摘要：
Ochratoxin A (OTA), a fungal toxin, induces various toxic effects in animals and humans through the enrichment of toxin residues. In this work, a dual-modal biosensor based on gold nanoparticles (AuNPs) and branched hybridization chain reaction (bHCR) was proposed for the detection of OTA. The strategy is contingent on OTA aptamer-bridged occurrence of bHCR and the salt-induced aggregation of AuNPs. OTA-apt/cDNA could be used to specifically identify the OTA and trigger bHCR reactions, producing a long-branched dsDNA polymer. The electroactive molecule-methylene blue (MB) can be inserted into the superstructure of branched DNA due to the formation of DNA polymers, leading to dynamic changes in MB redox signaling. The residual DNA hairpins were added and adhered to the surface of AuNPs, but they were inadequate to prevent the AuNPs from salt-induced aggregation. The dual-modal yields limits of detection of 4.8 pM (electrochemical assay) and 0.25 nM (colorimetric assay), respectively. It exhibited excellent specificity against common mycotoxins (AFB1, DON, FB1, ZEN), with satisfactory recoveries in corn flour (92.9–108.3 %). This aptasensor, which adopts a dual-modal strategy, features self-calibration to reduce false-positive results and improve accuracy. It demonstrates significant advantages in mycotoxin detection.
Ochratoxin A (OTA), a fungal toxin, induces various toxic effects in animals and humans through the enrichment of toxin residues. In this work, a dual-modal biosensor based on gold nanoparticles (AuNPs) and branched hybridization chain reaction (bHCR) was proposed for the detection of OTA. The strategy is contingent on OTA aptamer-bridged occurrence of bHCR and the salt-induced aggregation of AuNPs. OTA-apt/cDNA could be used to specifically identify the OTA and trigger bHCR reactions, producing a long-branched dsDNA polymer. The electroactive molecule-methylene blue (MB) can be inserted into the superstructure of branched DNA due to the formation of DNA polymers, leading to dynamic changes in MB redox signaling. The residual DNA hairpins were added and adhered to the surface of AuNPs, but they were inadequate to prevent the AuNPs from salt-induced aggregation. The dual-modal yields limits of detection of 4.8 pM (electrochemical assay) and 0.25 nM (colorimetric assay), respectively. It exhibited excellent specificity against common mycotoxins (AFB1, DON, FB1, ZEN), with satisfactory recoveries in corn flour (92.9–108.3 %). This aptasensor, which adopts a dual-modal strategy, features self-calibration to reduce false-positive results and improve accuracy. It demonstrates significant advantages in mycotoxin detection.
摘要：
In this work, a dual-signal ratiometric electrochemical aptasensor based on the hybrid chain reaction (HCR) and streptavidin-modified magnetic beads (SA-MBs) was developed to rapidly detect zearalenone (ZEN). The HCR, as a powerful signal amplification technique to imporve the signal of sonser. When the target is present, they specifically bind with ZEN-Apt and release ZEN-cDNA to trigger HCR. Simultaneously, more double-stranded DNA causes the signal of Thi to be blocked. As a result, the two signals tend to change in the opposite direction as the ZEN concentration changes. Additionally, the peak current ratio of I Thi /I Fc showed a positive correlation with the ZEN concentration. Under optimal conditions, the constructed biosensor showed an excellent linear detection range (1.0 × 10 −10 mol/L to 1.0 × 10 −6 mol/L), a low detection limit (4.4 × 10 −11 mol/L) and high specificity for ZEN. In addition, the detection method retains the characteristics of low cost and rapid detection of electrochemical detection, while improving the detection limit and detection accuracy via SA-MBs and internal reference signal. This provides a new idea for the practical detection of ZEN.
In this work, a dual-signal ratiometric electrochemical aptasensor based on the hybrid chain reaction (HCR) and streptavidin-modified magnetic beads (SA-MBs) was developed to rapidly detect zearalenone (ZEN). The HCR, as a powerful signal amplification technique to imporve the signal of sonser. When the target is present, they specifically bind with ZEN-Apt and release ZEN-cDNA to trigger HCR. Simultaneously, more double-stranded DNA causes the signal of Thi to be blocked. As a result, the two signals tend to change in the opposite direction as the ZEN concentration changes. Additionally, the peak current ratio of I Thi /I Fc showed a positive correlation with the ZEN concentration. Under optimal conditions, the constructed biosensor showed an excellent linear detection range (1.0 × 10 −10 mol/L to 1.0 × 10 −6 mol/L), a low detection limit (4.4 × 10 −11 mol/L) and high specificity for ZEN. In addition, the detection method retains the characteristics of low cost and rapid detection of electrochemical detection, while improving the detection limit and detection accuracy via SA-MBs and internal reference signal. This provides a new idea for the practical detection of ZEN.
摘要：
Anelectrochemical aptasensor was used for the fast and sensitive detection of zearalenone (ZEN) based on the combination of Co(3)O(4)/MoS(2)/Au nanocomposites and the hybrid chain reaction (HCR). The glassy carbon electrode was coated with Co(3)O(4)/MoS(2)/Au nanomaterials to immobilize the ZEN-cDNA that had been bound with ZEN-Apt by the principle of base complementary pairing. In the absence of ZEN, the HCR could not be triggered because the ZEN-cDNA could not be exposed. After ZEN was added to the surface of the electrode, a complex structure was produced on the modified electrode by the combination of ZEN and ZEN-Apt. Therefore, the ZEN-cDNA can raise the HCR to produce the long-strand dsDNA structure. Due to the formation of dsDNA, the methylene blue (MB) could be inserted into the superstructure of branched DNA and the peak currents of the MB redox signal dramatically increased. So the concentration of ZEN could be detected by the change of signal intensity. Under optimized conditions, the developed electrochemical biosensing strategy showed an outstanding linear detection range of 1.0×10(-10) mol/L to 1.0×10(-6) mol/L, a low detection limit (LOD) of 8.5×10(-11) mol/L with desirable selectivity and stability. Therefore, the fabricated platform possessed a great application potential in fields of food safety, medical detection, and drug analysis.
摘要：
BACKGROUND Diabetes is a metabolic disease characterized by hyperglycemia, which has increased the global medical burden and is also the main cause of death in most countries. AIM To understand the knowledge structure of global development status, research focus, and future trend of the relationship between diabetes and metabolomics in the past 20 years. METHODS The articles about the relationship between diabetes and metabolomics in the Web of Science Core Collection were retrieved from 2002 to October 23, 2023, and the relevant information was analyzed using CiteSpace6.2.2R (CiteSpace), VOSviewer6.1.18 (VOSviewer), and Bibliometrix software under R language. RESULTS A total of 3123 publications were included from 2002 to 2022. In the past two decades, the number of publications and citations in this field has continued to increase. The United States, China, Germany, the United Kingdom, and other relevant funds, institutions, and authors have significantly contributed to this field. Scientific Reports and PLoS One are the journals with the most publications and the most citations. Through keyword co-occurrence and cluster analysis, the closely related keywords are "insulin resistance", "risk", "obesity", "oxidative stress", "metabolomics", "metabolites" and "biomarkers". Keyword clustering included cardiovascular disease, gut microbiota, metabonomics, diabetic nephropathy, molecular docking, gestational diabetes mellitus, oxidative stress, and insulin resistance. Burst detection analysis of keyword depicted that "Gene", "microbiota", "validation", "kidney disease", "antioxidant activity", "untargeted metabolomics", "management", and "accumulation" are knowledge frontiers in recent years. CONCLUSION The relationship between metabolomics and diabetes is receiving extensive attention. Diabetic nephropathy, diabetic cardiovascular disease, and kidney disease are key diseases for future research in this field. Gut microbiota, molecular docking, and untargeted metabolomics are key research directions in the future. Antioxidant activity, gene, validation, mass spectrometry, management, and accumulation are at the forefront of knowledge frontiers in this field.
期刊：
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2023年13:1257817 ISSN：2235-2988
通讯作者：
Song, HP;Cai, X
作者机构：
[Song, Houpan; Sun, Qifang; Zeng, Meiyan] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Song, Houpan; Yuan, Chengzhi; Sun, Qifang] Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med TCM Diagnost, Changsha, Hunan, Peoples R China.;[Yuan, Chengzhi; Lu, Jing] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Zhou, Sainan] First Hosp Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Cai, Xiong; Cai, X] Hunan Univ Chinese Med, Sch Int Educ, Changsha, Hunan, Peoples R China.
通讯机构：
[Song, HP ; Cai, X ] H;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med TCM Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Int Educ, Changsha, Hunan, Peoples R China.
摘要：
Helicobacter pylori, a gram-negative microaerophilic pathogen, causes several upper gastrointestinal diseases, such as chronic gastritis, peptic ulcer disease, and gastric cancer. For the diseases listed above, H. pylori has different pathogenic mechanisms, including colonization and virulence factor expression. It is essential to make accurate diagnoses and provide patients with effective treatment to achieve positive clinical outcomes. Detection of H. pylori can be accomplished invasively and noninvasively, with both having advantages and limitations. To enhance therapeutic outcomes, novel therapeutic regimens, as well as adjunctive therapies with probiotics and traditional Chinese medicine, have been attempted along with traditional empiric treatments, such as triple and bismuth quadruple therapies. An H. pylori infection, however, is difficult to eradicate during treatment owing to bacterial resistance, and there is no commonly available preventive vaccine. The purpose of this review is to provide an overview of our understanding of H. pylori infections and to highlight current treatment and diagnostic options.
作者机构：
[Liao, Zhibing; Yao, Liu; Liu, Yan; Wu, Yaohui; Wang, Yonghong] Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha;410004, China;[Ning, Ge] International Education Institute, Hunan University of Chinese Medicine, Changsha;410208, China;[Liao, Zhibing; Yao, Liu; Liu, Yan; Wu, Yaohui; Wang, Yonghong] 410004, China
通讯机构：
[Yonghong Wang] H;[Ge Ning] I;Hunan Provincial Key Laboratory for Forestry Biotechnology & International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China<&wdkj&>International Education Institute, Hunan University of Chinese Medicine, 410208, Changsha, China
