作者：
Zheng, H.;Liu, Z. -S.;Zhang, W.;Chen, M.;Zhong, F.;...
期刊：
NEUROGASTROENTEROLOGY AND MOTILITY,2018年30(7):e13307- ISSN：1350-1925
通讯作者：
Li, Y.;Zhu, B.
作者机构：
[Li, Y.; Zheng, H.] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Li, Y.; Zhu, W. -Z.; Liu, Z. -S.] China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China.;[Zhong, F.; Zhang, W.] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Chen, M.] Chengdu Univ Tradit Chinese Med, Teaching Hosp, Chengdu, Sichuan, Peoples R China.;[Rong, P. -J.; Zhu, B.; Jing, X. -H.] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
通讯机构：
[Li, Y.; Zhu, B.] C;Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
期刊：
American Journal of Obstetrics and Gynecology,2018年219(4):373.e1-373.e10 ISSN：0002-9378
通讯作者：
Liu, Baoyan
作者机构：
[Liu, Baoyan; Wang, Yang; Zhou, Jing; Yang, Likun; Liu, Zhishun; Wu, Jiani; Wang, Weiming] China Acad Chinese Med Sci, Guangan Men Hosp, Beijing, Peoples R China.;[He, Liyun; Yan, Shiyan; Ai, Yanke; Liu, Jia; Liu, Yan] China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing, Peoples R China.;[Xu, Huanfang] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.;[Zhou, Kehua] Univ Buffalo, Catholic Hlth Syst Internal Med Training Program, Buffalo, NY USA.;[Dong, Guirong] Shanghai Univ Tradit Chinese Med, Yueyang Hosp Integrated Tradit Chinese & Western, Shanghai, Peoples R China.
通讯机构：
[Liu, Baoyan] C;China Acad Chinese Med Sci, Guangan Men Hosp, Beijing, Peoples R China.
关键词：
acupuncture;hot flash;menopause transition;Menopause Rating Scale;Menopause-Specific Quality of Life
摘要：
Background: Auditory Neuropathy Spectrum Disorder (ANSD) is manifested as impairment of auditory nerve activity but preservation of the outer hair cell function. Objective: This study was to detect the disease-causing gene and variant(s) in a Chinese ANSD family. Methods: A four-generation consanguineous Chinese ANSD family and 200 unrelated healthy controls were enrolled. Exome sequencing and Sanger sequencing were applied to identify the genetic basis for ANSD in this family. Results: Exome sequencing detected a c.1236delC variant of the otoferlin gene in an apparently homozygous state. Sanger sequencing confirmed that the variant co-segregating with the phenotype of hearing impairments in this family. The variant was not detected in 200 healthy controls. The c.1236delC alteration may result in a truncated otoferlin missing the C2C-C2F domains and the Cterminal transmembrane domain, and thus severely damages Ca2+-dependent synaptic vesicle fusion and targeting function of the otoferlin. Conclusion: Our study suggested that the c.1236delC alteration in the otoferlin gene may be the diseasecausing variant in this family, and also shed new light on genetic counseling to this ANSD family.
期刊：
European Journal of Gastroenterology and Hepatology,2018年30(2):130-135 ISSN：0954-691X
通讯作者：
Sun, Kewei;Lang, Ren
作者机构：
[Sun, Kewei; Wang, Ya; Zhang, Tao] Hu Nan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Huang, Lei] 302 Hosp PLA, Treatment & Res Ctr Infect Dis, Beijing, Peoples R China.;[Lang, Ren] Capital Med Univ, Dept Hepatobiliary Surg, Beijing Chaoyang Hosp, Beijing 10020, Peoples R China.;[Jiang, Wei; Zhang, Tao] Med Univ South Carolina, Dept Microbiol & Immunologys, Div Infect Dis, Charleston, SC USA.;[Jiang, Wei] Med Univ South Carolina, Dept Med, Div Infect Dis, Charleston, SC USA.
通讯机构：
[Sun, Kewei] H;[Lang, Ren] C;Hu Nan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;Capital Med Univ, Dept Hepatobiliary Surg, Beijing Chaoyang Hosp, Beijing 10020, Peoples R China.
摘要：
Acute-on-chronic liver failure (ACLF) is characterized by organ failure mediated by acute decompensation of cirrhosis. Recent studies have highlighted the importance of the gut-liver axis (GLS) and its association with ACLF pathogenesis. In this review, we discuss the mechanisms related to the alteration of the GLA and their involvement in ACLF pathogenesis and suggest some possible therapeutic options that could modulate the GLA dysfunction. This knowledge may provide information useful for the design of therapeutic strategies for gut dysbiosis and its complications in ACLF. Copyright (C) 2018 Wolters Kluwer Health, Inc. All rights reserved.
期刊：
Evidence-Based Complementary and Alternative Medicine,2018年2018:2901863 ISSN：1741-427X
通讯作者：
Wang, Yuhong
作者机构：
[Li, Wei; Liu, Jian; Yang, Hui] Hunan Univ Chinese Med, Affiliated Hosp 1, Pharmacol Res Ctr, Changsha, Hunan, Peoples R China.;[Yang, Hui] Hunan Univ Chinese Med, Domest First Class Construct Discipline Chinese M, Changsha, Hunan, Peoples R China.;[Meng, Pan; Wang, Yuhong; Liu, Zhuo] Hunan Univ Chinese Med, Key Lab Chinese Mat Med Power & Innovat Drugs Est, Changsha, Hunan, Peoples R China.;[Wang, Yuhong] Hunan Univ Chinese Med, Inst Innovat & Appl Res, Changsha, Hunan, Peoples R China.
通讯机构：
[Wang, Yuhong] H;Hunan Univ Chinese Med, Key Lab Chinese Mat Med Power & Innovat Drugs Est, Changsha, Hunan, Peoples R China.;Hunan Univ Chinese Med, Inst Innovat & Appl Res, Changsha, Hunan, Peoples R China.
摘要：
Diabetes-induced brain insulin resistance is associated with many mental diseases, including depression. Epidemiological evidences demonstrate the pathophysiologic link between stress, depression, and diabetes. This study was designed to determine whether chronic unpredictable mild stress- (CUMS-) induced changes in brain insulin resistance could contribute to deterioration in mood and cognitive functions in diabetic rats. Male SD rats were randomly assigned to three groups, including standard control group, the diabetes group, and the diabetes with CUMS group. After 7 weeks, emotional behaviors and memory performances as well as metabolic phenotypes were measured. In addition, we examined the changes in protein expression related to brain insulin signaling. Our results show that rats in diabetes with CUMS group displayed a decreased locomotor behavior in open-field test, an increased immobility time in forced swim test, and tail suspension test, and an impaired learning and memory in the Morris water maze when compared to animals in diabetes group. Further, diabetes with CUMS exhibited a significant decrease in phosphorylation of insulin receptor and an increase phosphorylation of IRS-1 in brain. These results suggest that the depression-like behaviors and cognitive function impairments in diabetic rats with CUMS were related to the changes of brain insulin signaling.
作者：
Guo, J.;Zhou, Q.;Zhang, R.;Zhang, C. H.;Xuan, Z. H.;...
期刊：
Journal of Sexual Medicine,2018年15(SUPPL-3):S189-S189 ISSN：1743-6095
作者机构：
[Zhang, X. J.; Gao, Q. H.; Wang, F.; Liu, Y.; Guo, J.; Yan, B.; Qiu, J. F.] China Acad Chinese Med Sci, Xiyuan Hosp, Beijing, Peoples R China.;[Zhou, Q.] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Zhang, R.] Heilongjiang Univ Chinese Med, Hosp 2, Harbin, Heilongjiang, Peoples R China.;[Zhang, C. H.] Yunnan Prov Hosp Tradit Chinese Med, Kunming, Yunnan, Peoples R China.;[Xuan, Z. H.] Beijing Chinese Med Hosp, Shunyi Hosp, Beijing, Peoples R China.
作者机构：
[Cai, Xiong; Yu, Rong; Song, Hou-Pan; Li, Ru-Yi; Huang, Hui-Yong; Li, Xin] Hunan Univ Chinese Med, Hunan Prov Key Lab Diagnost & Therapeut Res Chine, Changsha 410208, Hunan, Peoples R China.;[Hou, Xue-Qin] Taishan Med Coll, Inst Pharmacol, Tai An 271000, Shandong, Peoples R China.;[Zhou, Sai-Nan] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Cai, Xiong] Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Guangzhou 510006, Guangdong, Peoples R China.;[Zhou, Chi] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Guangdong, Peoples R China.
通讯机构：
[Zhou, Chi; Cai, Xiong] G;Guangzhou Univ Chinese Med, Affiliated Hosp 1, Guangzhou 510405, Guangdong, Peoples R China.;Guangdong Pharmaceut Univ, Sch Chinese Mat Med, Higher Educ Mega Ctr, 280 East Waihuan Rd, Guangzhou 510006, Guangdong, Peoples R China.
关键词：
Atractylenolide I;Cell migration and proliferation;Cytosolic free Ca(2+);Intestinal epithelial repair;Polyamine
摘要：
Background : An impairment of the integrity of the mucosal epithelial barrier can be observed in the course of various gastrointestinal diseases. The migration and proliferation of the intestinal epithelial (IEC-6) cells are essential repair modalities to the healing of mucosal ulcers and wounds. Atractylenolide I (AT-I), one of the major bioactive components in the rhizome of Atractylodes macrocephala Koidz. (AMR), possesses multiple pharmacological activities. This study was designed to investigate the therapeutic effects and the underlying molecular mechanisms of AT-I on gastrointestinal mucosal injury. Methods: Scratch method with a gel-loading microtip was used to detect IEC-6 cell migration. The real-time cell analyzer (RTCA) system was adopted to evaluate IEC-6 cell proliferation. Intracellular polyamines content was determined using high performance liquid chromatography (HPLC). Flow cytometry was used to measure cytosolic free Ca2+ concentration ([Ca2+](c)). mRNA and protein expression of TRPC1 and PLC-gamma(1) were determined by real-time PCR and Western blotting assay respectively. Results: Treatment of IEC-6 cells with AT-I promoted cell migration and proliferation, increased polyamines content, raised cytosolic free Ca2+ concentration ([Ca2+] c), and enhanced TRPC1 and PLC-gamma(1) mRNA and protein expression. Depletion of cellular polyamines by DL-a-difluoromethylornithine (DFMO, an inhibitor of polyamine synthesis) suppressed cell migration and proliferation, decreased polyamines content, and reduced [Ca2+] c, which was paralleled by a decrease in TRPC1 and PLC-gamma(1) mRNA and protein expression in IEC-6 cells. AT-I reversed the effects of DFMO on polyamines content, [Ca-2+] c, TRPC1 and PLC-gamma(1) mRNA and protein expression, and restored IEC-6 cell migration and proliferation to near normal levels. Conclusion: Our data demonstrate that AT-I stimulates intestinal epithelial cell migration and proliferation via the polyamine-mediated Ca2+ signaling pathway. Therefore, AT-I may have the potential to be further developed as a promising therapeutic agent to treat diseases associated with gastrointestinal mucosal injury, such as inflammatory bowel disease and peptic ulcer. (C) 2017 Elsevier GmbH. All rights reserved.
摘要：
Novel organobismuth(III) complex of 5H-dibenzo[c,f][1,5]oxabismocin-12(7H)-yl nitrate (C2) was synthesized and characterized by spectral and elemental analysis. It was compared with other five C,E,C-chelating (E = N, O, S) organobismuth(III) complexes against human adenocarcinoma alveolar basal epithelial cells (A549), human liver cancer cell line (SMCC7721), human gastric cancer cell line (SGC-7901), human colon adenocarcinoma cell line (SW480) and healthy human bronchial cell line (16HBE14o-) in vitro. It was found that C2 exhibited the best anticancer activity. Further mechanistic investigation indicated that toxicological activity of C2 was ascribable to apoptosis rather than anti-proliferative activity. Apoptosis was induced through up-regulating the level of Bcl-2/Bax as well as the activation of caspase-3. The results demonstrate that heterocyclic organobismuth(III) complexes of this type have great potential in the treatment of cancer.
摘要：
Traditional Chinese medicine has great potential to improve wound healing. ANBP, the mixture of 4 Chinese herbs—Agrimoniapilosa, Nelumbonucifera, Boswelliacarteri, and Pollen typhae—is effective in trauma treatment while its mechanism is still elusive. In this study, quantitative proteomics and bioinformatics analyses were performed to decipher the possible roles of ANBP in accelerated wound healing of mouse skin. Among all 3171 identified proteins, 90, 71, 80, and 140 proteins were found to be differently expressed in 6 hours, 3 days, 7 days, and 14 days ANBP-treated tissues compared with corresponding control tissues, respectively. The result showed that different biological processes and pathways were activated at different healing stages. At the early healing stage, ANBP treatment mainly affected several biological processes, including immune and defense response, vascular system restoration, hemostasis and coagulation regulation, lipid metabolism and signal transduction, while muscle tissue, hair, epidermis, extracellular matrix and tissue remodeling related activities were the major events in ANBP promoted later wound healing. This is the first quantitative proteome study of ANBP-treated wound tissues, which provide a new perspective for the mechanism of ANBP accelerated wound healing and is of guiding significance for clinical application of ANBP in trauma disorders cure.
