摘要：
Background: Nasopharyngeal carcinoma (NPC) is a usual head and neck malignancy. Guggulsterone (GS) has potential in cancer chemoprophylaxis and treatment, but its therapeutic effect on NPC is unknown. We aimed to explore whether GS could promote the secretion of exosomal circFIP1L1 from NPC cells and its regulatory mechanism.<&wdkj&>Methods: NPC tissues and adjacent tissues were collected from NPC patients. Human nasopharyngeal epithelial cell lines (NP69) and NPC lines (5-8F, CNE1, and HNE1) were used for in vitro experiments. HNE1 cells were treated with GS (20, 40, 60 μmol/L). The expressions of miR-125a-5p and circFIP1L1 were evaluated by qRT-PCR. Cell proliferation and apoptosis abilities were measured by CCK-8 and flow cytometry. HNE1 cell exosomes were extracted and identified, and the levels of VEGFA and VEGFR2 were detected by ELISA. Then miR-125a-5p was knocked down and overexpressed. HUVECs angiogenesis was determined by the tube formation assay. qRT-PCR and Western blot were utilized to evaluate the expressions of VEGFA, MMP-2, MMP-9, and ICAM-1 in HUVECs.<&wdkj&>Results: miR-125a-5p was highly expressed in NPC tissues and cells. GS promoted the secretion of exosomal circFIP1L1 from HNE1 cells to affect HUVECs proliferation and angiogenesis. Overexpression of miR-125a-5p accelerated HUVECs proliferation and angiogenesis. Knocking down miR-125a- 5p inhibited VEGFA expression. In addition, exosomal circFIP1L1 sponged miR-125a-5p, inhibiting the VEGFA pathway to repress HUVECs angiogenesis.<&wdkj&>Conclusions: GS promoted exosomal circFIP1L1 in NPC cells to mediate miR-125a-5p/VEGFA axis affecting tumor angiogenesis.
作者机构：
[Chen, Xi; Liu, Ying; Cao, Hui; Qiu, Huiwen; Xu, Caijuan; Li, Sixin; Li, Xinyu; Tan, Rongrong] Hunan Univ Chinese Med, Sch Clin Med, Dept Psychiat, Changsha, Hunan, Peoples R China.;[Chen, Xi; Liu, Ying; Cao, Hui; Qiu, Huiwen; Xu, Caijuan; Li, Sixin; Li, Xinyu; Tan, Rongrong] Brain Hosp Hunan Prov, Peoples Hosp Hunan Prov 2, Dept Psychiat, Changsha, Hunan, Peoples R China.;[Huang, Wei] Cent South Univ, Xiangya Hosp, Dept Integrated Tradit Chinese & Western Med, Changsha, Hunan, Peoples R China.;[Cheng, Quan] Cent South Univ, Xiangya Hosp, Dept Neurosurg, Changsha, Hunan, Peoples R China.;[Cheng, Quan] Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha, Hunan, Peoples R China.
通讯机构：
[Hui Cao; Quan Cheng] D;Department of Psychiatry, The School of Clinical Medicine, Hunan University of Chinese Medicine, Changsha, Hunan, China<&wdkj&>Department of Psychiatry, Brain Hospital of Hunan Province (The Second People’s Hospital of Hunan Province), Changsha, Hunan, China<&wdkj&>Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan, China<&wdkj&>National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Hunan, China
摘要：
Disorders of central nervous system (CNS) disorders are considered serious health issues. The most common CNS diseases include neurodegenerative diseases, mental disorders, demyelinating disease, ischemia-reperfusion injury, and neuroinflammation. As a natural phenolic compound, hesperidin is a flavanone glycoside with various biological effects. Increasing evidence show that the growth of CNS diseases is hindered by hesperidin. Here, we have reviewed the related literature on neuropharmacological mechanisms for the preventive and therapeutic effects of hesperidin on CNS diseases. Several cellular and animal models have been developed to evaluate the underlying neuropharmacological mechanisms of hesperidin. Additionally, clinical evidence has confirmed its neuroprotective function. Hesperidin exerts its neuroprotective properties by decreasing neuro-inflammatory and apoptotic pathways. Hesperidin function has been studied in preclinical models for CNS diseases, but little is known about its definite effect in humans. Hesperidin can effectively alleviate depression and improve cognition and memory. It is urgent to explore and discover clinical trials for further confirmation of the neuroprotective efficacy of hesperidin and to evaluate its safety profile.
作者机构：
[Wang, Xiaofan; Wu, Hongzheng; Chen, Zhenni; Peng, Huanqie; Chen, Wanxin; Wang, Bingqi; Wang, Min] Cent South Univ, Dept Lab Med, Second Xiangya Hosp, Changsha 410011, Hunan, Peoples R China.;[Huang, Yiran] Hunan Univ Chinese Med, Sch Clin Med, Changsha 410208, Hunan, Peoples R China.
通讯机构：
[Wang, M ] C;Cent South Univ, Dept Lab Med, Second Xiangya Hosp, Changsha 410011, Hunan, Peoples R China.
摘要：
Bipolar disorder (BD) is a distinctly heterogeneous and multifactorial disorder with a high individual and social burden. Immune pathway dysregulation is an important pathophysiological feature of BD. Recent studies have suggested a potential role for T lymphocytes in the pathogenesis of BD. Therefore, greater insight into T lymphocytes' functioning in patients with BD is essential. In this narrative review, we describe the presence of an imbalance in the ratio and altered function of T lymphocyte subsets in BD patients, mainly in T helper (Th) 1, Th2, Th17 cells and regulatory T cells, and alterations in hormones, intracellular signaling, and microbiomes may be potential causes. Abnormal T cell presence explains the elevated rates of comorbid inflammatory illnesses in the BD population. We also update the findings on T cell-targeting drugs as potentially immunomodulatory therapeutic agents for BD disease in addition to classical mood stabilizers (lithium, valproic acid). In conclusion, an imbalance in T lymphocyte subpopulation ratios and altered function may be involved in the development of BD, and maintaining T cell immune homeostasis may provide an overall therapeutic benefit.
期刊：
Journal of Clinical Nursing,2023年32(13-14):3504-3515 ISSN：0962-1067
通讯作者：
Zhang, YH
作者机构：
[Yu, Qian; Feng, Xiaolin; Zhang, Yinhua; Pu, Haixu; Yan, Lichun] Hunan Univ Chinese Med, Sch Nursing, 300,Xueshi Rd, Changsha 410208, Peoples R China.;[Zhang, Xiaoqin] Hunan Univ Chinese Med, Sch Marxism, Changsha, Peoples R China.;[Luo, Liangchu] Hunan Univ Chinese Med, Sch Clin Med, Changsha, Peoples R China.
通讯机构：
[Zhang, YH ] H;Hunan Univ Chinese Med, Sch Nursing, 300,Xueshi Rd, Changsha 410208, Peoples R China.
关键词：
aged care facilities;associated risk factors;physical restraints;prevalence
摘要：
Aims and Objectives To investigate the use of physical restraints in aged care facilities(ACFs)and analyse its associated risk factors. Background Physical restraints have been widely used in ACFs worldwide, but they can cause physical and mental harm to older people. It is important to regulate the use of physical restraint. Design A cross-sectional observational and correlational multicentre study. Methods By convenience sampling method, we selected eight ACFs in four representative regions of Hunan province, China, for this study. The ACF-related information was obtained by interviewing the managers and reviewing records. We conducted investigation and observation on the elderly in the ACFs to understand the use of physical restraints at three different times: 9:30-11:30, 16:00-18:00 and 19:30-21:30 on a working day. The STROBE checklist was followed for this cross-sectional study. Results This study found that the utilisation rate of physical restraints was 23.2%. The critical risk factors affecting the use of physical restrains include the following: (1) the ratio of nursing staff to the elderly residents; (2)whether there is a dementia care unit at the facility; (3) the number of elderly residents in each room; (4) the elderly residents' age, degree of education, marital status, care dependence and cognitive impairment; (5) whether the elderly has suffered from a stroke or senile dementia; (6) whether the elderly carries medical catheters. Conclusion There is a lack of standardisation in the use of physical restraints in ACFs of central China. Chinese ACFs should develop guidelines and reduction measures to standardise the use of physical restraints, basing on the key factors affecting the use of physical restraints. Relevance to clinical practice The use of physical restraints in ACFs is threatening the safety of the elderly residents. Understanding the implementation of physical restraint in ACFs can provide reference for reducing the use of physical restraint.
作者机构：
[Xia, Zi-An] Cent South Univ, Xiangya Hosp, Dept Integrated Tradit Chinese & Western Med, Changsha 410008, Peoples R China.;[Xia, Zi-An; He, Jiang; Sun, Lunquan] Cent South Univ, Natl Clin Res Ctr Geriatr Disorders, XiangyaHosp, Changsha 410008, Peoples R China.;[Lu, Can] Cent South Univ, Xiangya Hosp, Dept Pathol, Changsha 410078, Peoples R China.;[Pan, Can] Hunan Univ Tradit Chinese Med, Sch Clin Med, Changsha 410208, Peoples R China.;[Li, Jia] Cent South Univ, Xiangya Hosp, Dept Emergency, Changsha 410008, Peoples R China.
通讯机构：
[Sun, LQ ; He, J ; Sun, LQ] ;Cent South Univ, Natl Clin Res Ctr Geriatr Disorders, XiangyaHosp, Changsha 410008, Peoples R China.;Cent South Univ, Xiangya Canc Ctr, Dept Oncol, XiangyaHosp, Changsha 410008, Peoples R China.;Key Lab Mol Radiat Oncol Hunan Prov, Changsha 410008, Peoples R China.;Hunan Int Sci & Technol Collaborat Base Precis Med, Changsha 410008, Peoples R China.
关键词：
Large-scale data analysis;Tumour-infiltrating lymphocytes;CD103;LAG3;Immunotherapy;Chemotherapy
摘要：
Tumour-infiltrating lymphocytes (TILs), including T and B cells, have been demonstrated to be associated with tumour progression. However, the different subpopulations of TILs and their roles in breast cancer remain poorly understood. Large-scale analysis using multiomics data could uncover potential mechanisms and provide promising biomarkers for predicting immunotherapy response. Single-cell transcriptome data for breast cancer samples were analysed to identify unique TIL subsets. Based on the expression profiles of marker genes in these subsets, a TIL-related prognostic model was developed by univariate and multivariate Cox analyses and LASSO regression for the TCGA training cohort containing 1089 breast cancer patients. Multiplex immunohistochemistry was used to confirm the presence of TIL subsets in breast cancer samples. The model was validated with a large-scale transcriptomic dataset for 3619 breast cancer patients, including the METABRIC cohort, six chemotherapy transcriptomic cohorts, and two immunotherapy transcriptomic cohorts. We identified two TIL subsets with high expression of CD103 and LAG3 (CD103+LAG3+), including a CD8+ T-cell subset and a B-cell subset. Based on the expression profiles of marker genes in these two subpopulations, we further developed a CD103+LAG3+ TIL-related prognostic model (CLTRP) based on CXCL13 and BIRC3 genes for predicting the prognosis of breast cancer patients. CLTRP-low patients had a better prognosis than CLTRP-high patients. The comprehensive results showed that a low CLTRP score was associated with a high TP53 mutation rate, high infiltration of CD8 T cells, helper T cells, and CD4 T cells, high sensitivity to chemotherapeutic drugs, and a good response to immunotherapy. In contrast, a high CLTRP score was correlated with a low TP53 mutation rate, high infiltration of M0 and M2 macrophages, low sensitivity to chemotherapeutic drugs, and a poor response to immunotherapy. Our present study showed that the CLTRP score is a promising biomarker for distinguishing prognosis, drug sensitivity, molecular and immune characteristics, and immunotherapy outcomes in breast cancer patients. The CLTRP could serve as a valuable tool for clinical decision making regarding immunotherapy.
