摘要：
Neuroinflammation contributes to the occurrence and development of epilepsy. However, several inflammatory factors that are important for facilitating the diagnosis to reduce or prevent seizures need to be further studied. This study is aimed to explore serum levels of matrix metalloproteinase-9 (MMP-9), interleukin-6 (IL-6), hypersensitive C-reactive protein (hs-CRP), and homocysteine (HCY) in epilepsy patients and the relationship of them with epilepsy. Epilepsy patients (n = 101) in the Second Xiangya Hospital from January 2017 to August 2018 were allocated to the epilepsy groups, which were divided into idiopathic epilepsy group (n = 43) and symptomatic epilepsy group (n = 58) according to the pathogeny. Healthy individuals (n = 50) were allocated to the control group. The concentrations of serum MMP-9, IL-6, hs-CRP, and HCY in all samples were detected by enzyme-linked immunosorbent assay, chemiluminescence method, latex-enhanced immunoturbidimetry, and enzyme circulation method. The levels of serum MMP-9, IL-6, hs-CRP, and HCY in epilepsy patients were higher than those in the control group (P < 0.05, P < 0.01, P < 0.01, and P < 0.01, respectively). The levels of serum MMP-9, IL-6, hs-CRP, and HCY in the symptomatic epilepsy group were higher than those in the control group (P < 0.01 or P < 0.05, respectively). The levels of serum MMP-9, IL-6, and hs-CRP in idiopathic epilepsy patients were higher than those in the control group (P < 0.01 or P < 0.05, respectively). The serum HCY level in the idiopathic epilepsy group was lower than that in the symptomatic epilepsy group (P < 0.01). MMP-9, IL-6, hs-CRP, and HCY may be recommended as the state biomarker to distinguish etiology of epilepsy. We hope our study could provide help in some ways for clinical diagnosis and treatment.
摘要：
Introduction It is well-known that altered hypothalamus-pituitary-adrenal (HPA) axis process has an important role in the neurodegenerative process in schizophrenia (SZ). However, this neurodegenerative mechanism has not been clarified in SZ. Therefore, the main purpose of this study was to determine HPA axis damage in the first-episode, unmedicated schizophrenia (FES) patients and chronic schizophrenia (CSZ) patients in comparison with healthy controls (HC) by means of quantitative analysis of the peripheral blood mRNA expression of glucocorticoid receptor (GR), GR transcripts containing exons 1B (GR-1B), and neuron specific enolase (NSE) genes and serum cortisol and NSE, a specific serum marker for neuronal damage. Methods In the present study, 43 FES patients, 39 CSZ, and 47 HC were included. The peripheral blood mRNA expressions for GR, GR-1B, and NSE genes were determined by real-time quantitative polymerase chain reaction (RT-qPCR). Serum cortisol and NSE were analyzed by electrochemiluminescence immunoassay technique. Results Levels of GR mRNA were significantly lower in FES and CSZ than that in HC. The expression of GR-1B mRNA was significantly decreased in CSZ when compared with that in FES. Levels of NSE mRNA were significantly lower in CSZ than that in FES patients or HC patients. CSZ patients showed significantly lower cortisol concentrations than FES and HC patients. FES patients showed significantly higher NSE concentrations than CSZ and HC. Conclusion Our findings support that there is disrupted HPA axis system in the SZ and suggest that CSZ patients suffer a greater HPA axis damage than FES patients. Our research implicated underlying GR mRNA dysregulation in SZ and the potential importance of the functional GR-1B transcription in CSZ.
期刊：
Frontiers in Pharmacology,2020年11:527711 ISSN：1663-9812
通讯作者：
Deng, Meichun
作者机构：
[Chen, Gong; Wu, Wenfang; Deng, Meichun; Luo, Qianxuan; Wu, Ting; Kang, Shuntong] Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha, Peoples R China.;[Chen, Gong; Wu, Wenfang; Deng, Meichun; Luo, Qianxuan; Wu, Ting; Kang, Shuntong] Cent South Univ, Sch Life Sci, Hunan Prov Key Lab Basic & Appl Hematol, Changsha, Peoples R China.;[Deng, Meichun; Luo, Qianxuan] Cent South Univ, Sch Life Sci, Hunan Key Lab Anim Models Human Dis, Changsha, Peoples R China.;[Deng, Meichun; Luo, Qianxuan] Cent South Univ, Sch Life Sci, Hunan Key Lab Med Genet, Changsha, Peoples R China.;[Jiang, Liping; Wu, Ting; Kang, Shuntong] Cent South Univ, Xiangya Sch Med, Changsha, Peoples R China.
通讯机构：
[Deng, Meichun] C;Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Prov Key Lab Basic & Appl Hematol, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Key Lab Anim Models Human Dis, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Key Lab Med Genet, Changsha, Peoples R China.
摘要：
Voltage-gated sodium channels (VGSCs), which are abnormally expressed in various types of cancers such as breast cancer, prostate cancer, lung cancer, and cervical cancer, are involved in the metastatic process of invasion and migration. Nav1.5 is a pore-forming alpha subunit of VGSC encoded bySCN5A. Various studies have demonstrated that Nav1.5, often as its neonatal splice form, is highly expressed in metastatic breast cancer cells. Abnormal activation and expression of Nav1.5 trigger a variety of cellular mechanisms, including changing H(+)efflux, promoting epithelial-to-mesenchymal transition (EMT) and the expression of cysteine cathepsin, to potentiate the metastasis and invasiveness of breast cancer cellsin vitroandin vivo. Here, we systematically review the latest available data on the pro-metastatic effect of Nav1.5 and its underlying mechanisms in breast cancer. We summarize the factors affecting Nav1.5 expression in breast cancer cells, and discuss the potential of Nav1.5 blockers serving as candidates for breast cancer treatment.
作者机构：
[Shi, Xiaoliu; Yang, Yuan; Huang, Hui; Tang, Haiyan; Zhao, Chenyu] Cent South Univ, Xiangya Hosp 2, Dept Med Genet, Changsha, Hunan, Peoples R China.;[Zhao, Chenyu] Cent South Univ, Xiangya Hosp 2, Dept Gastroenterol, Changsha, Hunan, Peoples R China.;[Tang, DongFang; Zhou, Xi] Hunan Normal Univ, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Yang, Yuan] Peking Univ Canc Hosp & Inst, Minist Educ, Intens Care Unit, Key Lab Carcinogenesis & Translat Res, Beijing, Peoples R China.;[Yang, Min] Cent South Univ, Xiangya Hosp 2, Dept Rehabil, Changsha, Hunan, Peoples R China.
通讯机构：
[Shi, Xiaoliu] C;[Zhou, Xi] H;Cent South Univ, Xiangya Hosp 2, Dept Med Genet, Changsha, Hunan, Peoples R China.;Hunan Normal Univ, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Coll Life Sci, Changsha, Hunan, Peoples R China.
摘要：
Myotonia congenita and hypokalemic periodic paralysis type 2 are both rare genetic channelopathies caused by mutations in the CLCN1 gene encoding voltage-gated chloride channel CLC-1 and the SCN4A gene encoding voltage-gated sodium channel Nav1.4. The patients with concomitant mutations in both genes manifested different unique symptoms from mutations in these genes separately. Here, we describe a patient with myotonia and periodic paralysis in a consanguineous marriage pedigree. By using whole-exome sequencing, a novel F306S variant in the CLCN1 gene and a known R222W mutation in the SCN4A gene were identified in the pedigree. Patch clamp analysis revealed that the F306S mutant reduced the opening probability of CLC-1 and chloride conductance. Our study expanded the CLCN1 mutation database. We emphasized the value of whole-exome sequencing for differential diagnosis in atypical myotonic patients.
摘要：
Objectives Cytokine activation and low complement levels are common in depression patients. This study is aimed at investigating the clinical significance of changes in serum concentrations of melatonin (MT), interleukin-6 (IL-6), homocysteine (hcy), and complement C3 and C4 in depression patients and relationships of them with depression activity. Methods A total of 95 depression patients, including first-episode group (n= 43) and recurrent group (n= 52), and 45 age- and gender-matched healthy controls (HC) were recruited. Serum levels of MT, IL-6, hcy, C3, and C4 in all samples were measured by using enzyme-linked immunosorbent assay (ELISA), chemiluminescence method, enzyme circulation method, and immuno-scatter turbidimetric assay, respectively. Results The serum MT, IL-6, and hcy levels in the first-episode group (113.08 +/- 5.06 pg/ml, 2.06 +/- 0.12 ng/L, and 13.87 +/- 0.45 mu mol/L), and recurrent group (117.63 +/- 4.63 pg/ml, 2.20 +/- 0.12 ng/L, and 13.61 +/- 0.46 mu mol/L) were significantly higher than those in the control group (89.50 +/- 5.10 pg/ml, 1.57 +/- 0.06 ng/L, and 11.34 +/- 0.40 mu mol/L). The serum levels of C3 in the first-episode group (0.95 +/- 0.02 ng/L) were significantly lower than those in the recurrent group (1.05 +/- 0.03 ng/L) and control group (1.12 +/- 0.03 ng/L). There was no significant difference in serum C4 level between each group. Conclusion These results suggest that higher serum MT, IL-6, and hcy levels were correlated with pathogenesis of depression.
作者机构：
[Huai Tao; Min Lu; Yuanyuan Wu; Dan Li; Yucheng Xiao] Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education,College of Life Sciences,Hunan Normal University,Changsha,Hunan 410081,China;[Huai Tao] Department of Biochemistry and Molecular Biology,Hunan University of Chinese Medicine,Changsha,Hunan,410208,China
会议名称：
10th IST Asia Pacific Congress on Animal,Plant and Microbial Toxins
会议时间：
2014-01-01
会议地点：
Changsha,China
会议主办单位：
中国毒理学会
会议论文集名称：
10th IST Asia Pacific Congress on Animal , Plant and Microbial Toxins(第十届亚太国际动物植物微生物毒素学术大会)论文集
摘要：
Spider venom is an important source of peptide molecules with different pharmacological properties.Jingzhaotoxin-1(JZTX-I) is a 33-residue peptide from the tarantula Chilobrachys jingzhao venom.Our pr
