通讯机构：
[Chang, Xiaorong] H;Hunan Univ Chinese Med, Coll Acupuncture & Tuina, Changsha 410007, Hunan, Peoples R China.
关键词：
pre-moxibustion;transection of neural pathway;Zusanli (ST36);gastric mucosal lesion;heat shock proteins-70;ulcer index;traditional Chinese medicine;neural regeneration
摘要：
Studies have shown that pre-moxibustion protects the gastric mucosa by up-regulating the expression of heat shock protein 70. However, the signaling pathway underlying this effect remains unclear. Rats were intragastrically administered absolute alcohol, causing obvious lesion of the gastric mucosa. Following pre-moxibustion at Zusanli (ST36) for 8 days, the ulcer index decreased to different degrees. The results of an enzyme linked immunosorbent assay and western blotting showed significant upregulation of heat shock protein 70 expression in the gastric mucosa and serum. None out of transection of the spinal cord, damage to the nucleus of the solitary tract, neurotomy of the vagal nerve and neurotomy of the common peroneal nerve affected the decrease in ulcer index or the increase in heat shock protein 70 expression in serum after pre-moxibustion at Zusanli, and heat shock protein 70 expression was obviously decreased in the gastric mucosa. These findings suggest that pre-moxibustion at Zusanli can protect the gastric mucosa against lesioning, and that the mechanism underlying this effect involves its induction of heat shock protein 70 expression. Neural pathways participate in the regulatory effects of moxibustion on heat shock protein 70 expression in the gastric mucosa.
作者机构：
Acupuncture and Massage College, Hunan University of Traditional Chinese Medicine, Changsha 410208, Hunan Province, China;In recent years, many scholars have done a lot of studies on pathological features of chronic bronchitis based on animal model construction to further elucidate the genetic background of the incidence, predisposing factors, and pathogenesis.;To summarize the investigative situation and progression about animal models of chronic bronchitis at home and abroad.;Databases of CNKI and PubMed (1999-01/2011-10) were searched to retrieve the related articles about animal models of chronic bronchitis using the key words of “chronic bronchitis, animals, model” in Chinese and English, respectively. Finally, 24 articles were included in result analysis.;The methods to construct an animal model of chronic bronchitis mainly include chemical medicine, bacterial infection, passive smoking and composite method. Each method has its own advantages and disadvantages. Up to now, there is no recognized ideal method that needs further studies.
通讯机构：
College of Acupuncture-Moxibustion and Massage, Hunan University of TCM, Major Laboratory of Meridians and Viscera, Tertiary Laboratory of State Administration of TCM, Changsha 410007, China.,
摘要：
A middle cerebral artery occlusion-model was established in rats using the improved thread embolism method.Rats were treated with acupuncture at either Dazhui(DU14),Renzhong(DU26), Baihui(DU20),or a non-meridian point.Detection with protein-chip technology showed that the level of protein phosphorylation in both groups was upregulated or downregulated depending on the signaling pathway compared with the model group that did not receive acupuncture.Analysis of proteins showing downregulated phosphorylation revealed that five signaling pathways were activated in the acupuncture-treatment group,while only two were activated in the acupuncture-control group.In contrast,analysis of proteins showing upregulated phosphorylation revealed only one pathway was activated in the acupuncture-treatment group,whereas four pathways were activated in the acupuncture-control group.Furthermore,the number of activated proteins in the acupuncture-treatment group was not only higher than the acupuncture-control group,but unlike the acupuncture-control group,the majority of activated proteins were key proteins in the signaling pathways.Our findings indicate that acupuncture at specific points can activate multiple signaling pathways to promote the restoration of brain tissue following ischemic injury,and that this is based on a combination of effects resulting from multiple pathways,targets,and means.
摘要：
This study compares the changes of DNA repair in brain tissue dominated by the middle cerebral artery after electroacupuncture at the acupoints of Renzhong (DU 26), Neiguan (PC 6), Quchi (LI 11) and Zusanli (ST 36) in rats. In the apurinic/apyrimidinic endonuclease DNA basic group reparative excision experiments, the apurinic/apyrimidinic endonuclease activity of brain tissue increased slightly after electroacupuncture in rats. In the DNA polymerase β (Pol β)experiments, the Pol β activity of brain tissue increased after electroacupuncture, especially at DU 26 and PC 6. In the DNA ligase experiments, the expression of DNA ligase 1 in brain tissue increased significantly after electroacupuncture. These findings demonstrate increased activity of apurinic/apyrimidinic endonuclease, Pol β and DNA ligase 1 after electroacupuncture at DU 26 and PC 6. Also, DNA repair in brain tissue supplied by the middle cerebral artery is promoted after electroacupuncture at DU 26 and PC 6, which are more effective than the LI 11 and ST 36 acupoints
通讯机构：
Lab for Studying Meridian-Zangfu-organ Correlation, College of Acu-moxibustion and Massage, Hunan University of Chinese Medcine, Changsha 410007, China.,
