摘要：
Background To investigate the similarities and differences in cerebral responses to puncturing at different acupoints for treating meal-related functional dyspepsia (FD). Methods Twenty right-handed FD patients were enrolled and randomized divided into two groups. Each patient received 20 sessions' electroacupuncture treatment. The acupoints used in Group A were four acupoints on the Stomach Meridian, and the acupoints used in Group B were four acupoints on the Gallbladder Meridian. PET-CT scans were performed before and after acupuncture treatment to record the changes of cerebral glycometabolism. Key Results After treatment, the dyspepsia symptoms and the quality of life (QOL) of the patients in each group were significantly improved (p < 0.05) and there was insignificant difference in efficacy between the two groups (p > 0.05). In Group A, deactivation in brainstem, bilateral anterior cingulate cortex (ACC) and cerebellum, left superior medial frontal gyrus, orbital frontal cortex (OFC), and thalamus, etc., and activation in bilateral middle cingulate cortex (MCC), precuneus and lingual gyrus, etc. were observed. In Group B, deactivation in brainstem, bilateral thalamus, putamen, ACC, postterior cingulate cortex, hippocampus and cerebellum, etc., and activation in bilateral MCC, precuneus, left OFC, etc. were observed (p < 0.05, Family-wise error corrected). Conclusions & Inferences Different acupoints have similar clinical efficacy but relatively different cerebral responses. The influence on the sensory transduction regions (brainstem and thalamus) and visceral modulation regions might be the common mechanism of different acupoints treating for FD, and the modulation on some emotion/cognition-related areas (e.g., prefrontal cortex) is the potential difference between the different acupoints.
摘要：
Ethnopharmacological relevance: Ginseng is the dried root of Panax ginseng C.A. Mayer. Since ancient times, ginseng has been used as one kind of treatment drug or tonic in China and even other eastern countries like Korea and Japan. Pharmacological active chemical ingredients and its extract of ginseng are a mixture of triterpenoid saponins, collectively called ginsenosides. Among them, ginsenoside Rg1 is the most pharmacological active one. Aim of the study: Based on prior experimental results and the understanding of alcoholic hepatitis, the major aim of this study is to investigate whether Rg1 is beneficial in a rodent model mimic alcoholic hepatic injury associated with binge drinking and explore the underlying possible mechanisms. Materials and methods: C57BL/6 mice were given oral consumption of 6 g/kg alcohol 1 h after treated with Rg1 (10, 20 and 40 mg/kg) or dexamethasone (1 mg/kg) for 9 consecutive days. Biochemical analyses were performed and liver fragments were processed for microscopy, immunohistochemistry and western blot analysis. Results: According to our data, Rg1 treatment significantly reversed the high mortality rate induced by alcohol consumption and also alleviated liver impairment as evidenced by the decrease of serum parameters. Meanwhile, histological and ultrastructural analysis of alcoholic groups showed hepatocellular impairment but restored in Rg1-treated groups. Overproductive inflammatory cytokines were also suppressed by Rg1 in alcohol-intoxicated mouse livers. In addition, changes of GR related NF-kappa B pathway, including phospho-I kappa B-alpha, were also modulated to normal levels. Conclusion: This study demonstrates that Rg1 might promote GR mediating the repression of NF-kappa B and inhibit the inflammatory reactions in alcoholic hepatitis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
摘要：
目的：探讨益心泰颗粒对慢性心力衰竭（ CHF）兔心肌重构的影响。方法用阿霉素建立CHF家兔模型，将造模成功的家兔分为模型组、益心泰低、中、高剂量组和氯沙坦钾组；另设正常对照组。灌胃4周后观察超声心动图指标、心肌重构指标。结果与模型组比较，各用药组左心室后壁（LVPW）厚度、左心室收缩末期内径（LVIDs）、左心室舒张末期内径（LVIDd）、心脏指数（CI）及左心室重量指数（ LVWI）明显降低，差异有统计学意义（ P ＜0．05， P ＜0．01）；与模型组比较，益心泰中、高剂量组室间隔厚度（IVS）显著降低，差异有统计学意义（ P ＜0．01）。与益心泰低剂量组比较，益心泰中、高剂量组IVS、LVPW、CI、LVWI降低，差异有统计学意义（ P ＜0．05， P ＜0．01）；益心泰高剂量组LVIDs及LVIDd均明显降低，差异有统计学意义（ P ＜0．05）。结论益心泰颗粒能改善CHF兔心肌重构，且益心泰中、高剂量组疗效明显优于低剂量组。
摘要：
Objective： To explore the impact of Yixintai （益心泰） on Ang Ⅱ, ALD and BNP in serum of rabbit with chronic heart failure（CHF）. Methods： The rabbit model of CHF was established with adriamycin. These successful CHF models of rabbits were divided into model group, high, middle and low dose of Yixintai group and Furosemide group, plus the normal control group. The levels of Ang Ⅱ, ALD and BNP in serum were measured after 4 weeks of medication. Results： Compared with the model group, the levels of AngⅡ, ALD and BNP in serum were significantly lower（P〈0.01）, and the middleand high groups were both superior to the low dose group. Conclusion： Yixintai can reduce the levels of Ang Ⅱ, ALD and BNP in serum of patients with CHF, and the middleand high groups were both superior to the low dose group.
期刊：
BMC Complementary Medicine and Therapies,2015年15(1):1-11 ISSN：2662-7671
通讯作者：
Liu, Xinmin
作者机构：
[Lu, Cong; Wang, Qiong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing 100193, Peoples R China.;[Lu, Cong; Wang, Qiong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Peking Union Med Coll, Beijing 100193, Peoples R China.;[Chen, Lingling; Wang, Qiong; Liao, Duanfang; Bu, Lanlan; Shi, Zhe; Liu, Xinmin] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Changsha 410208, Hunan, Peoples R China.;[Chen, Shanguang; Li, Yinghui; Wang, Qiong; Qu, Lina] China Astronaut Res & Training Ctr, Beijing 100094, Peoples R China.;[Liu, Xinmin] Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Malianwa North Rd 151, Beijing 100193, Peoples R China.
通讯机构：
[Liu, Xinmin] C;Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Malianwa North Rd 151, Beijing 100193, Peoples R China.
关键词：
A beta(1-40);Cognitive impairment;Tong Luo Jiu Nao;Reward-directed instrumental learning;ERK/CaMKII/CREB signaling
摘要：
Background: Tong Luo Jiu Nao (TLJN), a modern formula of Chinese medicine extracts on the basis of Traditional Chinese Medicine theory, has been used to treat dementia. The present study aimed to investigate its ameliorating effects on A beta(1-40)-induced cognitive impairment in rats using a series of novel reward-directed instrumental learning (RDIL) tasks, and to determine its possible mechanism of action. Methods: Rats were pretreated with TLJN extract (0.9 and 1.8 g/kg, p.o.) for 10 daysbefore surgery, and were trained to gain reward reinforcement by lever pressing at the meantime. Thereafter, rats received a bilateral microinjection of A beta(1-40) in CA1 regions of the hippocampus. Cognitive performance was evaluated with the goal directed (higher response ratio) and habit (visual signal discrimination and extinction) learning tasks, as well as on the levels of biochemical parameters and molecules. Results: Our findings first demonstrated that TLJN can improve A beta(1-40)-induced amnesia in RDIL via enhancing the comprehension of action-outcome association and the utilization of cue information to guide behavior. Then, its ameliorating effects should attribute to the modulation of ERK/CaMKII/CREB signaling in the hippocampus. Conclusion: TLJN can markedly enhance cognitions of A beta(1-40) microinjection animal model in adaptive behavioral tasks. It has the potential, possibly as complementary and alternative therapy, to prevent and/or delay the deterioration of cognitive impairment in AD.
