摘要：
To investigate the anatomical features of frontal recess (FR) drainage, and the classification of FR cells and frontal sinus (FS). Fifty sides from 30 adult cadaver heads were examined. FR cells and FS along the drainage pathways were dissected under 0° and 70° endoscopic views using unique connecting structures between the uncinate process and the ethmoid bulla as landmarks. Connecting plates between the uncinate process and the ethmoid bulla were discovered and termed medial suprainfundibular plate (MSIP), which were observed on each cadaver head, and lateral suprainfundibular plate (LSIP) on 92% (46/50) sides. Separated by MSIP, two drainage pathways were identified and named medial pathways of the FR (MPFR) medial to the MSIP and the lateral pathways of the FR (LPFR) in the lateral side. Different drainage pathways of the FS were confirmed, in which drained into the MPFR in 37 and into the LPFR in 13 of the cadaver sides. MSIP is the critical landmark for the recognition of MPFR, LPFR, and the classification of FR cells. The FR resection along LPFR and MPFR facilitated excellent exposure of FS.
作者机构：
[王超; 谭成富; 刘薇薇; 杜琳; 唐雅妮; 陈美琳; 李姣兰] College of Acupuncture-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China;[章薇] The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007
作者机构：
[朱鼎铭; 王超; 谭成富; 刘薇薇; 杜琳; 唐雅妮; 郭礼娜; 陈美琳; 李姣兰] College of Acupuncture-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China;[章薇] Department of Acupuncture and Moxibustion of the First Affiliated Hospital, Hunan University of Chinese Medicine, Changsha 410007
作者机构：
[李迎红; 王世强; 廖小华] Physical Education College, Hunan University of Technology, Zhuzhou 412000, Hunan Province, China;[李敏] Department of Basic Medicine, Hunan College of Traditional Chinese Medicine, Zhuzhou 412000, Hunan Province;[李强] Department of Rehabi litation and Health Care, Hunan College of Traditional Chinese Medicine, Zhuzhou 412000, Hunan Province;[郭义] Center for Experimental Acupuncuture Research, Tianjin University of Traditional Chinese Medicine, Tianjin 300193;[Luo, Xiang-Jun] Department of Acupuncture and Moxibustion, The First Affiliated Hospital of Hunan College of Traditional Chinese Medicine, Zhuzhou 412000, Hunan Province
作者机构：
[吴云云; 邓多喜; 彭亮; 肖瑶; 张泓; 杨璧英; 黎帅; 黄桂兰; 谭洁; 郭奎奎] College of Acupuncture-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[谢辉] Department of Rehabilitation, Chenzhou First People's Hospital, Chenzhou 423000, Hunan Province, China
作者机构：
[吴云云; 邓多喜; 张泓; 刘家庆; 黎帅; 刘桐言; 黄桂兰; 谭洁; 郭奎奎] College of Acupuncture-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China
作者机构：
[刘丽; 魏歆然; 谢莉娜; 吴雪芬; 郭鑫; 岳增辉; 陈小丽; 郑雪娜; 谢志强] College of Acupuncture-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China
作者机构：
[王超; 谭成富; 刘薇薇; 杜琳; 宋瑾; 严洁; 唐雅妮; 冯果; 陈美琳; 阳晶晶; 李姣兰] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China
作者机构：
[谭静; 刘密; 刘迈兰; 马明珠; 邹逸凡; 石佳; 阳晶晶; 常小荣] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410208, China;[赵钊] Department of Acu-moxibustion and Tuina and Rehabilitation, the First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007
期刊：
Molecular Medicine Reports,2018年17(1):1173-1181 ISSN：1791-2997
通讯作者：
Zhou, Zhi-Wen;Tang, Qing-Ping
作者机构：
[Zhou, Zhi-Wen; Yang, Qi-Dong; Yang, Jie; Jiang, Wen; Guo, Rong-Jing] Cent S Univ, Dept Neurol, Xiangya Hosp, 87 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China.;[Tang, Qing-Ping] Cent S Univ, Dept Physiol, Sch Basic Med Sci, 90 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China.;[Tang, Qing-Ping] Hunan Univ Chinese Med, Brain Hosp Hunan Prov, Dept Rehabil, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Zhou, Zhi-Wen; Tang, Qing-Ping] C;Cent S Univ, Dept Neurol, Xiangya Hosp, 87 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China.;Cent S Univ, Dept Physiol, Sch Basic Med Sci, 90 Xiang Ya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
ERK/CREB pathway;Focal cerebral ischemia;GluR2/GRASP-l/PICKl cascades;Willed movement training
摘要：
Neurorehabilitation training is a therapeutic intervention for the loss of neural function induced by focal cerebral ischemia, however, the effect varies depending on the neurorehabilitation exercises. Willed movement (WM) training is defined as task-oriented training, which increases enthusiasm of patients to accomplish a specific task. The current study was performed to the evaluate effect of WM training on neurorehabilitation following focal cerebral ischemia, and further investigate the influence on neural plasticity-associated signaling pathway. Sprague-Dawley rats following temporary middle cerebral artery occlusion (tMCAO) were randomly divided into four groups: tMCAO (no rehabilitation training), CR (control rehabilitation), EM (environmental modification) and WM groups. Rats in the CR group were forced to exercise (running) in a rotating wheel. In the WM group, food was used to entice rats to climb on a herringbone ladder. Herringbone ladders were also put into the cages of the rats in the CR and EM groups, however without the food attraction. WM group exhibited an improvement in neurobehavioral performance compared with other groups. TTC staining indicated an evident reduction in brain damage in the WM group. There were increased synaptic junctions following WM training, based on the observations of transmission election microscopy. Investigation of the molecular mechanism suggested that WM training conferred the greatest effect on stimulating the extracellular signal-related kinase (ERK)/cyclic adenosine monophosphate response element-binding protein 1 (CREB) pathway and glutamate receptor 2 (GluR2)/glutamate receptor interacting protein 1-associated protein 1 (GRASP-l)/protein interacting with C-kinase 1 (PICK1) cascades among groups. Collectively, the improvement of neurobehavioral performance by WM training following tMCAO is suggested to involve the ERK/CREB pathway and GluR2/GRASP-l/PICKl cascades. The present study provided a preliminary foundation for future research on the therapeutic effect of WM training against stroke-induced neuron damage.
作者机构：
[郭斌; 谢涛; 胡岗珠; 刘迈兰; 杨俊; 谢莉娜; 吴雪芬; 岳增辉; 郑雪娜; 谢志强] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410208, China
作者机构：
[刘丽; 魏歆然; 吴雪芬; 郭鑫; 岳增辉; 陈小丽; 郑雪娜; 谢志强] College of Acupuncture-moxibustion and Massage,Hunan University of Chinese Medicine, Changsha 410007, China
通讯机构：
[Dong, JY; Yang, ZB] X;Xiamen Univ, Dept Elect Sci, Xiamen 361005, Peoples R China.;Xiamen Univ, Dept Tradit Chinese Med, Xiamen 361005, Peoples R China.
摘要：
Chronic atrophic gastritis (CAG) is a common gastrointestinal disease which has been considered as precancerous lesions of gastric carcinoma. Previously, electro-acupuncture stimulation has been shown to be effective in ameliorating symptoms of CAG. However the underlying mechanism of this beneficial treatment is yet to be established. In the present study, an integrated histopathological examination along with molecular biological assay, as well as 1H NMR analysis of multiple biological samples (urine, serum, stomach, cortex and medulla) were employed to systematically assess the pathology of CAG and therapeutic effect of electro-acupuncture stimulation at Sibai (ST 2), Liangmen (ST 21), and Zusanli (ST 36) acupoints located in the stomach meridian using a rat model of CAG. The current results showed that CAG caused comprehensive metabolic alterations including the TCA cycle, glycolysis, membrane metabolism and catabolism, gut microbiota-related metabolism. On the other hand, electro-acupuncture treatment was found able to normalize a number of CAG-induced metabolomics changes by alleviating membrane catabolism, restoring function of neurotransmitter in brain and partially reverse the CAG-induced perturbation in gut microbiota metabolism. These findings provided new insights into the biochemistry of CAG and mechanism of the therapeutic effect of electro-acupuncture stimulations.
摘要：
Acupuncture is a traditional Chinese medicine therapy that has been found useful for treating various diseases. The treatments involve the insertion of fine needles at acupoints along specific meridians (meridian specificity). This study aims to investigate the metabolic basis of meridian specificity using proton nuclear magnetic resonance ((1)H NMR)-based metabolomics. Electro-acupuncture (EA) stimulations were performed at acupoints of either Stomach Meridian of Foot-Yangming (SMFY) or Gallbladder Meridian of Foot-Shaoyang (GMFS) in healthy male Sprague Dawley (SD) rats. (1)H-NMR spectra datasets of serum, urine, cortex, and stomach tissue extracts from the rats were analysed by multivariate statistical analysis to investigate metabolic perturbations due to EA treatments at different meridians. EA treatment on either the SMFY or GMFS acupoints induced significant variations in 31 metabolites, e.g., amino acids, organic acids, choline esters and glucose. Moreover, a few meridian-specific metabolic changes were found for EA stimulations on the SMFY or GMFS acupoints. Our study demonstrated significant metabolic differences in response to EA stimulations on acupoints of SMFY and GMFS meridians. These results validate the hypothesis that meridian specificity in acupuncture is detectable in the metabolome and demonstrate the feasibility and effectiveness of a metabolomics approach in understanding the mechanism of acupuncture.
作者机构：
[Zeng, Fang; Lan, Lei; Liang, Fanrong; Li, Zhengjie; Guo, Taipin; Wu, Feng; Li, Ying; Yin, Tao; Gao, Yujie; Yang, Jie; Dong, Mingkai] Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.;[Jorgenson, Kristen; Kong, Jian; Makris, Nikos] Harvard Med Sch, Dept Psychiat, Massachusetts Gen Hosp, Boston, MA 02115 USA.;[Jorgenson, Kristen; Kong, Jian; Makris, Nikos] Massachusetts Gen Hosp, Martinos Ctr Biomed Imaging, Charlestown, MA 02129 USA.;[Liu, Mailan] Hunan Univ Chinese Med, Acupuncture & Tuina Sch, Changsha, Hunan, Peoples R China.;[Gong, Qiyong] Sichuan Univ, West China Hosp, Huaxi MR Res Ctr, Chengdu, Sichuan, Peoples R China.
通讯机构：
[Liang, Fanrong] C;[Kong, Jian] H;[Kong, Jian] M;Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.;Harvard Med Sch, Dept Psychiat, Massachusetts Gen Hosp, Boston, MA 02115 USA.
关键词：
Migraine;Amplitude of low-frequency fluctuations;Brainsterm;Trigeminocervical complex;Acupuncture;Resting state fMRI
摘要：
Migraine is a common neurological disease with a high prevalence and unsatisfactory treatment options. The specific pathophysiological mechanisms of migraine remain unclear, which restricts the development of effective treatments for this prevalent disorder. The aims of this study were to 1) compare the spontaneous brain activity differences between Migraine without Aura (MwoA) patients and healthy controls (HCs), using amplitude of low-frequency fluctuations (ALFF) calculation method, and 2) explore how an effective treatment (verum acupuncture) could modulate the ALFF of MwoA patients. One hundred MwoA patients and forty-six matched HCs were recruited. Patients were randomized to four weeks' verum acupuncture, sham acupuncture, and waiting list groups. Patients had resting state BOLD-fMRI scan before and after treatment, while HCs only had resting state BOLD-fMRI scan at baseline. Headache intensity, headache frequency, self-rating anxiety and self-rating depression were used for clinical efficacy evaluation. Compared with HCs, MwoA patients showed increased ALFF in posterior insula and putamen/caudate, and reduced ALFF in rostral ventromedial medulla (RVM)/trigeminocervical complex (TCC). After longitudinal verum acupuncture treatment, the decreased ALFF of the RVM/TCC was normalized in migraine patients. Verum acupuncture and sham acupuncture have different modulation effects on ALFF of RVM/TCC in migraine patients. Our results suggest that impairment of the homeostasis of the trigeminovascular nociceptive pathway is involved in the neural pathophysiology of migraines. Effective treatments, such as verum acupuncture, could help to restore this imbalance.
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Chinese Medicine, Changsha 410007, China;[林亚平; 彭艳; 岳增辉] Key Acu-moxibustion Laboratory of Biological Information Analysis of Institute of Acupuncture, Moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China
作者机构：
[Wang, J. Y.; Duanmu, C. L.; Liu, J. L.; Gao, Y. H.] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Dept Physiol, Beijing 100700, Peoples R China.;[Li, C. W.; Chang, X. R.] Hunan Univ Chinese Med, Coll Acupuncture & Moxibust & Tui Na, Changsha 410208, Hunan, Peoples R China.;[Li, C. W.] Hunan Tradit Chinese Med Coll, Affiliated Hosp 1, Zhuzhou 412012, Hunan, Peoples R China.
通讯机构：
[Liu, J. L.] C;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Dept Physiol, Beijing 100700, Peoples R China.
摘要：
Increasing evidence supports that acupuncture intervention is an effective approach for intraoperative and postoperative pain. Neuron-microglia crosstalk, mediated by the purinergic P2X7 receptor (R)/fractalkine/CX3CR1 cascade in the spinal cord dorsal horn, plays a pivotal role in pain processing. However, its involvement in the analgesic effect of electroacupuncture (EA) remains unclear. In this study, a rat neck-incision pain model was established by making a longitudinal incision along the midline of the neck and subsequent repeated mechanical stimulation. EA stimulation was applied to bilateral LI18, LI4-PC6, or ST36-GB34. The thermal pain threshold, cervicospinal ATP concentration, expression levels of purinergic P2XR and P2YR subunits mRNAs, and fractalkine, CX3CR1 and p38 MAPK proteins, were detected separately. The neck incision induced strong thermal hyperalgesia and upregulation of spinal ATP within 48 h. No significant change was found in thermal hyperalgesia after a single session of EA intervention. However, a single session of EA dramatically enhanced the neck incision-induced upregulation of ATP and upregulated the expression of P2X7R, which was reversed by two sessions of EA. Two sessions of EA at bilateral LI18 or LI4-PC6 attenuated hyperalgesia significantly, accompanied with downregulation of P2X7R/fractalkine/ CX3CR1 signaling after three sessions of EA. EA stimulation of LI18 or LI4-PC6 alleviates thermal hyperalgesia in neck-incision pain rats, which may be associated with its effects in regulating the neck incision-induced increase of ATP and P2X7R and subsequently suppressing fractalkine/CX3CR1 signaling in the cervical spinal cord.
