作者机构：
[魏歆然] The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[魏高文] College of Integrated Traditional Chinese and Western Medicine, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[文琪琦; 刘丽; 谢莉娜; 吴雪芬; 陈小丽; 郑雪娜; 谢志强] College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[岳增辉] College of Acupuncture and Moxibustion, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. 624755064@qq.com
作者机构：
[Zeng, Fang; Lan, Lei; Liang, Fanrong; Li, Zhengjie; Guo, Taipin; Wu, Feng; Li, Ying; Gao, Yujie; Yang, Jie; Dong, Mingkai] Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.;[Li, Zhengjie] Xiamen Univ, Dept Tradit Chinese Med, Coll Med, Xiamen, Fujian, Peoples R China.;[Sun, Sharon; Kong, Jian; Makris, Nikos] Massachusetts Gen Hosp, Dept Psychiat, Boston, MA 02114 USA.;[Sun, Sharon; Kong, Jian; Makris, Nikos] Harvard Med Sch, Boston, MA USA.;[Sun, Sharon; Kong, Jian; Makris, Nikos] Massachusetts Gen Hosp, Martinos Ctr Biomed Imaging, Charlestown, MA USA.
通讯机构：
[Liang, Fanrong] C;Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.
关键词：
Acupuncture;migraine without aura;fMRI;independent component analysis;frontoparietal network;resting-state functional connectivity
摘要：
Aims: This study aims to investigate the resting-state functional connectivity (rs-fc) of the right frontoparietal network (rFPN) between migraineurs and healthy controls (HCs) in order to determine how the rFPN rs-fc can be modulated by effective treatment. Methods: One hundred patients and 46 matched HCs were recruited. Migraineurs were randomized to verum acupuncture, sham acupuncture, and waiting list groups. Resting-state functional magnetic resonance imaging data were collected before and after longitudinal treatments. Independent component analysis was applied in the data analysis. Results: We found that migraineurs showed decreased rs-fc between the rFPN and bilateral precuneus compared with HCs. After treatments (real and sham), rFPN rs-fc with the precuneus was significantly reduced. This reduction was associated with headache intensity relief. In order to explore the role of the precuneus in acupuncture modulation, we performed a seed-based rs-fc analysis using the precuneus as a seed and found that the precuneus rs-fc with the bilateral rostral anterior cingulate cortex/medial prefrontal cortex, ventral striatum, and dorsolateral prefrontal cortex was significantly enhanced after treatment. Conclusion: Our results suggest that migraineurs are associated with abnormal rFPN rs-fc. An effective treatment, such as acupuncture, may relieve symptoms by strengthening the cognitive adaptation/coping process. Elucidation of the adaptation/coping mechanisms may open up a new window for migraine management.
作者机构：
[彭艳; 刘丽; 刘密; 吴雪芬; 郭鑫; 岳增辉; 郑雪娜] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China;[袁建菱] College of Acupuncture and Moxibustion, Hunan University of Chinese Medicine, Changsha 410007, China. 624755064@qq.com
作者机构：
[谢文娟; 刘密; 林海波; 谭成富; 贺香嫦; 常小荣; 阳晶晶] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China;[严洁] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. yj5381159@126.com;[王超] College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410007, China. 592436380@qq.com
作者机构：
[杨建文; 刘丽; 刘薇薇; 陈海交; 张程程] Graduate School, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[彭艳; 刘密] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;[林亚平] Key Lab for Acu-moxibustion Biological Information Analysis of College of Acu-moxibustion and Massage, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. lyp5381161@126.com
作者机构：
[Zeng, Fang; Lan, Lei; Liang, Fanrong; Zeng, F; Li, Zhengjie; Guo, Taipin; Wu, Feng; Li, Ying; Gao, Yujie; Yang, Jie; Dong, Mingkai] Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.;[Li, Zhengjie] Xiamen Univ, Coll Med, Dept Tradit Chinese Med, Xiamen, Fujian, Peoples R China.;[Liu, Mailan] Hunan Univ Chinese Med, Acupuncture & Tuina Sch, Changsha, Hunan, Peoples R China.;[Kong, Jian; Makris, Nikos] Massachusetts Gen Hosp, Dept Psychiat, Charlestown, MA USA.;[Kong, Jian; Makris, Nikos] Harvard Univ, Sch Med, Charlestown, MA USA.
通讯机构：
[Zeng, F; Liang, FR] C;Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.
摘要：
The aims of this study were to 1) compare resting state functional connectivity (rs-fc) of the periaqueductal gray (PAG), a key region in the descending pain modulatory system (DPMS) between migraine without aura (MwoA) patients and healthy controls (HC), and 2) investigate how an effective treatment can influence the PAG rs-fc in MwoA patients. One hundred MwoA patients and forty-six matched HC were recruited. Patients were randomized to verum acupuncture, sham acupuncture, and waiting list groups. Resting state fMRI data were collected and seed based functional connectivity analysis was applied. Compared with HC, MwoA patients showed reduced rs-fc between the PAG and rostral anterior cingulate cortex/medial prefrontal cortex (rACC/mPFC), key regions in the DPMS and other pain related brain regions. The reduced rs-fc between the PAG and rACC/mPFC was associated with increased migraine headache intensity at the baseline. After treatments, rs-fc between the PAG and the rACC in MwoA patients significantly increased. The changes of rs-fc among the PAG, rACC and ventral striatum were significantly associated with headache intensity improvement. Impairment of the DPMS is involved in the neural pathophysiology of migraines. Impaired DPMS in migraine patients can be normalized after effective treatment.
关键词：
Moxibustion;Point ST 21 (Liangmen);Point ST 36 (Zusanli);Stomach ulcer;Apoptosis;Cell proliferation
摘要：
OBJECTIVE: To observe the effect of moxa-burning heat stimulating acupoints of Liangmen (ST 21) and Zusanli (ST 36) on the proliferation and apoptosis signaling proteins in rats with stress-induced gastric ulcer. METHODS: Forty rats were randomly divided into four groups: negative control (NC), ulcer control (UC), acupoints of stomach meridian (ASM), and acupoints control (AC). The acute gastric ulcer model was established by bound and water immersion. Rats in NC and UC groups didn't receive any moxa-burning heat stimulating treatment, while rats in ASM and AC groups were treated with buringmoxa heat stimulating the acupoints of Liangmen (ST 21) and Zusanli (ST 36) and their controlled points, respectively. Rats in all groups were sacrificed after 12 consecutive days treatment. The ulcer index was evaluated by using Guth's method. The expression of tumor necrosis factor-alpha (TNF-α), apoptotic protease activating facter-1 (Apaf-1), Caspase-3, p21 activated kinase 1 (PAK1), extracellular regulated protein kinases 2 (ERK2), phosphorylated ERK2 (pERK2), phosphoinositide 3-kinase (PI3K) and RAC-alpha serine/threonine- protein kinase (Akt) in gastric mucosa was detected by enzyme linked immunosorbent assay (ELISA). RESULTS: Compared with UC group, the ulcer index of ASM and AC groups decreased, and the injured gastric mucosa was improved, the expression of TNF-α, Apaf-1 and Caspase-3 in gastric mucosa was significantly reduced (P < 0.05), while the expression of PAK1, ERK2, pERK2, PI3K and Akt in gastric mucosa was significantly increased (P < 0.05). And ASM showed better effect than AC group (P < 0.05). CONCLUSION: Moxa-burning Heat stimulating of Liangmen (ST 21) and Zusanli (ST 36) could promote the recovery of gastric mucosal lesion probably by inhibiting cell apoptosis and promoting cell proliferation in stress-induced gastric ulcer.
作者机构：
[Tang, Qing-ping] Hunan Univ Chinese Med, Brain Hosp Hunan Prov, Dept Rehabil, Changsha 410007, Hunan, Peoples R China.;[Tang, Qing-ping; Wu, Li-xiang] Cent S Univ, Sch Basic Med Sci, Dept Physiol, Changsha 410078, Hunan, Peoples R China.;[Shen, Qin] Cent S Univ, Xiangya Hosp, Dept Neurol, Changsha 410008, Hunan, Peoples R China.;[Feng, Xiang-ling; Liu, Hui] Cent S Univ, Xiangya Sch Med, Canc Res Inst, Changsha 410078, Hunan, Peoples R China.;[Wu, Bei] Cent S Univ, Xiangya Hosp, Dept Otolaryngol, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Liu, Zun-jing] C;China Japan Friendship Hosp, Dept Neurol, Beijing 100029, Peoples R China.
关键词：
Motor training;Signal transducer and activator of transcription 3 (STAT3);Brain-derived neurotrophic factor (BDNF);Protein interacting with C kinase 1 (PICK1);Neural plasticity
摘要：
Willed-movement training has been demonstrated to be a promising approach to increase motor performance and neural plasticity in ischemic rats. However, little is known regarding the molecular signals that are involved in neural plasticity following willed-movement training. To investigate the potential signals related to neural plasticity following willed-movement training, littermate rats were randomly assigned into three groups: middle cerebral artery occlusion, environmental modification, and willed-movement training. The infarct volume was measured 18 d after occlusion of the right middle cerebral artery. Reverse transcription-polymerase chain reaction (PCR) and immunofluorescence staining were used to detect the changes in the signal transducer and activator of transcription 3 (STAT3) mRNA and protein, respectively. A chromatin immunoprecipitation was used to investigate whether STAT3 bound to plasticity-related genes, such as brain-derived neurotrophic factor (BDNF), synaptophysin, and protein interacting with C kinase 1 (PICK1). In this study, we demonstrated that STAT3 mRNA and protein were markedly increased following 15-d willed-movement training in the ischemic hemispheres of the treated rats. STAT3 bound to BDNF, PICK1, and synaptophysin promoters in the neocortical cells of rats. These data suggest that the increased STAT3 levels after willed-movement training might play critical roles in the neural plasticity by directly regulating plasticity-related genes.
作者机构：
[陈伟; 李成文] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China;[端木程琳; 陈淑萍; 王俊英] Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700;[常小荣] College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha 410007, China. xrchang1956@163.com;[刘俊岭] Department of Physiology, Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700. 13521898023@163.com
摘要：
Background To investigate the similarities and differences in cerebral responses to puncturing at different acupoints for treating meal-related functional dyspepsia (FD). Methods Twenty right-handed FD patients were enrolled and randomized divided into two groups. Each patient received 20 sessions' electroacupuncture treatment. The acupoints used in Group A were four acupoints on the Stomach Meridian, and the acupoints used in Group B were four acupoints on the Gallbladder Meridian. PET-CT scans were performed before and after acupuncture treatment to record the changes of cerebral glycometabolism. Key Results After treatment, the dyspepsia symptoms and the quality of life (QOL) of the patients in each group were significantly improved (p < 0.05) and there was insignificant difference in efficacy between the two groups (p > 0.05). In Group A, deactivation in brainstem, bilateral anterior cingulate cortex (ACC) and cerebellum, left superior medial frontal gyrus, orbital frontal cortex (OFC), and thalamus, etc., and activation in bilateral middle cingulate cortex (MCC), precuneus and lingual gyrus, etc. were observed. In Group B, deactivation in brainstem, bilateral thalamus, putamen, ACC, postterior cingulate cortex, hippocampus and cerebellum, etc., and activation in bilateral MCC, precuneus, left OFC, etc. were observed (p < 0.05, Family-wise error corrected). Conclusions & Inferences Different acupoints have similar clinical efficacy but relatively different cerebral responses. The influence on the sensory transduction regions (brainstem and thalamus) and visceral modulation regions might be the common mechanism of different acupoints treating for FD, and the modulation on some emotion/cognition-related areas (e.g., prefrontal cortex) is the potential difference between the different acupoints.
摘要：
OBJECTIVE: To design a combined dynamic inhalation device for testing the toxicity induced by moxa smoking.METHODS: The new apparatus (Patent No.201120101911.5) includes air renewal and recycling systems, a gas generating device, a gas control unit, and a device to measure and control temperature and humidity. Sprague-dawley rats were tested for acute and sub-chronic toxicity after exposure to moxa-burning smoke.RESULTS: We found an LC_(50) of 1.2 × 10~4 mg/m~3 in the acute toxicity assays. In sub-chronic toxicity tests the organ coefficients studied showed no significant differences within rats groups of the same gender after treatment with moxa smoke or a month of recovery. However, mean gray degree of lung 70 heat shock protein (HSP70) was significantly elevated in the high dose group in comparison with the low dose group (P < 0.05); mean gray degree, mean optical density, gross area of HSP70 in other organs and caspase-9 parameters showed no significant differences between groups.CONCLUSION: These results suggest that moxa smoke had no overt toxicity in rats. This work provides evidence and reference for the design of dynamic inhalation exposure systems.
作者机构：
[Wu Xiaqiu; Peng Jin] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.;[Li Guoqin] China Acad Chinese Med Sci, Guanganmen Hosp, Dept Resp, Beijing 100700, Peoples R China.;[Zhang Wei] Hunan Univ Chinese Med, Hosp 1, Dept Acupuncture & Massage, Changsha 410000, Hunan, Peoples R China.;[Liu Guangxia] Anhui Univ Chinese Med, Hosp 2, Dept Acupuncture, Hefei 230000, Peoples R China.;[Liu Baoyan] China Acad Chinese Med Sci, Beijing 100700, Peoples R China.
通讯机构：
[Peng Jin] C;China Acad Chinese Med Sci, Inst Basic Res Clin Med, Dept Prevent Med, Beijing 100700, Peoples R China.
关键词：
Acupoint sticking therapy;Quality of Life;Self efficacy;Asthma;Chinese medicine, traditional
摘要：
OBJECTIVE: To evaluate the self-efficacy and satisfaction of asthma patients subjected to summer acupoint application treatment (SAAT). METHODS: A two-year follow-up clinical study was conducted. Patients with asthma were treated by applying a herbal paste onto the Feishu (BL 13) and Fengmen (BL 12) acupoints on the three hottest days of summer, according to the traditional Chinese calendar, from 2008 to 2010. During a two-year follow-up, these patients were asked to fill out a questionnaire evaluating asthma degree, exacerbation frequency, concomitant medications and self-satisfaction. The self-rate and doctor-report outcomes obtained in parallel were evaluated to assess the efficacy of SAAT. RESULTS: A total of 527 asthma patients were initially enrolled in this study, of which 97 elderly patients and those with more severe cases of asthma were lost to follow-up. Thus, a total of 430 patients were valid for analysis using self-rate data. Nevertheless, occasional negative returns were obtained; almost all of the outcomes were rated as "No change", "Moderate effective", or "Very effective". In addition, 80% of the patients were satisfied with this treatment. Moreover, 391 (91.4%) patients were somewhat improved after SAAT in 2009, and further improvement was observed in 2010. After SAAT, the average asthma-degree score decreased from 5.3 in 2008 to 4 in 2009 and, subsequently to 3.5 in 2010. CONCLUSION: With pronounced patient satisfaction, SAAT can reduce the exacerbation severity and frequency, concomitant medications and asthma degree. Prolonging the treatment course might enhance the efficacy of SAAT. (C) 2015 JTCM. All rights reserved.
期刊：
Journal of Acupuncture and Meridian Studies,2013年6(2):82-88 ISSN：2005-2901
通讯作者：
Jie Yan
作者机构：
[He, Jun-Feng; Qu, Ya-Ting] College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China;[Yan, Jie; Li, Jiang-Shan; Wang, Chao; Chang, Xiao-Rong] College of Acu-Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha, China;[Liu, Jian-Hua] Key Laboratory of Rule and Mechanism on Acupuncture, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China
通讯机构：
[Jie Yan] C;College of Acu-Moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha, China
