作者机构：
湖南中医药大学药学院, 湖南, 长沙, 410208;中药成药性与制剂制备湖南省重点实验室, 中药成药性与制剂制备湖南省重点实验室, 湖南, 长沙, 410208;湖南中医药大学中医药超分子机理与数理特征化实验室, 湖南, 长沙, 410208;[李海英; 樊启猛; 孟蕾; 贺玉婷; 贺鹏; 王敏存; 周燕子; 周逸群; 潘雪] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM, Changsha, 410208, China;[贺福元] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China, Hunan Provincial Key Laboratory of Drugability and Preparation Modification of TCM, Changsha, 410208, China, Supramolecular Mechanism and Mathematic-Physics Chracterization for Chinese Materia Medica, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Meng, L.; He, F.-Y.] C;College of Pharmacy, China
作者机构：
[Mao, Y.; Yu, Y.; Liang, N.; Wang, X.-J.] Hunan Food and Drug Vocational College, Changsha, 410208, China;[Wang, W.; Liu, Y.; Gong, L.-M.; Xie, Q.; Duan, S.-L.; Muhammad, D.; Li, B.] TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Drug Research Institute, Sino-pakisitan TCM & Ethnomedicine Research International Cooperation Base (Hunan Province), School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 41020, China;Department of Phamacy, Liuzhou Traditional Chinese Medical Hospital Guangxi Zhuang Autonomous Region, Liuzhou, 545026, China;[Lu, S.-Y.] TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Drug Research Institute, Sino-pakisitan TCM & Ethnomedicine Research International Cooperation Base (Hunan Province), School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 41020, China, Department of Phamacy, Liuzhou Traditional Chinese Medical Hospital Guangxi Zhuang Autonomous Region, Liuzhou, 545026, China
通讯机构：
[Wang, W.] T;TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Drug Research Institute, Sino-pakisitan TCM & Ethnomedicine Research International Cooperation Base (Hunan Province), School of Pharmacy, Hunan University of Chinese Medicine, Changsha, China
摘要：
OBJECTIVE: To investigate efficacy of Lidan Tang (LDT) on gallstone induced by high fat diet in mice, and to study its underlying mechanism. METHODS: Mice were fed with high fat diet every day and treated with LDT (9.01 times of human clinic dosage). Mice were randomly divided into 6 groups as control group, gallstone model group (high-fat diet), positive control ursodeoxycholic acid (UDCA) group (80 mg.kg(-1).d(-1) i.g.), LDT low dose group (6 kg/d, i.g.), LDT middle dose group (12 kg/d, i.g.), and LDT high dose group (24 kg/d, i. g.). The whole experiment was lasted for 4 weeks. The levels of ALT, AST, LDH, CHO, HDL-C and LDL-C in serum were measured, the pathological sections were observed by hematoxylin-eosin staining, the activities of antioxidant enzymes were measured by kits, and the proteins related to oxidative stress and lipid transport were detected by Western blot analysis. RESULTS: LDT could significantly reduce the contents of ALT and AST in serum and improve the pathological tissue of liver. LDT could significantly reduce the content of MDA and LPO, and increase the level of GSH and GSH-PX in liver tissue. The data of Western blot showed that LDT had antioxidant effect promoting Keap1/Nrf2 pathway and regulated the process of lipid transport, which was statistically significant. In addition, LDT treatment inhibited the expression of ATP-binding cassette transports ABCG5/8 in liver, and reduced cholesterol transport from the hepatocytes to the gallbladder. CONCLUSION: LDT has protective effect on gallstones induced by high fat diet in mice, which might be based on the protective effect on liver, including enhancing the antioxidant capacity of liver and reducing the production of lipid peroxides. (C) 2020 JTCM. All rights reserved.
关键词：
Ethnobotany;Indigenous medical systems;Ethnobotanical knowledge;Buyi;Medicinal plants
摘要：
The Buyi are a socio-linguistic group in Yunnan Province of southwest China that have a long history of using medicinal plants as part of their indigenous medical system. Given the limited written documentation of the Buyi indigenous medical system, the objective of this paper is to document the medicinal plants of the Buyi and associated traditional knowledge and transmission. Field research was conducted in four villages in Lubuge Township of Luoping County in Yunnan Province using ethnobotanical methodologies including participatory observation, semi-structured interviews, key informant interviews, and focus group discussions to elicit information on medicinal plants. In total, 120 informants (including 15 key informants who are healers) were interviewed. This study found that a total of 121 medicinal plant species belonging to 64 families are used by the Buyi including by local healers to treat different diseases. Among the medicinal plants recorded in this study, 56 species (46%) have not previously been documented in the scientific literature as having medicinal value, highlighting the pressing need for ethnobotanical documentation in indigenous communities. The most frequently used medicinal part was the leaf (24.9% of documented plants), and the most common preparation method was decoction (62.8% of medicinal). Medicinal plants were mainly used to treat rheumatism (12.4% of plants), trauma and injuries (9.6%). The documented plants are also used for other non-medicinal purposes including food, fodder, fencing, and ornamental. In addition, 35 of the medicinal plants are considered poisonous and are used by local Buyi healers for medicine. The traditional Buyi beliefs and practices associated with the documented medicinal plants likely contributes to their conservation in the environments and around Buyi communities. This study further highlights that ethnomedicinal knowledge of the Buyi is at risk of disappearing due to increased introduction and use of modern medicine in Buyi communities, livelihood changes, rapid modernization, and urbanization. Research, policy, and community programs are urgently needed to conserve the biocultural diversity associated with the Buyi medical system including ethnobotanical knowledge towards supporting both environmental and human wellbeing. Copyright (C) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
作者机构：
湖南中医药大学药学院,湖南 长沙 410208;中国医学科学院药物研究所神经科学中心,天然药物活性物质与功能国家重点实验室,北京 100050;[张钊; 楚世峰] State Key Lab of Bioaclive Substances and Functions of Natural Medicine, Neuroscience Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, 100050, China;[彭也; 陈乃宏] College of Pharmacy, Ниnaп University of Chinese Medicine, Changsha, 410208, China, State Key Lab of Bioaclive Substances and Functions of Natural Medicine, Neuroscience Center, Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing, 100050, China
作者机构：
[Yunshan H.; Huaying H.] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[Yawei L.; Zhoujin T.] School of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
