期刊：
Journal of Drug Targeting,2022年30(7):737-752 ISSN：1061-186X
通讯作者：
Cai, X;Liu, B
作者机构：
[Cai, Xiong; Hu, Shengtao; Su, Zhaoli; Cai, X; Yi, Ouyang; Dai, Zongsun; Lin, Ye; Huang, Hong] Hunan Univ Chinese Med, Hosp 1, Inst Innovat & Appl Res Chinese Med, Changsha, Hunan, Peoples R China.;[Cai, Xiong; Hu, Shengtao; Su, Zhaoli; Cai, X; Yi, Ouyang; Dai, Zongsun; Lin, Ye; Huang, Hong] Hunan Univ Chinese Med, Hosp 1, Dept Rheumatol, Changsha, Hunan, Peoples R China.;[Liu, Bin; Liu, B; Tong, Chunyi] Hunan Univ, Coll Biol, Changsha, Hunan, Peoples R China.
通讯机构：
[Liu, B ; Cai, X ] H;Hunan Univ Chinese Med, Hosp 1, Inst Innovat & Appl Res Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Rheumatol, Changsha, Hunan, Peoples R China.;Hunan Univ, Coll Biol, Changsha, Hunan, Peoples R China.
关键词：
Rheumatoid arthritis;indomethacin;Prussian blue nanoparticles;targeted delivery;biomimetic membrane;photothermal therapy
摘要：
Rheumatoid arthritis (RA) is a systemic autoimmune disease characterised by inflammatory micro-environments in the joints. Indomethacin (IND), a conventional nonsteroidal anti-inflammatory drug (NSAID), has been used for the therapy of RA. However, the poor solubility and serious side effects of oral administration of IND significantly limit its efficacy. In this study, we have synthesized biomimetic IND-loaded Prussian blue (PB) nanoparticles (IND@PB@M@HA) with hyaluronic acid (HA) modification for increasing the solubility and targeting the ability of IND to the inflamed joints. The application of hybrid cell membranes on the NPs endowed immune escape of IND@PB@M@HA NPs, which accordingly extended the circulation time in the blood. In vitro assay demonstrated that the combination of nanomedicine and photothermal therapy produced a powerful anti-inflammatory effect by reducing the levels of inflammatory factors and cell viability of activated macrophages and NPs possessed obvious pH-responsiveness. In vivo assay demonstrated that the nanomedicine for synergistic photothermal therapy exhibited desirable pharmacodynamics and pharmacokinetic properties at ultra-low drug dosage in a rat model of adjuvant-induced arthritis, which was confirmed by inflammatory suppression, bone erosion remission, and negligible adverse effects. In summary, the proposed nanomedicine has the potential role for targeted anti-inflammatory therapy of RA.
摘要：
Inflammatory bowel disease, characterised by chronic relapsing-remitting colitis, is a significant risk factor for colorectal cancer (CRC). Previously, we showed that miR-126 functions as a tumour suppressor in CRC and is inversely correlated with tumour proliferation, metastasis and patient prognosis. In the current study, we documented a protective role for miR-126 in colitis-associated CRC (CAC) and its underlying mechanism. We detected downregulated miR-126 expression during colorectal tumorigenesis in the mouse CAC model and in specimens from patients with CRC. The deficiency of miR-126 in intestinal epithelial cells (IECs) exacerbated tumorigenesis in mice. We identified CXCL12 as a direct target of miR-126 in inhibiting the development of colitis and CAC. Moreover, miR-126 regulated the recruitment of macrophages via CXCL12 and decreased the levels of proinflammatory cytokines (IL-6, IL-12 and IL-23). In addition, IL-6 secreted by macrophages, which were regulated by cocultured transfected CRC cells, altered the proliferation and migration of colon cells. Our data suggest that miR-126 exerts an antitumour effect on CAC by regulating the crosstalk between IECs and macrophages via CXCL12-IL-6 signalling. Our study contributes to the understanding of cancer progression and suggests miR-126 as a potential therapy for CRC.
作者机构：
[Li, Xiaoya; Qiao, Bo; Tan, Zhoujin; Yu, Rong; Deng, Na] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha 410208, Peoples R China.;[Peng, Xinxin] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Pediat, Changsha 410208, Peoples R China.;[Peng, Maijiao] Hunan Univ Chinese Med, Sch Pharm, Changsha 410208, Peoples R China.
通讯机构：
[Rong Yu; Zhoujin Tan] S;School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China
关键词：
SCFAs;adenine combined with Folium sennae-induced diarrhea;characteristic bacteria;gut microbiota;intestinal inflammatory response;kidney function
摘要：
Abstract: Background: Extensive evidence suggests that gut microbiota may interact with the kidneys and play central roles in the pathogenesis of disease. However, the association of gut microbiota-kidneys in diarrhea remains unclear. Methods: A diarrhea mouse model was constructed by combining adenine with Folium sennae. We analyzed the characteristics of the gut content microbiota and short chain fatty acids (SCFAs); and explored the potential link between gut content microbiota, SCFAs, intestinal inflammatory response and kidney function. Results: Characteristic bacteria Lactobacillus intestinalis and Bacteroides acidifaciens were enriched in the gut contents of mice. The productions of SCFAs were remarkably inhibited. Model mice presented an increased trend of creatinine (Cr), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), a decreased trend of blood urea nitrogen (BUN) and secretory immunoglobulin A (SIgA). The pathological analysis proved obvious damage to the kidney structure. Lactobacillus intestinalis and Bacteroides acidifaciens exisited in the correlations with acetic acid, intestinal inflammatory response and kidney function. Conclusions: Adenine combined with Folium sennae-induced diarrhea, altered the structure and function of the gut content microbiota in mice, causing the enrichment of the characteristic bacteria Lactobacillus intestinalis and Bacteroides acidifaciens. The interactions between Lactobacillus intestinalis, Bacteroides acidifaciens and acetic acid, intestinal inflammation, and kidney function might be involved in the process of gut-kidney impairment in adenine, combined with Folium sennae-induced diarrhea. Keywords: gut microbiota; adenine combined with Folium sennae-induced diarrhea; characteristic bacteria; SCFAs; intestinal inflammatory response; kidney function
摘要：
BACKGROUND: Osteoporosis (OP) has emerged as a major global public health issue due to its high prevalence, unknown pathogenesis, and lack of specific drugs for prevention and treatment. Studies have demonstrated that acupuncture in combination with Chinese herbal medicine (CHM) is effective in treating OP. However, there is a scarcity of experience and high-quality evidence. A network meta-analysis and systematic review were used to evaluate the efficacy and safety of acupuncture in combination with CHM for the treatment of OP. METHODS: Comprehensive search of Chinese databases such as China National Knowledge Infrastructure, VIP, Wanfang, China Biomedical Literature Database and English databases for example PubMed, Cochrane Library, EMbase, etc. The search period was extended from the creation of the database to November 2022. All randomized controlled trials on acupuncture in combination with CHM in dealing with OP were collected. After literature analysis and data extraction, the Cochrance system was used to evaluate the high quality of the included articles and Stata 14.0 was used for the network meta-analysis. RESULTS: The current systematic review and network meta-analysis will provide the effectiveness and safety of acupuncture in combination with CHM in dealing with OP. CONCLUSION: The research will provide reliable evidence for the clinical use of acupuncture in combination with CHM in dealing with OP.
作者机构：
[Shao, Le; Yi, Jian; Chen, Bowei] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Li, Ya; Guo, Zhihua; She, Yan] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Wu, Qibiao] Macau Univ Sci & Technol, Fac Chinese Med, State Key Lab Qual Res Chinese Med, Taipa, Macau, Peoples R China.;[Wu, Qibiao] Zhuhai MUST Sci & Technol Res Inst, Zhuhai, Guangdong, Peoples R China.;[Guo, Zhihua] Hunan Univ Chinese Med, Ave Xue Shi, Changsha, Hunan, Peoples R China.
关键词：
Buyang Huanwu decoction;Meta-analysis;Sequelae of stroke;Systematic review;Traditional Chinese medicine
摘要：
BACKGROUND: Buyang Huanwu decoction (BYHWD) is a famous traditional Chinese formula that has been widely prescribed for sequelae of stroke in China. However, the efficacy and safety of BYHWD in treating sequelae of stroke have never been systematically evaluated. PURPOSE: To evaluate the effectiveness and safety of BYHWD in the treatment of sequelae of stroke. STUDY DESIGN: A Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA)-compliant systematic review and meta-analysis of randomized clinical trials (RCTs). MATERIALS AND METHODS: A systematic review and meta-analysis was performed in accordance with the PRISMA guidelines. Five common electronic databases were searched for relevant RCTs from their inception until May 20, 2022. The Cochrane risk-of-bias tool was used to evaluate the methodological quality and the risk of bias of the included RCTs. Review Manager 5.4 was used to analyse all the data obtained. The clinical effective rate (CER) was the primary outcome, and National Institutes of Health Stroke Scale (NIHSS) and Fugl-Meyer Assessment (FMA) scores were the secondary outcomes. The Grading of Recommendations, Assessment, Development and Evaluations (GRADE) system was used to evaluate the quality of evidence for each outcome. RESULTS: Thirty-two clinical studies that recruited 2,527 eligible patients were included in this meta-analysis. The results of the meta-analysis suggested that compared with conventional treatment alone, the addition of BYHWD significantly improved the CER (RR=1.24, 95% CI: 1.20-1.29, p < 0.00001), decreased the NIHSS score (MD=-5.42, 95% CI: -5.87-4.97, p < 0.00001), and increased the FMA score (MD=17.28, 95% CI: 15.12-19.45, p < 0.00001). There were no reported adverse events in the included studies. Most results were robust, and the quality of evidence was moderate. CONCLUSION: Our study is the first meta-analysis of RCTs evaluating the effects of BYHWD on sequelae of stroke. The addition of BYHWD to conventional treatment for sequelae of stroke significantly improved the CER and promoted neurological rehabilitation in patients, and there were no reported adverse events associated with this combination therapy. The findings of our study support the use of BYHWD as an adjunct treatment to conventional treatment in this clinical population. However, due to the limitations of the included clinical trials, high-quality clinical trials with longer follow-ups are needed to assess the long-term effectiveness and safety of BYHWD for treating the sequelae of stroke.
摘要：
A chalcone-flavonone type biflavonoid, trichocladabiflavone A (1), along with eight known biflavonoids (2–9) were isolated from the 70% EtOH extract of Selaginella trichoclada. Their structures were elucidated by extensive spectroscopic analyses. Compound 1 was the first chalcone-flavonone type biflavonoid reported in the genus Selaginella. Moreover, compound 1 exhibited moderate cytotoxicity against DU145, MCF-7 and PC3 human cancer cell lines.
摘要：
BACKGROUND: Although anti-inflammatory and immunomodulatory measures have delayed the progression of ulcerative colitis (UC) to a certain extent, the adverse drug reactions and recurrence after recovery still trouble clinicians. Acupoint catgut embedding is a possible alternative strategy for the treatment of UC, but its clinical efficacy remains controversial. Therefore, this study systematically evaluated the clinical efficacy and safety of acupoint catgut embedding compared with conventional western medicine in the treatment of UC. METHODS: VIP, Wanfang, China National Knowledge Infrastructure, China Biology Medicine, PubMed, Embase, Web of Science, the Cochrane Library databases were searched. And the publication time of the literature was limited from the time that the database was established to February 2022. Two researchers independently screened the literature, extracted data, and assessed risk of bias as required. Meta-analysis was performed with Revman 5.3. Trial sequential analysis (TSA) was performed with TSA 0.9.5.10 Beta. Publication bias was assessed by Stata 15.0. And evidence quality was appraised with GRADEpro3.6. RESULTS: A total of 10 studies were listed, with a total sample size of 782 cases. Meta-analysis showed that compared with conventional western medicine, acupoint catgut embedding can effectively improve the total effective rate of clinical symptoms (relative risk [RR] = 1.16, 95% confidence interval [CI] = [1.09,1.24], P < .00001), endoscopic total effective rate (RR = 1.16, 95%CI = [1.08,1.25], P < .0001), clinical symptom cure rate (RR = 1.80, 95%CI = [1.37,2.38], P < .0001), and endoscopic cure rate (RR = 1.97, 95%CI = [1.36,2.86], P = .0004) of UC, but the adverse event rate (RR = 0.20, 95%CI = [0.01,4.00], P = .29) was similar. Trial sequential analysis indicated that the efficacy endpoint was conclusive. Harbord test confirmed no significant publication bias. The quality of evidence for these outcomes ranges from low to medium. CONCLUSION: The clinical efficacy of acupoint catgut embedding in the treatment of UC is superior to that of conventional western medicine, and the safety may be equivalent to that of conventional western medicine, which has the value of further research and exploration.
摘要：
BACKGROUND: Chronic atrophic gastritis (CAG) is an important stage of precancerous lesions of gastric cancer, and also a key period of drug intervention. However, there is still a lack of drugs to maintain the treatment of CAG until the advent of moluodan. OBJECTIVE: This study was conducted to assess the clinical efficacy of moluodan in the treatment of CAG by meta-analysis and trial sequential analysis. METHODS: China National Knowledge Infrastructure, China Biology Medicine, VIP, Wanfang, Embase, PubMed, the Cochrane Library, and Web of Science databases were searched, all with the time limit from database establishment to July 2022. The published randomized controlled trials of moluodan for CAG were conducted for meta-analysis and trial sequential analysis. RESULTS: 7 studies with a total sample size of 1143 cases were included. Compared to folic acid/vitamins, moluodan alone significantly increased the effective rate of pathological detection (relative risk [RR] = 1.73, 95% confidence interval [95%CI] = [1.48,2.02], P < .00001), and moluodan in combination with folic acid/vitamins significantly increased the effective rates of pathological detection (RR = 1.37, 95%CI = [1.23,1.52], P < .00001), gastroscopy (RR = 1.37, 95%CI = [1.18,1.60], P < .0001) and symptoms (RR = 1.25, 95%CI = [1.13,1.38], P < .0001). Harbord regression showed no publication bias (P = .22). Quality of evidence evaluation demonstrated moderate quality of evidence for all indicators. CONCLUSIONS: Moluodan can improve the effective rates of pathological examination, gastroscopy and symptoms in patients with CAG, and play a role in slowing down the disease progression and reducing clinical symptoms. It may be a potential drug for the treatment of CAG and has the value of further exploration.
摘要：
Tumor chemoresistance is often a major cause for the failure of chemotherapy. The resistance of hepatocellular carcinoma (HCC) cells to sorafenib significantly limits its therapeutic effect in HCC patients. For the first time, we found that FXYD domain-containing ion transport regulator 5 (FXYD5) is highly expressed in sorafenib-resistant HCC cells. In addition, the protein expression level of FXYD5 was markedly higher in HCC tissues than in paracancerous tissues. Remarkably, downregulation of FXYD5 expression in Huh7/sora cells reversed their resistance to sorafenib. Moreover, overexpression of FXYD5 reduced the sensitivity of HCC cells to sorafenib, while the downregulation of its expression in HCC cells had the opposite effect. We also found abnormal activation of the Akt/mTOR signaling pathway in Huh7/sora cells. Furthermore, MK2206, an Akt inhibitor, was found to significantly increase the sensitivity of HCC cells to sorafenib. More importantly, the expression level of p-Akt was positively correlated with the expression of FXYD5 in HCC tissues. Therefore, mechanistically, FXYD5 enhances the resistance of HCC cells to sorafenib by activating the Akt/mTOR signaling pathway. In conclusion, this study showed that the activation of the FXYD5/Akt/mTOR signaling axis plays key role in the resistance of HCC cells to sorafenib, and FXYD5 may represent a new potential target for HCC therapy.
作者机构：
[Zeng, Liuting; Xu, Hao; Zeng, Jinsong; Yang, Kailin; Wang, Shanshan; Ge, Anqi; Ge, Jinwen; Yuan, Xiao] Hunan Univ Chinese Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Zeng, Liuting] Chinese Academy of Medical Sciences & Peking Union, Peking Union Med Coll Hosp, Natl Clin Res Ctr Dermatol & Immunol Dis NCRC DID, Key Lab Rheumatol & Clin Immunol Minist Educ,Dept, Beijing, Peoples R China.;[Zeng, Jinsong; Ge, Anqi] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Jinwen Ge] K;Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha, China
关键词：
Ischemic stroke;Ferroptosis;Oxidative stress;Natural pharmacological active component
摘要：
Cerebrovascular diseases, such as ischemic stroke, pose serious medical challenges worldwide due to their high morbidity and mortality and limitations in clinical treatment strategies. Studies have shown that reactive oxygen species (ROS)-mediated inflammation, excitotoxicity, and programmed cell death of each neurovascular unit during post-stroke hypoxia and reperfusion play an important role in the pathological cascade. Ferroptosis, a programmed cell death characterized by iron-regulated accumulation of lipid peroxidation, is caused by abnormal metabolism of lipids, glutathione (GSH), and iron, and can accelerate acute central nervous system injury. Recent studies have gradually uncovered the pathological process of ferroptosis in the neurovascular unit of acute stroke. Some drugs such as iron chelators, ferrostatin-1 (Fer-1) and liproxstatin-1 (Lip-1) can protect nerves after neurovascular unit injury in acute stroke by inhibiting ferroptosis. In addition, combined with our previous studies on ferroptosis mediated by natural compounds in ischemic stroke, this review summarized the progress in the regulation mechanism of natural chemical components and herbal chemical components on ferroptosis in recent years, in order to provide reference information for future research on ferroptosis and lead compounds for the development of ferroptosis inhibitors.
通讯机构：
[Houpan Song] H;[Qinghua Peng] T;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, 410007, China<&wdkj&>Hunan Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China<&wdkj&>Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China<&wdkj&>Hunan Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan, 410208, China
通讯机构：
Institute of Liver Diseases, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
关键词：
Cirrhosis;Entecavir;Fuzheng huayu tablet;Hepatitis B
通讯作者：
Desheng Zhou<&wdkj&>Bo Chen<&wdkj&>Haijun Tu
作者机构：
[Xie, Shuting; Liu, Mincong; Wang, Ying; Li, Hai; Chen, Bo; Ma, Ming; Min, Ke] Hunan Normal Univ, Coll Chem & Chem Engn, Key Lab Chem Biol & Tradit Chinese Med Res, Key Lab Phytochem R&D Hunan Prov,Minist Educ, Changsha 410081, Peoples R China.;[Zhou, Desheng] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Peoples R China.;[Tu, Haijun] Hunan Univ, Coll Biol, Changsha 410082, Peoples R China.
通讯机构：
[Desheng Zhou] T;[Bo Chen] K;[Haijun Tu] C;Key Laboratory of Phytochemical R&D of Hunan Province, Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research of Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China<&wdkj&>The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China<&wdkj&>College of Biology, Hunan University, Changsha 410082, China
摘要：
Abstract: Gastrodiae rhizoma (GR) formula granules and preparations have been used as a popular traditional Chinese medicine for clinical treatment since they have good pharmacological activity to treat nervous system diseases. Gastrodin and parishins have been the main active components in aqueous extracts for GR formula granules, but their pharmacological activities and metabolism are different. For quality control of the extracts, the extraction conditions should be investigated to accurately control the contents of two kinds of components. In this paper, the transfer rate of six index components (including gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, and parishin E) obtained by HPLC were used as indicators to investigate the effect of pH on the GR extraction process. The results demonstrated that pH is a key factor for preventing transforming parishins into gastrodin and maintaining high content of parishins in the extracts. It can be concluded that the weak acid environment could improve the transfer rate of parishins, thus ensuring the gastrodin and parishins consistency between GR raw materials and its aqueous extracts. Therefore, pH is an essential condition for accurate quality control of the extracts. Keywords: Gastrodiae rhizoma; gastrodin; parishins; pH; HPLC
作者机构：
[Qiu, Jingyue; Xiong, Meng; Zhong, Meiqi; Yu, Chang; Peng, Qinghua; Ren, Baoping; Song, Houpan] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost Res Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Qiu, Jingyue; Xiong, Meng; Zhong, Meiqi; Yu, Chang; Ren, Baoping; Zeng, Meiyan; Song, Houpan] Hunan Univ Chinese Med, Coll Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Peng, Qinghua] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Ou, Chen; Peng, Qinghua] Hunan Univ Chinese Med, Hunan Prov Key Lab Prevent & Treatment Ophthalmol, Changsha 410208, Hunan, Peoples R China.;[Zeng, Meiyan] Hunan Univ Chinese Med, Coll Tradit Chinese Med, 300 Xueshi Rd, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Meiyan Zeng] C;[Qinghua Peng] H;College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>Hunan Provincial Key Laboratory of Diagnostic Research in Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China<&wdkj&>The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China<&wdkj&>Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with TCM, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China
关键词：
Duodenal ulcer;HQJZT;Inflammation;NF-κB;STAT;Traditional Chinese Medicine
摘要：
Huang-Qi-Jian-Zhong-Tang (HQJZT) is a well-known traditional Chinese herbal formulation. This study aimed to investigate the duodenoprotective properties of HQJZT against Indomethacin (IND)-induced duodenal ulceration in rats, and the mechanisms involved, particularly through NF-kappa B and STAT signaling pathways. Our results showed that HQJZT completely protected the duodenal mucosa from ulceration caused by IND, as indicated by improved macroscopic and histological appearances. There was a significant decrease in ulcer index and microscopic score, an increase in villus height and crypt depth, and a normalization of the tissue architecture of the duodenum in rats following HQJZT treatment. Blood flow into the duodenal mucosa was significantly increased after HQJZT administration. HQJZT significantly increased PGE2 and NO levels in the duodenal mu-cosa. A significant reduction in the production of pro-inflammatory cytokines IL-1 beta, IL-6, and TNF-alpha was observed in the duodenal mucosa under treatment with HQJZT. Mechanistically, the administration of HQJZT significantly lowered the duodenal protein expression of inflammation-related genes, including p-NF-kappa B and p-I kappa B beta, compared with the ulcer control group. Furthermore, the STAT signaling pathway-related protein markers p-JAK and p-STAT were significantly reduced in the HQJZT (1.30 and 2.60 g/kg) groups. As a result of these findings, HQJZT alleviates duodenal mucosal ulcers caused by IND. A protective effect of HQJZT on duodenal ulcers is attributed to its ability to improve mucosal blood flow, stimulate the production of cytoprotective mediators, minimize proinflammatory cytokines, and block the activation of NF-kappa B and STAT signaling pathways.
摘要：
Osteoarthritis (OA) is a common degenerative disease with few treatments. In traditional Chinese medicine (TCM), Radix Angelicae biseratae (RAB) is commonly used to treat OA. Nodakenin (Nod) is one main coumarin active component in RAB and exhibits anti-inflammatory, anti-oxidative, and anti-apoptotic effects. Reactive oxygen species (ROS) produced by mitochondria play a vital role in the pathogenesis of OA. We hypothesized that Nod might ameliorate cartilage degradation and inflammatory responses by regulating the mitochondrial Drp1/ROS/NLRP3 axis. With this, the effects of Nod on a mouse model of knee OA and activated primary chondrocytes were assessed. The results showed that Nod intervention improved bone volume, lowered trabecular separation, and increased trabecular number in the subchondral bone. Nod decreased the Osteoarthritis Research Society International (OARSI) scores and increased collagen II-positive areas in the articular cartilage of the tibial plateau. Compared with OA mice, Nod-treated animals exhibited lower levels of inflammatory factors in the serum and synovitis of the knee joint. In vitro results indicated that Nod suppressed dynamin-related protein 1 (Drp1) phosphorylation and massive ROS production by Drp1-dependent mitochondrial fission in lipopolysaccharide-stimulated chondrocytes. Moreover, Nod inhibited the mRNA levels of inflammatory cytokines (COX 2, IL-1β, and TNF-α), nod-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, and matrix metalloproteinase 13 expression in activated chondrocytes. In conclusion, Nod attenuates cartilage degradation and inflammatory responses in mice with OA by regulating the mitochondrial Drp1/ROS/NLRP3 axis, suggesting its potential for OA therapy.
摘要：
BACKGROUND: Alzheimer disease (AD) is a common cause of dementia, and there are still a lack of treatment options to reverse or prevent disease progression. Existing evidence shows that acupuncture has advantages in the treatment of AD, but whether the efficacy of acupuncture belongs to the placebo effect remains controversial, and there is no strict systematic review and network meta-analysis to evaluate the efficacy and safety of acupuncture combined with Western medicine in the treatment of AD. METHODS: From the inception to February 2023, the Embase, Latin American and Caribbean Health Sciences Literature, Medline, the Cochrane Collaboration's Controlled Clinical Trials, Scopus, China Biomedical Literature Database, Wanfang Database, China National Knowledge Infrastructure, and Australian Medical Index will be searched using the key phrases "acupuncture," "warm needling," "electroacupuncture," "Alzheimer disease," and "cohort" for all relevant studies. Quality assessment of all studies included in this review will be independently assessed by 2 reviewers using the Cochrane Collaborations tool. When significant heterogeneity is indicated, we will find the source of heterogeneity by subgroup or sensitivity analysis. DISCUSSION: This study will evaluate the efficacy of acupuncture combined with Western medicine in improving cognitive function and activities of daily living in AD patients. The results of this study will verify whether the efficacy of acupuncture in the treatment of AD belongs to the placebo effect, which will also provide a reference for the clinical use of acupuncture combined with Western medicine in the treatment of AD.
作者机构：
[刘梨; 龚志贤] The First Affiliated Hospital/the First Clinical College of Hunan University of Chinese Medicine, Changsha 410007, China;[周兰; 黎铭玉; 艾坤; 周巍; Zhou, Lan] College of Acupuncture-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208;[王文怡] College of Nursing, Hunan University of Chinese Medicine, Changsha 410208
期刊：
Anais Brasileiros de Dermatologia,2022年97(4):458-466 ISSN：0365-0596
通讯作者：
Haibo Li<&wdkj&>Jianda Zhou
作者机构：
[Li, Haibo; Li, Xu; Zhou, Jianda; Chen, Lei; Cao, Ke; Fang, Yuan] Cent South Univ, Xiangya Hosp 3, Dept Plast Surg, Changsha, Hunan, Peoples R China.;[Fang, Yuan] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Plast & Reconstruct Surg, Shanghai, Peoples R China.;[Wang, Xin] Cent South Univ, Xiangya Hosp 2, Changsha, Hunan, Peoples R China.;[Xiong, Wu] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Peoples R China.;[Zhang, Xi] Hunan Univ Chinese Med, Hunan Brain Hosp, Clin Med Sch, Changsha, Hunan, Peoples R China.
通讯机构：
[Haibo Li; Jianda Zhou] D;Department of Plastic Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China<&wdkj&>Department of Plastic Surgery, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China
摘要：
Background: UVB irradiation can cause acute damage such as sunburn, or photoaging and melanoma, all of which are major health threats. Objective: This study was designed to investigate the mechanism of skin photoaging induced by UVB radiation in mice through the analysis of the differential expression of miRNAs. Methods: A UVB irradiation photoaging model was constructed. HE and Masson special stains were used to examine the modifications in the epidermis and dermis of mice. The miRNA expression profiles of the mouse skin model exposed to UVB radiation and the normal skin of mice were analyzed using miRNA-sequence analysis. GO and Pathway analysis were employed for the prediction of miRNA targets. Results: A total of 23 miRNAs were evaluated for significantly different expressions in comparison to normal skin. Among them, 7 miRNAs were up-regulated and 16 were down-regulated in the skin with photoaging of mice exposed to UVB irradiation. The differential expression of miRNA is related to a variety of signal transduction pathways, among which mmu-miR-195a-5p and mitogen-activated protein kinase (MAPK) signal pathways are crucial. There was a significant differential expression of miRNA in the skin of normal mice in comparison with the skin with photoaging induced by UVB irradiation. Study limitations: Due to time and energy constraints, the specific protein level verification, MAPK pathway exploration, and miR-195a-5p downstream molecular mechanism need to be further studied in the future. Conclusions: UVB-induced skin photoaging can be diagnosed and treated using miRNA. (C) 2022 Sociedade Brasileira de Dermatologia. Published by Elsevier Espana, S.L.U.
作者机构：
[Peng, Shu-Wang] Hunan Univ Chinese Med, Hosp 1, Dept Gastrointestinal & Thyroid & Vasc Surg, Changsha, Peoples R China.;[Sheng, Jiang-Ming; Tian, Gui-Xiang; Liu, Ming-Hui; Xie, Hai-Qing] Cent South Univ, Xiangya Hosp 2, Dept Ultrasound, Changsha, Peoples R China.;[Li, Yan; Feng, Bai-Sui] Zhengzhou Univ, Affiliated Hosp 2, Dept Gastroenterol, Zhengzhou, Peoples R China.;[Peng, Ke-Ping] Hunan Univ Chinese Med, Hosp 1, Dept Otorhinolaryngol Head & Neck surg, Changsha, Peoples R China.;[Tian, Gui-Xiang] Cent South Univ, Xiangya Hosp 2, Res Ctr Ultrasonog, Changsha, Peoples R China.
通讯机构：
[Gui-Xiang Tian] D;Department of Ultrasound, The Second Xiangya Hospital of Central South University, Changsha, China<&wdkj&>Research Center of Ultrasonography, The Second Xiangya Hospital of Central South University, Changsha, China
摘要：
The pathogenesis of ulcerative colitis (UC) is unclear. House dust mite (HDM) is associated with immune inflammation in the body. This study is designed to identify the association between HDM and UC clinical symptoms. UC patients (n = 86) and non-UC control (NC) subjects (n = 64) were recruited. Colon lavage fluids (CLF) were collected from HDM skin prick test positive patients during colonoscopy, and analyzed by immunological approaches. HDM was detected in fecal samples, which was positively correlated with UC clinical symptoms. HDM-specific eosinophils and Th2 cells were detected in CLF, which could be specifically activated by exposing to HDM in the culture. Direct exposure to HDM induced eosinophil activation in the colon of UC patients. UC patients displayed elevated levels of Th2 cytokines in the serum. UC clinical symptom scores were positively correlated with serum levels of Th2 cytokines. HDM was detected in UC patients' stools, which was positively correlated with UC clinical symptoms. Direct exposure to HDM could trigger eosinophilic activation of the colon.
