摘要：
Objective： To observe the effect of Naotaifang extracts （脑泰方提取物） on plasma fibrinolytic activity of focal cerebral infarction in rats, and investigate the mechanisms of Naotaifang extracts in treatment of focal cerebral infarction. Methods： Thirty-three rats were weighted and put in order, then they were randomly divided into normal control group （n = 7）, sham-operated group （n = 7）, model group （n=7）, Naofukang （脑复康） group （n = 6） and Naotaifang extracts group （n=6）. The normal control group, sham-operated group and model group were given normal saline infused into stomach. Naofukang group was given 0.28g·kg^-1· d^-1 of 2.8% Naofukang solution infused into stomach. Naotaifang extracts group was given 2.8 g·kg^-1· d^-1 of Naotaifang extract powder. Neural function defect score and cerebral pathophysiology were used to assess the success of the model establishment. The levels of tissue plasmlnogen activator （t -PA） and plasminogen activator inhibitor （PAI） were detected in all the groups. Results.. The activity of t PA in model group was lower than that in the normal control group, while PAI was higher. In Naotaifang extracts group, the activity of t - PA and the ratio of t - PA/PAI increased significantly, PAI recovered to the normal level. The effect of Naofukang was similar to that of Naotaifang extracts. Conclusion ： Naotaifang can influence plasma fibrinolytic activity in rats with focal cerebral infarction by improving vascular endothelial function and fibrinolytic activity.
作者机构：
[陈懿; 葛金文; 邓冰湘] Department of Physiology Hunan University of Traditional Chinese Medicine,Changsha,410007,China;[陈懿; 葛金文; 邓冰湘] Lab of Vascular Biology,Hunan University of Traditional Chinese Medicine,Changsha,410007,China;[陈懿; 葛金文; 邓冰湘] Department of Physiology,Hunan University of Traditional Chinese Medicine,Changsha,410007,China
通讯机构：
[Ge Jin-wen] D;Department of Science and Technology, Hunan University of Traditional Chinese Medicine, Changsha
摘要：
OBJECTIVE: A new rat model of cerebral infarction was developed to elucidate the contribution of vascular endothelial cell during focal cerebral infarction formation. METHODS: Forty-eight Sprague-Dawley (SD) rats were randomly divided into the model group, sham operation group, and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing, neurological deficit, plasma tissue-type plasminogen activator (tPA) activity, plasminogen activator inhibitor (PAI) activity, thromboxane B(2) (TXB(2)) content, and 6-keto-prostaglandin (6-keto-PGF(1alpha)) content. RESULTS: (1) The highest neurological score appeared at 6 h after operation, descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique, pathological changes in brains were observed. (3) Compared with control group and sham operation groups, there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P < 0.05), PAI activity decreased markedly in the model group at 24 h after injection of sodium laurate. (4) In plasma TXB(2) concentration reached the highest level compared at 6 h after injection of sodium laurate, but there were not obvious differences in plasma 6-keto-PGF(1alpha) concentration among all groups (P > 0.05). CONCLUSION: Focal cerebral infarction in rats could be induced by some sodium laurate, showing ischemic cerebrum necrosis, function disorder of vascular endothelium-platelet, fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development, preventing and curing by traditional Chinese medicine.
