作者机构：
[Zhang, Weili; Jin-si-han, E-er-man-bie-ke; Lu, ZH; Lian, Shaopu; Li, Yuan; Lu, Zhenhai; Feng, Cheng; Wang, Hao] Sun Yat Sen Univ Canc Ctr, Dept Colorectal Surg, Guangzhou 510515, Guangdong, Peoples R China.;[He, Meng; He, M] Chinese Acad Med Sci & Peking Union Med Coll, Natl Clin Res Ctr Canc, Canc Hosp, Dept Radiat Oncol,Natl Canc Ctr, Shenzhen 518116, Guangdong, Peoples R China.;[He, Meng; He, M] Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen 518116, Guangdong, Peoples R China.;[Chen, QF; Chen, Qifeng] Sun Yat Sen Univ Canc Ctr, Dept Minimally Invas Intervent Therapy, Liver Canc Study & Serv Grp, Guangzhou 510060, Guangdong, Peoples R China.;[Tai, Yi] Sun Yat Senen Univ Canc Ctr, Dept Musculoskeletal Oncol, Guangzhou 510515, Guangdong, Peoples R China.
通讯机构：
[Chen, QF ; Lu, ZH ] S;[He, M ] C;Sun Yat Sen Univ Canc Ctr, Dept Colorectal Surg, Guangzhou 510515, Guangdong, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Natl Clin Res Ctr Canc, Canc Hosp, Dept Radiat Oncol,Natl Canc Ctr, Shenzhen 518116, Guangdong, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Shenzhen Hosp, Shenzhen 518116, Guangdong, Peoples R China.
摘要：
Neutrophil extracellular traps (NETs) have been categorized as a form of inflammatory cell death mode of neutrophils (NETosis) involved in natural immunity and the regulation of adaptive immunity. More and more studies revealed the ability of NETs to reshape the tumor immune microenvironment (TIME) by limiting antitumor effector cells, which may impair the efficacy of immunotherapy. To explore whether NETs-related genes make vital impacts on Colon carcinoma (COAD), we have carried out a systematic analysis and showed several findings in the present work. First, we obtained the patient's data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) dataset, aiming to detect two NETs-associated subtypes by consensus clustering. For the purpose of annotating the roles of NETs-related pathways, gene ontology enrichment analyses were adopted. Next, we constructed a 6 novel NETs-related genes score using the Least Absolute Shrinkage and Selection Operator (LASSO) Cox regression model. We found that the NETs risk score was notably upregulated in COAD patient samples, and its levels were notably correlated with tumor clinicopathological and immune traits. Then, according to NETs-associated molecular subtypes and the risk signature, this study compared immune cell infiltration calculated through the estimate, CIBERSORT, TIMER, ssGSEA algorithms, tumor immune dysfunction, as well as exclusion (TIDE). Furthermore, we confirm that MPO(myeloperoxidase) was significantly upregulated in COAD patient samples, and its levels were significantly linked to tumor malignancy and clinic outcome. Moreover, multiplex immunohistochemistry (mIHC) spatial analysis confirmed that MPO was closely related to Treg and PD-1 + Treg in spatial location which suggested MPO may paly an important role in TIME formation. Altogether, the obtained results indicated that a six NETs-related genes prognostic signature was conducive to estimating the prognosis and response of chemo-/immuno-therapy of COAD patients.
通讯作者：
Yi Zhang<&wdkj&>Jun Jiang<&wdkj&>Yi Zhang Yi Zhang Yi Zhang<&wdkj&>Jun Jiang Jun Jiang Jun Jiang
作者机构：
[Yi Sun; Yi Sun Yi Sun Yi Sun] Department of Breast and Thyroid Surgery, Xuchang Central Hospital, Xuchang, Henan, China;[Jinhui Hu; Jinhui Hu Jinhui Hu Jinhui Hu] Department of Breast Surgery, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan, China;[Yanwu Zhang; Yanwu Zhang Yanwu Zhang Yanwu Zhang] Department of Breast Surgery, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China;[Xiaowei Qi; Peng Tang; Linjun Fan; Li Chen; Shushu Wang; Yan Liang; Ying Hu; Minghao Wang; Lin Ren; Guozhi Zhang; Xuanni Tan; Long Yuan; Junze Du; Xiujuan Wu; Yi Zhang; Jun Jiang; Xiaowei Qi Xiaowei Qi Xiaowei Qi; Peng Tang Peng Tang Peng Tang; Linjun Fan Linjun Fan Linjun Fan; Li Chen Li Chen Li Chen; Shushu Wang Shushu Wang Shushu Wang; Yan Liang Yan Liang Yan Liang; Ying Hu Ying Hu Ying Hu; Minghao Wang Minghao Wang Minghao Wang; Lin Ren Lin Ren Lin Ren; Guozhi Zhang Guozhi Zhang Guozhi Zhang; Xuanni Tan Xuanni Tan Xuanni Tan; Long Yuan Long Yuan Long Yuan; Junze Du Junze Du Junze Du; Xiujuan Wu Xiujuan Wu Xiujuan Wu; Yi Zhang Yi Zhang Yi Zhang; Jun Jiang Jun Jiang Jun Jiang] Department of Breast and Thyroid Surgery, Southwest Hospital, Army Medical University, Chongqing, China;The Eighth Medical Center of Chinese PLA General Hospital, Beijing, China
通讯机构：
[Yi Zhang; Jun Jiang; Yi Zhang Yi Zhang Yi Zhang; Jun Jiang Jun Jiang Jun Jiang] D;Department of Breast and Thyroid Surgery, Southwest Hospital, Army Medical University, Chongqing, China
关键词：
HER2‐positive breast cancer;multicenter study;neoadjuvant therapy;pyrotinib;trastuzumab
摘要：
In this multicenter, single‐arm trial, we investigated the efficacy and safety of the ECPy‐THPy regimen (epirubicin, cyclophosphamide, and pyrotinib followed by docetaxel, trastuzumab, and pyrotinib) as neoadjuvant therapy for patients with stage II–III HER2‐positive breast cancer. Our results revealed that tpCR was achieved in 107 (68.6%) out of 156 patients, which was in line with our previous pilot study and numerically higher than the tpCR rate in previous phase III KRISTINE trial and the phase III POENY trial. Abstract The objective of this multicenter, single‐arm trial (ChiCTR1900022293) was to explore the efficacy and safety of neoadjuvant therapy with epirubicin, cyclophosphamide, and pyrotinib followed by docetaxel, trastuzumab, and pyrotinib (ECPy‐THPy) in the treatment of patients with stage II–III HER2‐positive breast cancer. The present study enrolled patients with stage II–III HER2‐positive breast cancer. Epirubicin and cyclophosphamide were administrated for four 21‐day cycles, followed by four cycles of docetaxel and trastuzumab. Pyrotinib was taken orally once per day throughout the treatment period. The primary endpoint was total pathological complete response (tpCR, ypT0/is ypN0) rate in the modified intention‐to‐treat (mITT) population. In total, 175 patients were included. The tpCR rate was 68.6% (95% CI, 60.7–75.8%), while the objective response rate was 89.1%. In the post‐hoc subgroup analysis, no association between clinical characteristics and the tpCR rate was observed. The most common grade ≥3 adverse events were diarrhea (54.3%), followed by white blood cell count decreased (5.1%), and neutrophil count decreased (4.6%). In conclusion, the neoadjuvant regimen with ECPy‐THPy showed promising pathological response and clinical benefits with an acceptable safety profile in patients with stage II–III HER2‐positive breast cancer.
期刊：
Frontiers in Microbiology,2023年14:1208157 ISSN：1664-302X
通讯作者：
Song, HP;Zeng, Meiyan
作者机构：
[Song, Houpan; Yuan, Chengzhi; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;[Yuan, Chengzhi] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.;[Zeng, Meiyan; Song, Houpan; Xiong, Meng; Sun, Qifang; Yu, Chang] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhou, Sainan] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.
通讯机构：
[Zeng, MY; Song, HP ] H;Hunan Univ Chinese Med, Hunan Prov Key Lab Tradit Chinese Med Diagnost, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.
摘要：
Resistance of Helicobacter pylori (H. pylori) to antibiotics has reached alarming levels worldwide, and the efficacy of the H. pylori eradication treatment has decreased dramatically because of antibiotic resistance. To gain a more comprehensive understanding of the development status, research hotspots, and future trends related to H. pylori antibiotic resistance, we conducted a thorough retrospective analysis via the bibliometrics method. We searched the Science Citation Index Expanded of the Web of Science Core Collection for all pertinent articles on H. pylori antibiotic resistance from 2013 to 2022. R-bibliometrix, CiteSpace, and VOSviewer tools were utilized to depict statistical evaluations in order to provide an unbiased presentation and forecasts in the field. We incorporated a total of 3,509 articles related to H. pylori antibiotic resistance. Publications were inconsistent prior to 2017, but steadily increased after 2017. China generated the most papers and the United States of America received the most citations and the highest H-index. Baylor College of Medicine was the most influential institution in this field, with the highest number of publications and citations, as well as the highest H-index. Helicobacter was the most productive journal, followed by the World Journal of Gastroenterology and Frontiers in Microbiology. The World Journal of Gastroenterology had the highest citation. Graham, David Y was the most productive and cited author. Clarithromycin resistance, prevalence, gastric cancer, quadruple therapy, sequential therapy, 23S rRNA, whole genome sequencing, bismuth, and probiotics appeared with a high frequency in the keywords. The top keywords with the highest citation bursts were vonoprazan, RdxA, biofilm formation, and fatty acid chain. Our research illustrated a multi-dimensional facet and a holistic knowledge structure for H. pylori antibiotic resistance research over the past decade, which can serve as a guide for the H. pylori research community to conduct in-depth investigations in the future.
期刊：
FRONTIERS IN ONCOLOGY,2023年12:1050756 ISSN：2234-943X
通讯作者：
Cao, J.;Jiang, X.;Cho, W.C.
作者机构：
[Zhou, Fang] Hunan Univ Chinese Med, Changsha, Peoples R China.;[Zhang, Zhen; Zhou, Fang] Hunan Acad Tradit Chinese Med, Affiliated Hosp, Dept Oncol, Changsha, Peoples R China.;[Zeng, Lingfeng] Prince Wales Hosp, Carol & Richard Yu Peritoneal Dialysis Res Ctr, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China.;[Zeng, Lingfeng] Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci LiHS, Fac Med, Shatin, Hong Kong, Peoples R China.;[Chen, Xi; Zhao, Jinxi; Huang, Xiaobin; Tian, Jintao; Liu, Xujie; Yao, Huayi; Wang, Heping; Pu, Jun] Kunming Med Univ, Dept Neurosurg, Affiliated Hosp 2, Kunming, Peoples R China.
通讯机构：
[Cho, W.C.; Cao, J.] D;[Jiang, X.] K;Department of Clinical Oncology, Hong Kong;Department of Oncology, China;Kunming College of Life Science, China
摘要：
Dual-specificity phosphatase 10 (DUSP10) correlates with inflammation, cytokine secretion, cell proliferation, survival, and apoptosis. However, its role in glioma is unclear. Herein, we sought to examine the expression and the underlying carcinogenic mechanisms of DUSP10 action in glioma. DUSP10 expression in glioma was significantly higher than that in normal brain tissues. High DUSP10 expression indicated adverse clinical outcomes in glioma patients. Increased DUSP10 expression correlated significantly with clinical features in glioma. Univariate Cox analysis showed that high DUSP10 expression was a potential independent marker of poor prognosis in glioma. Furthermore, DUSP10 expression in glioma correlated negatively with its DNA methylation levels. DNA methylation level of DUSP10 also correlated negatively with poor prognosis in glioma. More importantly, DUSP10 expression correlated positively with the infiltration of B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells in glioma. Gene set enrichment analysis (GSEA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis confirmed that DUSP10 participated in signaling pathways involved in focal adhesion, TNF cascade, Th17 cell differentiation, and NF-kappa B cascade. Finally, we uncovered that DUSP10 was dramatically upregulated in glioblastoma (GBM) cells and that the knockdown of DUSP10 inhibited glioma cell proliferation and migration. Our findings suggested that DUSP10 may serve as a potential prognostic biomarker in glioma.
期刊：
Ageing Research Reviews,2023年91:102063 ISSN：1568-1637
通讯作者：
Yang, Kailin;Ge, JW;Zeng, LT
作者机构：
[Wang, Shanshan; He, Qi; Yang, Kailin; Ge, Jinwen] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;[Yang, Kailin; Ge, Jinwen] Hunan Acad Chinese Med, Changsha, Hunan, Peoples R China.;[Zeng, Liuting; Zeng, LT] Chinese Acad Med Sci & Peking Union Med Coll, Nanjing Drum Tower Hosp, Grad Sch, Peking Union Med Coll,Dept Rheumatol & Immunol, Nanjing, Peoples R China.;[Zeng, Jinsong; Ge, Anqi] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[He, Qi; Deng, Ying] Peoples Hosp Ningxiang City, Ningxiang, Peoples R China.
通讯机构：
[Zeng, LT ] C;[Yang, KL; Ge, JW ] H;Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Key Lab Hunan Prov Integrated Tradit Chinese & Wes, Changsha, Peoples R China.;Chinese Acad Med Sci & Peking Union Med Coll, Nanjing Drum Tower Hosp, Grad Sch, Peking Union Med Coll,Dept Rheumatol & Immunol, Nanjing, Peoples R China.
摘要：
Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder of the central nervous system after Alzheimer's disease. The current understanding of PD focuses mainly on the loss of dopamine neurons in the substantia nigra region of the midbrain, which is attributed to factors such as oxidative stress, alpha-synuclein aggregation, neuroinflammation, and mitochondrial dysfunction. These factors together contribute to the PD phenotype. Recent studies on PD pathology have introduced a new form of cell death known as ferroptosis. Pathological changes closely linked with ferroptosis have been seen in the brain tissues of PD patients, including alterations in iron metabolism, lipid peroxidation, and increased levels of reactive oxygen species. Preclinical research has demonstrated the neuroprotective qualities of certain iron chelators, antioxidants, Fer-1, and conditioners in Parkinson's disease. Natural plant products have shown significant potential in balancing ferroptosis-related factors and adjusting their expression levels. Therefore, it is vital to understand the mechanisms by which natural plant products inhibit ferroptosis and relieve PD symptoms. This review provides a comprehensive look at ferroptosis, its role in PD pathology, and the mechanisms underlying the therapeutic effects of natural plant products focused on ferroptosis. The insights from this review can serve as useful references for future research on novel ferroptosis inhibitors and lead compounds for PD treatment.
摘要：
Glaucoma, one of the most common ocular neurodegenerative diseases worldwide, is characterized by retinal ganglion cell (RGC) loss. There is a large body of literature that describes the neuroprotective role of melatonin against neurodegenerative diseases by regulating neuroinflammation, although the exact mechanism through which melatonin acts on RGC is still uncertain. This study assessed the protective effects of melatonin using a NMDA-induced RGC injury model, and studied the possible mechanisms involved in this process. Melatonin promoted RGC survival, improved retinal function, and inhibited the apoptosis and necrosis of retinal cells. To understand the mechanism of the neuroprotective effects of melatonin on RGC, microglia and inflammation -related pathways were assessed after melatonin administration and microglia ablation. Melatonin promoted RGC survival by suppressing microglia-derived proinflammatory cytokines, in particular TNF alpha, which in turn inhibited the activation of p38 MAPK pathway. Inhibiting TNF alpha or manipulating p38 MAPK pathway protected damaged RGC. Our results suggest that melatonin protects against NMDA-induced RGC injury by inhibiting the microglial TNF alpha-RGC p38 MAPK pathway. It should be considered a candidate neuroprotective therapy against retinal neurodegenerative diseases.
期刊：
International Journal of Colorectal Disease,2023年38(1):1-16 ISSN：0179-1958
通讯作者：
Xu, Yin;Zhu, Y
作者机构：
[Zhu, Ying; Zhou, Tao; Xu, Yin; Liu, Yaxuan; Zhu, Y; Long, Dan] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Mao, Chenhan] Nanjing Univ Chinese Med, Affiliated Hosp Integrated Tradit Chinese & Wester, Nanjing, Jiangsu, Peoples R China.
通讯机构：
[Xu, Y; Zhu, Y ] H;Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.
关键词：
Intestinal obstruction (IO);Global burden;Disability-adjusted life years (DALY);Joinpoint regression analysis;Age-period-cohort analysis
摘要：
BACKGROUND: Intestinal obstruction (IO) is a common surgical acute abdominal condition that places a significant burden on modern health systems. Unfortunately, the global burden and trends of IO remain unknown. Therefore, this study aimed to comprehensively assess its long-term trends and epidemiological features, which will help policymakers to formulate appropriate health policies. METHODS: We conducted an ecological study using data from the Global Burden of Disease Study (GBD) 2019. Data on IO were analyzed by sex, age, year, sociodemographic index (SDI), and location according to GBD 2019. In addition, joinpoint regression analysis was used to assess temporal trends. Age-period-cohort analysis (APC Analysis) was conducted to evaluate age, period, and birth cohort effects on IO incidence and mortality risk. RESULTS: Globally, the prevalent and incident cases increased by 56.91% and 86.67% from 1990 to 2019, respectively. Joinpoint regression analysis showed that age-standardized incidence rate (ASIR) and age-standardized prevalence rate (ASPR) increased, but age-standardized mortality rate (ASMR) and age-standardized disability-adjusted life year (DALY) rate decreased over the past three decades. The age effect demonstrated that older people have ahigher risk of morbidity and mortality. The period effect of incidence and mortality showed an upward trend from 1990 to 2019. Cohort effect revealed that the incidence and death risk peaked in the earlier-born cohort and was lower in the more recent-born cohort. Notably, we found that the burden of IO was higher in males than in females throughout the study period. There are huge disparities in IO burden among countries. CONCLUSION: Globally, the reported incidence and prevalence of IO increased from 1990 to 2019. The burden of IO differed markedly by age, sex, country, and region. Middle-aged and elderly people over 50 years old were at high risk. Given the ageing population, the burden of IO will be a major public health challenge. Thus, there is a strong necessity to strengthen prevention and early intervention in the at-risk population.
摘要：
Oncogenic super-enhancers (SEs) generate noncoding enhancer/SE RNAs (eRNAs/seRNAs) that exert a critical function in malignancy through powerful regulation of target gene expression. Herein, we show that a JUN-mediated seRNA can form R-loop to regulate target genes to promote metastasis of nasopharyngeal carcinoma (NPC). A combination of global run-on sequencing, chromatin-immunoprecipitation sequencing, and RNA sequencing was used to screen seRNAs. A specific seRNA associated with NPC metastasis (seRNA-NPCM) was identified as a transcriptional regulator for N-myc downstream-regulated gene 1 (NDRG1). JUN was found to regulate seRNA-NPCM through motif binding. seRNA-NPCM was elevated in NPC cancer tissues and highly metastatic cell lines, and promoted the metastasis of NPC cells in vitro and in vivo. Mechanistically, the 3' end of seRNA-NPCM hybridizes with the SE region to form an R-loop, and the middle segment of seRNA-NPCM binds to heterogeneous nuclear ribonucleoprotein R (hnRNPR) at the promoter of distal gene NDRG1 and neighboring gene tribbles pseudokinase 1 (TRIB1). These structures promote chromatin looping and long-distance chromatin interactions between SEs and promoters, thus facilitating NDRG1 and TRIB1 transcription. Furthermore, the clinical analyses showed that seRNA-NPCM and NDRG1 were independent prognostic factors for NPC patients. seRNA-NPCM plays a critical role in orchestrating target gene transcription to promote NPC metastasis.
期刊：
WORLD JOURNAL OF BIOLOGICAL PSYCHIATRY,2023年24(2):149-161 ISSN：1562-2975
通讯作者：
Deming Wang<&wdkj&>Ji Xiao
作者机构：
[Huang, Zixia; Wang, Deming; Xiao, Ji; Fan, Xuhong; Wu, Mingyue] Univ South China, Affiliated Hosp 2, Dept Anesthesiol, Hengyang, Peoples R China.;[Zhao, Zhenyu] Hunan Univ Chinese Med, Hosp 1, Dept Anesthesiol, Changsha, Peoples R China.
通讯机构：
[Deming Wang; Ji Xiao] D;Department of Anesthesiology, The Second Affiliated Hospital of University of South China, Hengyang, China<&wdkj&>Department of Anesthesiology, The Second Affiliated Hospital of University of South China, Hengyang, China
期刊：
Molecular and Cellular Biochemistry,2023年478(8):1791-1802 ISSN：0300-8177
通讯作者：
Qinghu He
作者机构：
[Luo, Min; Chen, Jisong; Hu, Zongren; He, Qinghu] Hunan Univ Med, Dept Rehabil & Healthcare, 492 Jinxi South Rd, Huaihua City, Hunan, Peoples R China.;[Wang, Neng; Hu, Zongren; He, Qinghu] Hunan Univ Chinese Med, Sch Integrated Chinese & Western Med, Changsha 410208, Hunan, Peoples R China.;[Zhang, Yuanting] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Luo, Min] Cent South Univ, Xiangya Hosp 2, Changsha 410011, Hunan, Peoples R China.;[Xiao, Yinfu] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Qinghu He] D;Department of Rehabilitation and Healthcare, Hunan University of Medicine, Huaihua City, China<&wdkj&>School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha, China
摘要：
Erectile dysfunction (ED) is a major health problem affecting a large proportion of the general population. Testosterone also plays a key role in sexual dysfunction. In this study, we found that testosterone can inhibit cavernous fibrosis by affecting the expression of miR-22-3p, providing a new basis for research and treatment of ED. Old and young rats were used to study the effects of testosterone on cavernous fibrosis. Hematoxylin and eosin (HE) and Masson's staining were used to observe the cavernous tissue. A luciferase assay was used to analyze the relationship between the miR-22-3p, TGF beta R1, and Galectin-1 signaling pathways. CCK-8 and flow cytometry were used to detect the proliferation and apoptosis rates of cavernosum smooth muscle cells (CSMCs) following testosterone intervention. Immunohistochemical analysis was performed to examine the positive rate of caspase 3 and Ki67. IF was used to analyze the expression of collagen IV, MMP2, and alpha-SMA. The levels of GnRH, tT, LH, and F-TESTO in old rats increased after testosterone intervention. miR-22-3p inhibits the expression of TGF beta R1 and Galectin-1. The protein expression of TGF beta R1, Galectin-1, SMAD2, and p-SMAD2 was reduced by testosterone. The expression levels of alpha-SMA, collagen I, collagen IV, FN, and MMP2 in the cavernous tissues of old rats treated with testosterone were significantly reduced. The levels of caspase 3 and collagen IV decreased, and the levels of MMP2, Ki67, and alpha-SMA increased. Testosterone and miR-22-3p inhibit CSMC apoptosis and promote cell proliferation. Testosterone promoted the expression of miR-22-3p to interfere with the expression of the cavernous TGF beta R1 and Galectin-1 signaling pathways. Testosterone can reduce cavernous fibrosis during the treatment of functional ED.
期刊：
Cell Biochemistry and Function,2023年41(7):857-867 ISSN：0263-6484
通讯作者：
Tang, CG;Wang, MQ
作者机构：
[Tang, Chenguang; Luo, Jing] Shenzhen Qianhai Shekou Free Trade Zone Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;[Deng, Yijue; Wang, MQ; Wang, Mengqing; Luo, Jing] Hunan Univ Chinese Med, Hosp 1, Dept Paediat, Changsha, Peoples R China.;[Ding, Yi] Changsha Social Work Coll, Sch Rehabil, Changsha, Peoples R China.
通讯机构：
[Tang, CG ] S;[Wang, MQ ] H;Shenzhen Qianhai Shekou Free Trade Zone Hosp, Dept Tradit Chinese Med, Shenzhen, Peoples R China.;Hunan Univ Chinese Med, Hosp 1, Dept Paediat, Changsha, Peoples R China.
关键词：
EKR signaling;Xie Bai Zeng Ye decoction;cytokine;montelukast sodium;postinfectious cough;respiratory function
摘要：
This study aimed to determine the effects of Xiebai Zengye decoction (XBZY)on airway inflammation and respiratory function in rats with postinfectious cough (PIC),and its regulatory effects on the extracellular signal-regulated kinase (ERK)signaling pathway. Compared with the normal group, the rats from thePIC group had significantly shortened expiratory time (TE)and enhanced pause (EEP),increased resistance (RT),and enhanced pause (Penh), along with increased levels of serum interleukin-4 (IL-4)and IL-6,and decreased levels of IL-10. The lung and colon tissues of rats from the PIC group showed histopathological changes, including inflammatory cell infiltration, damaged mucosal epithelium, and crypt structure, with significantly increased ERK mRNA and protein expression levels. Treatment with XBZY and montelukast sodium (MAS)improved the respiratory function and serum cytokine levels, reduced tissue inflammation, and decreased ERK mRNA and protein expression levels in the lung and colon tissues. In the lung tissues, XBZY treatment significantly decreased the expression of phosphorylated-ERK (p-ERK)protein, as well as p-MEK1/2, p-ERK1/2, and p-c-Fos proteins, while in the colon tissues, XBZY significantly decreased the expression of p-ERK1/2and p-c-Fos proteins. However, MAS treatment only showed significant improvement in the lung tissue inflammation score, and the expression level of p-ERK protein in the lung tissue was decreased. In conclusion, the present study suggests that XBZY has a potential therapeutic effect on PIC by improving respiratory function and attenuating inflammation, and this effect may be associated with the inhibition of the ERK signaling pathway. These findings could provide a new direction for the development of treatments for PIC. However, further research is needed to elucidate the underlying molecular mechanisms of XBZY and to confirm its safety and efficacy in clinical trials.
