作者机构：
[谢文娟; 刘密; 林海波; 谭成富; 贺香嫦; 常小荣; 阳晶晶] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[严洁] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. yj5381159@126.com;[王超] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. 592436380@qq.com
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com
关键词：
Moxibustion;Point ST 21 (Liangmen);Point ST 36 (Zusanli);Stomach ulcer;Apoptosis;Cell proliferation
摘要：
OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen (ST 21) and Zusanli (ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer. METHODS: Forty rats were randomly divided into four groups: negative control (NC), ulcer control (UC), acupoints of stomach meridian (ASM), and acupoints control (AC). The acute gastric ulcer model was established by bound and water immersion. Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen (ST 21) and Zusanli (ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method. The expression of tumor necrosis factor-alpha (TNF-α), apoptotic protease activating facter-1 (Apaf-1), Caspase-3, p21 activated kinase 1 (PAK1), extracellular regulated protein kinases 2 (ERK2), phosphorylated ERK2 (pERK2), phosphoinositide 3-kinase (PI3K) and RAC-alpha serine/threonine- protein kinase (Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced (P < 0.05), while the expression of PAK1, ERK2, pERK2, PI3K and Akt in gastric mucosa was significantly increased (P < 0.05). And ASM showed better effect than AC group (P < 0.05). CONCLUSION: Moxa-burning Heat stimulating of Liangmen (ST 21) and Zusanli (ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.
摘要：
Sensory inputs stimulated by Zusanli (ST36) acupuncture in the abdomen are known to converge in the upper cervical cord. However, it is unclear whether these inputs are subsequently conveyed to the hypothalamic paraventricular nucleus and what kind of afferent fibers are involved. We focused on the upper cervical cord, where afferent inputs converge, and detected c-fos expression in oxytocinergic neurons. We found that Zusanli acupuncture therapy effectively elevated intragastric pressure, but inhibited expression of c-fos in oxytocinergic neurons of the paraventricular nucleus in upper cervical cord injured rats. These Zusanli acupuncture effects remained even after complete dorsal cord transection. However, after complete transection of the spinal cord or dorsolateral funiculus, the effects were significantly attenuated and even disappeared. These findings suggest that the paraventricular nucleus is responsible for pooling and integrating signals from the Zusanli acupuncture and sensory information from the intragastric pressure variation, thereby contributing to the regulation of intragastric pressure. The upper cervical cord serves as the key link between ascending and descending pathways, which conveys afferent inputs to the paraventricular nucleus through the dorsolateral funiculus.
作者机构：
[李玲; 陈沙; 刘慧萍; 喻嵘] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[谭佳佳; 张朴真; 吴倩] College of Acupuncture and Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, 410208, China
作者机构：
[Tang, Qing-ping] Hunan Univ Chinese Med, Brain Hosp Hunan Prov, Dept Rehabil, Changsha 410007, Hunan, Peoples R China.;[Tang, Qing-ping; Wu, Li-xiang] Cent S Univ, Sch Basic Med Sci, Dept Physiol, Changsha 410078, Hunan, Peoples R China.;[Shen, Qin] Cent S Univ, Xiangya Hosp, Dept Neurol, Changsha 410008, Hunan, Peoples R China.;[Feng, Xiang-ling; Liu, Hui] Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Hunan, Peoples R China.;[Wu, Bei] Cent S Univ, Xiangya Hosp, Dept Otolaryngol, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Liu, Zun-jing] C;China Japan Friendship Hosp, Dept Neurol, Beijing 100029, Peoples R China.
关键词：
Motor training;Signal transducer and activator of transcription 3 (STAT3);Brain-derived neurotrophic factor (BDNF);Protein interacting with C kinase 1 (PICK1);Neural plasticity
摘要：
Willed-movement training has been demonstrated to be a promising approach to increase motor performance and neural plasticity in ischemic rats. However, little is known regarding the molecular signals that are involved in neural plasticity following willed-movement training. To investigate the potential signals related to neural plasticity following willed-movement training, littermate rats were randomly assigned into three groups: middle cerebral artery occlusion, environmental modification, and willed-movement training. The infarct volume was measured 18 d after occlusion of the right middle cerebral artery. Reverse transcription-polymerase chain reaction (PCR) and immunofluorescence staining were used to detect the changes in the signal transducer and activator of transcription 3 (STAT3) mRNA and protein, respectively. A chromatin immunoprecipitation was used to investigate whether STAT3 bound to plasticity-related genes, such as brain-derived neurotrophic factor (BDNF), synaptophysin, and protein interacting with C kinase 1 (PICK1). In this study, we demonstrated that STAT3 mRNA and protein were markedly increased following 15-d willed-movement training in the ischemic hemispheres of the treated rats. STAT3 bound to BDNF, PICK1, and synaptophysin promoters in the neocortical cells of rats. These data suggest that the increased STAT3 levels after willed-movement training might play critical roles in the neural plasticity by directly regulating plasticity-related genes.
摘要：
语言治疗是对各种语言障碍和交流障碍进行评价、治疗和研究的学科。美国听力语言协会(American Speech-Language and Hearing Association, ASHA)将此专业正式定名为言语-语言病理学,其专业人员称为言语-语言病理学家(speech-language pathologist, SLP),在我国从业人员多称为语言治疗师或言语治疗师。基于我国目前语言治疗师严重匮乏的情况,湖南省康复医学会言语治疗专业委员会与中国国际言语语言听力协会(Chinese International Speech-Language and Hearing Association, CISHA)共同发起了为期6个月的“全国在岗语言治疗人员普查”工作,调查范围包括东部地区的黑龙江、吉林、辽宁、河北、山东、江苏、安徽、浙江、福建、广东、北京、天津、上海、海南;中部地区的河南、山西、湖南、湖北、江西、内蒙古;西部地区的陕西、甘肃、四川、青海、贵州、云南、新疆维吾尔自治区、宁夏回族自治区、广西壮族自治区、重庆,共计30个省市,涵盖医疗、残联、民政、高校这四大系统的语言治疗师,是较为全面的一次调查。现调查工作已圆满结束,现将调查结果报道如下。
作者机构：
[陈伟; 李成文] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China;[端木程琳; 陈淑萍; 王俊英] Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700;[常小荣] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China. xrchang1956@163.com;[刘俊岭] Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700. 13521898023@163.com
摘要：
OBJECTIVE: To design a combined dynamic inhalation device for testing the toxicity induced by moxa smoking.METHODS: The new apparatus (Patent No.201120101911.5) includes air renewal and recycling systems, a gas generating device, a gas control unit, and a device to measure and control temperature and humidity. Sprague-dawley rats were tested for acute and sub-chronic toxicity after exposure to moxa-burning smoke.RESULTS: We found an LC_(50) of 1.2 × 10~4 mg/m~3 in the acute toxicity assays. In sub-chronic toxicity tests the organ coefficients studied showed no significant differences within rats groups of the same gender after treatment with moxa smoke or a month of recovery. However, mean gray degree of lung 70 heat shock protein (HSP70) was significantly elevated in the high dose group in comparison with the low dose group (P < 0.05); mean gray degree, mean optical density, gross area of HSP70 in other organs and caspase-9 parameters showed no significant differences between groups.CONCLUSION: These results suggest that moxa smoke had no overt toxicity in rats. This work provides evidence and reference for the design of dynamic inhalation exposure systems.
作者机构：
[Hong ZHANG; Xiqin YI; Xi LING; Jinfeng WU; Kun AI] College of Acu-Moxibustion and Tuina, Hunan University of TCM, Changsha 410208, Hunan Province, China
关键词：
电针;下合穴;UC模型大鼠;白介素-1β;肿瘤坏死因子-α;高迁移率族蛋白;合治内府
摘要：
Objective To comparatively observe the effect of electroacupuncture at digestive system-related lower he-sea points on the expressions of serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) of colon tissues and high mobility group box 1 protein (HMGB 1) of ulcerative colitis (UC) model rats, and to explore whether there is relative specificity of electroacupuncture at Shangjuxu (上巨虚 ST 37), one of lower he-sea points of large intestine, in treatment of bowel diseases. Method A total of 60 SD rats were randomly divided into control group, model group, ST 37 group, Zusanli (足三里 ST 36) group, Xiajuxu (下巨虚 ST 39) group and Yanglingquan (阳陵泉 GB 34) group. There were ten rats in each group; five were males, and five were females. UC models were established by clysis with 2, 4, 6-trinitrobenzene sulfonic acid/alcohol solution. After modeling, treatment was conducted for ten days, specimens were collected, colonic ulcers and inflammation were inspected visually and scored. The content of serum IL-1β and the expressions of TNF-α and HMGB 1 in colon were detected through ELISA. Results ① Compared with control group, the scores of colonic ulcers and inflammation, the content of serum IL-1β and the expressions of TNF-α (except ST 37 group) and HMGB 1 were all higher (P<0.05, P<0.01); ② compared with model group, the scores of colonic ulcers in ST 36 group and ST 37 group were lower obviously (P<0.05, P<0.01); the expressions of IL-1β, TNF-α and HMGB 1 in the four treatment groups were lower obviously (P<0.01); ③ compared with ST 37 group, the expressions of IL-1β, TNF-α and HMGB 1 in other three treatment groups were higher obviously (P<0.05, P<0.01); and the scores of colonic ulcers in ST 39 group and GB 34 group were higher obviously (P<0.05). Conclusion ① The score of colonic ulcers can be reduced through electroacupuncture at ST 37, ST 36, ST 39 and GB 34, which can also reduce the content of serum IL-1β and the expressions of TNF-α and HMGB 1, and effectively inhibit inflammatory response of colon caused by UC; ② the effect trend of the four acupoints in treatment of UC is: ST 37>ST 36>ST 39>GB 34, and electroacupuncture at ST 37 has the best effect with relative specificity.
作者机构：
[Wu Xiaqiu; Peng Jin] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.;[Li Guoqin] China Acad Chinese Med Sci, Guanganmen Hosp, Dept Resp, Beijing 100700, Peoples R China.;[Zhang Wei] Hunan Univ Chinese Med, Hosp 1, Dept Acupuncture & Massage, Changsha 410000, Hunan, Peoples R China.;[Liu Guangxia] Anhui Univ Chinese Med, Hosp 2, Dept Acupuncture, Hefei 230000, Peoples R China.;[Liu Baoyan] China Acad Chinese Med Sci, Beijing 100700, Peoples R China.
通讯机构：
[Peng Jin] C;China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.
关键词：
Acupoint sticking therapy;Quality of Life;Self efficacy;Asthma;Chinese medicine, traditional
摘要：
OBJECTIVE: To evaluate the self-efficacy and satisfaction of asthma patients subjected to summer acupoint application treatment (SAAT). METHODS: A two-year follow-up clinical study was conducted. Patients with asthma were treated by applying a herbal paste onto the Feishu (BL 13) and Fengmen (BL 12) acupoints on the three hottest days of summer, according to the traditional Chinese calendar, from 2008 to 2010. During a two-year follow-up, these patients were asked to fill out a questionnaire evaluating asthma degree, exacerbation frequency, concomitant medications and self-satisfaction. The self-rate and doctor-report outcomes obtained in parallel were evaluated to assess the efficacy of SAAT. RESULTS: A total of 527 asthma patients were initially enrolled in this study, of which 97 elderly patients and those with more severe cases of asthma were lost to follow-up. Thus, a total of 430 patients were valid for analysis using self-rate data. Nevertheless, occasional negative returns were obtained; almost all of the outcomes were rated as "No change", "Moderate effective", or "Very effective". In addition, 80% of the patients were satisfied with this treatment. Moreover, 391 (91.4%) patients were somewhat improved after SAAT in 2009, and further improvement was observed in 2010. After SAAT, the average asthma-degree score decreased from 5.3 in 2008 to 4 in 2009 and, subsequently to 3.5 in 2010. CONCLUSION: With pronounced patient satisfaction, SAAT can reduce the exacerbation severity and frequency, concomitant medications and asthma degree. Prolonging the treatment course might enhance the efficacy of SAAT. (C) 2015 JTCM. All rights reserved.
作者机构：
[刘密; 汪厚莲; 常小荣; 刘迈兰; Guo A.-L.; 李成文] College of Acupuncture and Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, 410007, China
通讯机构：
[Chang, X.-R.] C;College of Acupuncture and Moxibustion and Tui-na, Hunan University of Chinese Medicine, Changsha, China
关键词：
电针;高脂血症;Meta 分析;动物实验
摘要：
目的系统评价电针对高脂血症动物模型的有效性和安全性。方法计算机检索 CNKI、WanFang Data、VIP、CBM、PubMed、EMbase 和 The Cochrane Library(2013年12期),查找电针治疗高脂血症的相关动物实验,检索时限截至2013年12月。由2位研究者独立地按照纳入与排除标准筛选文献、提取资料,然后采用改良后的 CAMARADES 评分清单进行质量评价。采用 RevMan 5.2软件进行 Meta 分析。结果最终纳入4篇文献,包括89只大鼠,CAMARADES 清单平均评分为4分。Meta 分析结果显示:在总胆固醇降低[MD=0.06,95%CI(–0.08,0.20),P=0.40]、甘油三酯降低[MD= –0.01,95%CI(–0.08,0.06),P=0.74]、低密度脂蛋白胆固醇降低[MD=0.01,95%CI(–0.04,0.03),P=0.65],以及高密度脂蛋白胆固醇降低方面[MD= –0.00,95%CI(–0.09,0.08),P=0.93],电针组与药物组疗效差异均无统计学意义。结论现有有限证据显示,电针在降低高脂血症大鼠的总胆固醇、甘油三酯、低密度脂蛋白胆固醇和升高高密度脂蛋白胆固醇方面疗效与药物无差异。但受纳入研究样本量所限,该结论尚需更多高质量实验验证。
摘要：
Previous studies have shown that somatic sensation by acupuncture and visceral nociceptive stimulation can converge in the nucleus tractus solitarii where neurons integrate signals impacting on the function of organs. To explore the role of the nucleus tractus solitarii in the protective mechanism of pre-moxibustion on gastric mucosa, nucleus tractus solitarii were damaged in rats and pre-moxibustion treatment at the Zusanli (ST36) point followed. The gastric mucosa was then damaged by the anhydrous ethanol lavage method. Morphological observations, enzyme linked immunosorbent assays, and western immunoblot analyses showed that gastric mucosa surface lesion and the infiltration of inflammatory cells were significantly ameliorated after pre-moxibustion treatment. Furthermore, the gastric mucosal damage index and somatostatin level were reduced, and epidermal growth factor content in the gastric mucosa and heat-shock protein-70 expression were increased. These results were reversed by damage to the nucleus tractus solitarii. These findings suggest that moxibustion pretreatment at the Zusanli point is protective against acute gastric mucosa injury, and nucleus tractus solitarii damage inhibits these responses. Therefore, the nucleus tractus solitarii may be an important area for regulating the signal transduction of the protective effect of pre-moxibustion on gastric mucosa.
作者机构：
[初晓; 李江山; Chen S.; 谭程; 张超; 雍春燕; 陈姝; 叶兰] College of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha, Hunan Province, 410007, China
通讯机构：
[Li, J.-S.] C;College of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha, Hunan Province, China
作者机构：
[李景兴] Rehabilitation Center for Children, Hengyang Central Hospital, Hengyang 421000, Hunan Province, China;[艾坤; 张雨辰; 匡静之; 张泓; 鲍秋影] College of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
通讯机构：
[Zhang, H.] C;College of Acupuncture, Moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
