作者： Wu, Yi;Zhang, Chenyang;Shao, Haoqing;Luo, Huaihao*;Tan, Zhoujin

期刊： 3 BIOTECH,2021年11(1):1-8 ISSN：2190-572X

通讯作者： Luo, Huaihao;Tan, ZJ

作者机构： [Zhang, Chenyang; Tan, Zhoujin; Luo, Huaihao; Shao, Haoqing; Wu, Yi; Luo, HH] Hunan Univ Chinese Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Luo, HH; Tan, ZJ ] H;Hunan Univ Chinese Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.

关键词： Lily bulb;Intestinal microbiota;Enzyme activity;Functional food

摘要： The aim was to investigate the effect of lily bulbs on the microecological characteristics of intestinal microbiota and enzyme activities in normal mice. Thirty SPF Kunming mice were randomly divided into the control group, Lilium lancifolium (LL) group and Lilium davidii var. unicolor (LDU) group. Mice of the latter two groups were given 0.15g·mL−1 lily bulb solution, respectively, by gavage twice a day, while the control group was given the same volume of sterilized water. After 49days, intestinal contents and mucosa of all mice were collected and the characteristics of intestinal microbiota and enzyme activities were analyzed. Results showed that the number of Lactobacillus spp. and Bifidobacteria spp. in the LL group was significantly higher than that in the control group (t = 2.68 × 107, P = 0.000; t = 5.96 × 107; P = 0.000) and the LDU group (t = 6.12 × 107, P = 0.000; t = 2.71 × 107, P = 0.000), while the number of total bacteria was significantly lower (P = 0.040). Microbial activity in intestinal contents and mucosa of the LDU group (t = 0.43, P = 0.001; t = 0.69, P = 0.000) decreased, and microbial activity in intestinal mucosa of the LL group decreased significantly (t = 0.89, P = 0.000) but increased significantly in intestinal contents of the LL group (t = 0.81, P = 0.000). The activities of amylase (t = 455.73, P = 0.000; t = 206.56, P = 0.000) and protease (t = 52.32, P = 0.000) increased but the activities of lactase (t = 443.51, P = 0.000; t = 15.71, P = 0.000) and sucrase (t = 5.82, P = 0.000; t = 366.82, P = 0.000) decreased significantly in contents from the LL group and LDU group. Except for the sucrase activity, enzyme activities in mucosa of the LL group were completely opposite to those in contents (t = 44.15, P = 0.000; t = 1.25, P = 0.007; t = 14.64, P = 0.011). In conclusion, dietary lily bulbs increased intestinal contents amylase activities and mucosa lactase activity significantly. Lily bulbs, especially Lilium lancifolium, can promote the growth of Lactobacillus spp. and Bifidobacteria spp., and inhibit the growth of total bacteria in the intestines of normal mice. Lilium lancifolium bulbs have the potential to be a functional food. © 2021, King Abdulaziz City for Science and Technology.

语种： 英文

作者： Li, Yuli;Li, Xiaoya;Tan, Zhoujin

期刊： Helicobacter,2021年26(3):e12799- ISSN：1083-4389

通讯作者： Tan, ZJ

作者机构： [Li, Yuli; Li, Xiaoya; Tan, Zhoujin] Hunan Univ Chinese Med, Coll Chinese Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Tan, ZJ ] H;Hunan Univ Chinese Med, Coll Chinese Med, Xueshi Rd 300, Changsha 410208, Hunan, Peoples R China.

关键词： (10) gingerol;(6) gingerol;(8) gingerol;antiinfective agent;baicalein;baicalin;berberine;emodin;glycyrrhizic acid;licochalcone A;licoricidin;matrine;palmatine;quercetin;rhein;scutellarin;unclassified drug;herbaceous agent;Chinese medicine;clinical effectiveness;disease association;drug effect;drug efficacy;drug mechanism;drug response;drug structure;Helicobacter infection;human;nonhuman;priority journal;Review;risk factor;treatment outcome;China;gastritis;Helicobacter pylori;China;Drugs, Chinese Herbal;Gastritis;Helicobacter Infections;Helicobacter pylori;Humans;Medicine, Chinese Traditional

摘要： Background: Helicobacter pylori–associated gastritis (HPAG) is a common digestive system disease that its therapeutic goal is to eradicate Helicobacter pylori. However, due to the widespread use of antibiotics, problems for example, antibiotic resistance, reinfection, and gastrointestinal side effects have emerged. The solution of above problems provides a broad space for traditional Chinese medicine (TCM) to exert its remarkable advantages on the treatment of HPAG. Methods: Extensive database retrieval using platforms not limited to but including Web of Science, SpringerLink, ScienceDirect, Google Scholar, China National Knowledge Infrastructure, Wanfang, and VIP database was performed using keywords such as “Helicobacter pylori-associated gastritis” or “HPAG” or “Helicobacter pylori” or “H. pylori” or “gastritis” and “traditional Chinese medicine” or “TCM” or “herbs” or “Chinese herbal medicine”. In addition, related books, PhD, and master's dissertations were also researched to provide a comprehensive review. Results: This review mainly introduces the clinical efficacy of TCM formulas for HPAG, as well as active ingredient and pharmacological mechanisms of herbs. What's more, this review puts forward potential prospects for future research. Conclusion: These research works have shown the therapeutic benefits of TCM in the treatment of HPAG. The development of TCM with more specific functions and practical data will not only become a significant trend in the world market but also have an irreplaceable role in the future treatment of HPAG. More continued researches should be undertaken in the future. © 2021 John Wiley & Sons Ltd

语种： 英文

作者： 吴仪;唐圆;刘娅薇;惠华英;谭周进#&#&#WU Yi;...

期刊： 中国微生态学杂志,2020年32(7):745-749 ISSN：1005-376X

作者机构： [吴仪; 唐圆; 惠华英; 刘娅薇] 湖南中医药大学;[TAN Zhoujin] 中医方证研究转化医学湖南省重点实验室

关键词： 痛泻要方;“肝气乘脾”泄泻;肠道酶活性;肠道微生态;中医证型

摘要： 目的探讨痛泻要方对"肝气乘脾"泄泻小鼠肠道酶活性的影响。方法采用"番泻叶-离心管束缚夹尾法"进行"肝气乘脾"泄泻造模,造模成功后以痛泻要方治疗,造模和治疗后分别分析小鼠肠道酶活性。结果造模后,模型组小鼠肠道内容物的淀粉酶活性显著降低(t=4.007,P=0.015),纤维素酶、蛋白酶、蔗糖酶活性下降不显著。模型组小鼠肠黏膜蛋白酶、淀粉酶、蔗糖酶活性显著下降(t_蛋=5.652,P=0.005;Z_淀=-1.964,P=0.050;t_蔗=4.737,P=0.009)。痛泻要方治疗后,中药干预组小鼠肠道内容物蛋白酶、纤维素酶、乳糖酶和蔗糖酶活性变化不显著;自然恢复组的淀粉酶活性显著高于正常组(t=-7.497,P=0.002)。中药干预组小鼠肠道前段黏膜蛋白酶、乳糖酶、淀粉酶、蔗糖酶活性恢复不显著;中药干预组小鼠肠道黏膜中段乳糖酶、蔗糖酶活性显著低于自然恢复组(t_乳=4.074,P=0.013;t_蔗=8.072,P<0.001),而蛋白酶、淀粉酶及纤维素酶活性均高于自然恢复组;中药干预组小鼠肠道后段黏膜乳糖酶、蔗糖酶及淀粉酶活性显著高于正常组(t_乳=-7.962,P<0.001;t_蔗=-15.921,P<0.001;Z_淀=6.489,P=0.034),蛋白酶与纤维素酶活性均有所升高。结论"肝气乘脾"泄泻肠道内容物及黏膜淀粉酶活性显著降低,痛泻要方对"肝气乘脾"泄泻小鼠肠黏膜乳糖酶、蔗糖酶及淀粉酶活性作用显著。

语种： 中文

作者： 李玉丽;肖嫩群;谭周进#&#&#Yu-Li Li;Nen-Qun Xiao;Zhou-Jin Tan

期刊： 世界华人消化杂志,2020年28(23):1192-1199 ISSN：1009-3079

作者机构： [李玉丽; 肖嫩群; 谭周进#&#&#Yu-Li Li] 湖南中医药大学药学院,湖南中医药大学医学院 湖南省长沙市410208

关键词： 代谢性疾病;肠道菌群;肥胖;糖尿病;非酒精性脂肪肝

摘要： 代谢性疾病是指葡萄糖、蛋白质及脂质在体内代谢异常引起的一类疾病.越来越多的证据支持肠道菌群失调与代谢性疾病具有重要的相关性.具体而言,肠道菌群失调可能破坏肠道屏障功能,诱发内毒素血症、胆汁酸代谢紊乱和全身慢性低度炎症,促进代谢性疾病进展及并发症发生.随着代谢性疾病病情进展,肠道内环境改变以及药物、饮食等影响又反过来加重肠道菌群失调,最终引起预后不良.这凸显了通过调节肠道菌群结构来开发防治代谢性疾病治疗策略的可能性.益生菌、益生元和合生元疗法、粪菌移植、中医药等可通过维持肠道菌群平衡、对抗有害菌群产物,从而治疗代谢性疾病.本文从代谢性疾病肠道菌群变化、肠道菌群影响代谢性疾病的可能机制、肠道菌群在代谢性疾病治疗中的应用等方面进行综述,以期为以肠道菌群为靶点的代谢性疾病的诊断防治研究提供借鉴.

语种： 中文

作者： 何云山;吴仪;谭周进#&#&#HE Yun-shan;WU Yi;TAN Zhou-jin

期刊： 现代农业科技,2020年(9):208,214 ISSN：1007-5739

作者机构： [何云山; 吴仪; 谭周进#&#&#HE Yun-shan] 湖南中医药大学

关键词： 乳糖酶;乳糖不耐受;仔猪腹泻

摘要： 乳糖是仔猪的主要能量来源,乳糖酶对乳糖的消化吸收起着至关重要的作用。乳糖酶缺乏导致乳糖不耐受,产生肠胃胀气、腹痛甚至腹泻等症状,严重影响着仔猪的成长。本文围绕猪乳糖酶的来源、乳糖酶缺乏与腹泻的关系以及乳糖酶缺乏的治疗展开综述,以期为仔猪的饲养提供参考。

语种： 中文

作者： 唐圆;吴仪;惠华英;谭周进#&#&#TANG Yuan;WU Yi;...

期刊： 中国微生态学杂志,2020年32(01):17-20 ISSN：1005-376X

作者机构： [吴仪; 唐圆; 惠华英; 谭周进#&#&#TANG Yuan] 湖南中医药大学

关键词： 痛泻要方;肝气乘脾泄泻;肠道微生物活度;荧光素二乙酸

摘要： 目的探讨痛泻要方对肝气乘脾泄泻小鼠肠道微生物活度的影响,为痛泻要方通过调节肠道微生物治疗肝气乘脾泄泻提供实验基础和理论依据。方法将25只SPF级雄性昆明小鼠随机分为正常组10只和模型组15只,造模成功后,随机选取正常组和模型组动物各5只,在无菌环境下提取肠道内容物和肠黏膜,剩余模型动物随机分为自然恢复组和治疗组各5只,治疗成功后处死各组动物提取肠道内容物和肠黏膜。用荧光素二乙酸法测定各样品中微生物活度。结果肝气乘脾泄泻造模小鼠肠道内容物微生物活度高于正常组,与正常组相比差异有统计学意义(P=0.01),而模型组小鼠肠黏膜微生物活度降低,与正常组相比差异有统计学意义(P=0.01)。痛泻要方治疗组和自然恢复组小鼠肠道内容物微生物活度均降低,与正常组相比差异无统计学意义(P>0.05),治疗组和自然恢复组小鼠的肠黏膜前段和后段均与正常组差异有统计学意义(P=0.001),肠黏膜中段自然恢复组与正常组差异有统计学意义(P=0.043),而治疗组与正常组没有差异。结论肝气乘脾泄泻造模使肠道内容物微生物活度增加,而使肠黏膜微生物活度降低。痛泻要方能够使肝气乘脾泄泻小鼠肠道内容物中微生物活度降低,恢复至正常水平,增加肠黏膜中段微生物活度,对肠黏膜后段微生物活度增加作用更显著。

语种： 中文

作者： Zhang Chenyang;Shao Haoqing;Li Dandan;Xiao Nenqun;Tan Zhoujin

期刊： BMC Microbiology,2020年20(1):1-17 ISSN：1471-2180

通讯作者： Tan, ZJ;Xiao, Nenqun

作者机构： [Zhang Chenyang; Shao Haoqing] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhang Chenyang; Tan, Zhoujin; Shao Haoqing; Tan Zhoujin] Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;[Xiao Nenqun; Tan, Zhoujin; Xiao, NQ; Tan Zhoujin; Li Dandan] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.

通讯机构： [Xiao, NQ; Tan, ZJ ] H;Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.

关键词： Diarrhea;Intestinal microbiota;Cytochrome P450;Tryptophan metabolism;Folium sennae

摘要： Although reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality. For the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased. The Beta diversity clusters of mice interfered by Folium sennae extracts were distinctly separated from control group. Combining PPI network analysis, cytochrome P450 enzymes metabolism was the main signaling pathway of diarrhea caused by Folium sennae. Moreover, 10 bacterial flora communities had statistical significant difference with Folium sennae intervention: the abundance of Paraprevotella, Streptococcus, Epulopiscium, Sutterella and Mycoplasma increased significantly; and the abundance of Adlercreutzia, Lactobacillus, Dehalobacterium, Dorea and Oscillospira reduced significantly. Seven of the 10 intestinal microbiota communities were related to the synthesis of tryptophan derivatives, which affected the transformation of aminotryptophan into L-tryptophan, leading to abnormal tryptophan metabolism in the host. Folium sennae targeted cytochrome P450 3A4 to alter intestinal bacterial characteristic and intervene the tryptophan metabolism of intestinal microbiota, such as Streptococcus, Sutterella and Dorea, which could be the intestinal microecological mechanism of diarrhea caused by Folium sennae extracts.

语种： 英文

作者： 何云山;刘娅薇;谭周进;惠华英#&#&#HE Yunshan;LIU Yawei;...

期刊： 应用化学,2020年37(2):242-244 ISSN：1000-0518

通讯作者： Huaying, H.

作者机构： 湖南中医药大学药学院 长沙410208;湖南中医药大学医学院 长沙410208;[何云山; 惠华英#&#&#HE Yunshan; 谭周进; 刘娅薇] 湖南中医药大学

通讯机构： [Huaying, H.] C;College of Pharmacy, China

关键词： 邻硝基苯-β-D-吡喃半乳糖苷法;双糖酶法;乳糖酶活力测定;方法学比较

摘要： 邻硝基苯-β-D-吡喃半乳糖苷( ONPG)法和双糖酶法是常用的乳糖酶活力测定方法,本文对这两种方法的线性范围、检出限、精密度、准确度和稳定性进行了比较。结果显示,ONPG法在线性范围、检出限、精密度以及稳定性方面均优于双糖酶法,但此种方法的重复性较差,需要进一步的优化。对比这两种方法,可为乳糖酶活力测定方法的合理选取提供参考。

语种： 中文

作者： 何云山;谭周进;惠华英#&#&#HE Yun-shan;TAN Zhou-jin;HUI Hua-ying

期刊： 现代中药研究与实践,2020年34(01):77-81 ISSN：1673-6427

作者机构： 湖南中医药大学药学院,湖南长沙410208;湖南中医药大学医学院,湖南长沙410208;[何云山; 惠华英#&#&#HE Yun-shan; 谭周进] 湖南中医药大学

关键词： 保和丸;药理作用;临床应用;肠道菌群

摘要： 保和丸是临床常用的消食导滞药物,出自朱丹溪所著的《丹溪心法》。保和丸的主要成分为有机酸、橙皮苷以及连翘苷等,具有增加胃肠运动、提高胃蛋白酶活性、增加消化液分泌、抗动脉粥样硬化、抗炎、降血脂等药理作用。临床主要用于治疗与食积相关的疾病,对脂肪肝、高脂血症也有较好的疗效,对肠道菌群具有一定的作用。文章综述了保和丸的化学组成、药理作用、临床应用及与肠道菌群的作用,为保和丸的临床应用与实验研究提供一定的参考。

语种： 中文

作者： 吴仪;谭周进;李丹丹;惠华英#&#&#WU Yi;TAN Zhoujin;...

期刊： 中国微生态学杂志,2020年32(11):1337-1340 ISSN：1005-376X

作者机构： [吴仪; 李丹丹; 惠华英#&#&#WU Yi; 谭周进] 湖南中医药大学

关键词： 天人相应;微生态;生态学思想;微生态制剂

摘要： 中医天人相应观是中医学的指导思想,贯穿中医学体系始终,体现了中医学的生态学理念。微生态学作为一门新兴的学科,属于生态医学的范畴,尤其是近年对微生态学的深入研究,发现其研究结果与中医学观点存在共同之处,并已有学者论证二者在理论和实践上都具有一定的统一性,为中医药现代化提供了一定的理论依据。本文回顾性分析了中医天人相应学说和微生态学内容,发现二者在理论起源、发病机制、防治和应用方面具有一定的统一性,并且创新性地从微生态学角度解读中医天人相应学说,从而论证中医学说的正确性和现实性,也为中西医的进一步融合提供新的思路。

语种： 中文

作者： 唐圆;谭周进#&#&#Yuan Tang;Zhou-Jin Tan

期刊： 世界华人消化杂志,2020年28(15):719-724 ISSN：1009-3079

作者机构： [唐圆; 谭周进#&#&#Yuan Tang] 湖南中医药大学

关键词： 中医药治疗;肠道微生态;肠道菌群;针灸

摘要： 中医药(traditional Chinese medicine,TCM)治疗(中药治疗、针灸治疗、推拿治疗等)在临床上应用广泛、疗效显著,同时越来越多的研究表明众多疾病的预防、发生和治疗都与肠道微生态有关,TCM与肠道微生态关系的研究也越来越多.TCM治疗的基础理论与微生态学的很多理论和观点一致,从两者一致的基础理论出发,对TCM治疗与肠道微生态的关系进行综述,对从肠道微生态出发探讨TCM治疗临床疾病的机理具有重要意义.本文论述TCM治疗与肠道微生态的关系,以及TCM治疗对肠道微生态的影响,以此为中医治疗的传统手段治疗肠道微生态相关的疾病提供新的理论与依据.旨在促进TCM的现代化研究,促进TCM的临床应用和全球化.

语种： 中文

作者： 李玉丽;吴仪;袁振仪;谭周进#&#&#Yu-Li Li;Yi Wu;...

期刊： 世界华人消化杂志,2020年28(21):1090-1095 ISSN：1009-3079

作者机构： 湖南中医药大学微生物教研室 湖南省长沙市410208;[李玉丽; 吴仪; 谭周进#&#&#Yu-Li Li; 袁振仪] 湖南中医药大学

关键词： 泄泻;同病异治;异病同治;辨证论治

摘要： \"同病异治\"是中医辨证论治思想的具体体现,古今医家广泛临证应用.泄泻作为一种中医常见病,病因复杂多样,在病机上又有表里寒热虚实的差异,虽同为泄泻之病,却存在不同的证型,在临床上形成了\"同病异治\".本文将从分析泄泻的\"同病异治\"之\"病\"、\"治\"的内涵外延切入,从辨病因、辨病机、辨发病季节、辨病者体质四个方面来论述\"同病异治\"在泄泻中的具体应用,以期了解\"同病异治\"的实质,指导泄泻的临证施方.

语种： 中文

作者： 谢果珍;唐圆;姜珊;罗帅;谭周进#&#&#XIE Guo-zhen;...

期刊： 时珍国医国药,2020年31(06):1351-1354 ISSN：1008-0805

作者机构： [唐圆; 谭周进#&#&#XIE Guo-zhen; 谢果珍; 罗帅; 姜珊] 湖南中医药大学

关键词： 七味白术散;总苷;均匀设计法;最优工艺;体外抑菌

摘要： 目的比较优化七味白术散总苷的提取工艺及初步探究其体外抑菌活性。方法采用均匀设计法,以乙醇浓度、乙醇用量、提取时间、超声功率为考察因素,以总苷量及人参皂苷量加权得到的综合评分为评价指标,通过二次逐步回归分析筛选水提醇沉法及超声辅助提取法从七味白术散汤剂中提取总苷的最优提取工艺,并对七味白术散总苷在体外抑制大肠杆菌及酵母菌的活性进行初步研究。结果研究得到水提醇沉法的优化提取工艺为:用14倍量的68%乙醇提取15 h,该工艺的浸膏得率为29.28%,并得到总苷0.28 g,人参皂苷0.0871 g。超声辅助提取法的优化提取工艺为: 4倍量的56%乙醇在60%超声功率下提取40 min,浸膏得率为31.99 %,得到总苷0.30 g,人参皂苷0.0917 g。结论综合考虑成本及提取效率,选择超声辅助提取法的最优工艺作为七味白术散总苷的制备方法。七味白术散总苷对大肠杆菌( Escherichia coli)及酵母菌( Saccharomycetes)均表现出抑制作用。

语种： 中文

作者： 杨晓燕;何云山;谭周进;肖丹;曾钰婷;...

期刊： 食品与发酵工业,2020年46(20):101-106 ISSN：0253-990X

作者机构： [何云山; 曾钰婷; 肖丹; 杨晓燕; 谭周进; 曾晨#&#&#YANG Xiaoyan] 湖南中医药大学

关键词： 植物油;肠道微生物;酶活性;血常规;肠道微生态

摘要： 为探讨不同剂量植物油对小鼠肠道微生物、酶活性及血常规的影响,将30只无特定病原体（specific pathogen free,SPF）级昆明小鼠随机分为5组,分别灌胃6.67 m L/（kg·d）（0.1 m L/次）、13.33 m L/（kg·d）（0.2m L/次）、20.00 m L/（kg·d）（0.3 m L/次）及26.67 m L/（kg·d）（0.4 m L/次）植物油,以0.2 m L蒸馏水/次为正常组,2次/d,共30 d,分析血常规、肠道微生物及酶活性,结果表明,植物油能显著降低小鼠肠道细菌总数和大肠杆菌数（P <0.05）。6.67 m L/（kg·d）组和26.67 m L/（kg·d）组小鼠肠道乳酸菌数显著低于正常组（P <0.05）,6.67m L/（kg·d）组和20.00 m L/（kg·d）组双歧杆菌数较正常组显著降低（P <0.05）,而13.33 m L/（kg·d）组双歧杆菌数较正常组显著增加（P <0.05）。6.67 m L/（kg·d）组小鼠的淀粉酶、蛋白酶、木聚糖酶以及纤维素酶活性显著高于正常组（P <0.05）,当食用油剂量增加时,4种酶的活性出现不同程度的下降。不同剂量植物油对正常小鼠的红细胞、血小板、血红蛋白以及白细胞等相关指标基本无影响,仅26.67 m L/（kg·d）组的淋巴细胞百分比、中性粒细胞百分比与正常组差异显著（P <0.05）。该文证明适当摄入植物油有助于增加益生菌数,提高肠道消化酶活性。

语种： 中文

作者： 刘娅薇;吴仪;惠华英;谭周进#&#&#LIU Yawei;WU Yi;...

期刊： 应用与环境生物学报,2020年26(4):1023-1027 ISSN：1006-687X

通讯作者： HUI, H.;TAN, Z.

作者机构： 湖南中医药大学 长沙410208;中医方证研究转化医学湖南省重点实验室 长沙410208;[吴仪; 惠华英; TAN Zhoujin; 刘娅薇] 湖南中医药大学

通讯机构： [HUI, H.; TAN, Z.] H;Hunan University of Traditional Chinese MedicineChina

关键词： 痛泻要方;肝气乘脾泄泻;D-木糖;胃动力;血生化

摘要： 为建立小鼠肝气乘脾泄泻的造模方法,并通过痛泻要方对其疗效进行验证.采用番泻叶加束缚夹尾法制备肝气乘脾泄泻小鼠模型,运用痛泻要方进行治疗,以主观和客观指标进行综合评价.结果显示,番泻叶-束缚夹尾使模型小鼠出现情绪亢奋,腹泻程度严重,进食量减少,体重增长幅度下降,毛发变暗、枯黄,同时甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、5-羟色胺(5-HT)含量升高(P > 0.05),D-木糖含量下降(P < 0.05),符合脾虚证诊断标准,同时出现肠推进率增高(P < 0.05)、胃残留率降低(P > 0.05)等小肠功能亢进的表现.经痛泻要方干预后小鼠一般行为接近正常组,腹泻症状明显消失,总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)(P < 0.05)、TG、LDL-C相对正常组含量下降(P > 0.05),模型组相对自然恢复组的5-HT含量减少(P < 0.01),而D-木糖含量出现一定程度的升高(P > 0.05),痛泻要方能调节模型小鼠的胃肠运动,降低其肠推进率,升高胃残留率(P > 0.05).本研究表明通过番泻叶-束缚夹尾法上午灌胃番泻叶造成小鼠脾虚泄泻,下午离心管束缚夹尾模拟小鼠肝旺状态,连续造模7 d,可以建立小鼠肝气乘脾泄泻模型,痛泻要方干预3 d可以治愈,5-HT增高、D-木糖降低、胃动力减缓等能作为小鼠肝气乘脾泄泻的评价指标;结果可为小鼠肝气乘脾泄泻模型的建立提供可靠的实验依据.

语种： 中文

作者： 何云山;谭周进;惠华英

期刊： 湖北中医药大学学报,2020年22(03):121-125 ISSN：1008-987X

作者机构： 湖南中医药大学药学院,湖南长沙410208;湖南中医药大学医学院,湖南长沙410208

关键词： 泄泻;食滞胃肠;动物模型;评价指标

摘要： 人类疾病动物模型在认识生命科学客观规律中扮演着重要的角色,中医动物模型建立是中医药研究现代化进程的重要推动力。食滞胃肠泄泻是中医泄泻当中常见的一种类型,多因饮食不节、暴饮暴食而致宿食内停、阻滞肠胃。在中医基础理论指导下,建立符合中医病因病机的食滞胃肠泄泻动物模型是开展该病基础研究工作的重要载体。分析食滞胃肠相关动物模型研究现状、食滞胃肠泄泻的中医认识,结合食滞、脾虚与泄泻三者之间的关系,为食滞胃肠泄泻动物模型的制备提供思路。

语种： 中文

作者： Shao, Haoqing;Zhang, Chenyang;Wang, Chunhui;Tan, Zhoujin

期刊： 3 BIOTECH,2020年10(9):1-11 ISSN：2190-572X

通讯作者： Tan, ZJ;Wang, CH

作者机构： [Zhang, Chenyang; Tan, Zhoujin; Shao, Haoqing] Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;[Wang, Chunhui; Wang, CH] Hunan Edible Fungus Res Inst, Changsha, Hunan, Peoples R China.

通讯机构： [Tan, ZJ ; Wang, CH ] H;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.;Hunan Edible Fungus Res Inst, Changsha, Hunan, Peoples R China.

关键词： Debaryomyces hansenii;Qiweibaizhu powder;Intestinal mucosa;SCFA-producing bacteria;Antibiotic-associated diarrhea

摘要： The aim was to investigate the combined effect of Debaryomyces hansenii and Qiweibaizhu powder (QWBZP) on the bacterial diversity of the intestinal mucosa of antibiotic-associated diarrhea (AAD) mice, for the potential treatment of diarrhea, especially which is induced by administration of antibiotics. Eighteen (18) mice were randomly assigned to three equal groups of six mice, namely Normal (mn group), Placebo control (mm group) and D. hansenii and QWBZP (DQ) treatment (mdq group). Mice were gavaged with a solution (23.33mL·kg−1·day−1) consisting of gentamicin and cefradine to establish AAD. The DQ treatment group was gavaged with DQ for 4days, and sterile water was used as a placebo control. The metagenome DNA of the intestinal mucosal microbiota was extracted, and the 16S rRNA gene was sequenced. Analysis showed that there were 288 OTUs for the normal group, 443 for the placebo control group, and 229 for the DQ treatment group. Phylogenetically, the gut microbiota of the DQ treatment group and the normal group were closer to each other than to the placebo control group. Both the DQ and placebo-treated groups included Stenotrophomonas, Robinsoniella, Bacteroidales S24-7 group norank, Citrobacter, and Glutamicibacter, but their abundances were significantly higher in the DQ treatment group than in the placebo control group. This suggested that the combined use of D. hansenii and QWBZP overcame the influence of dysbacteriosis and could lead to the recovery of intestinal mucosal microbiota homeostasis. This positive effect is likely related to short-chain fatty acid (SCFA)-producing bacteria, such as members of Micrococcaceae, Lachnospiraceae, and Bacteroidales S24-7 group, which could play beneficial roles in protecting the mucosal barrier and stimulating the immune response in mice. © 2020, King Abdulaziz City for Science and Technology.

语种： 英文

作者： Shao Haoqing;Zhang Chenyang;Xiao Nenqun;Tan Zhoujin

期刊： BMC Microbiology,2020年20(1):1-9 ISSN：1471-2180

通讯作者： Tan, ZJ

作者机构： [Zhang Chenyang; Shao Haoqing] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhang Chenyang; Tan, Zhoujin; Shao Haoqing; Tan Zhoujin] Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;[Xiao Nenqun] Hunan Univ Chinese Med, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Tan, Zhoujin; Tan Zhoujin] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.

通讯机构： [Tan, ZJ ] H;Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.

关键词： Gut microbiota;Antibiotic-associated diarrhea;Gentamicin;Cefradine;Enterococcus;Clostridium;16S rRNA gene sequencing

摘要： Antibiotic-associated diarrhea (AAD), defined as diarrhea that occurs in association with the administration of antibiotics and without another clear etiology, is one of the most common adverse drug events of antibiotics therapy. We established a diarrhea model induced by gentamycin and cefradine to investigate the microbiota characteristics in the intestinal lumen of mice with AAD and provide insights into noteworthy bacteria related to gentamicin and cefradine-associated diarrhea. The number of OTUs in the model group and the normal group was 983 and 2107, respectively, and 872 identical OTUs were shared between two groups. Species richness and species diversity of intestinal microbe were altered by antibiotics administration. PCoA showed a clear separation between AAD and health control. The dominant phyla of AAD mice were Firmicutes (52.63%) and Proteobacteria (46.37%). Among the genus with top 20 abundance, the relative abundance of 7 genera, Ruminococcus, Blautia, Enterococcus, Eubacterium, Clostridium, Coprococcus, and Aerococcus, were enriched in the model group. Based upon the LEfSe analysis, Enterococcus, Eubacterium, Ruminococcus, and Blautia were identified as potential biomarkers for AAD. The bacterial diversity of the intestinal lumen was diminished after gentamicin and cefradine administration. The alterations in the abundance and composition of gut microbiota further led to the dysfunction of gut microbiota. More specifically, gentamicin and cefradine significantly increased the abundance of the opportunistic pathogens, of which Enterococcus and Clostridium were the most prominent and most worthy of attention.

语种： 英文

作者： 何云山;谭周进#&#&#HE Yun-shan;TAN Zhou-jin

期刊： 现代农业科技,2020年(4):214-215 ISSN：1007-5739

作者机构： [何云山; 谭周进#&#&#HE Yun-shan] 湖南中医药大学

关键词： 铁皮石斛;超微中药;粗多糖

摘要： 通过苯酚-硫酸法测定铁皮石斛超微粉与铁皮石斛常规粉水溶性粗多糖的含量,探讨超微技术对铁皮石斛粗多糖浸出的影响.结果 表明,铁皮石斛超微粉中水溶性粗多糖含量为484.6 mg/g,常规粉水溶性粗多糖含量为370.2 mg/g,铁皮石斛超微粉水溶性粗多糖含量比常规粉水溶性粗多糖含量高30.9％,二者的差异具有统计学意义(P＜0.05).超微技术有助于铁皮石斛粗多糖的浸出.

语种： 中文

项目作者： 谭周进

项目作者单位： 湖南中医药大学

项目批准号： 81874460

资助经费： 57万

立项时间： 2019

项目级别： 国家级

项目来源： 国家自然科学基金