期刊：
Medical Science Monitor,2019年25:5389-5400 ISSN：1643-3750
通讯作者：
Luo, Yinhe;Wang, Mengqing
作者机构：
[Zhang, Xin; Chen, Xingyu; Ding, Yi; Yang, Jingyi; Sun, Le; Li, Yan; Huang, Kailing; Chen, Yingying; Jiao, Luojia; Huang, Ting] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Luo, Yinhe] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Hunan, Peoples R China.;[Wang, Mengqing] Hunan Univ Chinese Med, Domest Class Discipline Construc Project Chinese, Changsha, Hunan, Peoples R China.
通讯机构：
[Luo, Yinhe; Wang, Mengqing] H;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Domest Class Discipline Construc Project Chinese, Changsha, Hunan, Peoples R China.
摘要：
Background: Dendritic cell autophagy plays a pivotal role in asthma. Wuhu decoction can significantly improve respiratory syncytial virus (RSV) bronchiolitis and delay its development into asthma. The aim of the present study was to explore the therapeutic effect and mechanism of Wuhu decoction on RSV -induced asthma in mice. Material/Methods: Establishment of asthmatic mice model was induced by RSV. Hematoxylin-eosin staining, periodic acid-Schiff (PAS) staining, and Masson trichrome staining were performed to observe pathological changes in the lungs. The levels of CD4(+) T, CD8(+) T, and CD4(+) CD25(+) T in blood were analyzed by flow cytometry. The contents of interleukin (IL)-4, interferon-gamma (IFN-gamma), IL-10, and IL-13 in serum were measured by enzyme-linked immunosorbent assay (ELISA). The number of autophagosomes in dendritic cells (DCs) of lung tissue was observed by transmission electron microscope. The DCs of lung tissue were isolated by magnetic bead sorting. The levels of LC3-II, Beclin-1, and LC3-I in DCs and MMP-9, TIMP-1, AMPK, p-AMPK, ULK1, and LK1 expression in lung tissues were detected by western blot. Real-time polymerase chain reaction (PCR) detected the expression of AMPK and ULK1 genes. Results: Wuhu decoction can effectively alleviate chronic airway inflammation and airway remodeling and reduce airway hyperresponsiveness. Moreover, Wuhu decoction can significantly enhance the level of autophagy in DCs of lung tissue and promote the expression of AMPK and ULK1 in lung tissue. Conclusions: Wuhu decoction may improve the RSV-induced asthmatic symptoms by enhancing autophagy of DCs in lung tissue dependent on the AMPK/ULK1 signaling pathway.
关键词：
miR-34a;Survivin;Platycodin D;Susceptibility;Gastric cancer
摘要：
INTRODUCTION: Platycodin D (PD), a triterpenoid saponin isolated from Platycodon grandiflorum, has a well-known anti-tumor effect in multiple human cancers, including gastric cancer (GC). miR-34a plays an important role in the progression of GC. However, the relationship between miR-34a and the susceptibility of GC cells to PD is still unclear. The aim of our research was to investigate the functions of miR-34a in mediating the susceptibility of GC to PD. METHODS: qPCR was performed to detect the expression level of miR-34a and survivin in GC cells. The expression of survivin, Bcl-2, Bax, and cleaved caspase-3 was analyzed using Western blot. Cell viability was detected by MTT assay, and apoptosis was analyzed via Annexin V-FITC/PI staining followed by flow cy-tometry. The colony formation and scratch-wound assays were applied to assess cell proliferation and migration. Caspase-3/7 activity was detected by a Caspase-Glo(R)3/7 detection kit. The relationship between miR-34a and survivin was determined by dual luciferase reporter gene assay. Finally, a GC xenograft mouse model was used to confirm our findings in vivo. RESULTS: The expression of miR-34a decreased but survivin increased inversely in human GC cells. Survivin is a direct target of miR-34a and may be negatively regulated by miR-34a. PD could inhibit GC cell proliferation and induce apoptosis. Importantly, overexpression miR-34a or suppressing survivin was shown to enhance the susceptibility of GC to PD both in vitro and in vivo. CONCLUSIONS: miR-34a could modulate the susceptibility of GC to PD via targeting survivin, suggesting miR-34a overexpression may serve as a novel strategy to sensitize GC to anti-cancer drugs.
作者机构：
[何业文; 沈智豪; Shen Z.; 高辉; 李平; 黎建宇; 钟泽亚] Department of Radiology, First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;Department of Internal Medicine, The Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine, Changsha, 410006, China;[朱璐] Department of Ultrasonography, Hunan Provincial People's Hospital, First Affiliated Hospital of Hunan Normal University, Changsha, 410005, China;[范阳] GE Healthcare (Shanghai) Co., Ltd., Shanghai, 201203, China
通讯机构：
[Zhu, K.] C;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China
期刊：
Prostaglandins & Other Lipid Mediators,2019年140:1-8 ISSN：1098-8823
通讯作者：
Zou, Guohui;Deng, Changqing
作者机构：
[Chen, Hongtao; Zou, Guohui; Liu, Zhongyong; Xu, Ri; Deng, Peng] Jiangxi Univ Tradit Chinese Med, Affiliated Hosp, Nanchang 330006, Jiangxi, Peoples R China.;[Zhu, Jinhua] Jiangxi Univ Tradit Chinese Med, Nanchang 330004, Jiangxi, Peoples R China.;[Wu, Lu] Hunan Univ Tradit Chinese Med, Affiliated Tradit & Western Med Hosp 2, Liuyang 410300, Peoples R China.;[Deng, Changqing] Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Zou, Guohui] J;[Deng, Changqing] H;Jiangxi Univ Tradit Chinese Med, Affiliated Hosp, Nanchang 330006, Jiangxi, Peoples R China.;Hunan Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.
关键词：
*Detoxification and activating blood circulation decoction;*Inflammatory reaction;*PCI operation;*Restenosis;*TLR4/NF-kappaB;*VSMCs proliferation
摘要：
Restenosis is a major problem after percutaneous coronary intervention (PCI) treatment. Inflammation is one of the major core mechanisms involved in the occurrence of restenosis, and plays an important role in intimal hyperplasia. Detoxification and activating blood circulation decoction (DABCD) is a traditional Chinese medicine that is used in the treatment and prevention of atherosclerotic and inflammatory diseases. Our previous studies demonstrated that DABCD-mediated cardioprotection involves anti-inflammatory mechanisms and could be developed as a novel drug for the treatment of vascular smooth muscle cell (VSMC) proliferation and aortic restenosis. A rat model of postoperative restenosis after PCI was generated by balloon injury to determine the protective effects and potential mechanisms of DABCD. The injured segments of aortae were collected on days 14 and 28 after the operation to observe the morphological changes in the vascular structure and measure the proportion of inflammatory factors in plasma and vascular tissues, as well as test the proliferative activity of VSMCs. The expression of related proteins, namely, Toll-like receptor (TLR) 4 and nuclear factor (NF)-kappaB, in the mechanistic study was clarified by western blot analysis. We tested the hypothesis that the cardioprotective effects of DABCD on aortic restenosis are associated with the inhibition of aortic intimal hyperplasia in this model. Our results showed that DABCD has protective effect on rat aortic restenosis and the anti-inflammatory mechanism of DABCD on balloon-induced restenosis in rat may be due to its ability to inhibit TLR4-mediated NF-kappaB signaling pathways. DABCD may be a potential therapeutic agent against restenosis.
