摘要：
目的:探讨影响冠状动脉粥样硬化性心肌病(简称冠心病)血瘀证发生的相关危险因素.方法:选择2016年1月至2018年5月于海南省中医院心血管内科就诊的292例冠心病患者为研究对象,根据是否为血瘀证分为冠心病血瘀证组175例与非血瘀证组117例.选择可能影响冠心病血瘀证的临床指标进行单因素分析及多因素非条件logistic回归方法.结果:单因素分析显示,年龄、体质指数(body mass index,BMI)、高压病史、糖尿病史、吸烟史、食用油消耗、食盐消耗、睡眠状况、体育锻炼、情绪问题及实验室指标总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG),高密度脂蛋白(high density lipoprotein choles-terol,HDL-C)、低密度脂蛋白(low-density lipoprotein cholesterol,LDL-C)脂蛋白相关磷脂酶A2(Lp-PLA2)与冠心病血瘀证密切相关(P<0.05).多因素Logistic回归模型发现影响冠心病血瘀证的独立危险因素有高血压病史(OR=2.221)、糖尿病史(OR=3.159)、食用油消耗(OR=1.551)、食盐消耗(OR=1.738)、体育锻炼(OR=0.471)、情绪问题(OR=1.388)、Lp-PLA2(OR=2.030),其中体育锻炼为保护性因素.结论:高压病史、糖尿病史、食用油消耗、食盐消耗、体育锻炼、情绪问题、Lp-PLA2是影响冠心病血瘀证的独立危险因素,其中体育锻炼为保护性因素.
摘要：
<jats:p> Astragalus and Panax notoginseng are commonly used to treat cardio-cerebrovascular diseases in China and are often combined together to promote curative effect. We speculate that the enhancement of the combination on anticerebral ischemia injury may come from the main active components. The purpose of this work was to probe the effects and mechanisms of Astragaloside IV (the active component of Astragalus) combined with Ginsenoside Rg1, Ginsenoside Rb1, and Notoginsenoside R1 (the active components of P. notoginseng) to antagonize ischemia/reperfusion (I/R) injury via inflammation and apoptosis. C57BL/6 mice were randomly divided into sham, model, Astragaloside IV, Ginsenoside Rg1, Ginsenoside Rb1, Notoginsenoside R1, four active components combination, and Edaravone groups. After administration for 3 days, bilateral common carotid arteries (CCA) were occluded with artery clip for 20[Formula: see text]min followed by reperfusion for 24[Formula: see text]h. Our results showed that the survival rate of nerve cell in hippocampal CA1 decreased while the apoptotic rate increased, and the level of caspase-3 protein in brain tissues was elevated, the expressions of TNF-a, IL-1, and ICAM-1 mRNA as well as phosphorylated nuclear factor kappa B (NF-[Formula: see text]B) inhibitor protein [Formula: see text] (p-I[Formula: see text]Ba) in brain tissues were up-regulated, and the nuclear translocation rate of NF-[Formula: see text]B was raised. Additionally, the protein expressions of phosphorylated tyrosine kinase 1 (p-JAK1), phosphorylated signal transducer and activator of transcription-1 (p-STAT1), glucose regulated protein 78 (GRP78), caspase-12, and phosphorylated c-Jun N-terminal kinases 1/2 (p-JNK1/2) in brain tissues were also significantly strengthened after I/R for 24[Formula: see text]h. All drugs could increase neurocyte survival rate in hippocampal CA1, decrease the apoptotic rate, and inhibit caspase-3 protein expression, in contrast, the effects of four active components combination were better than those of active components alone. In addition, Astragaloside IV and Ginsenoside Rg1 could down-regulate the level of TNF-[Formula: see text], and ICAM-1 mRNA, respectively, Notoginsenoside R1 reduced both TNF-[Formula: see text] and ICAM-1 mRNA, and the combination of the 4 effective components had inhibitory effects on the expressions of TNF-[Formula: see text], IL-1[Formula: see text], and ICAM-1 mRNA. Astragaloside IV, Ginsenoside Rg1, Notoginsenoside R1, and 4 effective components combination were able to restrain the phosphorylation of I[Formula: see text]B[Formula: see text], and relieve the nuclear translocation rate of NF-[Formula: see text]B. Moreover, the effects of the combination are greater than those of active components alone. All drugs could suppress the phosphorylation of JAK1 induced by I/R; meanwhile the expression of p-STAT1 exhibited a decrease in Ginsenoside Rg1 and four active components combination groups. The decreases of p-JAK1 and p-STAT1 in the four active components combination group were more obvious than those in active components alone groups. Astragaloside IV, Ginsenoside Rg1, and Notoginsenoside R1 further augmented GRP78 expression caused by I/R, Notoginsenoside R1 attenuated caspase-12 protein expression, Astragaloside IV and Ginsenoside Rg1 lessened the phosphorylation of JNK1/2, and the four active components combination was capable of up-regulating GRP78 protein while down-regulating the expressions of caspase-12 and p-JNK1/2. Similarly, the effects of the four active components combination were greater than those of effective components alone. These suggested that the combination of the main active components of Astragalus and Panax notoginseng could strengthen protective effects on cerebral ischemia injury via anti-apoptosis and anti-inflammation, and the mechanisms might be associated with restraining the activation of NF-[Formula: see text]B and JAK1/STAT1 signal pathways and regulating endoplasmic reticulum stress (ERS) after cerebral ischemia. </jats:p>
摘要：
目的探讨补阳还五汤对局灶性脑缺血大鼠脑组织TLR4表达的影响。方法72只大鼠随机分为假手术组、模型组和补阳还五汤组,每组按给药后7、14、21 d 3个时间点再各分3组,用免疫组化、Western blot、RT-PCR法检测各组不同时间点TLR4的蛋白和基因表达。结果模型组和补阳还五汤组各时间点TLR4蛋白和mRNA表达均高于假手术组(P<0.05),补阳还五汤组在第7、14天TLR4蛋白表达明显低于模型组,但TLR4mRNA水平在第7天、14天却明显高于模型组,差异均有统计学意义(P<0.05)。结论补阳还五汤通过调节缺血后脑组织TLR4的表达可能是其治疗脑缺血的作用机制之一。
期刊：
Chinese Journal of Tissue Engineering Research,2010年14(23):4285-4289 ISSN：1673-8225
通讯作者：
Yuan, Z.-K.
作者机构：
Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China;[郑景辉; 黄献平; 王丽萍; 袁肇凯; 简维雄] Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China;Li Yong-hua, Studying for doctorate, Attending physician, Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China;Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China [email protected];[李勇华] Department of Traditional Chinese Medicine, Chongqing Three Gorges Medical College, Chongqing 404120, China, Institute of Traditional Chinese Medicine Diagnostic, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China
通讯机构：
[Yuan, Z.-K.] I;Institute of Traditional Chinese Medicine Diagnostic, , Changsha 410007, Hunan Province, China
