lymph node staging systems, including traditional N staging, classification based on the number of positive lymph nodes, and classification based on the lymph node ratio, were incorporated into the prognostic prediction model. Survival outcomes were evaluated using lung cancer-specific survival and Kaplan-Meier analysis.
We analysed data from 5028 patients with resectable node-positive, stage III, non-small cell lung cancer sourced from the Surveillance, Epidemiology, and End Results (SEER) database. Various lymph node staging systems, including traditional N staging, classification based on the number of positive lymph nodes, and classification based on the lymph node ratio, were incorporated into the prognostic prediction model. Survival outcomes were evaluated using lung cancer-specific survival and Kaplan-Meier analysis.
Results The lymph node ratio classification model demonstrated the highest prognostic prediction performance, with the highest C-index, area under the curve, and the lowest Akaike information criterion, followed by the positive lymph nodes classification model and the traditional N staging model. Prognostic stratification analysis based on different lymph node staging systems indicated that a lymph node ratio greater than 0.28 and more than three positive lymph nodes were associated with a high-risk prognosis. Furthermore, postoperative radiotherapy significantly improved lung cancer-specific survival in overall resectable node-positive, stage III, non-small cell lung cancer ( P < 0.05). Notably, survival curve analysis revealed the most pronounced differences in lung cancer-specific survival between the groups receiving postoperative radiotherapy or not in the high-risk prognosis group, particularly among those with a lymph node ratio greater than 0.28, and more than three positive lymph nodes, and lastly the traditional N staging model.
The lymph node ratio classification model demonstrated the highest prognostic prediction performance, with the highest C-index, area under the curve, and the lowest Akaike information criterion, followed by the positive lymph nodes classification model and the traditional N staging model. Prognostic stratification analysis based on different lymph node staging systems indicated that a lymph node ratio greater than 0.28 and more than three positive lymph nodes were associated with a high-risk prognosis. Furthermore, postoperative radiotherapy significantly improved lung cancer-specific survival in overall resectable node-positive, stage III, non-small cell lung cancer ( P < 0.05). Notably, survival curve analysis revealed the most pronounced differences in lung cancer-specific survival between the groups receiving postoperative radiotherapy or not in the high-risk prognosis group, particularly among those with a lymph node ratio greater than 0.28, and more than three positive lymph nodes, and lastly the traditional N staging model.
Conclusion In patients with resectable node-positive, stage III, non-small cell lung cancer, classification according to the lymph node ratio, followed by the positive lymph nodes, may offer superior prognostic prediction capabilities compared to the traditional N staging in addressing prognostic heterogeneity. Additionally, identifying a high-risk prognosis with a lymph node ratio greater than 0.28 appears to be the most effective criterion for selecting candidates who would benefit from postoperative radiotherapy.
In patients with resectable node-positive, stage III, non-small cell lung cancer, classification according to the lymph node ratio, followed by the positive lymph nodes, may offer superior prognostic prediction capabilities compared to the traditional N staging in addressing prognostic heterogeneity. Additionally, identifying a high-risk prognosis with a lymph node ratio greater than 0.28 appears to be the most effective criterion for selecting candidates who would benefit from postoperative radiotherapy.
摘要：
BACKGROUND: Tongxinluo capsule, a formally classical commercial Chinese polyherbal preparation, has been utilized to treat patients with acute myocardial infarction for decades. PURPOSE: This meta-analysis aimed to comprehensively evaluate the clinical outcomes of tongxinluo capsule treated acute myocardial infarction. METHODS: Randomized controlled trials evaluating the effectiveness of tongxinluo capsule alone or in combination with conventional therapy in patients with acute myocardial infarction were identified from eight major databases: Chinese Biomedical Medicine, China National Knowledge Infrastructure, Wanfang Med Database, China Science and Technology Journal Database, PubMed, and Cochrane Central Register of Controlled Trials. In addition, two clinical trial registry platforms (clinicalTrials.gov and the WHO International Clinical Trials) were also searched for relevant studies, with the search extending to all published literature until December 2024. The initial screening and evaluation of the studies were carried out by two independent reviewers who assessed each study according to predefined eligibility criteria. The risk of bias in the research was evaluated using the Cochrane Collaboration's methodology for assessing methodology. Meta-analysis was carried out using RevMan 5.3 software, and publication bias was assessed utilizing StataMP 14.0. The evidence's quality was determined by the Grading of Recommendations Assessment, Development, and Evaluation process. RESULTS: This research included a total of 36 randomized controlled trials with 7002 patients. The meta-analysis revealed that Tongxinluo capsule combined with conventional treatment significantly decreased the 1-month MACCE rate (RR = 0.62, 95% CI 0.47 to 0.81; p = 0.0007), along with the individual risks of 1-month MACCE, including cardiac death (RR = 0.68, 95% CI 0.50 to 0.93; p = 0.02) and myocardial reinfarction (RR = 0.11, 95% CI 0.01 to 0.94; p = 0.04). After 12months of treatment, the MACCE rate (RR = 0.61, 95% CI 0.49 to 0.75; p < 0.00001), cardiac death (RR = 0.69, 95% CI 0.50 to 0.96; p = 0.03), myocardial reinfarction (RR = 0.32, 95% CI 0.13 to 0.75; p = 0.009), and stroke (RR = 0.42, 95% CI 0.20 to 0.87; p = 0.02) were also reduced. The remaining secondary outcomes-1-month stroke (RR = 0.44, 95% CI 0.44 to 1.44; p = 0.18), 12-month (RR = 0.12, 95% CI 0.01 to 2.14; p = 0.15) emergent coronary revascularization, 12-month all-cause mortality (RR = 0.78, 95% CI 0.60 to 1.01; p = 0.06)-showed no differences. Furthermore, the combination of Tongxinluo capsule and conventional therapy increased the incidence of the adverse drug reaction, mainly gastrointestinal discomfort (RR = 1.80, 95% CI 1.14 to 2.84; p = 0.01). However, there were no differences in the liver function levels of aspartate transaminase (SMD = -0.24, 95% CI -0.54 to -0.07; p = 0.12) and alanine aminotransferase (SMD = -0.25, 95% CI -0.55 to 0.05; p = 0.11), or the kidney function levels of blood urea nitrogen (SMD = 0.32, 95% CI -0.21 to 0.86; p = 0.23) and creatinine (SMD = 0.10, 95% CI -0.20 to 0.40; p = 0.52). CONCLUSION: Current data indicates that Tongxinluo capsule, used as an adjuvant treatment, may enhance clinical outcomes for AMI patients at 1- and 12-month. Moreover, it may enhance heart function, regulate lipid peroxidation, and suppress inflammatory levels.
作者：
Ye, Du;Zhu, Junping;Su, Siya;Yu, Yunfeng;Zhang, Jun;...
期刊：
Frontiers in Pharmacology,2025年16:1571767 ISSN：1663-9812
通讯作者：
Yu, R
作者机构：
[Yu, Yunfeng; Xie, Xuejiao; Ye, Du; Zhu, Junping; Yu, Rong; Xiang, Qin; Yin, Yuman; Yu, R] Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.;[Su, Siya] Hunan Univ Chinese Med, Hosp 2, Changsha, Hunan, Peoples R China.;[Zhang, Jun] Hunan Univ Chinese Med, Sch Informat, Changsha, Hunan, Peoples R China.;[Lin, Chuanquan] Guangzhou Univ Chinese Med, Guangzhou, Guangdong, Peoples R China.
通讯机构：
[Yu, R ] H;Hunan Univ Chinese Med, Coll Chinese Med, Changsha, Hunan, Peoples R China.
关键词：
diabetes mellitus;mitophagy;natural small molecules;review;signaling pathway
摘要：
Diabetes mellitus (DM) is a chronic metabolic disorder marked by sustained hyperglycemia. These disturbances contribute to extensive damage across various tissues and organs, giving rise to severe complications such as vision loss, kidney failure, amputations, and higher morbidity and mortality rates. Furthermore, DM imposes a substantial economic and emotional burden on patients, families, and healthcare systems. Mitophagy, a selective process that targets the clearance of damaged or dysfunctional mitochondria, is pivotal for sustaining cellular homeostasis through mitochondrial turnover and recycling. Emerging evidence indicates that dysfunctional mitophagy acts as a key pathogenic driver in the pathogenesis of DM and its associated complications. Natural small molecules are particularly attractive in this regard, offering advantages such as low toxicity, favorable pharmacokinetic profiles, excellent biocompatibility, and a broad range of biochemical activities. This review systematically evaluates the mechanistic roles of natural small molecules-including ginsenosides, resveratrol, and berberine-in enhancing mitophagy and restoring mitochondrial homeostasis via activation of core signaling pathways (e.g., PINK1/Parkin, BNIP3/NIX, and FUNDC1). These pathways collectively ameliorate pathological hallmarks of DM, such as oxidative stress, chronic inflammation, and insulin resistance. Furthermore, the integration of nanotechnology with these compounds optimizes their bioavailability and tissue-specific targeting, thereby establishing a transformative therapeutic platform for DM management. Current evidence demonstrates that mitophagy modulation by natural small molecules not only offers novel therapeutic strategies for DM and its chronic complications but also advances the mechanistic foundation for future drug development targeting metabolic disorders.
摘要：
BACKGROUND: Postprandial distress syndrome (PDS) is the prominent subtype in patients with functional dyspepsia (FD) and currently lacks a satisfactory treatment. Acupuncture has become a promising alternative and complementary therapy for managing FD. However, high-level clinical evidence supporting the use of acupuncture for FD is limited. METHODS: This study is a multicentre, double-dummy, single-blind, randomized, active-controlled trial. Two hundred and one eligible participants will be randomly assigned into three groups: a verum acupuncture plus placebo group, an itopride plus sham acupuncture group, and a sham acupuncture plus placebo group. This study consists of a 1-week screening period, a 4-week treatment period, and a 12-week follow-up period. During the intervention period, participants will receive 12 sessions of verum or sham acupuncture treatment (one session per day, three sessions per week, for 4 weeks) along with 50 mg itopride tablets or 50 mg itopride placebo tablets 3 times a day for 20 days (5 continual days a week for 4 weeks). The response rate (patients who had adequate relief of gastric symptoms will be considered positive responders) and the elimination rate of cardinal symptoms (postprandial fullness and early satiation) are the primary indicators to evaluate the overall acupuncture effect for PDS. Secondary outcome measures will include the Nepean Dyspepsia Symptom Index (NDSI), the short form-Nepean Dyspepsia Life Quality Index (SF-NDLQI), the Hospital Anxiety and Depression Scale (HADS), and related hormone concentrations. Participants' expectations toward acupuncture treatment will also be assessed, and adverse events will be recorded for safety assessment. All analyses will adhere to an intention-to-treat principle. DISCUSSION: In conclusion, this trial will determine the efficacy and safety of acupuncture for PDS and provide more high-level evidence to support its application in treating FD. TRIAL REGISTRATION: Identifier [ITMCTR2024000510].
期刊：
International Journal of Pharmaceutics,2025年676:125530 ISSN：0378-5173
通讯作者：
Wang, C
作者机构：
[Han, Zhouxin; Yuan, Jiao; Wang, C; Wang, Shiyu; Wang, Chang; Zhang, Jinqing; He, Lingzhi; Zhu, Qin] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Dermatol, Domest First Class Discipline Construct Project Ch, Changsha 410005, Peoples R China.;[Zhou, Lingyue] Hunan Univ Chinese Med, Inst Innovat & Appl Res, 300 Bachelor Rd, Changsha 410208, Peoples R China.
通讯机构：
[Wang, C ] H;Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Dermatol, Domest First Class Discipline Construct Project Ch, Changsha 410005, Peoples R China.
摘要：
Psoriasis is a chronic inflammatory skin condition with high oxidative stress and immune dysregulation. Current therapies are limited by side effects and inefficacy over long-term use, highlighting the need for targeted treatments. This study investigates a reactive oxygen species (ROS)-responsive hydrogel (HP)-dissolving microneedle system, loaded with Momordin Ic, designed for controlled, site-specific release to alleviate psoriasis. We hypothesized that this system could target psoriatic inflammation by reducing pro-inflammatory cytokines and oxidative stress. The Franz diffusion cell assay confirmed that Momordin Ic release was accelerated under elevated ROS conditions, demonstrating the stimuli-responsive nature of the hydrogel microneedles system. An imiquimod (IMQ)-induced psoriasis-like mouse model was used to evaluate the therapeutic efficacy of the Momordin Ic/HP microneedle system. The HP microneedles exhibited controlled Momordin Ic release under elevated ROS, and in vitro studies showed reduced ROS levels and blocked hyperproliferation in HaCaT keratinocytes. In vivo , the microneedle treatment alleviated psoriasis symptoms, reducing erythema, scaling, and epidermal thickness while downregulating inflammatory markers (IL-17A, TNF-α). These findings suggest that Momordin Ic/HP microneedles provide a promising therapeutic approach to treating inflammatory skin diseases like psoriasis by combining anti-inflammatory and ROS-scavenging functions.
Psoriasis is a chronic inflammatory skin condition with high oxidative stress and immune dysregulation. Current therapies are limited by side effects and inefficacy over long-term use, highlighting the need for targeted treatments. This study investigates a reactive oxygen species (ROS)-responsive hydrogel (HP)-dissolving microneedle system, loaded with Momordin Ic, designed for controlled, site-specific release to alleviate psoriasis. We hypothesized that this system could target psoriatic inflammation by reducing pro-inflammatory cytokines and oxidative stress. The Franz diffusion cell assay confirmed that Momordin Ic release was accelerated under elevated ROS conditions, demonstrating the stimuli-responsive nature of the hydrogel microneedles system. An imiquimod (IMQ)-induced psoriasis-like mouse model was used to evaluate the therapeutic efficacy of the Momordin Ic/HP microneedle system. The HP microneedles exhibited controlled Momordin Ic release under elevated ROS, and in vitro studies showed reduced ROS levels and blocked hyperproliferation in HaCaT keratinocytes. In vivo , the microneedle treatment alleviated psoriasis symptoms, reducing erythema, scaling, and epidermal thickness while downregulating inflammatory markers (IL-17A, TNF-α). These findings suggest that Momordin Ic/HP microneedles provide a promising therapeutic approach to treating inflammatory skin diseases like psoriasis by combining anti-inflammatory and ROS-scavenging functions.
摘要：
PURPOSE: To explore the related mechanisms of electroacupuncture at the "Siguan" acupoints in treating post-stroke depression (PSD). METHODS: Fifty male SD rats were randomly divided into the blank group, stroke group, PSD group, Siguan group and Bifidobacterium group, with 10 rats in each group. The Siguan group was given electroacupuncture at the bilateral "Hegu" (LI4) and "Taichong" (LR3), using the disperse-dense wave with a frequency of 2Hz/10Hz for 30minutes each time. The changes in depressive behaviors of rats were observed according to the sucrose consumption and the scores of the open field test of rats in each group. The gene data of the intestinal flora of rats in each group were extracted to analyze the differences in the diversity, composition structure and function of the intestinal flora among different groups. RESULTS: The scores of the depression behavior indexes of the PSD rats in the Siguan group were higher than those in the PSD group. Meanwhile, there were disorders in the structure and function of the intestinal flora of the PSD rats. Compared with the PSD group, both the Siguan group and the Bifidobacterium group had increased contents of beneficial bacteria (Firmicutes and Clostridia) and decreased contents of pathogenic bacteria (Bacteroidetes and Bacteroidia). Compared with the PSD group, the intestinal flora structures of the Siguan group and the Bifidobacterium group were closer to those of normal rats. The abundance of the "Metabolism of Cofactors and Vitamins" functional pathway of the intestinal flora in the Siguan group was lower than that in the PSD group. CONCLUSION: Electroacupuncture at the "Siguan" acupoints can improve the depression-like behaviors of PSD rats by up-regulating intestinal probiotics, down-regulating pathogenic bacteria, restoring the physiological structure of the intestinal flora and regulating the function of the intestinal flora.
摘要：
In recent years, the association between systemic immune-inflammation index (SII) and the prognosis of patients with colorectal cancer (CRC) has remained a topic of considerable debate. To address this, the present study was carried out to investigate the prognostic significance of SII in CRC. Databases including PubMed, Embase, the Cochrane Central Register of Controlled Trials (CENTRAL), and Web of Science were scrutinized up to March 27, 2024. The relationship between pre- and post-treatment SII levels and the prognosis of CRC was evaluated. Following literature screening, quality assessment, and extraction of outcome measures, a meta-analysis was conducted using Stata. Publication bias was assessed by funnel plots and Egger’s test. A total of 27 studies were included in the analysis. Pooled results demonstrated that a high SII level was associated with poor overall survival (OS, HR = 1.78, 95% CI = 1.40–2.26), progression-free survival (PFS, HR = 1.80, 95% CI = 1.26–2.56), disease-free survival (DFS, HR = 1.91, 95% CI = 1.43–2.56), and recurrence-free survival (RFS, HR = 3.29, 95% CI = 1.58–6.88). Notably, the association between pre-treatment SII and OS, PFS, and DFS was stronger than that observed for post-treatment SII, indicating that treatment may attenuate the predictive valueof SII for survival outcomes. Additionally, elevated SII was correlated with poor tumor differentiation, tumor location in the rectum, and larger tumor size ≥ 5 cm. Our meta-analysis suggested that a high SII is a predictor of poor prognosis in CRC patients. High SII levels were strongly correlated with inferior OS, PFS, DFS, and RFS. The relationship between SII and survival outcomes was attenuated post-treatment compared to pre-treatment. Additionally, elevated SII was correlated with clinicopathological factors in CRC patients. These findings suggest that SII can serve as an independent prognostic indicator for CRC.
关键词：
lip-mandible preservation;lip-split mandibulotomy;mandibular lingual release;meta-analysis;oral and oropharyngeal cancer
摘要：
Background: Many studies have compared lip-splitting mandibulotomy (LSM) and lip-mandible preservation (LMP) techniques in oral and oropharyngeal cancer (OOPC) patients with inconsistent conclusions. Evidence-based recommendations for the optimal surgical approach for treating OOPC are lacking. Methods: The Cochrane Library, Pubmed, Embase, Web of Science, WAN-FANG, CQVIP, and China National Knowledge Infrastructure were systematically searched to identify studies that compared LSM versus LMP for OOPC. An additional search of the gray literature was performed using Google Scholar, OpenGrey and ProQuest Dissertations & Theses Global. Survival rate, recurrence rate, surgical margin, perioperative outcomes, postoperative complications and functional status were assessed. The standard mean difference (SMD) and odds ratio (OR) with a 95% CI were pooled using fixed-effect or random-effect models. Results: Four randomized controlled trials, five case-control studies, and twenty cohort studies including a total of 2622 patients were identified. The LSM approach significantly increased postoperative complications such as mandibular osteomyelitis/osteoradionecrosis (OR = 4.57; 95% CI = 1.20-17.39; P = 0.026), fistula (OR = 1.5; 95% CI = 1.05-2.15; P = 0.027), and flap infection (OR = 2.96; 95% CI = 1.49-5.87; P = 0.002), while LMP improved facial appearance (SMD = −0.65; 95% CI = −1.05 to −0.25; P = 0.002). Meta-analyses showed no significant difference in survival rate (OR = 1.07; 95% CI = 0.83-1.38; P = 0.59), total recurrence (OR = 1.15; 95% CI = 0.87-1.52; P = 0.325), local recurrence (OR = 1.39; 95% CI = 0.88-2.19; P = 0.163), operation duration (SMD = 0.19; 95% CI = −0.75 to 1.13; P = 0.688), length of hospital stay (SMD = 0.48; 95% CI = −0.27 to 1.22; P = 0.208), volume of blood loss (SMD = 0.43; 95% CI = −0.17 to 1.03; P = 0.156), surgical margin (OR = 1.01; 95% CI = 0.72-1.41; P = 0.947), hematoma/seroma (OR = 1.01; 95% CI = 0.46-2.25; P = 0.972), wound infection (OR = 1.28; 95% CI = 0.92-1.79; P = 0.145), swallowing (SMD = −0.33; 95% CI = −0.91 to 0.24; P = 0.428) and speech (SMD = −0.14; 95% CI = −0.44 to 0.17; P = 0.381) between the LSM and LMP groups. Conclusion: These findings suggest that LMP may be a safe and efficient alternative to LSM for treating OOPC patients with decreased mandibular osteomyelitis/osteoradionecrosis, fistula, flap infection, and a better aesthetic outcome.
期刊：
CURRENT COMPUTER-AIDED DRUG DESIGN,2025年21(4):534-548 ISSN：1573-4099
通讯作者：
Zeng, Jingqi;Wang, F
作者机构：
[Ma, Huaiyu; Xu, Haoran; Wu, Jie; Wu, Ruizhe; Wang, F; Zeng, Jingqi; Zeng, JQ; Chen, Weixing; Wang, Fan; Jin, Hui] Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Orthoped, Changsha 410005, Peoples R China.;[Wu, Jie; Wu, Ruizhe] Hunan Univ Chinese Med, Grad Sch, Changsha 410208, Peoples R China.;[Li, Linghui] China Acad Chinese Med Sci, Wangjing Hosp, Dept Sports Med, Beijing 100102, Peoples R China.
通讯机构：
[Wang, F ; Zeng, JQ] H;Hunan Univ Chinese Med, Affiliated Hosp 2, Dept Orthoped, Changsha 410005, Peoples R China.
关键词：
Intervertebral disc degeneration;Qing’e Pill;in vivo experiments;molecular mechanisms;molecular docking;network pharmacology.
摘要：
Objective The Qing’e Pill (QEP) is widely used to alleviate low back pain and sciatica caused by Intervertebral Disc Degeneration (IDD). However, its active components, key targets, and molecular mechanisms are not fully understood. The aim of this study is to elucidate the molecular mechanisms through which the QEP improves IDD using database mining techniques.
The Qing’e Pill (QEP) is widely used to alleviate low back pain and sciatica caused by Intervertebral Disc Degeneration (IDD). However, its active components, key targets, and molecular mechanisms are not fully understood. The aim of this study is to elucidate the molecular mechanisms through which the QEP improves IDD using database mining techniques.
Methods Active components and candidate targets of the QEP were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and the Bioinformatics Analysis Tool for Molecular Mechanisms of Traditional Chinese Medicine. IDD-related targets were obtained from the GeneCards database, and liver- and kidney-specific genes were retrieved from the BioGPS database. The intersection of these candidate targets was analyzed to identify potential targets for the QEP in IDD. A protein-protein interaction network analysis was performed using STRING and Cytoscape 3.7.2 software. Core targets were further analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Molecular docking was used to assess the binding affinity of active components to candidate targets, and animal experiments were conducted for validation.
Active components and candidate targets of the QEP were identified using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and the Bioinformatics Analysis Tool for Molecular Mechanisms of Traditional Chinese Medicine. IDD-related targets were obtained from the GeneCards database, and liver- and kidney-specific genes were retrieved from the BioGPS database. The intersection of these candidate targets was analyzed to identify potential targets for the QEP in IDD. A protein-protein interaction network analysis was performed using STRING and Cytoscape 3.7.2 software. Core targets were further analyzed through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses. Molecular docking was used to assess the binding affinity of active components to candidate targets, and animal experiments were conducted for validation.
Results We identified 65 potentially active components of the QEP that corresponded to 1,093 candidate targets, 2,108 IDD-related targets, and 1,113 liver- and kidney-specific genes. Key components included quercetin, berberine, isorhamnetin, and emodin. The primary candidate targets were Wnt5A, CTNNB1, IL-1β, MAPK14, MMP9, and MMP3. The GO and KEGG analyses revealed the involvement of these targets in Wnt signaling, TNF signaling, Wnt receptor activation, Frizzled binding, and Wnt-protein interactions. Molecular docking showed strong binding between these components and their targets. Animal experiments demonstrated that the QEP treatment significantly reduced the expression of Wnt5A, CTNNB1, IL-1β, MAPK14, MMP9, and MMP3 at high, medium, and low doses compared with the model group.
We identified 65 potentially active components of the QEP that corresponded to 1,093 candidate targets, 2,108 IDD-related targets, and 1,113 liver- and kidney-specific genes. Key components included quercetin, berberine, isorhamnetin, and emodin. The primary candidate targets were Wnt5A, CTNNB1, IL-1β, MAPK14, MMP9, and MMP3. The GO and KEGG analyses revealed the involvement of these targets in Wnt signaling, TNF signaling, Wnt receptor activation, Frizzled binding, and Wnt-protein interactions. Molecular docking showed strong binding between these components and their targets. Animal experiments demonstrated that the QEP treatment significantly reduced the expression of Wnt5A, CTNNB1, IL-1β, MAPK14, MMP9, and MMP3 at high, medium, and low doses compared with the model group.
Conclusion The QEP alleviated IDD by modulating the Wnt/MAPK/MMP signaling pathways and reducing the release and activation of key factors.
The QEP alleviated IDD by modulating the Wnt/MAPK/MMP signaling pathways and reducing the release and activation of key factors.
摘要：
We express our profound gratitude for the insightful feedback provided by our peers following the publication of our article. We also appreciate the opportunity to respond to these comments. Our responses are outlined as follows:
期刊：
Frontiers in Medicine,2025年12:1541364 ISSN：2296-858X
通讯作者：
Lei, MS;Xiao, Y
作者机构：
[Tan, Gan; Lei, Mingsheng; Huang, Jianliang; Zhang, Yurou; Wang, Ling; Xia, Mingkai] Hunan Normal Univ, Dept Pulm & Crit Care Med, Zhangjiajie Hosp, Zhangjiajie, Hunan, Peoples R China.;[Lei, Mingsheng; Huang, Jianliang; Wang, Ling] Zhangjiajie Coll, Biomed Res Inst, Zhangjiajie, Hunan, Peoples R China.;[Kang, Zhen] Hunan Univ Chinese Med, Affiliated Hosp 2, Changsha, Hunan, Peoples R China.;[Xiao, Y; Xiao, Yun] Changsha Cent Hosp, Dept Pulm & Crit Care Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Lei, MS ] H;[Xiao, Y ] C;Hunan Normal Univ, Dept Pulm & Crit Care Med, Zhangjiajie Hosp, Zhangjiajie, Hunan, Peoples R China.;Zhangjiajie Coll, Biomed Res Inst, Zhangjiajie, Hunan, Peoples R China.;Changsha Cent Hosp, Dept Pulm & Crit Care Med, Changsha, Hunan, Peoples R China.
摘要：
BACKGROUND: Pulmonary fibrosis is a fatal disease characterized by progressive scarring of lung tissue, with a complex pathogenesis and limited therapeutic options. The identification of robust biomarkers is critical for addressing key clinical challenges, including delayed diagnosis and poor prognostic assessment. METHODS: This study systematically analyzes global research trends and emerging hotspots in pulmonary fibrosis biomarkers by examining literature from 2001 to 2024 indexed in the Web of Science Core Collection. Utilizing a suite of bibliometric tools including VOSviewer, CiteSpace, Bibliometrix, Scimago Graphica, and OriginPro 2021, this work provides the first comprehensive insight into the evolving landscape of biomarker research in pulmonary fibrosis. RESULTS: This study included a total of 2,519 articles and reviews related to pulmonary fibrosis biomarkers. Since 2005, publication trends in this field have steadily increased. Research on pulmonary fibrosis biomarkers has involved 71 countries, 3,036 institutions, 760 journals, and over 14,000 researchers. China produced the highest number of publications (n = 535, 21.2%, TLCS = 459), followed by the United States (n = 529, 21%, TLCS = 3,527) and Japan (n = 270, 10.7%, TLCS = 1,279), with the United States exerting the greatest influence. The UNIVERSITY OF CALIFORNIA SYSTEM (n = 164) and HARVARD UNIVERSITY (n = 141) contributed the largest bodies of work. The most prolific authors in this domain are BARGAGLI E (n = 45), MAHER TM (n = 42), and MARTINEZ FJ (n = 32). The AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE is widely regarded as the leading journal in this field. In recent years, research has increasingly focused on macrophages, computed tomography, and Muc5b promoter polymorphism, among other areas. The concept of "double blind" reflects the translational trend of biomarkers toward clinical applications, particularly their potential utility in acute exacerbations of pulmonary fibrosis, interstitial pulmonary fibrosis, cystic pulmonary fibrosis, and radiation-induced pulmonary fibrosis. CONCLUSION: The clinical application of gene and imaging biomarkers achieved through the integration of multiple parameters and multi-omics fusion represents a promising future trend and emerging hotspot in pulmonary fibrosis biomarker research.
摘要：
Background The aim of this study was to investigate the regulation of Salvianolic acid A (SAA) on the chondrogenic differentiation of bone mesenchymal stem cells (BMSCs), and its effect on cartilage repair in knee osteoarthritis (KOA) model rats and the action mechanism.
The aim of this study was to investigate the regulation of Salvianolic acid A (SAA) on the chondrogenic differentiation of bone mesenchymal stem cells (BMSCs), and its effect on cartilage repair in knee osteoarthritis (KOA) model rats and the action mechanism.
Methods Immunohistochemistry was performed to detect collagen type II (COL2A1), MMP13 and caspase-3 (CASP3) expression in cartilage tissues, and Safranin-O/Fast Green staining for cartilage damage. Alcian blue staining was performed to measure chondrogenic differentiation of BMSCs. Chondrocyte apoptosis was detected by using flow cytometry.
Immunohistochemistry was performed to detect collagen type II (COL2A1), MMP13 and caspase-3 (CASP3) expression in cartilage tissues, and Safranin-O/Fast Green staining for cartilage damage. Alcian blue staining was performed to measure chondrogenic differentiation of BMSCs. Chondrocyte apoptosis was detected by using flow cytometry.
Results SAA treatment significantly attenuated cartilage damage in KOA model rats in a dose-dependent manner, and inhibited chondrocyte apoptosis induced by IL-1β in a dose-dependent manner. Moreover, SAA treatment promoted chondrogenesis-related proteins (COL2A1 and Aggrecan) expression and inhibited catabolism-related proteins (MMP13 and MMP3) expression both in the cartilage tissues from KOA model rat and in the IL-1β-treated chondrocytes. WD repeat domain 5 (WDR5) was a downstream target of SAA, and it facilitated chondrogenic differentiation of BMSCs derived from KOA model rats (KOA-BMSCs). Importantly, the inhibition of SAA treatment to the apoptosis and catabolism of chondrocyte and the promotion of SAA treatment to chondrogenic differentiation of KOA-BMSCs were rescued by silencing WDR5.
SAA treatment significantly attenuated cartilage damage in KOA model rats in a dose-dependent manner, and inhibited chondrocyte apoptosis induced by IL-1β in a dose-dependent manner. Moreover, SAA treatment promoted chondrogenesis-related proteins (COL2A1 and Aggrecan) expression and inhibited catabolism-related proteins (MMP13 and MMP3) expression both in the cartilage tissues from KOA model rat and in the IL-1β-treated chondrocytes. WD repeat domain 5 (WDR5) was a downstream target of SAA, and it facilitated chondrogenic differentiation of BMSCs derived from KOA model rats (KOA-BMSCs). Importantly, the inhibition of SAA treatment to the apoptosis and catabolism of chondrocyte and the promotion of SAA treatment to chondrogenic differentiation of KOA-BMSCs were rescued by silencing WDR5.
Conclusion Overall, SAA treatment could facilitate cartilage repair via inhibiting the apoptosis and catabolism of chondrocyte and promoting chondrogenic differentiation of KOA-BMSCs by promoting WDR5 expression. Our data suggested that SAA may a potential drug for the treatment of KOA.
Overall, SAA treatment could facilitate cartilage repair via inhibiting the apoptosis and catabolism of chondrocyte and promoting chondrogenic differentiation of KOA-BMSCs by promoting WDR5 expression. Our data suggested that SAA may a potential drug for the treatment of KOA.
作者：
Ye, Hai-min;Li, Zhuo-yan;Zhang, Peng;Kang, Zhen;Zhou, De-sheng
期刊：
中国结合医学杂志,2025年31(2):183-192 ISSN：1672-0415
通讯作者：
Zhou, DS
作者机构：
[Ye, Hai-min] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Peoples R China.;[Li, Zhuo-yan; Ye, Hai-min] Hunan Univ Chinese Med, Affiliated Hosp 2, Neurol Dept, Changsha 410005, Peoples R China.;[Zhang, Peng; Kang, Zhen] Hunan Univ Chinese Med, Affiliated Hosp 2, Acupuncture & Moxibust Massage Rehabil Dept, Changsha 410005, Peoples R China.;[Zhou, De-sheng; Zhou, DS] Hunan Univ Chinese Med, Hosp 1, Neurol Dept, Changsha 410007, Peoples R China.
通讯机构：
[Zhou, DS ] H;Hunan Univ Chinese Med, Hosp 1, Neurol Dept, Changsha 410007, Peoples R China.
关键词：
electroacupuncture;intestinal flora;metabolites of intestinal flora;neuroinflammation;nervous system disease;review
摘要：
Neuroinflammatory responses play an important role in the pathogenesis of various diseases, particularly those affecting the central nervous system. Inhibition of neuroinflammation is a crucial therapeutic strategy for the management of central nervous system disorders. The intestinal microbial-gut-brain axis serves as a key regulatory pathway that modulates neuroinflammatory processes. Intestinal flora metabolites such as short-chain fatty acids, indoles and their derivatives, lipopolysaccharides, trimethylamine oxide, and secondary bile acids exert direct or indirect effects on neuroinflammation. Studies have shown that electroacupuncture (EA) modulates the composition of the intestinal microbiota and its metabolites, while also suppressing neuroinflammation by targeting the TLR4/NF- κ B, NLRP3/caspase-1, and microglial cell M2-type transformation pathways. This review discusses the mechanisms by which EA regulates neuroinflammation via intestinal microbiota and its metabolites, providing information and a foundation for further investigation of the precise therapeutic mechanisms of EA in neurological disorders.
