作者机构：
[侯芬; 李平] Department of Radiology, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;[李恒国] Medical Imaging Center, The First Affiliated Hospital of Jinan University, Guangzhou, 510632, China;[刘霞; 周智凤] Neuropsychiatric Imaging Center, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen, 518000, China
通讯机构：
[Liu, X.] N;Neuropsychiatric Imaging Center, China
摘要：
In the peripheral nervous system, the activation of Sirtuin 1 can improve insulin resistance; however, the role played by Sirtuin 1 in the central nervous system remains unknown. In this study, rat models of diabetes mellitus were generated by a single injection of streptozotocin. At 8 weeks after streptozotocin injection, the Morris water maze test and western blot assays confirmed that the diabetic model rats had learning and memory deficits, insulin resistance, and Sirtuin 1 expression could be detected in the hippocampus. Insulin and the insulin receptor inhibitor S961 were intranasally administered to investigate the regulatory effects of insulin signaling on Sirtuin 1. The results showed that insulin administration improved the impaired cognitive function of diabetic model rats and increased the expression levels of phosphorylated insulin receptor, phosphorylated insulin receptor substrate 1, and Sirtuin 1 in the hippocampus. Conversely, S961 administration resulted in more severe cognitive dysfunction and reduced the expression levels of phosphorylated insulin receptor, phosphorylated insulin receptor substrate 1, and Sirtuin 1. The Sirtuin 1 activator SRT2104 and the inhibitor Sirtinol were injected into the lateral ventricle, which revealed that the activation of Sirtuin 1 increased the expression levels of target of rapamycin complex 1, phosphorylated cAMP-response element-binding protein, and brain-derived neurotrophic factor. Hippocampal dendritic length and spine density also increased in response to Sirtuin 1 activation. In contrast, Sirtinol decreased the expression levels of target of rapamycin complex 1, phosphorylated cAMP-response element-binding protein, and brain-derived neurotrophic factor and damaged the dendritic structure. These findings suggest that the Sirtuin 1 signaling pathway plays an important role in the development of insulin resistance-related cognitive deficits in diabetic rats. This study was approved by the Animal Ethics Welfare Committee of the First Affiliated Hospital of Hunan University of Chinese Medicine (approval No. ZYFY201811207) in November 2018.
作者机构：
[黄敖; 张帅; 高飞; 谈园; 罗宏] Department of Dermatology, The First Hospital of Changsha, Changsha, 410005, China;[马乐] Department of Dermatology, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410005, China
通讯机构：
[Luo, H.] D;Department of Dermatology, China
作者机构：
[李春艳; 赵洪庆; 邹蔓姝; 黄会珍; 王叶情; 刘灿; 王宇红] Hunan University of Chinese Medicine, National Key Lab Breeding Base of Chinese Medicine Powder and Innovative Medicine/Technology Innovation Center, Changsha, 410208, China;[李强翔] Ningxia Medical University Affiliated People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, 750001, China
作者机构：
[游天; 范春华; 邓惠婷; 李平; 高辉] Department of Radiology, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China;College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China;[张堃] Department of Radiology, The First Hospital of Hunan University of Chinese Medicine, Changsha, 410007, China, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
[Zhang, K.] D;Department of Radiology, China
作者：
Gong Hui;Kuang Gaoyan;Wu Yongrong;Lu Fangguo;Deng Yihui;...
期刊：
中医杂志,2021年41(3):447-454 ISSN：1001-1668
作者机构：
[Gong Hui] Hunan Univ Chinese Med Integrated Chinese & Weste, Dept Oncol, Changsha 410007, Peoples R China.;[Kuang Gaoyan; Wu Yongrong] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Orthoped, Changsha 410007, Peoples R China.;[Deng Yihui; Lu Fangguo] Hunan Univ Chinese Med, Postdoctoral Res Stn, Changsha 410208, Peoples R China.;[Wang Hengxin; He Yanlin] Hinye Pharmaceut Co Ltd, Postdoctoral Res Workstn, Changsha 410331, Peoples R China.
摘要：
OBJECTIVE: To investigate the efficacy of Zhuifeng tougu capsules, ZFTG) in the treatment of rheumatoid arthritis (RA) in rats and study its mechanism, focusing on the toll-like receptor 2/ 4-nuclear factor kappa-B (TLR2/4-NF-kappa B) signaling pathway. METHODS: Type. collagen and an artificial climate box were used to construct the rat model of collagen-induced arthritis with wind-cold-dampness arthralgia syndrome. The rats were divided randomly into a control group, wind-cold-dampness syndrome model group, and high-, medium-, and low-dose ZFTG groups. The methotrexate (MTX) control group was treated with the corresponding drug intervention for 28 d. The joint temperature, pain threshold, joint swelling degree, and arthritis index (AI) score were measured. The production of C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and rheumatoid factors (RFs) in the blood was detected by enzyme-linked immunosorbent assay. The protein expression of TLR2, TLR4, and NF-kappa B in synovial tissues was detected by Western blotting, and the mRNA expression of TLR2, TLR4, and NF-kappa B was detected by real-time polymerase chain reaction. RESULTS: Compared with the model group, the joint temperature in each treatment group, the MTX control group, and MTX group recovered, the degree of foot swelling, pain threshold, AI score decreased, serum CRP, ESR, RF level and the levels of TLR2, TLR4, and NF-kappa B in synovial tissue were decreased (P < 0.05). Among them, the curative effect in the medium-dose and MTX groups was more evident (P < 0.01). CONCLUSION: ZFTG has a significant effect on RA in rats, and its mechanism may involve regulating CRP levels, the ESR, and RFs via the TLR2/4-NF-kappa B signaling pathway. (C) 2021 JTCM. All rights reserved.
作者机构：
[王宇红; 赵洪庆; 孟盼; 马明玥; 李姿蓉; 韩远山; 张尚霞; 吴梦瑶] Hunan University of Traditional Chinese Medicine, National Key Laboratory Breeding Base of Chinese Medicine Powders and Innovative Drugs/Technology Innovation Center, Changsha, 410208, China
