摘要：
Ralstonia solanacearum, the causal agent of bacterial wilt, is a devastating plant pathogenic bacterium that infects more than 450 plant species. Until now, there has been no efficient control strategy against bacterial wilt. In this study, we screened a library of 100 plant-derived compounds for their antibacterial activity against R. solanacearum. Twelve compounds, including harmine, harmine hydrochloride, citral, vanillin, and vincamine, suppressed bacterial growth of R. solanacearum in liquid medium with an inhibition rate higher than 50%. Further focus on harmine revealed that the minimum inhibitory concentration of this compound is 120 mg/L. Treatment with 120 mg/L of harmine for 1 and 2 h killed more than 90% of bacteria. Harmine treatment suppressed the expression of the virulence-associated gene xpsR. Harmine also significantly inhibited biofilm formation by R. solanacearum at concentrations ranging from 20 mg/L to 60 mg/L. Furthermore, application of harmine effectively reduced bacterial wilt disease development in both tobacco and tomato plants. Collectively, our results demonstrate the great potential of plant-derived compounds as antibacterial agents against R. solanacearum, providing alternative ways for the efficient control of bacterial wilt.
作者：
Li B.;Peng C.-Y.;Chen Y.-Y.;Duan S.-L.;Peng Y.-L.;...
期刊：
中草药,2021年52(02):335-340 ISSN：0253-2670
通讯作者：
Li, S.-X.;Wang, W.
作者机构：
[Danial M.; Li B.; Peng C.-Y.; Duan S.-L.; Wang W.; Peng Y.-L.] TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[Chen Y.-Y.; Li S.-X.] Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Wang, W.] T;[Li, S.-X.] H;Hunan Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, China;TCM and Ethnomedicine Innovation & Development International Laboratory, China
作者机构：
[Sun, Zhiya; Zhang, Yi; Yang, Huaming; Long, Mei] Cent South Univ, Ctr Mineral Mat, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.;[Sun, Zhiya; Zhang, Yi; Yang, Huaming; Long, Mei] Cent South Univ, Hunan Int Joint Lab Mineral Mat, Changsha 410083, Peoples R China.;[Liu, Xinxuan] Hunan Univ Chinese Med, Sci & Technol Innovat Ctr, Changsha 410208, Peoples R China.;[Zhang, Yi; Yang, Huaming] Cent South Univ, Key Lab Clay Mineral Funct Mat China Bldg Mat Ind, Changsha 410083, Peoples R China.;[Li, Shunxiang] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Yang, Huaming] C;Cent South Univ, Ctr Mineral Mat, Sch Minerals Proc & Bioengn, Changsha 410083, Peoples R China.
关键词：
Natural minerals;Ulcerative colitis;Therapy;In vivo evaluation;Drug
摘要：
Ulcerative colitis (UC) affects millions of people worldwide and is currently incurable. As such, there is an urgent need for effective drugs that will promote UC remission. This study investigated the therapeutic effects of five typical natural minerals on UC induced by dextran sulfate sodium (DSS): kaolinite (Kaol), halloysite (Hal), diatomite (Dia), montmorillonite (Mt), and palygorskite (Pal). These minerals were characterized by lamellar, tubular, columnar porous, and fibrous morphologies, as well as centered size distribution and negative charge. In vivo experiments indicated that natural minerals had a remission effect on UC and altered gut flora, especially Kaol. These results suggest that natural minerals can serve as drugs, or drug carriers, to attain synergistic effects with other drugs for UC therapy.
作者机构：
湖南中医药大学药学院,湖南 长沙 410208,中国;湖南省中药活性物质筛选工程技术研究中心,湖南 长沙 410208,中国;[唐敏; 严建业; 任杰; 李顺祥] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China<&wdkj&>Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, Changsha, Hunan, 410208, China
通讯机构：
[Li Shun-Xiang] S;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China<&wdkj&>Hunan Province Engineering Research Center of Bioactive Substance Discovery of Chinese Medicine, Changsha, Hunan, 410208, China
摘要：
A new bis-indole alkaloid, named arundaline (1), a new phenylpropanoid, named arundalcohol (2), and four known alkaloids, N-acetyltryptamine (3), trans-N-(p-coumaroyl)serotonin (4), trans-N-feruloylserotonin (5), and tuberosine B (6), were isolated from 70% aqueous ethanol extracts of the rhizomes of Arundo donax L. Their structures were elucidated by spectroscopic analysis and comparison of the data with literature values. Compounds 3–6 were isolated from the genus Arundo for the first time.
