摘要：
Ethnopharmacological relevance: Huang-Qi-Jian-Zhong-Tang (HQJZT) is a canonical traditional Chinese medicine (TCM) formula that has been widely used in both the prevention and treatment of gastrointestinal diseases, including gastric ulcer, duodenal ulcer, and chronic atrophic gastritis, in China. Aim of the study: In the present study, we investigated the gastroprotective potential of HQJZT in a rat model of indomethacin (IND)-induced gastric ulcer and explained the biochemical, cellular, and molecular mechanisms involved. Materials and methods: Observations were conducted at the macroscopic level to ascertain the ulcer index (UI) and the curative index (CI). Histopathological examinations were conducted, and a microscopic score (MS) was computed. The gastric juice volume, total acidity, pH value, and pepsin activity were quantified. Antioxidant and oxidative parameters were assessed, namely GSH, CAT, SOD, and MDA content. The RFLSI Pro instrument was employed to measure the blood flow within the gastric mucosa continuously. The mRNA levels of the inflam-matory cytokines were assessed using droplet digital PCR (ddPCR). Molecular docking was employed to examine the interaction between representative active components of HQJZT and the binding sites associated with the NF-kappa B and STAT signaling pathways. The protein expression and localization of p-JAK, p-STAT, p-I kappa B beta, and p-NF-kappa B were evaluated through immunofluorescence analysis. Results: The administration of HQJZT treatment demonstrated a significant reduction in gastric lesions induced by IND, leading to a notable decrease in the UI. Additionally, HQJZT treatment significantly decreased gastric juice volume, acidity, and pepsin activity, accompanied by increased pH value. IND-treated stomachs exhibited severe hemorrhagic necrosis, submucosal edema, and epithelial cell destruction. However, the administration of HQJZT effectively counteracted these pathological changes. Furthermore, HQJZT administration significantly increased blood flow to the gastric mucosa. HQJZT enhanced antioxidant defenses and modulated oxidative stress by increasing SOD, CAT, and GSH activities while reducing MDA levels. Moreover, HQJZT reversed IND-induced increases in mRNA expression levels of inflammatory cytokines. Molecular docking analysis revealed that the representative active components of HQJZT could bind to the NF-kappa B and STAT signaling pathways. In addition, immunofluorescence microscopy revealed that HQJZT markedly attenuated the phosphorylation of I kappa & Vcy;beta, NF-kappa B, JAK, and STAT. Conclusions: The therapeutic and protective effect of HQJZT on gastric ulcers is attributed to its ability to suppress gastric acid secretion, enhance antioxidative defenses and blood flow, mitigate proinflammatory cytokines, and inhibit the activation of NF-kappa B and STAT signaling pathways.
期刊：
Frontiers in Cellular and Infection Microbiology,2023年13:1257817 ISSN：2235-2988
通讯作者：
Song, HP;Cai, X
作者机构：
[Song, Houpan; Sun, Qifang; Zeng, Meiyan] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Song, Houpan; Yuan, Chengzhi; Sun, Qifang] Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med TCM Diagnost, Changsha, Hunan, Peoples R China.;[Yuan, Chengzhi; Lu, Jing] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Zhou, Sainan] First Hosp Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Cai, Xiong; Cai, X] Hunan Univ Chinese Med, Sch Int Educ, Changsha, Hunan, Peoples R China.
通讯机构：
[Song, HP ; Cai, X ] H;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med TCM Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Int Educ, Changsha, Hunan, Peoples R China.
摘要：
Helicobacter pylori, a gram-negative microaerophilic pathogen, causes several upper gastrointestinal diseases, such as chronic gastritis, peptic ulcer disease, and gastric cancer. For the diseases listed above, H. pylori has different pathogenic mechanisms, including colonization and virulence factor expression. It is essential to make accurate diagnoses and provide patients with effective treatment to achieve positive clinical outcomes. Detection of H. pylori can be accomplished invasively and noninvasively, with both having advantages and limitations. To enhance therapeutic outcomes, novel therapeutic regimens, as well as adjunctive therapies with probiotics and traditional Chinese medicine, have been attempted along with traditional empiric treatments, such as triple and bismuth quadruple therapies. An H. pylori infection, however, is difficult to eradicate during treatment owing to bacterial resistance, and there is no commonly available preventive vaccine. The purpose of this review is to provide an overview of our understanding of H. pylori infections and to highlight current treatment and diagnostic options.
作者机构：
[Wu, Yaohui; Li, Jiaxin; Hu, Yuda; Wang, Yonghong; Zhou, Xuan] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;[Ning, Ge; Ning, G] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.
通讯机构：
[Wang, YH ] C;[Ning, G ] H;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.
摘要：
Kanamycin is used widely in livestock farming due to its antimicrobial properties and low cost, but has led to antibiotic residues in food, which can damage human health. Therefore, there is an urgent need for convenient technology that can be used to detect kanamycin rapidly. We found that Co(3)O(4) nanoparticles (NPs) possessed peroxidase-like activity that catalyzed the oxidation of 3,3',5,5'-tetramethylbenzidine to change color. Interestingly, a target-specific aptamer could regulate the catalytic activity of Co(3)O(4) NPs and inhibit this effect through aptamer-target binding. On the basis of a colorimetric assay combined with an aptamer-regulatory mechanism, the linear range for quantitative detection of kanamycin was 0.1-30 μM, the minimum limit of detection was 44.2 nM, and the total time needed for detection was 55 min. Moreover, this "aptasensor" displayed excellent selectivity and could be applied to detect KAN in milk samples. Our sensor might have promising applications for kanamycin detection in animal husbandry and agricultural products.
摘要：
Traditional methods for detecting antibiotic and mycotoxin residues rely on large-scale instruments, which are expensive and require complex sample pretreatment processes and professional operators. Although aptamer-based electrochemical sensors have the advantages of simplicity, speed, low cost, and high sensitivity, most aptamer-based sensors lack a signal amplification strategy due to their direct use of aptamers as probes, resulting in insufficient sensitivity. To solve the sensitivity problem in the electrochemical detection process, a novel electrochemical sensing strategy was established for ultrasensitive zearalenone (ZEN) detection on the basis of exonuclease I (Exo I) and branched hybridization chain reaction (bHCR) to amplify the signal. The amplification strategy showed excellent analytical performance towards ZEN with a low detection limit at 3.1×10(-12) mol/L and a wide linear range from 10(-11) to 10(-6) mol/L. Importantly, the assay was utilized in the corn powder samples with satisfactory results, holding promising applications in food safety detection and environmental monitoring.
期刊：
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2023年286:121953 ISSN：1386-1425
通讯作者：
Yonghong Wang<&wdkj&>Ge Ning
作者机构：
[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;[Liu, Yan; Guan, Baibing; Wu, Yaohui; Xu, Ziqi; Wang, Yonghong] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.;[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Peoples R China.;[Liu, Yan] South China Univ Technol, Sch Biol & Biol Engn, Guangzhou 510006, Peoples R China.
通讯机构：
[Yonghong Wang] H;[Ge Ning] I;Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004 Changsha, China<&wdkj&>International Education Institute, Hunan University of Chinese Medicine, 410208 Changsha, China
摘要：
Kanamycin was a group of essential antibiotics generally served in treating infections of animals which leached into the environment residual in food, causing health concerns. Thus, selective and sensitive monitoring of kanamycin was significant for food safety. In this work, split aptamers were used as templates to prepare fluorescent Cu/Ag NCs for detection of kanamycin. According to the impressive affinity of the aptamer to kanamycin, two different detection modes were designed using kanamycin aptamer as a recognition molecule, in which one was to combine split aptamer Apt-1 with Apt-2 to form an entangled DNA as a Cu/Ag NCs template, the other was to associate the normal aptamer after encirclement to form Cu/Ag NCs templates. After the addition of kanamycin, the fluorescence signals of the Cu/Ag NCs synthesized in the two modes were both enhanced, but the approach with split aptamer exhibited a superior observable sensitivity than that of the normal type. The detection range showed a well linear relationship between 80nM and 10μM when the emission wavelength was 560nm, and the detection limit was 13.3nM. In addition, when streptomycin, oxytetracycline, chloramphenicol and chlortetracycline were involved in the selective interference experiment under the same conditions, the fluorescence intensity of the system performed no significant changes. The results demonstrated that this method possessed favorable specificity and selectivity for the assay of kanamycin, proficiently achieving efficient, rapid and sensitive evaluation of kanamycin in the milk samples.
期刊：
Journal of Fluorescence,2022年32(5):1949-1957 ISSN：1053-0509
通讯作者：
Yonghong Wang
作者机构：
[Li, Hong; Xie, Chuchu; Wu, Yaohui; Yao, Liu; Li, Xin; Liao, Zhibin; Wang, Yonghong] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Ning, Ge] Hunan Univ Chinese Med, Int Educ Inst, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Yonghong Wang] H;Hunan Province Key Laboratory for Forestry Biotechnology, College of Life Science and technology, Central South University of Forestry and Technology, Hunan Province, Changsha, China
作者机构：
[Liao, Zhibing; Yao, Liu; Liu, Yan; Wu, Yaohui; Wang, Yonghong] Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha;410004, China;[Ning, Ge] International Education Institute, Hunan University of Chinese Medicine, Changsha;410208, China;[Liao, Zhibing; Yao, Liu; Liu, Yan; Wu, Yaohui; Wang, Yonghong] 410004, China
通讯机构：
[Yonghong Wang] H;[Ge Ning] I;Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China<&wdkj&>International Education Institute, Hunan University of Chinese Medicine, 410208, Changsha, China
摘要：
Herein, an effective pyrene excimer signaled fluorescent biosensor for the determination of tetracycline based on triple-helix aptamer probe (TAP) and supramolecular inclusion of cyclodextrin was reported. The TAP was devised containing an aptamer loop, two DNA segment stems and a triplex-forming oligonucleotide (signal probe) labeled with pyrenes at 5′ and 3′ ends. The presence of target could result in its binding towards aptamer with a mighty affinity, leading to a conformation change of the TAP and whereupon the release of the signal probe. This liberty of signal probe enabled the formation of pyrene excimer, generating fluorescence signals. Further, signal amplification was fulfilled through the addition of γ-cyclodextrin which could interact with pyrene dimer, thus leading to an enhanced “on-state” of the sensing ensemble. In contrast, when the target was absent, the sensing ensemble remained “off-state” because of the long distance between two pyrene molecules. When the conditions were properly optimized, the increasing signal kept a linear dependence on target concentrations ranging from 5.0nM to 100nM, and the detection limit reached as low as 1.6nM. In this way, a newly-constructed, simple, and economically affordable protocol enjoys desirable efficiency, sensitivity, specificity in biosensing. Also, its universality as another attractive behalf in assaying diverse targets was envisioned with only the need of matched aptamer replacement.
摘要：
Based on molecular imprinting and nanotechnology, a highly specific and sensitive electrochemical aptasensor was proposed for assaying kanamycin (KAN). First, chitosan-graphene (CG) composite multilayer films and Au nanoparticles (AuNPs) were applied to modify a glassy carbon electrode (GCE) for augmented "electron antennae". Then, we constructed a KAN sensor using functional monomer of 3-aminophenylboronic acid (3-APBA) as trap. Au@Fe3O4 nanocomposites, Fe3O4 nanoparticles (Fe(3)O(4)NPs) loaded with AuNPs, were dual functionally modified with beta-cyclodextrin-ferrocene (Fc/beta-CD-SH) and KAN aptamers (APT/Fc/beta-CD-SH/Au@Fe3O4) were applied as the tracing tag. The KAN aptamer acted as the bind unit to specifically identify the KAN captured by the imprinted cavities on the MIP modified electrode surface, while the Fc/beta-CD-SH was used as a signal unit for KAN quantification. The presented assay exhibited a good linear relationship between KAN concentration (10-500 nM) and the strength of the electrochemical signal, with a detection limit of 1.87 nM and a correlation coefficient of 0.98. The selectivity, stability and reproducibility of the proposed sensor were acceptable. Furthermore, it was used successfully to detect KAN in different aqueous solution, such as in spiked milk, the proposed sensor could be applied for specifically, sensitively and rapid determination for antibiotic contamination in food, water and biological samples. (C) 2020 Elsevier B.V. All rights reserved.
