关键词：
Apoptosis;Chemosensitivity;Lentivirus;Pancreatic cancer;Proliferation;RNA interference (RNAi);Survivin;X-linked apoptosis inhibiting protein (XIAP)
摘要：
At present, classic therapies provide limited benefits to the survival of patients with pancreatic cancer. However, clinically available gene therapy strategies have not been well established. This study investigates the effect of shRNA-mediated inhibition of XIAP and survivin expression on the proliferation, apoptosis, and chemosensitivity of pancreatic cancer cells. Stable inhibition of XIAP and survivin expression in SW1990 and Panc-1 pancreatic cancer cells was established by lentivirus-carried shRNAs. The mRNA and protein expression of XIAP and survivin were detected by real-time PCR and Western blot, respectively. Cell proliferation was measured by MTT assay, and apoptosis was detected by caspase-3/7 activity and Hoechst33342 staining. The lentivirus-carried shRNA significantly inhibited XIAP and survivin expression. Simultaneous inhibition of XIAP and survivin expression in pancreatic cells significantly reduced cell proliferation, increased caspase-3/7 activity, and increased cell sensitization to 5-FU and gemcitabine treatments compared to inhibition of XIAP or survivin expression alone. However, simultaneous silencing of XIAP and survivin showed no significant difference in inducing cell apoptosis compared to silencing of XIAP or survivin expression alone. Simultaneous inhibition of XIAP and survivin expression may be an effective strategy for gene therapy of pancreatic cancer.
摘要：
The objective of the study was to study the protective effect of Silybum marianum extract on hepatic ischemiareperfusion injury. Rats were randomly divided into five groups; namely Silybum marianum extract high-, medium-, and lowdose protection groups, model group and control group. Hepatic ischemia-reperfusion injury model was prepared. Serum or plasma AST, ALT, MDA, TNF-α, IL-1β, IL-6 levels were measured. The results revealed that after liver injury, AST, ALT, MDA, TNF-α, IL-1β, and IL-6 levels significantly increased in succession, showing significant differences. We concluded that inflammatory cytokines participate in liver injury and that Silybum marianum extract can reduce the production of inflammatory cytokines, and thus can have a protective effect on hepatic ischemia and reperfusion. Keywords : Silybum marianum , hepatic ischemia-reperfusion injury, protective effect
作者机构：
[Wu YiMou; Cao WenJuan; Zhou Zhou; Lu ChunXue; Zhou Hui; Li ZhongYu; Ma KangKang] Univ South China, Pathogen Biol Inst, Hengyang 421001, Peoples R China.;[Xie XiaoBing; Zhou Hui] Hunan Univ Chinese Med, Hosp 1, Dept Lab Med, Changsha 410007, Hunan, Peoples R China.;[Huang QiuLin] Univ South China, Affiliated Hosp 1, Dept Gen Surg, Hengyang 421001, Peoples R China.;[Zhong GuangMing] Univ Texas Hlth Sci Ctr San Antonio, Dept Microbiol & Immunol, San Antonio, TX 78229 USA.
通讯机构：
[Li ZhongYu] U;Univ South China, Pathogen Biol Inst, Hengyang 421001, Peoples R China.
关键词：
Chlamydia trachomatis;pORF5 plasmid protein;mitogen-activated protein kinase;proinflammatory cytokines;TLR2
摘要：
Infection with Chlamydia trachomatis induces inflammatory pathologies in the urogenital tract that can lead to infertility and ectopic pregnancy. Pathogenesis of infection has been mostly attributed to excessive cytokine production. However, precise mechanisms on how C. trachomatis triggers this production, and which protein(s) stimulate inflammatory cytokines remains unknown. In the present study, the C. trachomatis pORF5 protein induced tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interleukin-8 (IL-8) in dose- and time-dependent manners in the THP-1 human monocyte cell line. We found that intracellular p38/mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK)/MAPK signaling pathways were required for the induction of TNF-alpha, IL-1 beta and IL-8. Blockade of toll-like receptor 2 (TLR2) signaling reduced induction levels of TNF-alpha, IL-8 and IL-1 beta. We concluded that the C. trachomatis pORF5 protein might contribute to the inflammatory processes associated with chlamydial infections.
通讯机构：
[Liu, Shaojun] C;Cent S Univ, Xiang Ya Sch Med, Affiliated Hosp 3, Dept Digest Med, Changsha 410078, Hunan, Peoples R China.
关键词：
Folium Cordylines Fruticosae Anti-gastric Cancer MGC-803 cell
摘要：
The active components in Folium Cordylines Fruticosae were extracted by heat reflux method. The solvents used were distilled water and ethanol. The effects of two types of extracts on gastric cancer cells were compared; dry extract yields were calculated, as well as the inhibition rates of gastric cancer MGC-803 cell proliferation and the colony cell counts. The micro-Kjeldahl method was used to measure the cell protein contents and to make a comprehensive comparison. The results showed that the MGC-803 cell inhibition rates of three different concentrations (32.5, 75 and 150 mg/ml) of ethanol extracts increased with the increase of concentration, which was 48.9% at a concentration of 150 mg/ml; aqueous extract of Folium Cordylines Fruticosae had very low inhibitory activity at a low concentration (32.5 mg/ml), which was remained at about 20%. After being affected by two types of extracts, cells had uneven sizes, with very low brightness, while the normal cells presented a uniform full form, with high definition.
作者机构：
[Hu, Hua; Liu, Lijuan; Zhou, Desheng; Zhong, Jie; Chen, Yao] Hunan Univ Chinese Med, Hosp 1, Dept Neurol, Changsha 410007, Hunan, Peoples R China.;[Li, Mei] Pingxiang Hosp Tradit Chinese Med, Dept Internal Med, Pingxiang 337000, Jiangxi, Peoples R China.;[Yang, Yang] Fujian Univ Tradit Chinese Med, Peoples Hosp, Dept Neurol, Fuzhou 350004, Fujian Province, Peoples R China.
通讯机构：
[Li, Mei] P;Pingxiang Hosp Tradit Chinese Med, Dept Internal Med, Pingxiang 337000, Jiangxi, Peoples R China.
关键词：
neural regeneration;traditional Chinese medicine;Huoxue Rongluo Tablet;cerebral infarction;neuroprotection;matrix metal oproteinase-9;buflomedil pyridoxal phosphate;grants-supported paper;neuroregeneration
摘要：
Huoxue Rongluo Tablet was made of tal gastrodis tuber, dahurian angelica root, honeysuckle stem, grassleaf sweetflag rhizome, common flowering quince fruit, figwort root, red peony root and peach seed at a ratio of 3:2:6:2:3:3:3:3. Huoxue Rongluo Tablet is a wel-established and common pre-scription for the treatment of cerebral infarction. In this study, a rat model of cerebral ischemia was established and the animals were intragastrical y administered Huoxue Rongluo Tablet. This treat-ment reduced infarct volume, decreased matrix metal oproteinase-9 expression, and improved neurological function. Moreover, the effects of Huoxue Rongluo Tablet were better than those of buflomedil pyridoxal phosphate. These results indicate that Huoxue Rongluo Tablet is effective in treating cerebral infarction by regulating matrix metal oproteinase-9 protein expression.
摘要：
One of our previous studies showed that Yizhijiannao Granule,a compound Chinese medicine, effectively improved the clinical symptoms of Alzheimer’s disease.In the present study,we established a model of Alzheimer’s disease using beta-amyloid(25-35)in PC12 cells,and treated the cells with Yizhijiannao Granule and its four monomers,i.e.,icariin,catechin,Panax notoginseng saponins,and eleutheroside E.Flow cytometry showed that Yizhijiannao Granule-containing serum, icariin,Panax notoginseng saponins,and icariin+Panax notoginseng saponins were protective against beta-amyloid(25-35)-induced injury in PC12 cells.Icariin in combination with Panax notoginseng saponins significantly inhibited early apoptosis of PC12 cells with beta-amyloid (25-35)-induced injury compared to icariin or Panax notoginseng saponins alone.The effects of icariin+Panax notoginseng saponins were similar to the effects of Yizhijiannao Granule.The findings indicate that two of the effective monomers of Yizhijiannao Granule,icariin and Panax notoginseng saponins,can synergistically inhibit early apoptosis of PC12 cells induced by beta-amyloid(25-35).
作者机构：
[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Wang, Chao-Run; Chen, Zhen; Deng, Gui-Ming] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Zhang, He-Ming] S;S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.
摘要：
In the title molecule, C 16H 14N 2O, the dihedral angle between the two phenyl rings is 23.5(6)°. In the crystal, N-H-O hydrogen bonds link molecules into chains running along the a axis.
摘要：
AIM: To explore the effect of Qingguangan on the expressions of MMP-2 and MMP-9 in filtering bleb scarring area after trabeculectomy in rabbit model. METHODS: Thirty-two New Zealand rabbits were randomized into four groups: control group, experimental group, MMC group (ocular trabeculectomy in combination with MMC), and Qingguangan group. Trabeculectomy was performed on both eyes in each group except control group. Qingguangan group was mouth-fed with Qingguangan (solution). On postoperative day 14, the appearances of MMP-2 and MMP-9 on filtrating blebs were observed by immunohistochemistry. RESULTS: Statistical differences of the expressions of MMP-2 and MMP-9 were noted among groups on day 14 following surgery. Histology immunohistochemistry showed significant differences on the expressions of MMP-2 and MMP-9 between each groups( P<0.05). CONCLUSION: Qingguangan can promote the expressions of MMP-2 and MMP-9.
作者机构：
[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Zhang, He-Ming; Deng, Gui-Ming] S China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Wang, Chao-Run; Chen, Zhen; Deng, Gui-Ming] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Zhang, He-Ming] S;S China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.
摘要：
The asymmetric unit of the title compound, C 8H 7N 3O 5, contains two independent molecules in which the amide plane is oriented at dihedral angles of 29.82(2) and 31.17(2)° with respect to the benzene ring. In the crystal, molecules are connected via intermolecular N-H··· O hydrogen bonds, forming chains running along the b axis.
摘要：
After gene mutation, the pcDNA3.1/APP595/596 plasmid was transfected into HEK293 cells to establish a cell model of Alzheimer's disease. The cell model was treated with donepezil or compound Danshen tablets after culture for 72 hours. Reverse transcription-PCR showed that the mRNA expression of amyloid protein precursor decreased in all groups following culture for 24 hours, and that there was no significant difference in the amount of decrease between donepezil and compound Danshen tablets. Our results suggest that compound Danshen tablets can reduce expression of the mRNA for amyloid protein precursor in a transgenic cell model of Alzheimer's disease, with similar effects to donepezil.
摘要：
At 8 weeks after intragastric administration of icariin to senescence-accelerated mice (P8 strain), Morris water maze results showed that escape latency was shortened, and the number of platform crossings was increased. Immunohistochemical staining and western blot assay detected significantly increased levels of cyclic adenosine monophosphate response element binding protein. These results suggest that icariin upregulates phosphorylated cyclic adenosine monophosphate response element binding protein levels and improves learning and memory functions in hippocampus of the senescence-accelerated mouse.
作者机构：
[Li, Guangcheng; Dong, Keli; Zhang, Ting; Zhu, Hong] Cent S Univ, Xiangya Hosp 3, Dept Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Zhang, Zhanwei] Hunan Univ Chinese Med, Hosp 1, Dept Neurosurg, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Dong, Keli] C;Cent S Univ, Xiangya Hosp 3, Dept Chinese Med, Changsha 410013, Hunan, Peoples R China.
关键词：
icariin;Alzheimer’s disease;entorhinal cortex;proteomics;traditional Chinese medicine;neural regeneration
摘要：
The present study sought to explore the mechanism of action by which icariin, an active component of Epimedii Herba, treats Alzheimer's disease at the proteomics level. Two-dimensional gel electrophoresis was used to isolate total protein from the entorhinal cortex of senescence-accelerated mouse prone 8 (SAMP8) mice, and differential protein spots were obtained. Corresponding peptide mass fingerprinting was conducted through mass spectrography to identify differential protein spots. Twenty-six differential protein spots were found in the entorhinal area of SAMP8 mice at 8 weeks following intragastric perfusion with icariin and double distilled water. Fourteen spots were identified, which were involved in mitochondrial energy metabolism, oxidative stress, and neuronal function. The results revealed that icariin can regulate the expression of various proteins in the entorhinal cortex of SAMP8 mice, and treat Alzheimer's disease by improving mitochondrial function, suppressing oxidative stress, inhibiting neural cell apoptosis, and protecting neurons.
摘要：
AIM: To evaluate the effects of the extract of Buddleja officinalis eye drops in basic tears secretory volume, tear film stability, expression of androgen receptors (AR) in castrated rats with dry eye, and to investigate the therapeutic effects of the extract of Buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS: Forty-five Wistar masculinity rats were divided at random into nine groups, including normal groups (A1, A2 and A3); model groups (B1, B2 and B3); therapy groups with extract of Buddleja officinalis eye drops (C1, C2 and C3). The "1" stood for being fed for 1 month, and "2" for 2 months, and "3" for 3 months. The dry eye model was established with orchiectomy on groups B and C. Group C was treated with Buddleja officinalis extract eye drops for one month. All rats were checked with Schirmer I test (SIT) and tear film break-up time (BUT). Expression of AR was analyzed by flow cytometer (FCM). RESULTS: The SIT value of group C was significantly higher than that of group B (P<0.01) and the BUT value of group C was significantly longer than that of group B (P<0.01), which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. And the expression of AR of group C was much higher than that of group B, which showed that available composition of the eye drops maybe display androgen-like activity. CONCLUSION: The main components of the extract of Buddleja officinalis is the flavonoids that can significantly inhibit happening of dry eye of rat after androgen level lowered. Its mechanism is like androgen's and it can display androgen-like activity to keep basic tears secretory volume and tear film stability.
摘要：
• AIM: To explore the effect of Buddleia flavonoids drugcontaining plasma and androgen receptor(AR) blocker on the expression of STAT1 phosphoprotein. • METHODS: In vitro lacrimal gland epithelial cells were cultivated with H2 O2 to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot and AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids. • RESULTS: After the intervention of drug-containing plasma, the expression of STAT1 Phosphoprotein in Buddleja officinalis drug-containing plasma intervention group ( 0. 353 ± 0. 494 ) and testosterone propionate intervention group (0.502 ± 0.036) were enhanced and the differences between the two groups were significant ( P < 0.01). After using the AR blocker in all groups, the expression of STAT1 phosphoprotein in each group (0. 268 ± 0. 061, 0. 283 ± 0. 106, 0. 213 ± 0. 071 ) had no difference. • CONCLUSION: Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.
通讯机构：
[Peng, Qing-Hua] H;Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Dept Ophthalmol, Changsha 410007, Hunan, Peoples R China.
关键词：
dry;eye;LACRIMAL;GLAND;extract;of;buddleja;OFFICINALIS;ANDROGEN;lacrimal gland;extract of buddleja officinalis;androgen
摘要：
AIM: To assess the effects of extract of Buddleja officinalis on tear secretion volume, tear film stability, expressions of TGF-beta 1, IL-1 beta, TNF-alpha in lacrimal gland of castrated rabbits with dry eye. METHODS: A total of 30 victory rabbits were divided averagely into normal group (A), model group (B), therapy group with low dose extract of Buddleja officinalis (C), therapy group with high dose extract of Buddleja officinalis (D) and therapy group with genistein (E). The dry eye model was established with orchiectomy on Group B, C, D, E. Group C, D, E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT. TGF-beta 1, IL-1 beta, TNF-alpha were detected with immunohistochemistry and the ultrastructure of lacrimal gland was observed under transmission electron microscope. RESULTS: The SIT value of group C, D, E were respectively 13.167 +/- 4.957, 14.667 +/- 5.279, 8.667 +/- 0.516, obviously higher than that of group B 5.667 +/- 2.338 (P<0.01). The positive expression of IL-1 beta in acinar cell and glandular tube cell of group C, D were 0.470 +/- 0.048, 0.510 +/- 0.088, obviously lower than that of group B 0.770 +/- 0.118 (P <0.01). The positive expression of TNF-alpha of group C, D were 0.498 +/- 0.156, 0.435 +/- 0.069, obviously lower than that of group B 0.769 +/- 0.095 too (P <0.01). The positive expression of TGF-beta 1 of group C, D were 0.406 +/- 0.171, 0.497 +/- 0.147, obviously higher than that of group B 0.222 +/- 0.113(P<0.01). Any result of group C, D was positive compared with that of group E (P <0.05). Ultrastructure of the lacrimal gland of group C, D, E was well preserved, especially in D group it was remarkable. CONCLUSION: The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.
作者机构：
[Song lan] Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, Changsha 410008, Hunan, Peoples R China.;[Xu Zhao-jun] Hunan Univ Tradit Chinese Med, Cardiothorac Surg Affiliated Hosp 1, Changsha 41007, Hunan, Peoples R China.;[Zhang Cai-ping; Tian ying] Nanhua Univ, Coll Life Sci, Dept Biochem & Mol Biol, Hengyang 421001, Hunan, Peoples R China.
通讯机构：
[Song, L ] ;Cent S Univ, Xiangya Sch Med, Dept Pathophysiol, 110 Xiangya Rd, Changsha 410008, Hunan, Peoples R China.
关键词：
fibroblast;adrenaline;bFGF;TGF-beta 1
摘要：
Adrenaline has been shown to modulate proliferation of mouse fibroblasts, adventitial fibroblasts and synovial B (fibroblasts-like) cells. However, little is known about the response of cultured human hypertrophic scar fibroblasts to adrenaline. In this study, we investigated cell proliferation and involved mechanisms in hypertrophic scar fibroblasts in response to adrenaline. Population doubling time (PDT) assay and MTT assay were performed to determine the cell proliferation and cell viability, respectively. The expression of bFGF and TGF-beta 1 was measured by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme linked immunosorbent assay (ELISA). The results showed that adrenaline inhibited proliferation of normal and hypertrophic scar fibroblasts in a dose-dependent manner. Moreover, adrenaline up-regulated the expression of bFGF and down-regulated the expression of TGF-beta 1 in normal and hypertrophic scar fibroblasts. Interestingly incubation with the a receptor antagonist regitine indicated that adrenaline mediated inhibition of cell proliferation and regulation of TGF-beta 1 and bFGF in cultured normal and hypertrophic scar fibroblasts were mediated by the a receptor. These studies suggest that adrenaline inhibits proliferation and alters the expression of TGF-beta 1 and bFGF in human hypertrophic scar fibroblast involving an a receptor mediated pathway.
