作者机构：
[Yao, Zhen; Jiang, Pengfei; Ou, Chen; Peng, Qinghua; Yang, Yijing; Tian, Ye; Song, Houpan] Hunan Univ Chinese Med, Hunan Prov Key Lab Prevent & Treatment Ophthalmol, Changsha 410208, Hunan, Peoples R China.;[Peng, Jun; Peng, Qinghua] Hunan Univ Chinese Med, Dept Ophthalmol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Jiang, Pengfei; Ou, Chen; Peng, Qinghua; Zeng, Meiyan; Song, Houpan] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Res Chinese Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Jiang, Pengfei; Peng, Qinghua; Ou, Chen; Zeng, Meiyan; Yang, Yijing; Yao, Zhen; Song, Houpan; Tian, Ye] H;[Peng, Jun] D;Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China. Electronic address:;Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China. Electronic address:;Department of Ophthalmology, The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, China. Electronic address:
摘要：
ETHNOPHARMACOLOGICAL RELEVANCE: Fructus Lycii and Salvia miltiorrhiza Bunge (FS) are popular Chinese herbs for the treatment of retinitis pigmentosa (RP). AIM OF THE STUDY: This study was to evaluate protective effects of FS extract on RP and to explore whether FS extract exerts its protective effects via oxidative stress by regulating Nrf2/HO-1 signaling pathway. MATERIAL AND METHODS: FS extract were identified by UPLC chromatographic analysis. Rd10 mice as the model of RP, followed by a 4-week FS extract treatment by intragastric administration. After the animal sacrifice, histopathological examination and Scotopic electroretinography (ERG) analysis were assessed. The oxidative stress markers were determined and the expression levels of Nrf2 and HO-1 mRNA were evaluated by qRT-PCR. The expression and distribution of Nrf2 and HO-1 protein were determined by Western blot and immunohistochemistry. RESULTS: The morphological changes of Outer nuclear layer (ONL) thickness and number of the ONL were observed with a significant increased, and the functional changes of a-amplitude and b-wave amplitude were measured with a markedly increased. Treatment with FS extract remarkably increased levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and decreased level of malondialdehyde (MDA). Moreover, FS extract up-regulated mRNA and protein expression of Nrf2 and HO-1. CONCLUSIONS: This study indicated that FS extract can improve retinal morphology and function, which may have occurred through the regulation of the Nrf2/HO-1 pathway to inhibit the oxidative reaction.
通讯机构：
[Peng, Qinghua] H;Hunan Univ Chinese Med, Changsha 410208, Hunan, Peoples R China.
摘要：
Background. Qing Guang An Granule (QGAG), a Chinese patent medicine, has been used clinically to treat glaucoma for more than 20 years. Objective. To explore the possible mechanism of treatment of QGAG in glaucoma by using network pharmacology and molecular docking in this study. Methods. Active compounds and targets of each herb in QGAG were retrieved via the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). Glaucoma-related targets were acquired from OMIM and DisGeNET database. Key targets of QGAG against glaucoma were acquired by overlapping the above targets via the Venn diagram. Using the DAVID, Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the key targets were performed. The docking process was performed using the AutoDock 4.2.6 and AutoDock Vina 1.1.2. Results. The 55 active compounds and 173 targets were obtained and constructed a compound-target network. The 20 key targets of QGAG in treating glaucoma were acquired, and these targets are involved in the apoptotic process, cellular response to hypoxia, negative regulation of cell growth, and ovarian follicle development. The main pathways are p53, HIF-1, PI3K-Akt, and neurotrophin signaling pathway. Conclusion. QGAG may exert a protective effect by acting on the optic nerve at a molecular and systemic level. This study can provide a certain basis for future researches on exploring the QGAG in treating glaucoma and provide new ideas for developing new drugs.
作者机构：
[彭清华] Institute of Tradition Chinese Medicine Diagnostics, Changsha, 410208, China;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[喻嵘; 宋厚盼; 蔡雄; Zeng M.-Y.; 陈小娟; 谢明霞; 陈新怡] Institute of Tradition Chinese Medicine Diagnostics, Changsha, 410208, China, College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
作者机构：
[蔡雄] Institute of Innovation and Applied Research in Chinese Medicine, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[陈聪; 李鑫; 彭清华; 宋厚盼] School of Basic Chinese Medical Sciences, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[王炜] School of Pharmaceutical Sciences, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[唐琳] School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangdong, Guangzhou, 510224, China;[刘良] State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Macau, 999078, Macau
通讯机构：
[CAI, X.] I;Institute of Innovation and Applied Research in Chinese Medicine, Hunan, China
关键词：
青附蠲痹汤;类风湿关节炎;制备工艺;质量控制;安全性;抗炎镇痛
摘要：
Objective Qing Fu Juan Bi Tang (QFJBT) is an anti-arthritic Chinese medicine formula consisting of five herbs: Aconiti Lateralis Radix Praeparata (Fu Zi, 附子), Sinomenii Caulis (Qing Feng Teng, 青风藤), Astragali Radix (Huang Qi, 黄芪), Paeoniae Radix Alba (Bai Shao, 白芍) and Moutan Cortex (Mu Dan Pi, 牡丹皮), which have well-established histories of use for treatment of rheumatic and arthritic diseases. We intended to establish the optimized and standardized pharmaceutical procedures and manufacturing processes for the pilot production of QFJBT to develop it as a novel botanical drug product for treatment of rheumatoid arthritis (RA). Methods The combinative approaches of chemical assessment, toxicological and pharmacological evaluation were explored to define the pharmaceutical preparation of QFJBT. Results The optimized and standardized pharmaceutical procedures and manufacturing processes for the pilot production of QFJBT were established in terms of greatest chemical contents of bioactive constituents, potent anti-inflammatory and antinociceptive activities, and favorable safety profile. Quality analysis of the pilot product of QFJBT by high-performance liquid chromatography (HPLC) demonstrated that the chromatographic fingerprint profiles of three batches of QFJBT were basically identical and the contents of four characteristic and bioactive markers were relatively consistent. General toxicological studies showed a favorable safety profile of QFJBT. The maximum tolerated single dose of QFJBT was determined in both sexes of rats to be 33.63 g/kg body weight which is equivalent to 346 times of clinical dose. In the chronic oral toxicity study, the results of laboratory investigation showed that QFJBT at doses of 3.89, 6.80 and 9.72 g/kg body weight (equivalent to 40, 70 and 100-fold clinical doses, respectively) caused no changes in all hematological parameters and blood biochemical parameters of rats. No mortality or specific toxic responses were observed in animals after three months of repeated dosing with QFJBT. Conclusion The optimized and standardized pharmaceutical and manufacturing processes for the production of QFJBT have been successfully screened and identified through established rigorous in-process controls.
