期刊：
BioMed Research International,2020年2020:1-17 ISSN：2314-6133
通讯作者：
Li, Liang;Huang, HY;Li, WQ
作者机构：
[Yuan, Zhiying; Wu, Huaying; Huang, Huiyong; Li, Liang; Zhang, Shiying] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Luohu Peoples Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Univ, Dept Tradit Chinese Med, Affiliated Hosp 2, Shenzhen, Peoples R China.;[Zhang, Shiying; Li, Weiqing] Shenzhen Luohu Peoples Hosp, Shenzhen Luohu Hosp Grp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.
通讯机构：
[Huang, HY ; Li, L] H;[Li, WQ ] S;Hunan Univ Chinese Med, Changsha, Peoples R China.;Shenzhen Luohu Peoples Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;Shenzhen Univ, Dept Tradit Chinese Med, Affiliated Hosp 2, Shenzhen, Peoples R China.
摘要：
<jats:p>Objective. To explore the effects of the Hedysarum multijugum Maxim.-Radix Salviae compound (Huangqi-Danshen Compound (HDC)) on oxidative capacity and cardiomyocyte apoptosis in rats with diabetic cardiomyopathy by a network pharmacology-based strategy. Methods. Traditional Chinese Medicine (TCM)@Taiwan, TCM Systems Pharmacology Database and Analysis Platform (TCMSP), TCM Integrated Database (TCMID), and High-Performance Liquid Chromatography (HPLC) technology were used to obtain and screen HDC’s active components, and the PharmMapper database was used to predict HDC human target protein targets. The DCM genes were collected from the GeneCards and OMIM databases, and the network was constructed and analyzed by Cytoscape 3.7.1 and the Database for Annotation, Visualization, and Integrated Discovery (DAVID). Finally, HDC was used to intervene in diabetic cardiomyopathy (DCM) model rats, and important biological processes and signaling pathways were verified using techniques such as immunohistochemistry. Results. A total of 176 of HDC’s active components and 442 potential targets were obtained. The results of network analysis show that HDC can regulate DCM-related biological processes (such as negative regulation of the apoptotic process, response to hypoxia, the steroid hormone-mediated signaling pathway, cellular iron ion homeostasis, and positive regulation of phosphatidylinositol 3-kinase signaling) and signaling pathways (such as the HIF-1 signaling pathway, the estrogen signaling pathway, insulin resistance, the PPAR signaling pathway, the VEGF signaling pathway, and the PI3K-Akt signaling pathway). Animal experiments show that HDC can reduce fasting plasma glucose (FPG), HbA1c, and malondialdehyde (MDA) and increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (<jats:inline-formula>
<math xmlns="http://www.w3.org/1998/Math/MathML" id="M1">
<mi>P</mi>
<mo><</mo>
<mn>0.05</mn>
</math>
</jats:inline-formula>). The results of immunohistochemistry showed that HDC can regulate the protein expression of apoptosis-related signaling pathways in DCM rats (<jats:inline-formula>
<math xmlns="http://www.w3.org/1998/Math/MathML" id="M2">
<mi>P</mi>
<mo><</mo>
<mn>0.05</mn>
</math>
</jats:inline-formula>). Conclusion. It was initially revealed that HDC improves DCM through its antiapoptotic and anti-inflammatory effects. HDC may play a therapeutic role by improving cardiomyocyte apoptosis in DCM rats.</jats:p>
期刊：
BioMed Research International,2020年2020:1-12 ISSN：2314-6133
通讯作者：
Qiu, XJ;Huang, HY
作者机构：
[Qiu, Xinjian; Xu, Xia; Yan, Lijing; Wang, Dongsheng; Qiu, XJ] Cent South Univ, Xiangya Hosp, Inst Integrated Tradit Chinese & Western Med, Lab Ethnopharmacol, Changsha 410008, Hunan, Peoples R China.;[He, Zhenyu] Taizhou Hosp Integrated Tradit Chinese & Western, Taizhou 317500, Zhejiang, Peoples R China.;[Wang, Sheng] Zhejiang Chinese Med Univ, Clin Med Coll 2, Hangzhou 310053, Zhejiang, Peoples R China.;[Zhao, Linlin] Cent South Univ, Dept Hlth Management, Xiangya Hosp 3, Changsha 410013, Hunan, Peoples R China.;[Qiu, Juan] Cent South Univ, Dept Nucl Med, Xiangya Hosp 4, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Huang, HY ] H;[Qiu, XJ ] C;Cent South Univ, Xiangya Hosp, Inst Integrated Tradit Chinese & Western Med, Lab Ethnopharmacol, Changsha 410008, Hunan, Peoples R China.;Hunan Univ Tradit Chinese Med, Inst Tradit Chinese Med Diag, Changsha 410208, Hunan, Peoples R China.
摘要：
<jats:p><jats:italic>Background</jats:italic>. Fluoxetine (FLU) is the first-line and widely used medication for depression; however, FLU treatment is almost ineffective in 30%-40% of patients with depression. In addition, there are some problems in FLU treatment, such as delayed efficacy, large side effects, and poor tolerance. Chaihu Shugan San (CSS) is a classic and effective antidepressant Chinese herbal medicine that has been used in China for thousands of years. CSS or coadministration of CSS and FLU has become one of the most recommended methods in the treatment of depression in China. However, the specific pathways of CSS and coadministration of CSS and FLU for antidepressant are still unclear. <jats:italic>Objective</jats:italic>. This study was designed to evaluate the antidepressant effects of CSS and coadministration of CSS and FLU. <jats:italic>Methods</jats:italic>. The chronic unpredictable mild stress (CUMS) rat model was used to simulate depression. 120 healthy adult male Sprague-Dawley (SD) rats were randomly divided into seven groups: the control group, CUMS group, low-dose CSS group, high-dose CSS group, FLU group, coadministration of low-dose CSS and FLU group, and coadministration of high-dose CSS and FLU group. The rats in different groups were given different interventions. Then, the depression-like behavior and cognitive function were evaluated by the sucrose preference test (SPT), forced swimming test (FST), open field test (OFT), and Y-maze test. What is more, the antidepressant mechanism of CSS and coadministration of CSS and FLU were studied through BDNF mRNA, ERK mRNA, CREB mRNA, BDNF, p-ERK/ERK, and p-CREB/CREB levels in the hippocampus and frontal cortex by Western blot and RT-PCR. <jats:italic>Results</jats:italic>. Compared with the CUMS group, CSS and coadministration of CSS and FLU could alleviate the depressive symptoms and improve cognitive function in CUMS rats (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:math>); CSS and coadministration of CSS and FLU could increase the expression of BDNF, p-CREB/CREB, p-ERK/ERK, and BDNF mRNA, CREB mRNA, and ERK mRNA in the hippocampus and frontal cortex (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:math>). Besides, the high-dose CSS combined with the fluoxetine group was significantly better than the fluoxetine group and CSS group (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mi>p</mml:mi><mml:mo><</mml:mo><mml:mn>0.05</mml:mn></mml:math>). <jats:italic>Discussion and Conclusion</jats:italic>. Finally, we found that both CSS and coadministration of CSS and FLU play an antidepressant role, which may be due to the regulation of the BDNF/ERK/CREB signaling pathway in the hippocampus and frontal cortex. Among them, the coadministration of CSS and FLU can enhance the antidepressant effect of CSS or FLU alone, and the underlying mechanism needs further investigation.</jats:p>
作者机构：
[Yi-Zhi M.; Hui-Yong H.; Zhi-Ying Y.; Hua-Ying W.; Jing L.] Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;[Feng L.] Dental / Head and Neck Cancer Research Center, UCLA School of Dentistry, Los Angeles, CA 90095, United States;[Liang L.] Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China, Dental / Head and Neck Cancer Research Center, UCLA School of Dentistry, Los Angeles, CA 90095, United States
通讯机构：
[Huang Hui-Yong] P;Provincial Key Laboratory of TCM Diagnostics, Hunan Engineering Technology Research Center for Medicinal and Functional Food, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
关键词：
Coronavirus disease 2019 (COVID-19);Hunan expert group recommended Chinese medicine prevention No. 2 prescription (Pre-No. 2);Immune system;Network pharmacology
