作者机构：
[曹俊岭] Institute of Chinese Medicine, 302 Hospital of PLA, Beijing 100039, China. cj10506@sina.com
关键词：
人参皂苷;含量测定
摘要：
目的:为8种人参皂苷的含量测定建立一套方法.方法:色谱柱:ODS柱(Kromasil 250mm×4.6mm,5μm),DAD检测器;流动相:A为乙腈,B为水,梯度洗脱0～35 min 19%A,35～55 min 19%～29%A,55～75 min29%A,75～100 min 29%～40%A.流速:1 ml/min,温度:室温,检测波长:203 nm.结果:用本方法对各种皂苷的测定结果:稳定性试验RSD为0.55%～2.26%,精密度试验RSD为0.85%～1.93%,重复性试验RSD为0.97%～2.72%.结论:本法稳定性好,精确度高,重复性好,可作为测定这8种皂苷的一个较好的方法.
作者机构：
College of Pharmacy, Hunan College of Traditional Chinese Medicine, Changsha 410007, China;First Hospital Attached to Hunan College of Traditional Chinese Medicine, Changsha 410007, China;[郭建生] College of Pharmacy, Hunan College of Traditional Chinese medicine, Changsha 410007, China
通讯机构：
College of Pharmacy, Hunan College of Traditional Chinese Medicine, China
通讯机构：
[Yan, Jie] H;Hunan Coll Tradit Chinese Med, Inst Acupuncture & Moxibust, Physiol Lab, Changsha 410007, Peoples R China.
关键词：
NTS;Firing Neuron;Gastric Distention;Meridian and Viscera;Acupuncture
摘要：
The nucleus of the solitary tract (NTS) is a primary center, not only for receiving visceral afferents, but also for somatic afferents. Previous experiments demonstrated that NTS might be a primary afferent center for connecting the Stomach Meridian (ST Medirian) and the Stomach. In this study, an extracellular recording method was used to analyze the firing neurons. The variety of NTS' neurons' responses to gastric distention (GD) and different body surface points were compared. In 52 rats, 104 firing neurons of NTS were recorded, in which there were 47 GD related neurons. For the 47 GD related neurons, there were 33 neurons responsive to acupuncture at Sibai (ST 2, belonging to ST Meridian). Therefore, in the 47 GD related neurons, the response neuron occurring rate (RNOR) for acupuncture at ST 2 was 70.21%. Meanwhile in the 47 GD related neurons, when acupuncture was performed at Quanliao (SI 18, belonging to Small Intestine Meridian) and non-acupoint respectively, there were 18 and 17 firing neurons, and the RNOR were 38.30% and 36.17% respectively. The experiment demonstrated that there were some varieties in the response of NTS neurons to GD stimuli and acupuncture at different body surface points, which suggested that the ST Meridian was more intimately linked to the Stomach and that the different acupoints do have some specificity.
作者机构：
[杨正望] Department of Gynaecology and Obstetrics, First Hospital, Hunan College of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China. yxw321@sohu.com
作者机构：
[申玉华] Department of Obstetrics and Gynecology, Third Hospital, Hunan College of Traditional Chinese Medicine, Zhuzhou, Hunan Province 412000, China. syhdoctor@163.com
作者机构：
[陈懿; 葛金文; 邓冰湘] Department of Physiology Hunan University of Traditional Chinese Medicine,Changsha,410007,China;[陈懿; 葛金文; 邓冰湘] Lab of Vascular Biology,Hunan University of Traditional Chinese Medicine,Changsha,410007,China;[陈懿; 葛金文; 邓冰湘] Department of Physiology,Hunan University of Traditional Chinese Medicine,Changsha,410007,China
通讯机构：
[Ge Jin-wen] D;Department of Science and Technology, Hunan University of Traditional Chinese Medicine, Changsha
摘要：
OBJECTIVE: A new rat model of cerebral infarction was developed to elucidate the contribution of vascular endothelial cell during focal cerebral infarction formation. METHODS: Forty-eight Sprague-Dawley (SD) rats were randomly divided into the model group, sham operation group, and control group for indexes observation of triphenyltetrazolium chloride (TTC) dyeing, neurological deficit, plasma tissue-type plasminogen activator (tPA) activity, plasminogen activator inhibitor (PAI) activity, thromboxane B(2) (TXB(2)) content, and 6-keto-prostaglandin (6-keto-PGF(1alpha)) content. RESULTS: (1) The highest neurological score appeared at 6 h after operation, descending significantly at sequential time. (2) Using TTC dyeing and optical microscope technique, pathological changes in brains were observed. (3) Compared with control group and sham operation groups, there was a decrease in tPA activity of model rats at the initial 12 h after injection of sodium laurate (P < 0.05), PAI activity decreased markedly in the model group at 24 h after injection of sodium laurate. (4) In plasma TXB(2) concentration reached the highest level compared at 6 h after injection of sodium laurate, but there were not obvious differences in plasma 6-keto-PGF(1alpha) concentration among all groups (P > 0.05). CONCLUSION: Focal cerebral infarction in rats could be induced by some sodium laurate, showing ischemic cerebrum necrosis, function disorder of vascular endothelium-platelet, fibrinolysis abnormality. This model could play an important role in researching the contribution of vascular endothelial cell during cerebral infarction development, preventing and curing by traditional Chinese medicine.
作者机构：
[付灵梅] Department of Integrated Traditional Chinese and Western Medicine, Hunan College of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China. flm1208@163.com
