Biosensors and Bioelectronics,2019年124-125:82-88 ISSN：0956-5663
Wang, Yonghong;Liu, Gaoqiang
[GaoqiangLiu] Hunan Provincial Key Laboratory for Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China. Electronic address: email@example.com;[XiaoxiaoHe; KeminWang] State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, China;[YaohuiWu; BaiquanZeng; ShunWu] Hunan Provincial Key Laboratory for Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China;[GeNing] International Education Institute, Hunan University of Chinese Medicine, 410208, Changsha, China;[YonghongWang] Hunan Provincial Key Laboratory for Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China. Electronic address: firstname.lastname@example.org
[Liu, Gaoqiang; Wang, Yonghong] Hunan Provincial Key Laboratory for Forestry Biotechnology, College of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China. Electronic address:
Smartly coupling exonuclease-induced target recycling signal amplifications with beta-cyclodextrin host-guest recognition, a novel "signal-on" aptamer sensor for sensitive determination of ochratoxin A (OTA) was proposed for the first time. Firstly, the formation of double-strand DNA (dsDNA) was occurred by hybridizing OTA aptamer with its complementary DNA (cDNA) and as the probe DNA the cDNA at its 3' terminal was labeled with methylene blue (MB). Next, when OTA was present, the aptamer tended to form aptamer-OTA complex with conformation of G-quadruplex instead of aptamer-cDNA duplex, leading to thus the probe DNA separating from dsDNA complex. Then the RecJf exonuclease was added, demolishing partially G-quadruplex structure and releasing a certain number of OTA. Sequentially, those released OTA would continue to react with the rest of aptamer in dsDNA, drawn into development of a new round of G-quadruplex complex, where the target cycling was realized. Meanwhile, as a signal molecule, MB modified on cDNA was liberated along with the cDNA being digested into monoucleotides by RecJf exonuclease, capable of diffusing onto the electrode surface due to host-guest recognition with beta-cyclodextrin, whereupon the signal was enriched and yielded. In this way, cycles of target with continuous output of signal indicators were undergone, in which the detection of target was in return fulfilled with signal amplification owing to the joint endeavor of exonuclease and beta-cyclodextrin. Under the optimal conditions, the raising signal maintained a linear relation with the logarithm of the target concentrations ranging from 10pg/mL to 10.0ng/mL and the detection limit reached as low as 3pg/mL. This brand-new strategy was simple and low-cost but satisfactory in terms of detection limit, range and sensitivity, in all possibility to be applied extensively for diverse targets detection by easily alternating the corresponding aptamers.
Ali Sheikh, Md Sayed;Salma, Umme;Zhang, Baohai;Chen, Jimei;Zhuang, Jian;Ping, Zhu
Oxidative Medicine and Cellular Longevity,2016年2016:5893064 ISSN：1942-0900
[Zhang, Baohai] Department of Cardiology, Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, China;[Salma, Umme] Department of Gynecology and Obstetrics, Xiangya 3rd Hospital, Central South University, Changsha, Hunan, 410013, China;[Chen, Jimei; Ping, Zhu; Ali Sheikh, Md Sayed; Zhuang, Jian] Department of Cardiovascular Surgery, Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangzhou, 510100, China;[Ali Sheikh, Md Sayed] Department of Cardiology, Hunan Traditional Chinese Medical University, International Education Institute, Changsha, Hunan, 410208, China
[Ping, Zhu] Guangdong Acad Med Sci, Guangdong Gen Hosp, Guangdong Cardiovasc Inst, Dept Cardiovasc Surg, Guangzhou 510100, Guangdong, Peoples R China.
Aging population - Brain natriuretic peptide - Clinical management - Effective management - International community - Novel diagnostics - Risk stratification - Therapeutic values
A novel aptamer sensor for ratiometric ochratoxin A (OTA) detection was constructed based on the host-guest competition mode. Firstly, the aptamer modified with methylene blue (MB) at its terminal was fixed on the surface of the gold electrode via the host-guest recognition with beta-cyclodextrin (beta-CD). Then the MoS2 nanosheets/Au NPs nanocomposites were used for the signal amplification. When OTA was present, the configuration of MB-aptamer transformed from vertical linear to G-quadruplex, thus making itself unable to get close to the electrode surface and causing a decrease in the redox current of the MB-aptamer. Whereas the free ferrocenecarboxylic acid could recognize and occupy the vacant site and produce signals in the current, resulting in the "ratiometric" effect. Consequently, on the basis of the "dual-signal" principle, a highly sensitive detection of OTA was made, indicating that the OTA concentration had a linear response to the signal of sensor between 0.1 nM and 50 nM, and as low as 0.06 nM OTA had been detected. Overall, this method for OTA detection was constructed easily with high sensitivity and specificity, providing a significant platform for highly efficient detection of OTA in biological samples. (C) 2018 Elsevier Ltd. All rights reserved.
[陈明] Department of Stomatology, the First Affiliated Hospital of Hunan University of CM, Changsha 410007;[章薇; 娄必丹; 潘江] Department of Acupuncture and Moxibustion;[林碧莲] College of International Education, Hunan University of CM;[潘江] Hunan University of CM, Changsha 410007, China
diabetic retinopathy;combined therapy of traditional Chinese m edicine w ith laser photocoagulation;research progress
Diabetic retinopathy（ DR） is the most severe complication of diabetic eye disease, which can eventually lead to irreversible blindness,thus seriously impacting on patients＇ quality of life. At present,surgical operation has been widely carried out on the treatment of DR in China. For the pr proliferative DR（ PDR） and PDR,panretinal photocoagulation has often been adopted. By damaging the retina in ischemicarea so as to reduce oxygen demand,the neovascularization can be prevented and the patient＇s condition can be stopped from getting worse. Traditional Chinese m edicine（ TCM） has an abundant and personalized therapeutic regimen for the treatment of DR. This paper reviews the research progress on combined therapy of TCM with laser photocoagulation for treatment of DR.