关键词：
hepatitis B virus;interferon;mutation;treatment efficacy
摘要：
BACKGROUND: Accumulating studies assessing the impacts of hot spot mutations on conventional interferon (IFN) efficacy come to discrepant conclusions; studies regarding the mutations in S and RT regions are also unclear. The present study aimed to evaluate the impacts of HBV mutations on the efficacy of conventional IFN. METHODS: A total of 126 patients who received conventional IFN treatment for 48 weeks were enrolled. Biochemical and serological parameters were routinely tested. The sequences of HBV from 78 serum samples were amplified by nested-PCR; mutations were identified with sequence scanner V1.0 after ABI 3730xl direct sequencing, HBV genotypes were determined according to RT gene sequences utilizing NCBI Genotyping Tool which was based on phylogenetic analysis. RESULTS: The baseline DNA levels of virological response (VR) group were significantly lower than those of no VR group [7.13±0.76 vs 7.69±0.56 lg (copies/mL), P=0.001]. The baseline ALT levels were significantly higher in the HBeAg clearance group (204.72±88.65 vs 162.80±85.81 IU/L, P<0.05) and HBeAg seroconversion group (204.89 ±95.68 vs 166.75±84.43 IU/L, P<0.05). Females and lower BMI levels (20.01±2.33 vs 21.65±3.66 kg/m~2, P<0.05) were prone to acquired biochemical response (BR). PC-W28STOP (ntG1896A) was significantly higher in the combined response (CR) group than that in the no CR group (91.7% vs 39.7%, P=0.001). Multivariate logistic regression analysis showed that baseline DNA, PC-P159T (nt- C2288A), BCP-N118T (ntA1726C) and BCP-L134L (ntA1775C/ G/T) influenced VR independently. PC-G182C (ntG2357T), PC-S64A/T (ntT2003G/A) and BMI were independent influence factors for HBeAg clearance, HBeAg seroconversion and BR, respectively. The new predicting model concluded that baseline DNA and new mutations for VR were established successfully, and ROC analysis showed that AUC was 0.842 (P<0.001) with a sensitivity of 0.652 and a specificity of 0.933. CONCLUSIONS: PC-P159T (ntC2288A), BCP-N118T (ntA1726C), BCP-L134L (ntA1775C/G/T), PC-G182C (ntG2357T) and PCS64A/ T (ntT2003G/A) were novel identified mutations that impacted IFN therapeutic efficacy. These novel mutations could serve as important predictors before conventional IFN treatment.
作者机构：
[Weng, Guohu; Pan, Suyue] Southern Med Univ, Nanfang Hosp, Dept Neurol, Guangzhou, Guangdong, Peoples R China.;[Huang, Shixiong; Wen, Guoqiang; Weng, Guohu] Peoples Hosp Hainan Prov, Dept Neurol, Haikou, Hainan, Peoples R China.;[Zhou, Bo] Human Univ Chinese Med, Affiliated Hosp 1, Dept Intens Care Unit, Changsha, Hunan, Peoples R China.
通讯机构：
[Pan, Suyue] S;Southern Med Univ, Nanfang Hosp, Dept Neurol, Guangzhou, Guangdong, Peoples R China.
关键词：
Antitumor;Octahedral;Schiff base
摘要：
By using two flexible Schiff bases H2L1 and H2L2, two new Ni(II)-coordination compound, namely, Py3NiL1 (1) and Py3NiL2 (2) (Py = pyridine, L-1=3,5-Cl2C6H2(O) C=NC6H3(O)-4-Cl, L-2=3,5-Cl2C6H2(O) C=NC6H3(O)-4-NO2) have been synthesized under solvothermal conditions. Single crystal X-ray structural analysis reveals that compounds 1 and 2 are both six-coordinate in a distorted octahedral geometry. For 1, the 1D chain-like structure was observed by the C-H center dot center dot center dot pi interaction, and the 1D ribbon-like structure was formed by the C-H center dot center dot center dot pi and pi center dot center dot center dot pi interaction; for 2, the 1D ribbon-like structure was formed by the C-H center dot center dot center dot O hydrogen bonding interaction, and the pi center dot center dot center dot pi interaction on the side of 1D ribbon-like structure also result in the formation of a 2D structure. The in vitro antitumor activities of 1, 2 and their corresponding organic ligands Py, L-1 and L-2 were studied and evaluated, in which three human glioma cells (U373, U251 and SHG44) were used in the screening tests.
作者：
Luo Xiaodong*;Wang Peng;Li Zhe;Liang De;Huang Feng;...
期刊：
中医杂志,2017年37(2):193-200 ISSN：1001-1668
通讯作者：
Luo Xiaodong
作者机构：
[Luo Xiaodong; Li Zhe] Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Guangzhou 510370, Guangdong, Peoples R China.;[Wang Peng] Macau Univ Sci & Technol, Fac Chinese Med, Macau 999078, Peoples R China.;[Huang Feng; Liang De] Guangzhou Univ Chinese Med, Affiliated Hosp 1, Dept Orthopaed, Guangzhou 510405, Guangdong, Peoples R China.;[Liu Jinwen] Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Orthopaed, Guangzhou 510120, Guangdong, Peoples R China.;[Chen Xinyu] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Cardiol, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Luo Xiaodong] G;Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Encephalopathy, Guangzhou 510370, Guangdong, Peoples R China.
关键词：
Spondylosis;Deficiency of liver and kidney;Qi-deficiency blood stasis;Jingfukang granules;Randomized controlled trial
摘要：
OBJECTIVE: To evaluate the effectiveness and safety of cervical spondylosis formula granules in reducing the symptoms of patients with the nerve root type and the vertebral artery type of cervical spondylosis. METHODS: This was a multicenter, single-blind, randomized, controlled trial. From April 2002 to November 2003, 499 patients were randomly assigned to either the treatment or the control group. The treatment group was orally administered granules prepared with a formula for cervical spondylosis, while the control group was given Jingfukang granules. The treatment course was 1 month for both groups. RESULTS: In patients with the nerve root type of cervical spondylosis, the total effect rate in the treatment group (87.21% ) was significantly higher than that in the control group (80.70%, P < 0.01). After the treatment period in both groups, the treatment group had a significantly greater rate of resolution of pain, numbness of the upper limbs, muscle strength of the upper limbs, and fatigue than the control group (all P < 0.05). In patients with the vertebral artery type of cervical spondylosis, the total effect rate in the treatment group (82.07%) was similar to that in the control group (71.21% , P > 0.05). After the treatment period in both groups, the treatment group had a significantly greater rate of resolution of weakness of the waist and knees than the control group (P < 0.05). CONCLUSION: The cervical spondylosis formula granules significantly improve numbness, muscle strength, and fatigue, and reduce pain in patients with the nerve root type of cervical spondylosis, and improve the weakness of the waist and knees in patients with the vertebral artery type of cervical spondylosis.
期刊：
Journal of Leukocyte Biology,2017年102(6):1481-1486 ISSN：0741-5400
通讯作者：
Jiang, Wei;Huang, Lei
作者机构：
[Jiang, Wei; Zhou, Zejun; Luo, Zhenwu; Ogunrinde, Elizabeth; Wan, Zhuang; Zhang, Tao] Med Univ South Carolina, Dept Microbiol & Immunol, 173 Ashley Ave,BSB208D,9383, Charleston, SC 29466 USA.;[Martin, Lisa] Med Univ South Carolina, Dept Med, Div Infect Dis, Charleston, SC USA.;[Jiang, Wei; Zhang, Tao] Hu Nan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Li, Zhen; Wu, Hao] Capital Med Univ, Beijing Youan Hosp, Beijing, Peoples R China.;[Qin, Zhiqiang] Louisiana State Univ, Hlth Sci Ctr, Louisiana Canc Res Ctr, Dept Genet, New Orleans, LA USA.
通讯机构：
[Jiang, Wei] M;[Huang, Lei] P;Med Univ South Carolina, Dept Microbiol & Immunol, 173 Ashley Ave,BSB208D,9383, Charleston, SC 29466 USA.;Peoples Liberat Army 302 Hosp, Treatment & Res Ctr Infect Dis, Beijing 100039, Peoples R China.
关键词：
disease;autoantibody;HIV infection;ART
摘要：
Although effective antiretroviral therapy (ART) suppresses HIV viral replication, prevents AIDS-related complications, and prolongs life, a proportion of patients fails to restore the patients' CD4(+) T cell number to the level of healthy individuals. Increased mortality and morbidity have been observed in these patients. In the current study, we have investigated the role of auto-IgGs in CD4(+) T cell apoptosis and recovery in a cross-sectional study. All HIV(+) subjects were on viral-suppressive ART treatment with a different degree of CD4(+) T cell reconstitution. Total auto-IgG binding on CD4(+) T cell surfaces and its associated apoptosis and CD4(+) T cell recovery were analyzed by flow cytometry ex vivo. Total IgGs from plasma were tested for their binding capacities to CD4(+) T cell surfaces and their mediation to CD4(+) T cell death through NK cell cytotoxicity in vitro. HIV(+) subjects had increased surface binding of auto-IgGs on CD4(+) T cells compared with healthy controls, and IgG binding was associated with elevated CD4(+) T cell apoptosis in HIV(+) subjects but not in healthy controls. Plasma IgGs from HIV(+) subjects bound to CD4(+) T cells and induced cell apoptosis through NK cytotoxicity in vitro. Soluble CD4 (sCD4) preincubation prevented NK cell-mediated CD4(+) T cell death. Our results suggest that plasma autoantibodies may play a role in some HIV(+) patients with poor CD4(+) T cell recovery under viral-suppressive ART.
作者：
Huang, Y. Y.;Li, N.;Zhou, S. N.;Huang, Z. T.;Zhuang, Z. F.*
期刊：
Applied Physics B: Lasers and Optics,2017年123(9):1-7 ISSN：0946-2171
通讯作者：
Zhuang, Z. F.
作者机构：
[Zhuang, Z. F.; Huang, Y. Y.; Li, N.] South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;[Zhuang, Z. F.; Huang, Y. Y.; Li, N.] South China Normal Univ, Coll Biophoton, Lab Photon Chinese Med, Guangzhou 510631, Guangdong, Peoples R China.;[Huang, Y. Y.] South China Normal Univ, Lab Photon Informat Technol, Guangzhou 510631, Guangdong, Peoples R China.;[Zhou, S. N.] Hunan Univ Chinese Med, Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Huang, Z. T.] Guzhen Prod Promot Ctr, Zhongshan, Peoples R China.
通讯机构：
[Zhuang, Z. F.] S;South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Guangdong, Peoples R China.;South China Normal Univ, Coll Biophoton, Lab Photon Chinese Med, Guangzhou 510631, Guangdong, Peoples R China.
摘要：
Oculocutaneous albinism (OCA) is a group of heterogeneous and autosomal recessive disorders characterized by a reduction or complete loss of melanin biosynthesis in melanocytes. OCA type 1 (OCA1) is the most severe and common form of OCA, and is caused by mutations in the tyrosinase gene (TYR). The present study aimed to identify the genetic cause of OCA1 in a four-generation consanguineous Chinese Han family. Complete physical examinations were performed and blood samples were collected from five members of the family and 100 unrelated healthy controls. Exome sequencing was conducted in the proband, followed by verification in other family members, using Sanger sequencing. Patients in the family presented with typical OCA1 features, including hypopigmentation of the skin and hair, and distinctive ocular changes. A homozygous missense variant, c.896G>A (p.R299H), in the TYR gene was identified in two patients, which co-segregated with disease in the family. This variant was not present in the 100 healthy controls. These results expand the number of mutations identified to be responsible for OCA1 in the Chinese Han population, and may have implications for genetic counseling and clinical management of the disease.
摘要：
Wnt5a, a secreted glycoprotein, belongs to the noncanonical Wnt family involved in a wide range of organism development and tissue homeostasis. Wnt5a and its signaling pathway can regulate fundamental cellular processes, including specification of cell fate, proliferation, and survival. Accumulating evidence indicates that Wnt5a exhibits dual effects on angiogenesis. The formation of new blood vessels derives from pre-existing vessels via canonical and non-canonical Wnt pathways, depending on cell types, receptors, downstream effectors, and microenvironment. Given that the regulation of angiogenesis has been implicated in many diseases, such as cancer, neovascular eye diseases, and cardiovascular diseases, these findings suggest that Wnt5a may be a potential target for the treatment of angiogenesis-related diseases.
作者机构：
[Bi, Xiaoying; Yao, Xinsheng; He, Xiaoyan; Ma, Long; Ma, Qingqing; Ma, Rui] Zunyi Med Univ, Dept Immunol, Res Ctr Med & Biol Innovat & Practice Base Grad S, Zunyi, Guizhou, Peoples R China.;[Wang, Xiaomei] Hunan Univ Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Yang, Jiezuan] Zhejiang Univ, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Diag & Treatment Infect Dis,Coll Me, Hangzhou, Zhejiang, Peoples R China.;[Shi, Bin] Zunyi Med Univ, Dept Lab Med, Zunyi, Guizhou, Peoples R China.
通讯机构：
[Yao, Xinsheng; Yang, Jiezuan] Z;Zunyi Med Univ, Dept Immunol, Res Ctr Med & Biol Innovat & Practice Base Grad S, Zunyi, Guizhou, Peoples R China.;Zhejiang Univ, Affiliated Hosp 1, Collaborat Innovat Ctr Diag & Treatment Infect Di, State Key Lab Diag & Treatment Infect Dis,Coll Me, Hangzhou, Zhejiang, Peoples R China.
关键词：
Recombinant vaccines;B cells;Vaccination and immunization;Amino acid sequence analysis;Sequence analysis;Protein sequencing;Hepatitis B virus;Immune response
摘要：
Immunization with recombinant HBV vaccine induces specific immune responses in human causing B lymphocytes to produce protective HBsAb, and to form memory B lymphocytes, thereby facilitating HBV immunity in the body. However, B lymphocytes heterogeneity and characteristics are not fully elucidated. In this study, we conducted high-throughput sequencing of BCR heavy chain CDR3 repertoires in 3 healthy volunteers before and after the third immunization with recombinant HBV vaccine. We used Roche 454 Genome Sequencer FLX system to perform a comparative analysis of IgM and IgG H chain CDR3 repertoires. First, we found that the diversity of IgG H chain CDR3 repertoires was 1/6 of IgM on average. Moreover, after the third immunization with HBV vaccine, the diversity of IgG H chain CDR3 repertoires was 1/26 of IgM on average. Second, we detected relatively high levels of HBsAbs in all the healthy volunteers after immunization with HBV vaccine. The volunteers shared a small number of CDR3 sequences before and after immunization, and among each other. However, we did not find completely identical BCR H chain CDR3 amino acid sequences in these volunteers. Lastly, after immunization with recombinant HBV vaccine, the volunteers showed high frequency of IgG H chain CDR3 amino acid sequences mostly resulting from rearrangements of IGHV, IGHD and IGHJ, suggesting that the mechanism of high frequency CDR3 generation might be associated with the maturation of IgG affinity (somatic hypermutation) during the recombinant HBV vaccine-induced B lymphocyte responses. This study identified the characteristics and changes of BCR CDR3 repertoires before and after immunization with HBV vaccine, and evaluated the performance of the sequencing technology for this application. Our findings provide a basis for further research in B lymphocyte generated HBsAb heterogeneity and monitoring the maintenance of memory B lymphocytes.
期刊：
Journal of Affective Disorders,2017年224:56-60 ISSN：0165-0327
通讯作者：
Wang, Yuhong
作者机构：
[Meng, Pan; Wang, Yuhong] Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Xiangzui Rd, Changsha 410208, Hunan, Peoples R China.;[Han, Yuanshan; Yang, Hui] Hunan Univ Chinese Med, Hosp 1, Cent Lab, Changsha 410007, Hunan, Peoples R China.
通讯机构：
[Wang, Yuhong] H;Hunan Univ Chinese Med, State Key Lab Chinese Med Powder & Med Innovat Hu, Xiangzui Rd, Changsha 410208, Hunan, Peoples R China.
关键词：
Diabetes;Depression;25-hydroxyvitamin D
摘要：
Background: Because of the absence of data on the direct association between vitamin D and depression in patients with diabetes, we examined the association between vitamin D state (assessed by 25-hydroxyvitamin D [ 25(OH) D]) and the prevalence of depression in adult patients with type 2 diabetes mellitus(T2DM). Method: Cross-sectional data were obtained from 2786 patients with T2DM recruited from a Chinese diabetes registry. Patients' records were reviewed to obtain data pertaining to age, sex, Body Mass Index (BMI), marital status, level of education, smoking status, duration of diabetes mellitus, use of insulin, and presence of additional illnesses. A multiple logistic regression analysis adjusted for potential confounders was used to assess independent associations between serum levels of 25 (OH) D and depression (defined by the Patient Health Questionnaire-9). Results: Using the PHQ-9 cutoff value of >= 10, 5.71% (159/2786; 95% CI: 4.85-6.57%) were considered to have depression. The serum 25(OH) D levels were significantly lower in diabetic patients with depression than those patients without depression [10.2(IQR, 7.6-15.2) ng/ml vs. 14.6(IQR, 10.7-19.8)ng/ml, respectively; P < 0.0001]. Multivariate logistic regression analysis considering traditional risk factors and other biomarkers showed an inverse relationship between serum 25 (OH) D levels and depression when serum 25 (OH) D were used as a continuous variable (OR, 0.84; 95% CI, 0.77-0.90; P < 0.001). Compared with the first quartile of serum 25 (OH) D levels, the second quartile OR for depression was 0.83 (95% CI, 0.75-0.92, P=0.012). For the third and fourth quartiles, it was 0.40 (95% CI, 0.33-0.52, P < 0.001) and 0.15 (95% CI, 0.08-0.22; P < 0.001), respectively. Conclusions: We observed a significant negative association between serum levels of 25 (OH) D and depression in Chinese patients with T2DM.
期刊：
BMC Complementary Medicine and Therapies,2017年17(1):1-11 ISSN：2662-7671
通讯作者：
Xia, Zhilan
作者机构：
[Zhong, Xiaohong; Liu, Dongbo; Xia, Zhilan; Xie, Ling] Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha, Hunan, Peoples R China.;[Liu, Dongbo; Xie, Ling] State Key Lab Subhlth Intervent Technol, Changsha, Hunan, Peoples R China.;[Liu, Lin] Hunan Univ Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Xia, Zhilan; Xie, Ling] Hunan Engn Res Ctr Edible Fungi, Changsha, Hunan, Peoples R China.
通讯机构：
[Xia, Zhilan] H;Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha, Hunan, Peoples R China.;Hunan Engn Res Ctr Edible Fungi, Changsha, Hunan, Peoples R China.
关键词：
Freeze-dried powder from Ganoderma lucidum mycelia;Rrecurrent oral ulcer;Rregulatory T cells;T-helper cells 17
摘要：
Conventional scientific studies had supported the use of polysaccharides and β-glucans from a number of fungi, including Ganoderma lucidum for the treatment of recurrent oral ulceration (ROU). Our aim of the present study was to evaluate whether freeze-dried powder from G. lucidum mycelia (FDPGLM) prevents ROU in rats. A Sprague-Dawley (SD) rat model with ROU was established by autoantigen injection. The ROU rats were treated with three different dosages of FDPGLM and prednisone acetate (PA), and their effects were evaluated according to the clinical therapeutic evaluation indices of ROU. High-dose FDPGLM induced significantly prolonged total intervals and a reduction in the number of ulcers and ulcer areas, thereby indicating that the treatment was effective in preventing ROU. Enzyme-linked immunosorbent assay (ELISA) showed that high-dose FDPGLM significantly enhanced the serum transforming growth factor-β1 (TGF-β1) levels, whereas reduced those of interleukin-6 (IL-6) and interleukin-17 (IL-17). Flow cytometry (FCM) showed that the proportion of CD4+ CD25+ Foxp3+ (forkhead box P3) regulatory T cells (Tregs) significantly increased by 1.5-fold in the high-dose FDPGLM group compared to that in the rat model group (P < 0.01). The application of middle- and high-dose FDPGLM also resulted in the upregulation of Foxp3 and downregulation of retinoid-related orphan receptor gamma t(RORγt) mRNA. High-dose FDPGLM possibly plays a role in ROU by promoting CD4+ CD25+ Foxp3+ Treg and inhibiting T helper cell 17 differentiation. This study also shows that FDPGLM may be potentially used as a complementary and alternative medicine treatment scheme for ROU.
作者机构：
[Zeng, Fang; Zhou, Si-Yuan; Li, Ying; Zheng, Hui] Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Coll, Chengdu, Sichuan, Peoples R China.;[Zhang, Wei] Hunan Univ Tradit Chinese Med, Hosp 1, Changsha, Hunan, Peoples R China.;[Zheng, Hua-Bin] Chengdu Univ Tradit Chinese Med, Teaching Hosp, Chengdu, Sichuan, Peoples R China.;[Zhang, Wei; Liu, Zhi-Shun] China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China.;[Zhu, Bing; Rong, Pei-Jing; Jing, Xiang-Hong] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
通讯机构：
[Liu, Zhi-Shun; Zhu, Bing] C;China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China.;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
摘要：
Diarrhea-predominant irritable bowel syndrome (IBS-D) and functional diarrhea (FD) are highly prevalent, and the effectiveness of acupuncture for managing IBS-D and FD is still unknown. The aim of this study was to compare the effectiveness of electroacupuncture with loperamide. It was a prospective, randomized, parallel group controlled trial. A total of 448 participants were randomly assigned to He electroacupuncture group (n = 113), Shu-Mu electroacupuncture group (n = 111), He-Shu-Mu electroacupuncture group (n = 112), or loperamide group (n = 112). Participants in the 3 acupuncture groups received 16 sessions of electroacupuncture during a 4-week treatment phase, whereas participants in the loperamide group received oral loperamide 2 mg thrice daily. The primary outcome was the change from baseline in stool frequency at the end of the 4-weeks treatment. The secondary outcomes were the Bristol scale, the MOS 36-item short form health survey (SF-36), the weekly average number of days with normal defecations and the proportion of adverse events. Stool frequency was significantly reduced at the end of the 4-week treatment in the 4 groups (mean change from baseline, 5.35 times/week). No significant difference was found between the 3 electroacupuncture groups and the loperamide group in the primary outcome (He vs. loperamide group [mean difference 0.6, 95% CI, –1.2 to 2.4]; Shu-Mu vs. loperamide group [0.4, 95% CI, –1.4 to 2.3]; He-Shu-Mu vs. loperamide group [0.0, 95% CI, –1.8 to 1.8]). Both electroacupuncture and loperamide significantly improved the mean score of Bristol scale and increased the weekly average number of days with normal defecations and the mean scores of SF-36; they were equivalent in these outcomes. However, the participants in electroacupuncture groups did not report fewer adverse events than those in the loperamide group. Similar results were found in a subgroup analysis of separating patients with IBS-D and FD patients. Electroacupuncture is equivalent to loperamide for reducing stool frequency in IBS-D and FD patients. Further studies on cost effectiveness of acupuncture are warranted.
摘要：
Ethnopharmacological relevance: Liuwei Dihaung decoction (LWDHT) is a well-known classic traditional Chinese medicine formula, consists of six herbs including Rehmannia glutinosa Libosch.(family: Scrophulariaceae), Cornus officinalis Sieb.(family: Cornaceae), Dioscorea opposite Thunb.(family: Dioscoreaceae), Alisma orientale(G. Samuelsson) Juz (family: Alismataceae), Poria cocos (Schw.) Wolf (family: Polyporaceae) and Paeonia suffruticosa Andrews (family: Paeoniaceae). It has been used in the treatment of many types of diseases with signs of deficiency of Yin in the kidneys in China clinically. This study is aimed at investigating the effect of Liuwei dihuang decoction on PI3K/Akt signaling pathway in liver of T2DM rats with insulin resistance. Materials and methods: T2DM model was induced in male Sprague-Dawley (SD) rats by high sugar and high fat diets combined with small dose of streptozocin (STZ) injection. The successful T2DM rats were randomly allocated three group-vehicle group, positive control group and Liuwei Dihuang decoction group. After 12-weeks treatment with distilled water, rosiglitazone and LWDHT by intragastric administration respectively, the rats were put to death in batches. The variance of fasting blood glucose (FBG) and fasting insulin (FINS) in serum were determined, the pathological changes of each rats' liver were observed by hematoxylin-eosin (HE) staining, the expression of insulin receptor substrate 2(IRS2), phosphatidylinositol 3-kinase (PI3K) and protein kinas B (Akt) involving the canonical PI3K/Akt signaling pathway were detected by Real-time fluorescent quantitative PCR (RT-PCR), and the expression level of IRS2, PI3K, Akt protein and phosphorylated IRS2, PI3K, Akt protein were evaluated by Western Blot. All the data were analyzed by SPSS 17.0. Results: Four weeks of treatment with LWDHT could significantly decrease the level of FBG and FINS in serum, improve the cellular morphology of liver, kidney, pancreas tissue, and the expression of IRS2, PI31<, Akt mRNA and phosphorylated IRS2, PI3K, Akt protein involved in the canonical PI31</Akt signaling pathway of T2DM rats in liver were significantly up-regulated, while the total IRS2, PI31<, and Akt protein had no obvious changes. Conclusions: The results suggest that Liuwei Dihuang decoction could intervene insulin resistance of T2DM, in part, through regulation of canonical PI3K/Akt signaling pathway of T2DM rats in liver. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
