Elevated Serum Matrix Metalloproteinase-9, Interleukin-6, Hypersensitive C-Reactive Protein, and Homocysteine Levels in Patients with Epilepsy
作者：
Tao, Huai;Gong, Yuji;Yu, Qi;Zhou, Hongfei;Liu, Yong
期刊：
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH ,2020年40(3):152-158 ISSN：1079-9907
通讯作者：
Liu, Y
作者机构：
[Zhou, Hongfei; Yu, Qi; Tao, Huai] Hunan Univ Chinese Med, Sch Med, Changsha, Peoples R China.;[Gong, Yuji] Huazhong Univ Sci & Technol, Dept Lab Med, Union Hosp, Tongji Med Coll, Wuhan, Peoples R China.;[Liu, Yong] Cent South Univ, Dept Psychiat, Xiangya Hosp 2, 139 Renmin Middle Rd, Changsha 410011, Hunan, Peoples R China.;[Liu, Yong] China Natl Clin Res Ctr Mental Disorders Xiangya, Changsha, Peoples R China.;[Liu, Yong] China Natl Technol Inst Mental Disorders, Changsha, Peoples R China.
通讯机构：
[Liu, Y ] C;Cent South Univ, Dept Psychiat, Xiangya Hosp 2, 139 Renmin Middle Rd, Changsha 410011, Hunan, Peoples R China.
关键词：
C reactive protein;gelatinase B;homocysteine;interleukin 6;latex;C reactive protein;gelatinase B;homocysteine;interleukin 6;adult;Article;chemoluminescence;clinical assessment;concentration process;controlled study;disease association;enzyme activation;enzyme linked immunosorbent assay;epilepsy;epileptic patient;female;generalized epilepsy;human;immunoturbidimetry;major clinical study;male;outcome assessment;priority journal;protein blood level;symptomatic epilepsy;adolescent;aged;analysis of variance;blood;case control study;epilepsy;middle aged;young adult;Adolescent;Adult;Aged;Analysis of Variance;C-Reactive Protein;Case-Control Studies;Epilepsy;Female;Homocysteine;Humans;Interleukin-6;Male;Matrix Metalloproteinase 9;Middle Aged;Young Adult
摘要：
Neuroinflammation contributes to the occurrence and development of epilepsy. However, several inflammatory factors that are important for facilitating the diagnosis to reduce or prevent seizures need to be further studied. This study is aimed to explore serum levels of matrix metalloproteinase-9 (MMP-9), interleukin-6 (IL-6), hypersensitive C-reactive protein (hs-CRP), and homocysteine (HCY) in epilepsy patients and the relationship of them with epilepsy. Epilepsy patients (n = 101) in the Second Xiangya Hospital from January 2017 to August 2018 were allocated to the epilepsy groups, which were divided into idiopathic epilepsy group (n = 43) and symptomatic epilepsy group (n = 58) according to the pathogeny. Healthy individuals (n = 50) were allocated to the control group. The concentrations of serum MMP-9, IL-6, hs-CRP, and HCY in all samples were detected by enzyme-linked immunosorbent assay, chemiluminescence method, latex-enhanced immunoturbidimetry, and enzyme circulation method. The levels of serum MMP-9, IL-6, hs-CRP, and HCY in epilepsy patients were higher than those in the control group (P < 0.05, P < 0.01, P < 0.01, and P < 0.01, respectively). The levels of serum MMP-9, IL-6, hs-CRP, and HCY in the symptomatic epilepsy group were higher than those in the control group (P < 0.01 or P < 0.05, respectively). The levels of serum MMP-9, IL-6, and hs-CRP in idiopathic epilepsy patients were higher than those in the control group (P < 0.01 or P < 0.05, respectively). The serum HCY level in the idiopathic epilepsy group was lower than that in the symptomatic epilepsy group (P < 0.01). MMP-9, IL-6, hs-CRP, and HCY may be recommended as the state biomarker to distinguish etiology of epilepsy. We hope our study could provide help in some ways for clinical diagnosis and treatment. © Mary Ann Liebert, Inc., publishers 2020.
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英文
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Jingzhaotoxin-X, a gating modifier of Kv4.2 and Kv4.3 potassium channels purified from the venom of the Chinese tarantula Chilobrachys jingzhao
作者：
Deng, Meichun;Jiang, Liping;Luo, Xuan;Tao, Huai;Liang, Songping
期刊：
JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES ,2020年26 ISSN：1678-9180
通讯作者：
Deng, MC
作者机构：
[Deng, Meichun; Deng, MC] Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha 410013, Hunan, Peoples R China.;[Jiang, Liping] Cent South Univ, Xiangya Sch Med, Dept Parasitol, Changsha 410013, Peoples R China.;[Luo, Xuan; Liang, Songping] Hunan Normal Univ, Coll Life Sci, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Changsha 410081, Peoples R China.;[Tao, Huai] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Deng, MC ] C;Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha 410013, Hunan, Peoples R China.
关键词：
jingzhaotoxin X;potassium channel blocking agent;potassium channel Kv4.2;potassium channel Kv4.3;sodium channel Nav1.5;sodium channel Nav1.7;spider venom;unclassified drug;voltage gated calcium channel;voltage gated potassium channel;amino acid sequence;animal experiment;animal model;Article;cation exchange;channel gating;Chilobrachys jingzhao;controlled study;disulfide bond;drug determination;drug effect;drug mechanism;drug potency;drug purification;embryo;human;human cell;intraplantar drug administration;mass spectrometry;mechanical hyperalgesia;molecular dynamics;motor performance;mouse;nonhuman;potassium current;reversed phase high performance liquid chromatography;rotarod test;tarantula
摘要：
Background: The tarantula Chilobrachys jingzhao is one of the largest venomous spiders in China. In previous studies, we purified and characterized at least eight peptides from C. jingzhao venom. In this report, we describe the purification and characterization of Jingzhaotoxin-X (JZTX-X), which selectively blocks Kv4.2 and Kv4.3 potassium channels. Methods: JZTX-X was purified using a combination of cation-exchange HPLC and reverse-phase HPLC. The amino-acid sequence was determined by automated Edman degradation and confirmed by mass spectrometry (MS). Voltage-gated ion channel currents were recorded in HEK293t cells transiently transfected with a variety of ion channel constructs. In addition, the hyperalgesic activity of JZTX-X and the toxin´s effect on motor function were assessed in mice. Results: JZTX-X contained 31 amino acids, with six cysteine residues that formed three disulfide bonds within an inhibitory cysteine knot (ICK) topology. In whole-cell voltage-clamp experiments, JZTX-X inhibited Kv4.2 and Kv4.3 potassium channels in a concentration- and voltage-dependent manner, without affecting other ion channels (Kv1.1, 1.2, 1.3, 2.1, delayed rectifier potassium channels, high- and low-voltage-activated Ca2+ channels, and voltage-gated sodium channels Nav1.5 and 1.7). JZTX-X also shifted the voltage-dependent channel activation to more depolarized potentials, whereas extreme depolarization caused reversible toxin binding to Kv4.2 channels. JZTX-X shifted the Kv4.2 and Kv4.3 activities towards a resting state, since at the resting potential the toxin completely inhibited the channels, even in the absence of an applied physical stimulus. Intrathecal or intraplantar injection of JZTX-X caused a long-lasting decrease in the mechanical nociceptive threshold (hyperalgesia) but had no effect on motor function as assessed in the rotarod test. Conclusions: JZTX-X selectively suppresses Kv4.2 and Kv4.3 potassium channel activity in a concentration- and voltage-dependent manner and causes long-lasting mechanical hyperalgesia. © The Author(s). 2020 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons-org-s.libyc.nudt.edu.cn:443/licenses/by/4.0/),
语种：
英文
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Altered Expression of Glucocorticoid Receptor and Neuron-Specific Enolase mRNA in Peripheral Blood in First-Episode Schizophrenia and Chronic Schizophrenia
作者：
Liu, Yong;Tang, Yamei;Li, Cunyan;Tao, Huai;Yang, Xiudeng;...
期刊：
FRONTIERS IN PSYCHIATRY ,2020年11:464940 ISSN：1664-0640
通讯作者：
Wang, XY
作者机构：
[Zhang, Xianghui; Wang, XY; Liu, Yong; Wang, Xuyi] Cent South Univ, Xiangya Hosp 2, Natl Clin Res Ctr Mental Disorders, Changsha, Peoples R China.;[Zhang, Xianghui; Wang, XY; Liu, Yong; Wang, Xuyi] Cent South Univ, Xiangya Hosp 2, Dept Psychaitry, Changsha, Peoples R China.;[Tang, Yamei] Cent South Univ, Xiangya Hosp 2, Dept Lab Med, Changsha, Peoples R China.;[Li, Cunyan] Hunan Normal Univ, Affiliated Hosp 1, Hunan Prov Peoples Hosp, Dept Lab Med, Changsha, Peoples R China.;[Tao, Huai] Hunan Univ Chinese Med, Dept Biochem & Mol Biol, Changsha, Peoples R China.
通讯机构：
[Wang, XY ] C;Cent South Univ, Xiangya Hosp 2, Natl Clin Res Ctr Mental Disorders, Changsha, Peoples R China.;Cent South Univ, Xiangya Hosp 2, Dept Psychaitry, Changsha, Peoples R China.
关键词：
chronic schizophrenia;cortisol;first-episode unmedicated schizophrenia (FES);glucocorticoid receptor;GR transcripts containing exons 1B (GR-1B);mRNA;neuron-specific enolase
摘要：
Introduction: It is well-known that altered hypothalamus–pituitary–adrenal (HPA) axis process has an important role in the neurodegenerative process in schizophrenia (SZ). However, this neurodegenerative mechanism has not been clarified in SZ. Therefore, the main purpose of this study was to determine HPA axis damage in the first-episode, unmedicated schizophrenia (FES) patients and chronic schizophrenia (CSZ) patients in comparison with healthy controls (HC) by means of quantitative analysis of the peripheral blood mRNA expression of glucocorticoid receptor (GR), GR transcripts containing exons 1B (GR-1B), and neuron specific enolase (NSE) genes and serum cortisol and NSE, a specific serum marker for neuronal damage. Methods: In the present study, 43 FES patients, 39 CSZ, and 47 HC were included. The peripheral blood mRNA expressions for GR, GR-1B, and NSE genes were determined by real-time quantitative polymerase chain reaction (RT-qPCR). Serum cortisol and NSE were analyzed by electrochemiluminescence immunoassay technique. Results: Levels of GR mRNA were significantly lower in FES and CSZ than that in HC. The expression of GR-1B mRNA was significantly decreased in CSZ when compared with that in FES. Levels of NSE mRNA were significantly lower in CSZ than that in FES patients or HC patients. CSZ patients showed significantly lower cortisol concentrations than FES and HC patients. FES patients showed significantly higher NSE concentrations than CSZ and HC. Conclusion: Our findings support that there is disrupted HPA axis system in the SZ and suggest that CSZ patients suffer a greater HPA axis damage than FES patients. Our research implicated underlying GR mRNA dysregulation in SZ and the potential importance of the functional GR-1B transcription in CSZ. © Copyright © 2020 Liu, Tang, Li, Tao, Yang, Zhang and Wang.
语种：
英文
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The Functional Role of Voltage-Gated Sodium Channel Nav1.5 in Metastatic Breast Cancer
作者：
Luo, Qianxuan;Wu, Ting;Wu, Wenfang;Chen, Gong;Luo, Xuan;...
期刊：
Frontiers in Pharmacology ,2020年11:527711 ISSN：1663-9812
通讯作者：
Deng, MC
作者机构：
[Chen, Gong; Wu, Wenfang; Deng, Meichun; Deng, MC; Luo, Qianxuan; Wu, Ting; Kang, Shuntong] Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha, Peoples R China.;[Chen, Gong; Wu, Wenfang; Deng, Meichun; Deng, MC; Luo, Qianxuan; Wu, Ting; Kang, Shuntong] Cent South Univ, Sch Life Sci, Hunan Prov Key Lab Basic & Appl Hematol, Changsha, Peoples R China.;[Deng, Meichun; Deng, MC; Luo, Qianxuan] Cent South Univ, Sch Life Sci, Hunan Key Lab Anim Models Human Dis, Changsha, Peoples R China.;[Deng, Meichun; Deng, MC; Luo, Qianxuan] Cent South Univ, Sch Life Sci, Hunan Key Lab Med Genet, Changsha, Peoples R China.;[Jiang, Liping; Wu, Ting; Kang, Shuntong] Cent South Univ, Xiangya Sch Med, Changsha, Peoples R China.
通讯机构：
[Deng, MC ] C;Cent South Univ, Sch Life Sci, Dept Biochem & Mol Biol, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Prov Key Lab Basic & Appl Hematol, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Key Lab Anim Models Human Dis, Changsha, Peoples R China.;Cent South Univ, Sch Life Sci, Hunan Key Lab Med Genet, Changsha, Peoples R China.
关键词：
Voltage-gated sodium channel (VGSC) Nav1.5;breast cancer;metastasis;Mechanism;inhibitors
摘要：
Voltage-gated sodium channels (VGSCs), which are abnormally expressed in various types of cancers such as breast cancer, prostate cancer, lung cancer, and cervical cancer, are involved in the metastatic process of invasion and migration. Nav1.5 is a pore-forming α subunit of VGSC encoded by SCN5A. Various studies have demonstrated that Nav1.5, often as its neonatal splice form, is highly expressed in metastatic breast cancer cells. Abnormal activation and expression of Nav1.5 trigger a variety of cellular mechanisms, including changing H+ efflux, promoting epithelial-to-mesenchymal transition (EMT) and the expression of cysteine cathepsin, to potentiate the metastasis and invasiveness of breast cancer cells in vitro and in vivo. Here, we systematically review the latest available data on the pro-metastatic effect of Nav1.5 and its underlying mechanisms in breast cancer. We summarize the factors affecting Nav1.5 expression in breast cancer cells, and discuss the potential of Nav1.5 blockers serving as candidates for breast cancer treatment. © Copyright © 2020 Luo, Wu, Wu, Chen, Luo, Jiang, Tao, Rong, Kang and Deng.
语种：
英文
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Myotonia congenita and periodic hypokalemia paralysis in a consanguineous marriage pedigree: Coexistence of a novel CLCN1 mutation and an SCN4A mutation
作者：
Zhao, Chenyu;Tang, DongFang;Huang, Hui;Tang, Haiyan;Yang, Yuan;...
期刊：
PLOS ONE ,2020年15(5):e0233017 ISSN：1932-6203
通讯作者：
Shi, XL;Zhou, X
作者机构：
[Yang, Yuan; Zhao, Chenyu; Shi, Xiaoliu; Huang, Hui; Tang, Haiyan] Cent South Univ, Xiangya Hosp 2, Dept Med Genet, Changsha, Hunan, Peoples R China.;[Zhao, Chenyu] Cent South Univ, Xiangya Hosp 2, Dept Gastroenterol, Changsha, Hunan, Peoples R China.;[Tang, DongFang; Zhou, Xi] Hunan Normal Univ, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Coll Life Sci, Changsha, Hunan, Peoples R China.;[Yang, Yuan] Peking Univ Canc Hosp & Inst, Minist Educ, Intens Care Unit, Key Lab Carcinogenesis & Translat Res, Beijing, Peoples R China.;[Yang, Min] Cent South Univ, Xiangya Hosp 2, Dept Rehabil, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhou, X ] H;[Shi, XL ] C;Cent South Univ, Xiangya Hosp 2, Dept Med Genet, Changsha, Hunan, Peoples R China.;Hunan Normal Univ, Natl & Local Joint Engn Lab Anim Peptide Drug Dev, Coll Life Sci, Changsha, Hunan, Peoples R China.
摘要：
Myotonia congenita and hypokalemic periodic paralysis type 2 are both rare genetic channelopathies caused by mutations in the CLCN1 gene encoding voltage-gated chloride channel CLC-1 and the SCN4A gene encoding voltage-gated sodium channel Nav1.4. The patients with concomitant mutations in both genes manifested different unique symptoms from mutations in these genes separately. Here, we describe a patient with myotonia and periodic paralysis in a consanguineous marriage pedigree. By using whole-exome sequencing, a novel F306S variant in the CLCN1 gene and a known R222W mutation in the SCN4A gene were identified in the pedigree. Patch clamp analysis revealed that the F306S mutant reduced the opening probability of CLC-1 and chloride conductance. Our study expanded the CLCN1 mutation database. We emphasized the value of whole-exome sequencing for differential diagnosis in atypical myotonic patients.
语种：
英文
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Changes of Serum Melatonin, Interleukin-6, Homocysteine, and Complement C3 and C4 Levels in Patients With Depression
作者：
Tao, Huai;Chen, Xia;Zhou, Hongfei;Fu, Jinhua;Yu, Qi;...
期刊：
FRONTIERS IN PSYCHOLOGY ,2020年11:543152 ISSN：1664-1078
通讯作者：
Liu, Y
作者机构：
[Zhou, Hongfei; Yu, Qi; Tao, Huai] Hunan Univ Chinese Med, Sch Med, Changsha, Peoples R China.;[Chen, Xia] Cent South Univ, Xiangya Hosp 2, Dept Orthoped, Changsha, Peoples R China.;[Fu, Jinhua] Hunan Prov Brain Hosp, Dept Psychiat, Changsha, Peoples R China.;[Liu, Yong] Cent South Univ, Xiangya Hosp 2, Dept Psychaitry,Hunan Key Lab Psychiat & Mental H, Natl Clin Res Ctr Mental Disorders,China Natl Tec, Changsha, Peoples R China.
通讯机构：
[Liu, Y ] C;Cent South Univ, Xiangya Hosp 2, Dept Psychaitry,Hunan Key Lab Psychiat & Mental H, Natl Clin Res Ctr Mental Disorders,China Natl Tec, Changsha, Peoples R China.
关键词：
complement;depression;homocysteine;interleukin-6;melatonin
摘要：
Objectives: Cytokine activation and low complement levels are common in depression patients. This study is aimed at investigating the clinical significance of changes in serum concentrations of melatonin (MT), interleukin-6 (IL-6), homocysteine (hcy), and complement C3 and C4 in depression patients and relationships of them with depression activity. Methods: A total of 95 depression patients, including first-episode group (n = 43) and recurrent group (n = 52), and 45 age- and gender-matched healthy controls (HC) were recruited. Serum levels of MT, IL-6, hcy, C3, and C4 in all samples were measured by using enzyme-linked immunosorbent assay (ELISA), chemiluminescence method, enzyme circulation method, and immuno-scatter turbidimetric assay, respectively. Results: The serum MT, IL-6, and hcy levels in the first-episode group (113.08 ± 5.06 pg/ml, 2.06 ± 0.12 ng/L, and 13.87 ± 0.45 μmol/L), and recurrent group (117.63 ± 4.63 pg/ml, 2.20 ± 0.12 ng/L, and 13.61 ± 0.46 μmol/L) were significantly higher than those in the control group (89.50 ± 5.10 pg/ml, 1.57 ± 0.06 ng/L, and 11.34 ± 0.40 μmol/L). The serum levels of C3 in the first-episode group (0.95 ± 0.02 ng/L) were significantly lower than those in the recurrent group (1.05 ± 0.03 ng/L) and control group (1.12 ± 0.03 ng/L). There was no significant difference in serum C4 level between each group. Conclusion: These results suggest that higher serum MT, IL-6, and hcy levels were correlated with pathogenesis of depression. © Copyright © 2020 Tao, Chen, Zhou, Fu, Yu and Liu.
语种：
英文
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电压门控钠通道亚型基因突变相关癫痫发生和临床治疗
作者：
陶怀;薛卢嘉黛;陈夏
期刊：
中国临床药理学与治疗学 ,2018年23(5):570-577 ISSN：1009-2501
作者机构：
湖南中医药大学医学院生物化学教研室, 湖南, 长沙, 410208;中南大学湘雅二医院骨科, 湖南, 长沙, 410011;[陶怀; 薛卢嘉黛] 湖南中医药大学医学院生物化学教研室, 湖南, 长沙, 410208;[陈夏] 中南大学湘雅二医院骨科, 湖南, 长沙, 410011
关键词：
电压门控钠通道;基因突变;癫痫;临床治疗
摘要：
癫痫（epilepsy,EP）是一种由大脑神经元异常放电所引起的以短暂的大脑功能失常为特征的常见脑部疾病,易反复发作,严重影响患者的健康和生活质量。研究发现,表达在中枢神经系统中电压门控钠通道（voltage-gated sodium channel,VGSC）亚型Nav1.1、Nav1.2、Nav1.3、Nav1.6、Nav1.7在癫痫发病机制中起重要作用,是相关抗癫痫药物（AEDs）的作用靶点。这些通道α亚基编码基因突变可引起不同形式的癫痫发作。本文针对表达在中枢神经系统中的上述5种VGSC亚型基因突变相关癫痫发生和临床治疗作简要综述,为癫痫的发生发展电生理机制和临床治疗研究奠定基础。
语种：
中文
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卵巢早衰验案举隅
作者：
胡齐欣;杨正望;熊芬
期刊：
亚太传统医药 ,2018年14(2):134-135 ISSN：1673-2197
作者机构：
[熊芬; 胡齐欣] 湖南中医药大学;湖南中医药大学第一附属医院;[杨正望] 中医药大学第一附属医院
关键词：
卵巢早衰;补;通;养;杨正望
摘要：
卵巢早衰（premature ovarian insufficiency,POI）是指40岁之前丧失卵巢活性,表现为闭经、不孕,伴有促性腺素升高和雌激素降低,是困扰育龄期妇女的内分泌疾病之一。导师杨正望教授认为卵巢早衰的基本病机以肾虚为本,累及心、肝、脾,临床擅于运用"补、通、养"三字诀,辨证治疗卵巢早衰,效果显著。
语种：
中文
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基于文献汇报培养大学生创新性科研思维
作者：
陶怀;何迎春;陈夏
期刊：
当代教育实践与教学研究（电子版） ,2017年(7):134-134
作者机构：
湖南中医药大学医学院生物化学与分子生物学教研室;中南大学湘雅二医院
关键词：
文献汇报;大学生;创新性思维
摘要：
在大学生创新性课题指导中，教师指导学生阅读文献，并要求学生针对精读、翻译的文献进行汇报并讨论交流，以促进学生对前沿科研进展的关注和科研问题的把握，从中掌握科研技术和方法，培养学生创新性科研思维。
语种：
中文
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大数据时代医学分子生物学教学改革的几点思考
作者：
陶怀;张波;周芳亮;宋岚;何迎春
期刊：
卫生职业教育 ,2017年35(8):34-35 ISSN：1671-1246
作者机构：
湖南中医药大学,湖南 长沙,410208
关键词：
大数据时代;医学分子生物学;教学改革
摘要：
大数据的新形势下,必须要把握大数据时代的特征,充分利用网络资源,跟踪国内外前沿知识,从教学内容、教学方法、教学理念、教师队伍优化方面对医学分子生物学进行教学改革.
语种：
中文
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基于Web Quest模式的分子生物学开放性实验改革初探
期刊：
山西医科大学学报 ,2016年18(4):315-318 ISSN：1007-6611
作者机构：
湖南中医药大学生物化学与分子生物学教研室,长沙,410208;[陈夏] 中南大学湘雅二医院;[何迎春; 张波; 宋岚; 陶怀] 湖南中医药大学
关键词：
分子生物学;开放性实验;创新能力
摘要：
为了培养中医专业七年制学生的创新科研思维能力、实际动手能力和分析问题能力,旨在Web Quest模式的基础上,以本科生导师制方式探索开设分子生物学开放性实验,提高学生的综合素质,为培养具备创新性科研能力的中医药人才奠定坚实的基础。
语种：
中文
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Overexpression of AKR1B10 in nasopharyngeal carcinoma as a potential biomarker
作者：
He, Ying-Chun;Shen, Yi;Cao, Yu;Tang, Fang-Qing;Tian, Dao-Fa;...
期刊：
CANCER BIOMARKERS ,2016年16(1):127-135 ISSN：1574-0153
通讯作者：
Cao, DL;Liao, DF
作者机构：
[Zhou, Fang-Liang; He, Ying-Chun; Liao, Duan-Fang; Tian, Dao-Fa; Song, Lan; Lu, Fang-Guo; Lin, Li-Mei; Zhang, Bo; Cao, Deliang; He, Lan; Tao, Huai] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Div Stem Cell Regulat & Applicat, Changsha, Hunan, Peoples R China.;[Cao, Deliang; Shen, Yi; He, Ying-Chun; Cao, Yu; Huang, Chen-Fei] So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.;[Tang, Fang-Qing] First Peoples Hosp Zhuhai, Zhuhai, Guangdong, Peoples R China.;[Liao, DF] Hunan Univ Tradit Chinese Med, Div Stem Cell Regulat & Applicat, 1 Xiangzui Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Liao, DF ] H;[Cao, DL ] S;So Illinois Univ, Sch Med, Simmons Canc Inst, Dept Med Microbiol Immunol & Cell Biol, 913 N Rutledge St, Springfield, IL 62794 USA.;Hunan Univ Tradit Chinese Med, Div Stem Cell Regulat & Applicat, 1 Xiangzui Rd, Changsha 410208, Hunan, Peoples R China.
会议名称：
2015全国中医药生物化学与分子生物学年会
会议时间：
2015-08-01
会议地点：
兰州
会议论文集名称：
2015全国中医药生物化学与分子生物学年会论文集
关键词：
Aldo-keto reductase 1B10;nasopharyngeal carcinoma;tumor marker,squamous cell carcinoma;nasopharyngeal lesions
摘要：
BACKGROUND: Nasopharyngeal carcinoma (NPC) is one of the most common cancers in Southern China. Aldo-keto reductase 1B10 (AKR1B10) is upregulated in multiple tumors and plays an oncogenic role. OBJECTIVE: To examine the expression of AKR1B10 at mRNA and protein levels in nasopharyngeal tumors and correlate its expression with clinicopathological parameters. METHODS: A tissue microarray, paraffin blocks, and frozen surgical nasopharyngeal samples were procured. Western blot and immunohistochemistry were used to estimate AKR1B10 protein expression, and mRNA levels were detected by real time RT-PCR. RESULTS: We found that AKR1B10 expression was increased in malignant tissues compared to the normal tissues (p= 0.000). In NPC tissues, AKR1B10 expression appeared high specifically in squamous cell carcinoma, but low in basal cell carcinoma, adenoid cystic carcinoma, adenocarcinoma and undifferentiated carcinoma (p= 0.000). AKR1B10 expression also demonstrated correlation with tumor differentiation, with a high level in well and moderately differentiated but a low level in poorly differentiated carcinoma (p= 0.000). AKR1B10 was also upregulated in hyperplasia and benign tumors (p= 0.000), and demonstrated a specific nuclear distribution in these non-cancerous diseases. CONCLUSIONS: AKR1B10 is overexpressed in nasopharyngeal hyperplasia, benign tumors, and carcinomas, being a potential new biomarker. © 2016 - IOS Press and the authors. All rights reserved.
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英文
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The molecular interaction of tarantula toxin jingzhaotoxin-I with voltage-gated sodium channel subtype Nav1.5
作者：
Huai Tao;Min Lu;Yuanyuan Wu;Dan Li;Yucheng Xiao
作者机构：
[Huai Tao; Min Lu; Yuanyuan Wu; Dan Li; Yucheng Xiao] Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education,College of Life Sciences,Hunan Normal University,Changsha,Hunan 410081,China;[Huai Tao] Department of Biochemistry and Molecular Biology,Hunan University of Chinese Medicine,Changsha,Hunan,410208,China
会议名称：
10th IST Asia Pacific Congress on Animal,Plant and Microbial Toxins
会议时间：
2014-06-14
会议地点：
Changsha,China
关键词：
spider toxin;mutation;voltage-gated sodium channel
摘要：
Spider venom is an important source of peptide molecules with different pharmacological properties.Jingzhaotoxin-1(JZTX-I) is a 33-residue peptide from the tarantula Chilobrachys jingzhao venom.Our pr
语种：
英文
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