期刊：
Journal of Ethnopharmacology,2020年263:113156- ISSN：0378-8741
通讯作者：
Zeng, Liuting;Yang, Kailin;Liu, Huiping;Lei, Lei
作者机构：
[Zeng, Liuting] Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China.;[Zeng, Liuting] Minist Educ, Key Lab Rheumatol & Clin Immunol, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing Anzhen Hosp, Beijing, Peoples R China.;[Liu, Liting] Huazhong Univ Sci & Technol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Zeng, Liuting; Yang, Kailin] C;[Liu, HP; Lei, L] H;Chinese Acad Med Sci & Peking Union Med Coll, Peking Union Med Coll Hosp, Dept Rheumatol & Clin Immunol, Beijing, Peoples R China.;Capital Med Univ, Beijing, Peoples R China.;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Shoutai Wan (STW) is a classic herbal formula for the treatment of recurrent spontaneous abortion (RSA), and clinical studies have shown the effectiveness of STW on RSA. However, the molecular mechanism of STW treatment of RSA is still unclear. METHODS: (1) Animal experiments: The normal pregnancy model was established with CBA/J*BALB/C, and the RSA model was established by CBA/J*DBA/2. The RSA model CBA/J*DBA/2 pregnant mice were randomly divided into four groups (RSA model group, STW low, medium and high dose groups) according to the order of pregnancy, respectively. The drug administration starts from the first day of pregnancy to the 14th day of pregnancy. The embryo loss rate (ELR) of each group was calculated. (2) Proteomic analysis of decidual tissue: The total protein of decidual tissue of each group was isolated by solid phase pH gradient 2-DE technique. The differentially expressed protein spots were analyzed and identified by PDQuest images; the peptide quality fingerprinting (PMF) was obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Then, the proteins were identified by Mascot software searching, their functions were identified by bioinformatics strategy. (3) The expression of HSP27, α-enolase and Transferrin was detected by Western blotting and the expression of Annexin A2 and Transferrin was detected by immunohistochemistry. (4) The differential proteins and potential targets were analyzed by systematic biological strategy. RESULTS: (1) Compared with the normal group, the ELR in the RSA model group was significantly higher (P < 0.01). Compared with the model group, the ELR in the STW high, medium dose groups was lower (P < 0.01). (2) A 2-DE map of the decidual tissue of the RSA model group, normal pregnancy group, STW low, medium and high dose groups was established. Thirty proteins were identified. (3) The results of western blot showed that the expression of HSP27 and a-enolase in the RSA model group was higher than that in the normal group, and the expression of transferrin was lower (P <0.01). Compared with the model group, the expression of HSP27 and a-enolase in STW high, medium dose groups was decreased (P <0.01); Compared with the model group, the expression of Transferrin in the STW high dose group was increased (P < 0.01). (5) A lot of RSA treatment-related targets, biological processes and pathways were found after the systematic biological analysis. CONCLUSION: (1) STW may reduce the ELR of the RSA mice. (2) The results of proteomics suggest that RSA is a complex process involving multiple proteins. STW can regulate the expression of various proteins in the decidual tissue of RSA mice, suggesting that it can act on multiple targets. (3) The results of western blotting of HSP27, a-enolase, transferrin were consistent with the results of proteomic analysis. (4) STW may achieve therapeutic effects by interfering with the targets, biological processes and signaling pathways discovered in this study.
摘要：
目的:探讨寿胎丸对复发性流产模型大鼠蜕膜细胞水通道蛋白（AQP）1、2表达的影响。方法:取复发性流产模型大鼠胚胎着床部位的蜕膜组织进行原代和传代培养,分别用寿胎丸含药血清、雌激素（E2）及孕激素（P）进行干预,另设空白对照组,4组药物对培养的蜕膜细胞作用72h后,通过q RT-PCR及Western blot方法检测蜕膜细胞中AQP1、AQP2 m RNA及蛋白的表达。结果:与空白对照组比较,寿胎丸组及P组AQP1、AQP2 m RNA及蛋白的表达均显著提高（P<0.05）,E2组AQP1、AQP2 m RNA及AQP2蛋白的表达显著提高（P<0.01）。P比寿胎丸更显著上调AQP1 m RNA及蛋白的表达（P<0.01）。E2、P均较寿胎丸显著上调AQP2 m RNA的表达（P<0.01）。结论:寿胎丸可能通过上调复发性流产大鼠蜕膜组织AQP1、AQP2 m RNA及蛋白的表达水平,改善妊娠结局。寿胎丸对AQP蛋白的调节,说明"肾主水"与"肾主生殖"之间存在相关性。
摘要：
Objective To visualize Professor YOU Zhaoling's academic research by means of bibliometric tools.Methods“尤昭玲”was taken as the key word in the author’s search in Chinese National Knowledge Infrastructure(CNKI).Visual analysis of the literature was carried out by CiteSpace.The author’s publication number,author’s co-operative network,author cluster analysis,keyword co-occurrence network,keyword emergent words and keyword clustering were analyzed respectively.Results Professor YOU Zhaoling's main research directions were polycystic ovary syndrome,traditional Chinese medicine(TCM)supplemented treatment of in vitro fertilization-embryo transfer,infertility and premature ovarian failure.Professor YOU original created"YOU’s Eight Methods"and"Three Phases of Type Two Assisted Pregnancy"to treat polycystic ovarian syndrome,formulated"Six Phases and Seven Steps"TCM-assisted IVF-ET technique,and created"Iceberg Theory"and"Looking at the Eye to Identify the Ovary Method"to guide the treatment of premature ovarian failure.Conclusion Professor YOU,with the help of modern physiological and pathological technology and classical theory of TCM,explains the occurrence and development of diseases,and creatively combines traditional Chinese and western medicine to guide and treat gynecological diseases,thus forming a distinctive"YOU’s"diagnosis and treatment,which provides new ideas and methods for the modernization of TCM.
