期刊：
European Journal of Gastroenterology and Hepatology,2018年30(2):130-135 ISSN：0954-691X
通讯作者：
Sun, Kewei;Lang, Ren
作者机构：
[Sun, Kewei; Wang, Ya; Zhang, Tao] Hu Nan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Huang, Lei] 302 Hosp PLA, Treatment & Res Ctr Infect Dis, Beijing, Peoples R China.;[Lang, Ren] Capital Med Univ, Dept Hepatobiliary Surg, Beijing Chaoyang Hosp, Beijing 10020, Peoples R China.;[Jiang, Wei; Zhang, Tao] Med Univ South Carolina, Dept Microbiol & Immunologys, Div Infect Dis, Charleston, SC USA.;[Jiang, Wei] Med Univ South Carolina, Dept Med, Div Infect Dis, Charleston, SC USA.
通讯机构：
[Sun, Kewei] H;[Lang, Ren] C;Hu Nan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;Capital Med Univ, Dept Hepatobiliary Surg, Beijing Chaoyang Hosp, Beijing 10020, Peoples R China.
摘要：
Acute-on-chronic liver failure (ACLF) is characterized by organ failure mediated by acute decompensation of cirrhosis. Recent studies have highlighted the importance of the gut-liver axis (GLS) and its association with ACLF pathogenesis. In this review, we discuss the mechanisms related to the alteration of the GLA and their involvement in ACLF pathogenesis and suggest some possible therapeutic options that could modulate the GLA dysfunction. This knowledge may provide information useful for the design of therapeutic strategies for gut dysbiosis and its complications in ACLF. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.
作者机构：
[常小荣; 邓凯文] Department of Acupuncture and Moxibustion, the First Affiliated Hospital of Hunan University of CM, Changsha 410007, China;[唐闻汉; 周逸群; 贺福元; 杨岩涛; 陶叶琴; 石继连; 刘文龙] College of Pharmaceutics, Hunan University of CM, Changsha 410007, China;[唐闻汉; 周逸群; 贺福元; 杨岩涛; 陶叶琴; 石继连; 刘文龙] Hunan Provincial Key Laboratory of Druggability and Preparation Modification of TCM, Changsha 410007, China;[贺福元] Key laboratory of Property and pharmacodynamic of TCM, State Administration of TCM, Changsha 410007, China;[周逸群; 贺福元; 杨岩涛; 石继连; 刘文龙] Supramolecular Mechanism and Mathematic-Physics Characterization for Chinese Materia Medica, Hunan University of CM, Changsha 410007, China
作者机构：
[Cai, Xiong; Yu, Rong; Song, Hou-Pan; Li, Ru-Yi; Huang, Hui-Yong; Li, Xin] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost & Therapeut Res Chine, Changsha 410208, Hunan, Peoples R China.;[Hou, Xue-Qin] Taishan Med Coll, Inst Pharmacol, Tai An 271000, Shandong, Peoples R China.;[Zhou, Sai-Nan] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Cai, Xiong] Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Guangzhou 510006, Guangdong, Peoples R China.;[Zhou, Chi] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Guangdong, Peoples R China.
通讯机构：
[Zhou, Chi; Cai, Xiong] G;Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Guangdong, Peoples R China.;Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Higher Educ Mega Ctr, 280 East Waihuan Rd, Guangzhou 510006, Guangdong, Peoples R China.
关键词：
Atractylenolide I;Cell migration and proliferation;Cytosolic free Ca(2+);Intestinal epithelial repair;Polyamine
摘要：
Background : An impairment of the integrity of the mucosal epithelial barrier can be observed in the course of various gastrointestinal diseases. The migration and proliferation of the intestinal epithelial (IEC-6) cells are essential repair modalities to the healing of mucosal ulcers and wounds. Atractylenolide I (AT-I), one of the major bioactive components in the rhizome of Atractylodes macrocephala Koidz. (AMR), possesses multiple pharmacological activities. This study was designed to investigate the therapeutic effects and the underlying molecular mechanisms of AT-I on gastrointestinal mucosal injury. Methods: Scratch method with a gel-loading microtip was used to detect IEC-6 cell migration. The real-time cell analyzer (RTCA) system was adopted to evaluate IEC-6 cell proliferation. Intracellular polyamines content was determined using high performance liquid chromatography (HPLC). Flow cytometry was used to measure cytosolic free Ca2+ concentration ([Ca2+](c)). mRNA and protein expression of TRPC1 and PLC-gamma(1) were determined by real-time PCR and Western blotting assay respectively. Results: Treatment of IEC-6 cells with AT-I promoted cell migration and proliferation, increased polyamines content, raised cytosolic free Ca2+ concentration ([Ca2+] c), and enhanced TRPC1 and PLC-gamma(1) mRNA and protein expression. Depletion of cellular polyamines by DL-a-difluoromethylornithine (DFMO, an inhibitor of polyamine synthesis) suppressed cell migration and proliferation, decreased polyamines content, and reduced [Ca2+] c, which was paralleled by a decrease in TRPC1 and PLC-gamma(1) mRNA and protein expression in IEC-6 cells. AT-I reversed the effects of DFMO on polyamines content, [Ca-2+] c, TRPC1 and PLC-gamma(1) mRNA and protein expression, and restored IEC-6 cell migration and proliferation to near normal levels. Conclusion: Our data demonstrate that AT-I stimulates intestinal epithelial cell migration and proliferation via the polyamine-mediated Ca2+ signaling pathway. Therefore, AT-I may have the potential to be further developed as a promising therapeutic agent to treat diseases associated with gastrointestinal mucosal injury, such as inflammatory bowel disease and peptic ulcer. (C) 2017 Elsevier GmbH. All rights reserved.
摘要：
Novel organobismuth(III) complex of 5H-dibenzo[c,f][1,5]oxabismocin-12(7H)-yl nitrate (C2) was synthesized and characterized by spectral and elemental analysis. It was compared with other five C,E,C-chelating (E = N, O, S) organobismuth(III) complexes against human adenocarcinoma alveolar basal epithelial cells (A549), human liver cancer cell line (SMCC7721), human gastric cancer cell line (SGC-7901), human colon adenocarcinoma cell line (SW480) and healthy human bronchial cell line (16HBE14o-) in vitro. It was found that C2 exhibited the best anticancer activity. Further mechanistic investigation indicated that toxicological activity of C2 was ascribable to apoptosis rather than anti-proliferative activity. Apoptosis was induced through up-regulating the level of Bcl-2/Bax as well as the activation of caspase-3. The results demonstrate that heterocyclic organobismuth(III) complexes of this type have great potential in the treatment of cancer.
摘要：
Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs—Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae—is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.
关键词：
hepatitis B virus;interferon;mutation;treatment efficacy
摘要：
BACKGROUND: Accumulating studies assessing the impacts of hot spot mutations on conventional interferon (IFN) efficacy come to discrepant conclusions; studies regarding the mutations in S and RT regions are also unclear. The present study aimed to evaluate the impacts of HBV mutations on the efficacy of conventional IFN. METHODS: A total of 126 patients who received conventional IFN treatment for 48 weeks were enrolled. Biochemical and serological parameters were routinely tested. The sequences of HBV from 78 serum samples were amplified by nested-PCR; mutations were identified with sequence scanner V1.0 after ABI 3730xl direct sequencing, HBV genotypes were determined according to RT gene sequences utilizing NCBI Genotyping Tool which was based on phylogenetic analysis. RESULTS: The baseline DNA levels of virological response (VR) group were significantly lower than those of no VR group [7.13±0.76 vs 7.69±0.56 lg (copies/mL), P=0.001]. The baseline ALT levels were significantly higher in the HBeAg clearance group (204.72±88.65 vs 162.80±85.81 IU/L, P<0.05) and HBeAg seroconversion group (204.89 ±95.68 vs 166.75±84.43 IU/L, P<0.05). Females and lower BMI levels (20.01±2.33 vs 21.65±3.66 kg/m~2, P<0.05) were prone to acquired biochemical response (BR). PC-W28STOP (ntG1896A) was significantly higher in the combined response (CR) group than that in the no CR group (91.7% vs 39.7%, P=0.001). Multivariate logistic regression analysis showed that baseline DNA, PC-P159T (nt- C2288A), BCP-N118T (ntA1726C) and BCP-L134L (ntA1775C/ G/T) influenced VR independently. PC-G182C (ntG2357T), PC-S64A/T (ntT2003G/A) and BMI were independent influence factors for HBeAg clearance, HBeAg seroconversion and BR, respectively. The new predicting model concluded that baseline DNA and new mutations for VR were established successfully, and ROC analysis showed that AUC was 0.842 (P<0.001) with a sensitivity of 0.652 and a specificity of 0.933. CONCLUSIONS: PC-P159T (ntC2288A), BCP-N118T (ntA1726C), BCP-L134L (ntA1775C/G/T), PC-G182C (ntG2357T) and PCS64A/ T (ntT2003G/A) were novel identified mutations that impacted IFN therapeutic efficacy. These novel mutations could serve as important predictors before conventional IFN treatment.
作者机构：
[魏歆然] The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[魏高文] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[文琪琦; 刘丽; 谢莉娜; 吴雪芬; 陈小丽; 郑雪娜; 谢志强] College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[岳增辉] College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. 624755064@qq.com
作者机构：
[潘江] Hunan University of CM, Changsha 410007, China;[章薇; 娄必丹; 潘江] Department of Acupuncture and Moxibustion;[林碧莲] College of International Education, Hunan University of CM;[陈明] Department of Stomatology, the First Affiliated Hospital of Hunan University of CM, Changsha 410007
作者：
Luo Xiaodong*;Wang Peng;Li Zhe;Liang De;Huang Feng;...
期刊：
中医杂志,2017年37(2):193-200 ISSN：1001-1668
通讯作者：
Luo Xiaodong
作者机构：
[Luo Xiaodong; Li Zhe] Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Guangzhou 510370, Guangdong, Peoples R China.;[Wang Peng] Macau Univ Sci & Technol, Fac Chinese Med, Macau 999078, Peoples R China.;[Huang Feng; Liang De] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Dept Orthopaed, Guangzhou 510405, Guangdong, Peoples R China.;[Liu Jinwen] Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Orthopaed, Guangzhou 510120, Guangdong, Peoples R China.;[Chen Xinyu] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Cardiol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Luo Xiaodong] G;Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Guangzhou 510370, Guangdong, Peoples R China.
关键词：
Spondylosis;Deficiency of liver and kidney;Qi-deficiency blood stasis;Jingfukang granules;Randomized controlled trial
摘要：
OBJECTIVE: To evaluate the effectiveness and safety of cervical spondylosis formula granules in reducing the symptoms of patients with the nerve root type and the vertebral artery type of cervical spondylosis. METHODS: This was a multicenter, single-blind, randomized, controlled trial. From April 2002 to November 2003, 499 patients were randomly assigned to either the treatment or the control group. The treatment group was orally administered granules prepared with a formula for cervical spondylosis, while the control group was given Jingfukang granules. The treatment course was 1 month for both groups. RESULTS: In patients with the nerve root type of cervical spondylosis, the total effect rate in the treatment group (87.21% ) was significantly higher than that in the control group (80.70%, P < 0.01). After the treatment period in both groups, the treatment group had a significantly greater rate of resolution of pain, numbness of the upper limbs, muscle strength of the upper limbs, and fatigue than the control group (all P < 0.05). In patients with the vertebral artery type of cervical spondylosis, the total effect rate in the treatment group (82.07%) was similar to that in the control group (71.21% , P > 0.05). After the treatment period in both groups, the treatment group had a significantly greater rate of resolution of weakness of the waist and knees than the control group (P < 0.05). CONCLUSION: The cervical spondylosis formula granules significantly improve numbness, muscle strength, and fatigue, and reduce pain in patients with the nerve root type of cervical spondylosis, and improve the weakness of the waist and knees in patients with the vertebral artery type of cervical spondylosis.
