作者机构：
[谢谦; 李顺祥; 江星明; 廖端芳; 王炜; Abass Ali; Kevin K.Schrader; Stephen O.Duke; Charles L.Cantrell; David E.Wedge] 湖南中医药大学药学院;[谢谦; 李顺祥; 江星明; 廖端芳; 王炜; Abass Ali; Kevin K.Schrader; Stephen O.Duke; Charles L.Cantrell; David E.Wedge] National Center for Natural Products Research,University of Mississippi;[谢谦; 李顺祥; 江星明; 廖端芳; 王炜; Abass Ali; Kevin K.Schrader; Stephen O.Duke; Charles L.Cantrell; David E.Wedge] USDA-ARS-NPURU,University of Mississippi
作者机构：
1. College of Chemistry and Chemical Engineering,Hunan Institute of Science and Technology;2. College of Pharmacy,Hunan University of Chinese Medicine
摘要：
[Objective]To select the optimal extraction technology of total saponins from RADIX ROSAE LAEVIGATAE;and to purify the total saponins by macroporous adsorption resins.[Methods]With the content of oleanolic acid as the reference substance,the total saponins content in RADIX ROSAE LAEVIGATAE was detected by UV-Vis spectrophotometry.Single factor test was adopted.The effects of extraction time,solid-liquid ratio and ethanol concentration on the total saponins of RADIX ROSAE LAEVIGATAE were investigated.Orthogonal test was designed according to the test results of single factor test.Purification effects of different types of macroporous resins on the total saponins of RADIX ROSAE LAEVIGATAE were evaluated according to the properties of static and dynamic adsorption and desorption properties.[Results]The influencing degrees of factors affecting the extraction rate of total saponins were in the order of solid-liquid ratio>reflux time>ethanol concentration.The optimal extraction technology was 1 h reflux time,1∶10 solid-liquid ratio,70%ethanol concentration,and two extraction times with 1 h each time.NKA-9 macroporous resin had the optimal purification effects on the total saponins of RADIX ROSAE LAEVIGATAE,and the purity of total saponins enhanced from 36.8%to 62.9%,and the elution rate reached 87.8%.[Conclusions]This method was effective,simple and feasible.
作者机构：
[Yu PAN; Shun-Xiang LI; Dan HUANG; Min-Qiu PAN] School of Pharmacy,Hunan University of Chinese Medicine;[Yu PAN; Shun-Xiang LI; Dan HUANG; Min-Qiu PAN] Hunan Hospitals of Traditional Chinese and Western Medicine;[Yu PAN; Shun-Xiang LI; Dan HUANG; Min-Qiu PAN] Liver Cancer Research Institute,Hunan Academy of Traditional Chinese Medicine
会议名称：
2013年代谢组学与中医药现代研究学术论坛
会议时间：
2013-10-14
会议地点：
太原
会议论文集名称：
2013年代谢组学与中医药现代研究学术论坛论文集
关键词：
Ganfule formula;Chinese herbal medicine;hepatocellular carcinoma;network pharmacology;drug discovery and combination
摘要：
Ethmopharmcology relevance: Ganfule(GFL),one of herbal medicines formula in china, has been widely used as the prevention of hepatic cirrhosis, hepatitical viral and liver cancer agent.However, its material basis that account for therapeutic effects and mechanisms of action at molecular-level remain unclear.Methods: Here, we constructed a computational systems approach that integrates druglikeness prescreening, ADME (absorption, distribution, metabolism, and excretion) prediction, molecular docking and multi-components, multi-targets, multi-pathways networks construction.Results: The network-based approach indentified 69 potential active ingredients that are closely associated 8 targets relating to Hepatocellular carcinoma (HCC); Herba Artemisiae(Yinchenhao) and Radix Bupleuri(Chaihu) represent the principal components for the prevention of HCC;P13K-Akt signaling pathway, mTOR signaling pathway and Jak-STAT signaling pathway are major important mechanisms of treating HCC;GFL formula may have effects of a series of diseases of cancer, infection and nervous system, etc.Conculsion: This work successful illustrates the potential active components and the mechanism of efficiency of GFL formula against HCC.Therefore, such a network pharmacology strategy is expected to elucidating the material basis and mechanisms of action of Chinese herbal medicine formula.
通讯机构：
[Li, Shun-xiang] H;Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
摘要：
This study aimed to investigate the composition of three major stilbenes (mulberroside A, oxyresveratrol, and resveratrol) in different portions of mulberries collected in different seasons and their change molds during growth by high-performance liquid chromatography. Mulberroside A levels were the highest in the bark and roots of Morus atropurpurea Roxb, Morus alba Linn, and Morus latifolia Poir. Oxyresveratrol levels were the highest in roots and stem. Both of these high levels were in September. The amount of resveratrol was very low in all samples. In the stem, Morus latifolia Poir contained more mulberroside A than the other two mulberries. Mulberroside A was not detected in the leaves of the three mulberries. In Morus atropurpurea Roxb seedlings, the root tended to contain more of the three stilbenes than leaves. The temporal peaks of resveratrol were always ahead of those for oxyresveratrol. The levels of the stilbenes varied in different portions of the varieties of mulberries collected in different season and in the seedlings of Morus atropurpurea Roxb.
作者机构：
[严建业; 徐菲; 彭买娇; 李顺祥; 罗堃] Key Laboratory of Modernization of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China;[王元清] Key Laboratory of Modernization of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China, Laboratory of Biotechnology and Engineering, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China
通讯机构：
[Yan, J.-Y.] K;Key Laboratory of Modernization of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China
作者机构：
[周晋] School of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;[李顺祥; 季梅; 黄丹; 虢小翊] School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;Key Lab. of Chinese Materia Medica Research and Development, Hunan Academy of Chinese Medicine, Changsha 410013, China;[颜新培] Sercultural Sciences Institute of Hunan Province, Changsha 410127, China;[郑群怡] Natsource Chemicals (Changsha) Corporation, Changsha 410329, China
通讯机构：
School of Bioscience and Technology, Hunan Agricultural University, China
