作者机构：
[陈新宇; 刘起立; 姚昆鹏; 张道平] Hunan University of Chinese Medicine,,The First Affiliated Hospital of Hunan University of Chinese Medicine;[陈青扬; 蔡虎志] The First Affiliated Hospital of Hunan University of Chinese Medicine
关键词：
急性心肌梗死;中药;生物信息学
摘要：
采用Gene Expression Omnibus(GEO)数据集联合机器学习研究急性心肌梗死(acute myocardial infarction,AMI)的差异基因,并预测具有调控作用的潜在成分及中药。从GEO数据...展开更多 采用Gene Expression Omnibus(GEO)数据集联合机器学习研究急性心肌梗死(acute myocardial infarction,AMI)的差异基因,并预测具有调控作用的潜在成分及中药。从GEO数据库下载AMI的人类全基因组数据集(GSE66360和GSE61145),以GSE66360作为测试集,通过R语言的normalize Between Arrays包进行校正后,再调用limma包获取差异基因(DEGs),对DEGs作Gene Ontology(GO)、Kyoto Encyclopedia of Genes and Genomes(KEGG)、Disease Ontology(DO)富集分析;采用SVM及随机森林树法筛选特征基因,利用GSE61145数据集对得出的特征基因进行验证;通过CTD数据库找到AMI特征基因所对应的中药成分,利用Coremine数据库映射中药成分所对应的中药,并依据《中药大辞典》、《中华本草》、《中国药典》等对所得中药的频次、四气、五味、归经进行汇总。通过对GSE66360数据集进行分析,得到317个差异基因,其中306个上调,11个下调,GO和KEGG富集分析显示AMI的差异基因主要涉及中性粒细胞介导的炎症和免疫反应、脂代谢异常、脂质和动脉粥样硬化相关通路等,DO富集分析表明差异基因与动脉硬化性心血管疾病、肺部疾患等密切相关。通过SVM及随机森林树法得到6个特征基因:ZFP36、GADD45A、PELI1、METRNL、MMP9、CXCL16。CTD映射到成分97种,Coremine数据库映射到中药824味,汇总后发现治疗AMI的中药以甘、苦、温为主,多归于脾、胃、肝经。经汇总后,调控AMI的特征基因(ZFP36、GADD45A、PELI1、METRNL、MMP9、CXCL16)成分主要有苯并[a]芘,四氯二苯二氧芑,对乙酰氨基酚等,中药有茶树根、郁金、人参等,其性味为甘、苦、温,归经多为脾、胃、肝经。收起
作者机构：
[何业文; 李平; 罗慕晴; 颜路悠; 黎建宇; 张堃; 刘音其] Department of Radiology, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[向辉春; 李宏伟] Department of Radiology, Huaihua Hospital of Traditional Chinese Medicine, Huaihua, 418099, China
通讯机构：
[Zhang, K.] D;Department of Radiology, China
作者机构：
Hunan University of Chinese Medicine, Changsha, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, China;[潘江; 罗容; 汤伟; 叶勇] The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;[范升; 申韬] Hunan University of Chinese Medicine,Changsha 410208,China
通讯机构：
[Yong Ye] T;The First Hospital of Hunan University of Chinese Medicine, Changsha, China
关键词：
Tuina;Massage;Intervertebral Disc Displacement;Low Back Pain;Pain Measurement;Visual Analog Scale;Posterior Muscle Chain
摘要：
Objective: To observe the clinical efficacy of sinew-bone balancing manipulation in treating lumbar disc herniation (LDH) and offer clinical evidence to support the concept of paying equal attention to sinew and bone. Methods: Sixty LDH patients were randomized into an observation group and a control group, with 30 cases in each group. The observation group was treated with the sinew-bone balancing manipulation, and the control group received conventional Tuina (Chinese therapeutic massage) manipulation. The clinical efficacy and posterior muscle chain tone effect were compared between the two groups by observing the visual analog scale (VAS) score, Japanese Orthopaedic Association (JOA) score, and posture-associated indicators. Results: The total effective rate was 86.7％ in the observation group, higher than 76.7％ in the control group, but the between-group difference in efficacy was statistically insignificant (P>0.05). After treatment, the VAS and JOA scores, angle-dependent muscle tone indicator of the posterior muscle chain, and lumbar posture symmetry showed significant changes in both groups (P<0.05). The VAS and JOA scores, angle-dependent muscle tone indicator of the posterior muscle chain, and lumbar posture symmetry in the observation group were significantly different from those in the control group after treatment (P<0.05), but the between-group difference in the general posture symmetry was statistically insignificant (P>0.05). Conclusion: Both the sinew-bone balancing and conventional Tuina manipulations can reduce pain in LDH patients, improve lumbar function, and adjust the angle-dependent muscle tone coefficient of the posterior muscle chain and lumbar posture symmetry; except for the general posture symmetry of the posterior muscle chain, the sinew-bone balancing manipulation wins out over the conventional Tuina manipulation.
作者机构：
[邓泽成; 谢峥嵘] Hunan University of Chinese Medicine;[章薇; 王鑫; 柯超; 唐媛媛; 钟峰; 石文英; 肖豆; 方闯] Department of Acupuncture and Tuina Rehabilitation, the First Affiliated Hospital of Hunan University of Chinese Medicine
摘要：
Objective: To explore whether there is a specific clinical effect of acupuncture in the treatment of chronic prostatitis. Methods: A total of 52 patients with chronic prostatitis were randomly divided into an acupuncture group (24 cases) and a placebo acupuncture group (28 cases). During the treatment, 1 case was dropped out in the placebo acupuncture group and 51 patients accomplished the clinical trial finally in two groups. In the acupuncture group, Shenshu (肾俞BL23), Zhongliao (中髎BL33), Huiyang (会阳BL35) and Sanyinjiao (三阴交SP6) were selected. In the placebo acupuncture group, the non-meridian points located lateral to BL23, BL33, BL35 and SP6 were selected, respectively. The duration of treatment was 8 weeks in each group. In the first 4 weeks of treatment, the treatment was given once every two days, three times weekly. In the last 4 weeks of treatment, the treatment was given once every three days, twice a week. Totally, 20 acupuncture treatments were required in the whole trial. Before treatment, in week 4 and 8 of treatment and in follow-up, National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) score and the comprehensive effect were evaluated in the two groups successively. Results: In week 4 and 8 of treatment, NIH-CPSI score in the acupuncture group was lower than that before treatment, respectively (both P < 0.05). In week 8 of treatment, NIH-CPSI score in the placebo acupuncture group was lower than that before treatment (P < 0.05). NIH-CPSI score in the acupuncture group was lower than the placebo acupuncture group in week 8 of treatment (P < 0.05). In follow-up, NIH-CPSI score of the two groups all decreased as compared with the score before treatment (both P < 0.05), and the score in the acupuncture group was lower than the placebo acupuncture group (P < 0.05). In the comparison of comprehensive effect, the total effective rate was 91.7% in the acupuncture group and was 74.1% in the placebo acupuncture group. The therapeutic effect in the acupuncture group was better than that of the placebo acupuncture group (P < 0.05). Conclusion: Acupuncture relieves pelvic pain and urination symptoms and has a certain of long-term effect in patients with chronic prostatitis.
作者机构：
[Wei L.; Hui Y.; Shihui L.; Jinxi W.] Medical Innovation Experimental Center, First Hospital of Hunan University of Chinese Medicine, Changsha, 410000, China;[Xiahong Y.] First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, 410000, China
通讯机构：
[Wei, L.] M;Medical Innovation Experimental Center, China
摘要：
Objective:To study the effect of effective components of Yixintai(益心泰)on left ventricular hypertrophy and cardiomyocyte apoptosis in rabbits with chronic heart failure(CHF)and its mechanism.Methods:Twenty New Zealand rabbits were randomLy selected from 120 rabbits as th...MORE Objective:To study the effect of effective components of Yixintai(益心泰)on left ventricular hypertrophy and cardiomyocyte apoptosis in rabbits with chronic heart failure(CHF)and its mechanism.Methods:Twenty New Zealand rabbits were randomLy selected from 120 rabbits as the sham operation group,and the remaining 100 rabbits were used to establish the rabbit model of CHF by coarctation of the abdominal aorta and intragastric administration of propylthiouracil.The rabbits successfμLly modeled were randomLy divided into a model control group,2.1 g/kg,4.2 g/kg,and 8.4 g/kg of Yixintai effective components groups,and a 2.75 mg/kg losartan potassium group.An equal volume of distilled water was orally administrated to rabbits in the model control group and sham operation control group,once per day,for 4 weeks.After treatment,the myocardial pathological structure,the level of N-terminal B-type natriuretic peptide(NT-pro BNP)in the serum,cardiacμLtrasound parameters,hemodynamic parameters,left ventricular mass index,cardiomyocyte apoptosis,and the protein and mRNA expression levels of c-Jun N-terminal kinase(Cjun),serum response element(Cfos)in myocardial tissues were observed.Results:As compared with the sham operation group,the serum level of NT-pro BNP in the model control group was significantly increased(P<0.01),the left ventricular ejection fraction(LVEF)and the left ventricular systolic pressure(LVSP)were decreased(P<0.01),the left ventricular posterior wall thickness(LVPWT)and the left ventricular end-diastolic pressure(LVEDP)were increased(P<0.01),the left ventricular mass index and the cardiomyocyte apoptosis were increased(P<0.01),and the protein and mRNA expression of Cjun and Cfos in myocardial tissues were up-regulated(P<0.01).As compared with the model control group,the serum level of NT-pro BNP in each treatment group was significantly decreased(P<0.01),the LVEF and the LVSP were increased(P<0.01),the LVPWT and the LVEDP were decreased(P<0.01),the cardiomyocyte apoptosis were decreased(P<0.01),and the protein and mRNA expression of Cjun and Cfos in myocardial tissues were down-regulated(P<0.05,P<0.01).The left ventricular mass index in the 2.75 mg/kg losartan potassium group and 4.2 g/kg and 8.4 g/kg of Yixintai effective components groups were significantly decreased(P<0.01).Conclusion:The effective components of Yixintai can inhibit the protein and mRNA expressions of Cjun and Cfos in myocardial tissues of rabbits with CHF,reduce left ventricular hypertrophy and cardiomyocyte apoptosis,and improve heart failure.FEWER
作者机构：
[刘梨; 龚志贤] The First Affiliated Hospital/the First Clinical College of Hunan University of Chinese Medicine, Changsha 410007, China;[周兰; 黎铭玉; 艾坤; 周巍; Zhou, Lan] College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208;[王文怡] College of Nursing, Hunan University of Chinese Medicine, Changsha 410208
作者机构：
[张田田; 胡国恒; 谢丽华; 商燕] Hunan University of Chinese Medicine,,The First Hospital of Hunan University of Chinese Medicine;[蔺晓源] The First Hospital of Hunan University of Chinese Medicine
关键词：
网络药理学;分子对接;益气活血方;脑缺血;机制
摘要：
脑缺血（cerebral ischemia, CI）具有高发病率、高致畸率,是全球第二大致死疾病。中药（traditional Chinese medicine,TCM）益气活血方（YQHX）在临床治疗CI发挥一定疗效,但其机制尚不清楚。本文基于网络药理学和分子对接探讨其作用机制。通过TCMSP和CNKI数据库获取益气活血方活性成分;利用Pharmmapper数据库获得成分靶点信息;检索OMIM、Gene Cards、DisGeNET数据库得到脑缺血的疾病靶点; Venn图得到交集靶点, Cytoscape可视化结果,插件MCODE获得核心靶点;使用Metascape数据库对核心靶点进行GO和KEGG通路富集分析;选取前20条KEGG通路富集通路用Cytoscape构建"成分-靶点-通路"图;排名前10的成分与前5的靶点信息,利用AutoDockVina软件进行分子对接验证,用PyMoL、Ligplus软件可视化结果。筛选得到益气活血方活性成分83个,相应靶点432个,疾病相关靶点2005个,药物与疾病的交集靶点140个;通过GO生物功能分析和KEGG通路富集分析得到507条生物功能条目及141条信号通路。KEGG通路富集分析主要参与细胞增殖、粘附、迁移等过程;分子对接结果显示,筛选的关键成分与核心靶点皆具备较强的结合活性,其中EGFR、MAP2K1、KDR与丹参新酮I、丹参酮二酚结合较为稳定。益气活血方治疗脑缺血主要生物学机制可能通过酪氨酸激酶受体相关的信号通路发挥作用,也是对中医"益气活血,血脉新生"理论的完善。
作者机构：
[蒋司晨; 何阳; 陈镇] Department of Pharmacy, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[邓桂明] School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[王胜玫; 欧阳林旗] Department of Pharmacy, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China
