作者机构：
[魏高文; 陈书; 朱旭] Department of Epidemiology and Health Statistics, College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Hunan, Changsha, 410208, China;[魏歆然] Department of Acupuncture and Massage Rehabilitation, The First Hospital of Hunan University of Chinese Medicine, Hunan, Changsha, 410007, China
通讯机构：
[Wei, G.-W.] D;Department of Epidemiology and Health Statistics, Hunan, China
作者机构：
[蔡喆燚] Department of Critical Care Medicine, The First Hospital of Hunan University of Chinese Medicine, Hunan, Changsha, 410007, China;[詹昱新] Department of Nursing, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, 430022, China;[蔡亚宏] Department of Nephrology, The First Hospital of Hunan University of Chinese Medicine, Hunan, Changsha, 410007, China;[陈献; 余艳兰] Department of Nursing, The First Hospital of Hunan University of Chinese Medicine, Hunan, Changsha, 410007, China;[桂玲莉] The Second Ward, Department of Radiotherapy and Chemotherapy for Thoracic Oncology, Zhongnan Hospital of Wuhan University, Hubei, Wuhan, 430071, China
通讯机构：
[Zhan, Y.-X.] D;Department of Nursing, Hubei, China
作者机构：
[Qian LI; Yuan FANG; Xiao-rong CHANG; Mai-lan LIU] School of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha Hunan 410208, China (湖南中医药大学针灸推拿学院, 湖南长沙, 410208, 中国);[Hai-yan XU] Hunan Changsha County Xingcha Hospital, Changsha Hunan 410100, China (湖南长沙县星沙医院, 湖南长沙, 410100, 中国);[Bo LI] Radiology Department of the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha Hunan 410005, China (湖南中医药大学第一附属医院放射科, 湖南长沙, 410005, 中国)
通讯机构：
[Mai-lan LIU] S;School of Acupuncture, Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha Hunan 410208, China (湖南中医药大学针灸推拿学院, 湖南长沙, 410208, 中国)
关键词：
Zusanli (足三里ST36)
摘要：
Objective: To explore the differences in the central response after acupuncture and moxibusbution at Zusanli (足三里ST36) in treatment of functional dyspepsia (FD) based on the analysis of fractional amplitude of low frequency fluctuation (fALFF) of resting-state functional magnetic resonance imaging (rfMRI). Methods: A total of 60 patients with FD were randomized into an acupuncture group and a moxibustion group, 30 cases in each one. In the acupuncture group, acupuncture was applied to bilateral ST36. In the moxibustion group, moxibustion was exerted at bilateral ST36. The treatment was given once a day, 5 times a week, totally for 4 weeks in each group, including 20 treatments with acupuncture or moxibustion. Separately, before and at the end of treatment, rfMRI scanning was conducted in two groups. Using data processing assistant for resting-state fMRI (DPARSF) software and MATLAB data platform, rfMRI data were collected for preprocessing and fALFF analysis. Results: Compared with the data before treatment, after treated with acupuncture at ST36 in FD patients, fALFF value was reduced in the right superior frontal gyrus, left superior frontal gyrus, left inferior frontal gyrus, right cuneus, left precuneus, right middle occipital gyrus, left middle occipital gyrus, etc., and it was increased in the left parahippocampus, right parahippocampus, left cerebellum, etc (all P < 0.01). After treated with moxibustion, the remarkable increase of fALFF was not discovered in brain areas, but fALFF decreased significantly in the left superior parietal gyrus ( P < 0.01). Compared with moxibustion at ST36, after acupuncture at ST36 in FD patients, the increase of fALFF appeared in the right putamen and the decrease of it occurred in the middle occipital gyrus, indicating the statistical significance (both P < 0.05). Conclusion: The differences in central function responses are induced in treamtent with acupuncture and moxibustion, which is probably related to the resting-state default network associated with targeted regulation and ventral attention of brain areas in functional dyspepsia.
摘要：
OBJECTIVE: To explore the effects of Qingguang'an (...) containing serum on the expression levels of autophagy related genes in the transforming growth factor beta 1 (TGF-beta 1)-activated human Tenon's fibroblasts (HTFs). METHODS: (a) Primary HTFs were stimulated by TGF-beta 1 and underwent immunohistochemistry, which established a cell model after Glaucoma filtration surgery (GFS). (b) The cell models were divided into 4 group: normal group (normal cells), model group (+TGF-beta 1), treatment group (+TGF-beta 1+ medicated serum), and positive control group (TGF-beta 1+ rapamycin). Then, Qingguang'an medicated serum with optimum concentration was added to the corresponding group. The autophagy positive cells were identified by the Cyto-ID autophagy detection kits under fluorescent microscope and Cytation 5 multifunctional instrument for cell imaging. And the mean fluorescence intensity of autophagy positive cells was determined by flow cytometry. The expression levels of autophagy related genes - Beclin-1, autophagy related gene 5 (ATG-5), and microtubule-associated protein 1 light chain 3 (LC-3.) were detected by quantitative reverse transcription-polymerase chain reaction and Western blot analysis. RESULTS: Compared with the normal group and the model group, the relative mRNA expression levels of autophagy-related genes (Beclin-1, ATG-5 and LC-3.) in the experimental group were notably increased (P < 0.05, P < 0.01), and with the extension of treatment time, it had an increasing trend (48 h was more obvious), which showed a certain time dependency; the protein expression levels of autophagy-related genes (Beclin-1, ATG-5, and LC-3.) were significantly increased in the experimental group (P < 0.05, P < 0.01). With the prolongation of treatment time, there was an increasing trend (48 h was relatively obvious), and it revealed a certain time dependency. CONCLUSION: The Qingguang'an medicated serum could up-regulate autophagy related genes (Beclin1, ATG5, and LC3.) in the TGF-beta 1-activated HTFs. (C) 2021 JTCM. All rights reserved.
作者机构：
[Han, Yuan-Shan; Wang, Yu-Hong; Tang, Lin; Liu, Jian; Liu, Lin] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Zhao, Hong-Qing; Yang, Hui] Hunan Univ Chinese Med, Inst Innovat & Appl Res, Changsha, Hunan, Peoples R China.;[Meng, Pan; Zhao, Hong-Qing; Yang, Hui] Hunan Univ Chinese Med, Key Lab Chinese Mat Med Power & Innovat Drugs Est, Changsha, Hunan, Peoples R China.;[Meng, Pan; Zhao, Hong-Qing; Yang, Hui] Hunan Univ Chinese Med, Domest First Class Construct Discipline Chinese M, Changsha, Hunan, Peoples R China.
通讯机构：
[Wang, Yu-Hong] H;Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
摘要：
Our previous studies have shown that glutamate and hippocampal neuron apoptosis are key signals and direct factors associated with diabetes-related depression, and structural and functional damage to the hippocampal neurovascular unit has been associated with diabetesrelated depression. However, the underlying mechanism remains unclear. We hypothesized that diabetes-related depression might be associated with the glutamate（Glu）/metabotropic glutamate receptor2/3(mGluR2/3)/phosphoinositide 3-kinase（PI3K） pathway, activated by glucocorticoid receptors in the hippocampal neurovascular unit. To test this hypothesis, rat hippocampal neurovascular unit models, containing hippocampal neurons, astrocytes, and brain microvascular endothelial cells, were treated with 150 mM glucose and 200 μM corticosterone, to induce diabetes-related depression. Our results showed that under conditions of diabetes complicated by depression, hippocampal neurovascular units were damaged, leading to decreased barrier function; elevated Glu levels; upregulated glucocorticoid receptor, vesicular glutamate transporter 3（VGLUT-3）, and metabotropic glutamate receptor 2/3(mGluR2/3) expression; downregulated excitatory amino acid transporter 1（EAAT-1） expression; and alteration of the balance of key proteins associated with the extracellular signal-regulated kinase（ERK）/glial cell-derived neurotrophic factor（GDNF）/PI3K signaling pathway. Moreover, the viability of neurons was dramatically reduced in the model of diabetes-related depression, and neuronal apoptosis, and caspase-3 and caspase-9 expression levels, were increased. Our results suggest that the Glu/mGluR2/3/PI3K pathway, induced by glucocorticoid receptor activation in the hippocampal neurovascular unit, may be associated with diabetes-related depression. This study was approved by the Laboratory Animal Ethics Committee of The First Hospital of Hunan University of Chinese Medicine, China（approval No. HN-ZYFY-2019-11-12） on November 12, 2019.
