作者机构：
[Song, Hou-Pan; Cai, Xiong; Huang, Hui-Yong] Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha 410208, Hunan, Peoples R China.;[Song, Hou-Pan; Li, Ru-Liu] Guangzhou Univ Chinese Med, Spleen & Stomach Inst, Guangzhou 510405, Guangdong, Peoples R China.;[Zhou, Chi] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Guangdong, Peoples R China.;[Song, Hou-Pan] Hunan Univ Chinese Med, Inst TCM Diagnost, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Song, Hou-Pan] H;Hunan Univ Chinese Med, Inst TCM Diagnost, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
Atractylodes macrocephala Koidz;Intestinal epithelial cells;Cell migration;Polyamines;Rho GTPases;Non-muscle myosin II
摘要：
Ethnopharmacological relevance: Atractylodes macrocephala Koidz (AMK), a valuable traditional Chinese herbal medicine, has been widely used in clinical practice for treating patients with disorders of the digestive system. AMK has shown noteworthy promoting effect on improving gastrointestinal function and immunity, which might represent a promising candidate for the treatment of intestinal mucosa injury. The aim of this study was to investigate the efficacy of AMK on intestinal mucosal restitution and the underlying mechanisms via intestinal epithelial (IEC-6) cell migration model. Materials and methods: A cell migration model of IEC-6 cells was induced by a single-edge razor blade along the diameter of the cell layers in six-well polystyrene plates. After wounding, the cells were grown in control cultures and in cultures containing spermidine (5 mu M, SPD, reference drug), alphadifluoromethylornithine (2.5 mM, DFMO, polyamine inhibitor), AMK (50, 100, and 200 mg/L), DFMO plus SPD and DEMO plus AMK for 12 h. The polyamines content was detected by high-performance liquid chromatography (HPLC) with pre-column derivatization. The Rho mRNAs expression levels were assessed by Q-RT-PCR. The Rho and non-muscle myosin II proteins expression levels were analyzed by Western blot. The formation and distribution of non-muscle myosin II stress fibers were monitored with immunostaining techniques using specific antibodies and observed by confocal microscopy. Cell migration assay was carried out using inverted microscope and the Image-Pro Plus software. All of these indexes were used to evaluate the effectiveness of AMK. Results: (1) Treatment with AMK caused significant increases in cellular polyamines content and Rho mRNAs and proteins expression levels, as compared to control group. Furthermore, AMK exposure increased non-muscle myosin II protein expression levels and formation of non-muscle myosin II stress fibers, and resulted in an acceleration of cell migration in IEC-6 cells. (2) Depletion of cellular polyamines by DEMO resulted in a decrease of cellular polyamines levels. Rho mRNAs and proteins expression, nonmuscle myosin II protein formation and distribution, thereby inhibiting IEC-6 cell migration. AMK not only reversed the inhibitory effects of DFMO on the polyamines content, Rho mRNAs and proteins expression, non-muscle myosin II protein formation and distribution, but also restored cell migration to control levels. Conclusions: The results obtained from this study revealed that AMK significantly stimulates the migration of IEC-6 cells through a polyamine dependent mechanism, which could accelerate the healing of intestinal injury. These findings suggest the potential value of AMK in curing intestinal diseases characterized by injury and ineffective repair of the intestinal mucosa in clinical practice. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
期刊：
Journal of Acupuncture and Meridian Studies,2015年8(2):61-65 ISSN：2005-2901
通讯作者：
Huang, HY
作者机构：
[He, Junfeng; Zhang, Xing] Affiliated Hosp Hunan Univ Chinese Med, Liuyang Hosp Tradit Chinese Med, Liuyang City, Peoples R China.;[Du, Jia; Huang, Huiyong; Qu, Yating; Liu, Xia; Guo, Shengtong] Hunan Univ Chinese Med, Inst TCM Diagnost, 1 Xiangzui Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Huang, HY ] ;Institute of TCM Diagnostic, Hunan University of Chinese Medicine, Hanpu Science and Education District, Number 1 Xiangzui Road, Changsha, Hunan Province, China
关键词：
acupuncture;body mass index;massage;obesity;overweight
摘要：
Obesity is one of the leading health risk factors worldwide and is associated with several other risk factors and health problems. Acupuncture is utilized to treat a variety of health problems, one of which is obesity. Fifty-six obese women with body mass index (BMI) >/=25 kg/m(2) were recruited for this trial and were randomly divided into two groups, one with combined manual acupuncture and massage therapy (MAMT), and the other with only manual acupuncture therapy (MAT). In addition, 40 overweight women with BMI 23-25 kg/m(2) were randomly divided into two groups, one with MAMT and the other with MAT. Therapy was carried out once per day for 21 days, and the body weights and the BMIs were recorded every day. The results showed that both MAMT and MAT could reduce body weight and BMI significantly, compared with the pretreatment values, for all the participants (p < 0.001); however, the differences in body weight and BMI reductions between pre- and posttreatment for the MAMT and the MAT groups were not statistically significant. The optimal periods for reductions in both body weight and in BMI were the first 4 days. Accounting for the economic strategy (time and money) in alternative therapy, MAT alone may present a reasonable option in the treatment of overweight and obesity in adults.
