摘要：
Although reports have provided evidence that diarrhea caused by Folium sennae can result in intestinal microbiota diversity disorder, the intestinal bacterial characteristic and specific mechanism are still unknown. The objective of our study was to investigate the mechanism of diarrhea caused by Folium sennae, which was associated with intestinal bacterial characteristic reshaping and metabolic abnormality. For the intervention of Folium sennae extracts, Chao1 index and Shannon index were statistical decreased. The Beta diversity clusters of mice interfered by Folium sennae extracts were distinctly separated from control group. Combining PPI network analysis, cytochrome P450 enzymes metabolism was the main signaling pathway of diarrhea caused by Folium sennae. Moreover, 10 bacterial flora communities had statistical significant difference with Folium sennae intervention: the abundance of Paraprevotella, Streptococcus, Epulopiscium, Sutterella and Mycoplasma increased significantly; and the abundance of Adlercreutzia, Lactobacillus, Dehalobacterium, Dorea and Oscillospira reduced significantly. Seven of the 10 intestinal microbiota communities were related to the synthesis of tryptophan derivatives, which affected the transformation of aminotryptophan into L-tryptophan, leading to abnormal tryptophan metabolism in the host. Folium sennae targeted cytochrome P450 3A4 to alter intestinal bacterial characteristic and intervene the tryptophan metabolism of intestinal microbiota, such as Streptococcus, Sutterella and Dorea, which could be the intestinal microecological mechanism of diarrhea caused by Folium sennae extracts.
作者机构：
[Yunshan H.; Huaying H.] College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208, China;[Yawei L.; Zhoujin T.] School of Medicine, Hunan University of Chinese Medicine, Changsha, 410208, China
摘要：
中医药(traditional Chinese medicine,TCM)治疗(中药治疗、针灸治疗、推拿治疗等)在临床上应用广泛、疗效显著,同时越来越多的研究表明众多疾病的预防、发生和治疗都与肠道微生态有关,TCM与肠道微生态关系的研究也越来越多.TCM治疗的基础理论与微生态学的很多理论和观点一致,从两者一致的基础理论出发,对TCM治疗与肠道微生态的关系进行综述,对从肠道微生态出发探讨TCM治疗临床疾病的机理具有重要意义.本文论述TCM治疗与肠道微生态的关系,以及TCM治疗对肠道微生态的影响,以此为中医治疗的传统手段治疗肠道微生态相关的疾病提供新的理论与依据.旨在促进TCM的现代化研究,促进TCM的临床应用和全球化.
摘要：
为探讨不同剂量植物油对小鼠肠道微生物、酶活性及血常规的影响,将30只无特定病原体（specific pathogen free,SPF）级昆明小鼠随机分为5组,分别灌胃6.67 m L/（kg·d）（0.1 m L/次）、13.33 m L/（kg·d）（0.2m L/次）、20.00 m L/（kg·d）（0.3 m L/次）及26.67 m L/（kg·d）（0.4 m L/次）植物油,以0.2 m L蒸馏水/次为正常组,2次/d,共30 d,分析血常规、肠道微生物及酶活性,结果表明,植物油能显著降低小鼠肠道细菌总数和大肠杆菌数（P <0.05）。6.67 m L/（kg·d）组和26.67 m L/（kg·d）组小鼠肠道乳酸菌数显著低于正常组（P <0.05）,6.67m L/（kg·d）组和20.00 m L/（kg·d）组双歧杆菌数较正常组显著降低（P <0.05）,而13.33 m L/（kg·d）组双歧杆菌数较正常组显著增加（P <0.05）。6.67 m L/（kg·d）组小鼠的淀粉酶、蛋白酶、木聚糖酶以及纤维素酶活性显著高于正常组（P <0.05）,当食用油剂量增加时,4种酶的活性出现不同程度的下降。不同剂量植物油对正常小鼠的红细胞、血小板、血红蛋白以及白细胞等相关指标基本无影响,仅26.67 m L/（kg·d）组的淋巴细胞百分比、中性粒细胞百分比与正常组差异显著（P <0.05）。该文证明适当摄入植物油有助于增加益生菌数,提高肠道消化酶活性。
作者机构：
湖南中医药大学 长沙410208;中医方证研究转化医学湖南省重点实验室 长沙410208;[吴仪; 刘娅薇; 谭周进; 惠华英] Hunan University of Traditional Chinese Medicine, Changsha, 410208, China, Hunan Key Laboratory of TCM Prescription and Syndromes Translational Medicine, Changsha, 410208, China
通讯机构：
[HUI, H.; TAN, Z.] H;Hunan University of Traditional Chinese MedicineChina
作者机构：
[Zhang, Chenyang; Shao, Haoqing] Hunan Univ Chinese Med, Sch Tradit Chinese Med, Changsha, Hunan, Peoples R China.;[Zhang, Chenyang; Tan, Zhoujin; Shao, Haoqing] Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;[Xiao, Nenqun] Hunan Univ Chinese Med, Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Tan, Zhoujin] Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Tan, ZJ ] H;Hunan Key Lab TCM Prescript & Syndromes Translat, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Med, Changsha, Hunan, Peoples R China.
关键词：
Gut microbiota;Antibiotic-associated diarrhea;Gentamicin;Cefradine;Enterococcus;Clostridium;16S rRNA gene sequencing
摘要：
Antibiotic-associated diarrhea (AAD), defined as diarrhea that occurs in association with the administration of antibiotics and without another clear etiology, is one of the most common adverse drug events of antibiotics therapy. We established a diarrhea model induced by gentamycin and cefradine to investigate the microbiota characteristics in the intestinal lumen of mice with AAD and provide insights into noteworthy bacteria related to gentamicin and cefradine-associated diarrhea. The number of OTUs in the model group and the normal group was 983 and 2107, respectively, and 872 identical OTUs were shared between two groups. Species richness and species diversity of intestinal microbe were altered by antibiotics administration. PCoA showed a clear separation between AAD and health control. The dominant phyla of AAD mice were Firmicutes (52.63%) and Proteobacteria (46.37%). Among the genus with top 20 abundance, the relative abundance of 7 genera, Ruminococcus, Blautia, Enterococcus, Eubacterium, Clostridium, Coprococcus, and Aerococcus, were enriched in the model group. Based upon the LEfSe analysis, Enterococcus, Eubacterium, Ruminococcus, and Blautia were identified as potential biomarkers for AAD. The bacterial diversity of the intestinal lumen was diminished after gentamicin and cefradine administration. The alterations in the abundance and composition of gut microbiota further led to the dysfunction of gut microbiota. More specifically, gentamicin and cefradine significantly increased the abundance of the opportunistic pathogens, of which Enterococcus and Clostridium were the most prominent and most worthy of attention.
