作者机构：
Pathophysiology Laboratory,Hunan University of Chinese Medicine,Changsha,Hunan,P.R.China;Department of Histology and Embryology,Hunan University of Chinese Medicine,Changsha,Hunan Province,
摘要：
Astragalus and Panax notoginseng are often combined to treat cerebral infarction.Astragalosides (AST) and Panax notoginseng saponins (PNS) are the effective components with cardio-cerebrovascular pharmacological effects in Astragalus and Panax notoginseng,respectively,they both may antagonize ischemic brain injury.The purpose of this work was to compare the protective effects of AST,PNS and their combinations against cerebral ischemia injury through energy metabolism and neurocyte apoptosis.C56BL/6 mice were randomly divided into sham group,model group,AST (55 mg/kg) group,PNS (57.5 mg/kg)group,small-dose combination group (AST 27.5 mg/kg + PNS 28.75 mg/kg),moderate-dose combination group (AST 55mg/kg + PNS 57.Smg/kg),large-dose combination group (AST 110 mg/kg + PNS 115 mg/kg) and edaravone (4 mg/kg) group,treated for 4 days,then,cerebral ischemia-reperfusion injury was established by bilateral common carotid artery (CCA) ligation for 20 min followed by reperfusion.Our results showed that AST,PNS and their combinations increased the levels of ATP,ADP and Na(+)-K(+)-ATPase activity after reperfusion for 1 h.Twenty-four and 48 h after reperfusion,AST,PNS and the combinationssignificantly increased neurocyte survival and decreased the apoptosis rate as well as down-regulated the expression of phosphorylated c-June N-terminal kinasel/2 (p-JNK1/2),cytochrome C (Cyt C),cysteine aspartic acid-specific protease (caspase)-9 and caspase-3.Furthermore,the effects on energy metabolism and apoptosis were dose dependent among smalllow-dose combination group,moderate-dose combination group and large-dose combination group,and being better in moderate-dose combination group than those in AST or PNS group alone.It suggested that AST,PNS and their combinations had protective effects against cerebral ischemia injury,and the combination between AST and PNS could intensify the effect,the mechanism underlying might be associated with improving energy metabolism,and(_)reducing neurocyte apoptosis via the inhibitions of JNK signaling pathway and mitochondrial apoptosis pathway.
作者机构：
[Xiao-ping Huang; Hua Tan; Bei-yang Chen] Laboratory of Pathophysiology,Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China;[Chang-Qing Deng] Laboratory of Pathophysiology,Hunan University of Traditional Chinese Medicine, Changs
会议名称：
国际补充医学研究会第六届年会
会议时间：
2011-5-7
会议地点：
成都
会议主办单位：
国际补充医学研究会
会议论文集名称：
国际补充医学研究会第六届年会论文集
关键词：
Cerebral ischemia;Reperfusion damage;Astragalosides;Panax Notoginseng Saponins;matrix metalloproteinases-9;tissue inhibitor of metalloproteinase-1
摘要：
Aim: To investigate the effect of compatibility of Astragalosides (AST) with Panax Notoginseng Saponins (PNS) on the expression of matrix metalloproteinases-9（MMP-9） and tissue inhibitor of metalloproteinase-1 (TIMP-1) after cerebral ischemia reperfusion (CIR) injury in mice and probe its anti-ischemic brain injury protection mechanism.
摘要：
单磷酸腺苷（adenosine monophosphate,AMP）激活的蛋白激酶（AMP-activated protein kinase,AMPK）作为一种能量调控器,在脑缺血时能被激活,活化的AMPK对缺血后脑组织的物质和能量代谢、神经元的损伤和修复、血管和神经的再生等均可产生重要影响。开展AMPK对缺血性脑损伤作用的研究,有望为脑缺血性疾病的治疗提供一个新的方向和靶点。
