关键词：
Chaihu-Longgu-Muli decoction;Hippocampal neurons;Pyroptosis;Temporal lobe epilepsy;Traditional Chinese medicine
摘要：
Ethnopharmacological relevance: Chaihu-Longgu-Muli Decoction (CLMD) is a classic prescription created by Zhong-jing Zhang, a famous ancient Chinese medical scientist, to harmonize uncontrollable body activities and calm the minds. Now Traditional Chinese Medicine (TCM) physicians often apply it to treat psychiatric diseases such as epilepsy. Aim of the study: This study investigated the mechanism of the effect of Chaihu-Longgu-Muli Decoction (CLMD) on hippocampal neurons pyroptosis in rats with Temporal Lobe Epilepsy (TLE). Materials and methods: The lithium chloride-pilocarpine-induced TLE rat model was established. The behavioral testing was performed and, the expression of IL-1 beta and TNF-alpha in serum was detected by ELISA, qRT-PCR was used to detect the mRNA expression of NLRP3, Caspase-1, IL-1 beta and TNF-alpha in hippocampus. The expression of NLRP3 and Caspase-1 in hippocampal dentate gyrus was detected by immunofluorescence assay. Results: CLMD could significantly suppress the frequency and duration time of epileptic seizures, reduce the expression of NLRP3, Caspase-1 TNF-alpha and IL-1 beta. Conclusions: CLMD exerted an obvious antiepileptic effect by improving pyroptosis in hippocampal neurons of TLE rats.
期刊：
BioMed Research International,2020年2020 ISSN：2314-6133
通讯作者：
Li, Liang;Huang, Huiyong;Li, Weiqing
作者机构：
[Yuan, Zhiying; Wu, Huaying; Li, Liang; Huang, Huiyong; Zhang, Shiying] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Luohu Peoples Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Univ, Dept Tradit Chinese Med, Affiliated Hosp 2, Shenzhen, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Luohu Peoples Hosp, Shenzhen Luohu Hosp Grp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.
通讯机构：
[Li, L; Huang, HY] H;[Li, Weiqing] S;Hunan Univ Chinese Med, Changsha, Peoples R China.;Shenzhen Luohu Peoples Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;Shenzhen Univ, Dept Tradit Chinese Med, Affiliated Hosp 2, Shenzhen, Peoples R China.
摘要：
Objective. To explore the effects of the Hedysarum multijugum Maxim.-Radix Salviae compound (Huangqi-Danshen Compound (HDC)) on oxidative capacity and cardiomyocyte apoptosis in rats with diabetic cardiomyopathy by a network pharmacology-based strategy. Methods. Traditional Chinese Medicine (TCM)@Taiwan, TCM Systems Pharmacology Database and Analysis Platform (TCMSP), TCM Integrated Database (TCMID), and High-Performance Liquid Chromatography (HPLC) technology were used to obtain and screen HDC's active components, and the PharmMapper database was used to predict HDC human target protein targets. The DCM genes were collected from the GeneCards and OMIM databases, and the network was constructed and analyzed by Cytoscape 3.7.1 and the Database for Annotation, Visualization, and Integrated Discovery (DAVID). Finally, HDC was used to intervene in diabetic cardiomyopathy (DCM) model rats, and important biological processes and signaling pathways were verified using techniques such as immunohistochemistry. Results. A total of 176 of HDC's active components and 442 potential targets were obtained. The results of network analysis show that HDC can regulate DCM-related biological processes (such as negative regulation of the apoptotic process, response to hypoxia, the steroid hormone-mediated signaling pathway, cellular iron ion homeostasis, and positive regulation of phosphatidylinositol 3-kinase signaling) and signaling pathways (such as the HIF-1 signaling pathway, the estrogen signaling pathway, insulin resistance, the PPAR signaling pathway, the VEGF signaling pathway, and the PI3K-Akt signaling pathway). Animal experiments show that HDC can reduce fasting plasma glucose (FPG), HbA1c, and malondialdehyde (MDA) and increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P0.05). The results of immunohistochemistry showed that HDC can regulate the protein expression of apoptosis-related signaling pathways in DCM rats (P0.05). Conclusion. It was initially revealed that HDC improves DCM through its antiapoptotic and anti-inflammatory effects. HDC may play a therapeutic role by improving cardiomyocyte apoptosis in DCM rats.
作者机构：
[Yi-Zhi M.; Hui-Yong H.; Zhi-Ying Y.; Hua-Ying W.; Jing L.] Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Feng L.] Dental / Head and Neck Cancer Research Center, UCLA School of Dentistry, Los Angeles, CA 90095, United States;[Liang L.] Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China, Dental / Head and Neck Cancer Research Center, UCLA School of Dentistry, Los Angeles, CA 90095, United States
通讯机构：
[Huang Hui-Yong] P;Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
关键词：
Coronavirus disease 2019 (COVID-19);Hunan expert group recommended Chinese medicine prevention No. 2 prescription (Pre-No. 2);Immune system;Network pharmacology
