作者： Cao, Yuan-Yuan;Li, Ke;Li, Ying;Tian, Xiao-Ting;Ba, Hui-Xue;...

期刊： Pharmaceutical Biology,2020年58(1):176-183 ISSN：1388-0209

通讯作者： Ke Li<&wdkj&>Xiao-Hui Li

作者机构： [Tian, Xiao-Ting; Ba, Hui-Xue; Cao, Yuan-Yuan; Li, Xiao-Hui] Cent South Univ, Xiangya Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410013, Peoples R China.;[Li, Ke] Hunan Acad Tradit Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Li, Ying] Cent South Univ, Xiangya Hosp 3, Dept Hlth Management, Changsha, Peoples R China.;[Li, Xiao-Hui] Hunan Key Lab Bioanal Complex Matrix Samples, Changsha, Peoples R China.;[Wang, Aiping] Univ South China, Nanhua Affiliated Hosp, Inst Clin Res, Hengyang, Peoples R China.

通讯机构： [Ke Li] H;[Xiao-Hui Li] D;Hunan Academy of Traditional Chinese Medicine, Hunan, China<&wdkj&>Department of Pharmacology, Xiangya School of Pharmaceutical Science, Central South University, Changsha, China<&wdkj&>Hunan Key Laboratory for Bioanalysis of Complex Matrix Samples, Changsha, China

关键词： cardiac fibrosis;extracellular regulated protein kinases;H9c2;heart-to-body weight ratio;Left ventricular systolic pressure;left ventricular/tibia length;phalloidin staining

摘要： Context: The pharmacological functions of Dendrobium candidum Wall. ex Lindl. (Orchidaceae) in cardiac hypertrophy remains unclear. Objective: To evaluate whether D. candidum aqueous extract (DCAE) can attenuate experimental cardiac hypertrophy. Materials and methods: Cardiac hypertrophy in SD rats was induced by subcutaneously injection of isoproterenol (2 mg/kg), once a day for ten days. Rats were gavaged with DCAE (0.13 and 0.78 g/kg) daily for one month. At the end of treatment, measurement of left ventricular systolic pressure (LVSP), heart-to-body weight ratio (HW/BW), left ventricular/tibia length (LV/TL), atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) levels, haematoxylin-eosin staining, and Masson’s trichrome staining were conducted. In cultured H9c2 cells, DCAE (2 mg/mL) and U0126 (10 μM) were added 2 h before the isoproterenol (10 μM) stimulus. Phalloidin staining was used to evaluate cellular hypertrophy. The mRNA expression of ANP and BNP was measured by qRT-PCR. The expression of p-ERK was determined by immunoblotting. Results: DCAE treatment significantly reduced the following indicators in vivo: (1) the LVSP (16%); (2) HW/BW (13%); (3) LV/TL (6%); (4) ANP (39%); (5) BNP (32%). In cultured H9c2 cells, phalloidin staining showed that DCAE relieved cellular hypertrophy (53% reduction). Furthermore, immunoblotting showed that DCAE can significantly inhibit p-ERK protein expression in vivo and in vitro (39% and 27% reduction, respectively). Discussion and conclusions: DCAE prevents cardiac hypertrophy via ERK signalling pathway and has the potential for treatment of cardiac hypertrophy. © 2020, © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

语种： 英文

作者： He, Ming-Chao;Shi, Zhe;Qin, Meng;Sha, Nan-Nan;Li, Yue;...

期刊： American Journal of Chinese Medicine,2020年48(3):559-577 ISSN：0192-415X

通讯作者： Wang, Yong-Jun;Zhang, Yan;Yuan, Ti-Fei

作者机构： [Wang, Yong-Jun; Sha, Nan-Nan; Shu, Bing; He, Ming-Chao; Sun, Yue-Li; Wang, YJ; Zhang, Yan; Li, Yue] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Spine Dis Res Inst, Room 908,Bldg 12,725 South Wanping Rd, Shanghai 200032, Peoples R China.;[Wang, Yong-Jun; Shu, Bing; He, Ming-Chao; Sun, Yue-Li; Li, Yue; Zhang, Yan] Minist Educ, Key Lab Theory & Therapy Muscles & Bones, Shanghai 200032, Peoples R China.;[Yuan, Ti-Fei; Shi, Zhe] Shanghai Jiao Tong Univ, Shanghai Mental Hlth Ctr, Shanghai Key Lab Psychot Disorders, Sch Med, Shanghai 201108, Peoples R China.;[Liao, Duan-Fang; Shi, Zhe] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha 410208, Peoples R China.;[Qin, Meng] Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.

通讯机构： [Wang, YJ; Zhang, Y; Yuan, Ti-Fei] S;Shanghai Univ Tradit Chinese Med, Longhua Hosp, Spine Dis Res Inst, Room 908,Bldg 12,725 South Wanping Rd, Shanghai 200032, Peoples R China.;Shanghai Jiao Tong Univ, Shanghai Mental Hlth Ctr, Shanghai Key Lab Psychot Disorders, Sch Med, Shanghai 201108, Peoples R China.

关键词： Depression;Microglia;Muscone;Neuroinflammation;Renin-Angiotensin System

摘要： Depression is partially caused by inflammation in the central nervous system. Early study demonstrated that musk, glandular secretion from male musk deer, exerted an antidepressant-like effect. The aim of this study was to investigate if muscone, a bioactive ingredient in musk, could ameliorate neuroinflammation and depressive-like behaviors as well as explore the potential action mechanism. Mice were intraperitoneally (i.p.) injected with muscone for 2 weeks prior to administration of lipopolysaccharides (LPS, 1mg/kg, i.p.). Pre-treatment with muscone reversed the LPS-induced decrease in body weight within 24h and ameliorated depressive-like behaviors shown by sucrose preference, tail suspension test, and forced swimming test. LPS-induced activation of microglial cells and elevation in expression of inflammatory cytokines including IL-1β, RANTES, and MCP-1 in the prefrontal cortex of mice were effectively abrogated by muscone, which significantly down-regulated expression of TLR4, MyD88, Caspase-1, NLRP3, renin, and Ang II. In addition, treatment of BV2 microglia cells with muscone markedly attenuated the LPS-induced rise in protein expression of TLR4, Ang II, and IL-1β. This study revealed that muscone could ameliorate LPS-induced depressive-like behaviors by repressing neuroinflammation in the prefrontal cortex of mice caused by its suppression on microglia activation and production of inflammatory cytokines via acting on TLR4 pathway and RAS cascade. © 2020 World Scientific Publishing Company.

语种： 英文

作者： Wu, Ping;Hu, Shi;Liang, Qin;Guo, Wang;Xia, Yang;...

期刊： International Journal of Polymeric Materials and Polymeric Biomaterials,2020年69(6):398-405 ISSN：1563-535X

通讯作者： Shuai, Cijun;Li, Yongmin

作者机构： [Wu, Ping] Xiangtan Univ, Sch Chem Engn, Dept Pharmaceut Engn, Xiangtan, Peoples R China.;[Hu, Shi; Xia, Yang; Liang, Qin; Wu, Ping] Xiangtan Univ, Key Lab Environm Friendly Chem & Applicat, Minist Educ, Xiangtan, Peoples R China.;[Guo, Wang; Shuai, Cijun] Cent S Univ, Coll Mech & Elect Engn, State Key Lab High Performance Complex Mfg, 932 South Lushan Rd, Changsha 410083, Hunan, Peoples R China.;[Shuai, Cijun] Jiangxi Univ Sci & Technol, Ganzhou, Peoples R China.;[Li, Yongmin] Hunan Acad Tradit Chinese Med Affiliated Hosp, Hunan Key Lab Chinese Med Oncol, Changsha, Peoples R China.

通讯机构： [Shuai, Cijun] C;[Li, Yongmin] H;Cent S Univ, Coll Mech & Elect Engn, State Key Lab High Performance Complex Mfg, 932 South Lushan Rd, Changsha 410083, Hunan, Peoples R China.;Hunan Acad Tradit Chinese Med Affiliated Hosp, 58 Lushan Rd, Changsha 410083, Hunan, Peoples R China.

关键词： Antibacterial activity;drug-sustained release;polymer scaffold;total alkaloids from Semen Strychnine

摘要： Total alkaloids from Semen Strychnine (TASS), as an antibacterial drug, was loaded into polyetheretherketone (PEEK)/polyglycolide acid (PGA) scaffolds fabricated via 3 D printing technology. It focused on studying microstructure, drug-release behavior, antibacterial activity and biocompatibility of scaffolds. Results indicated that TASS had a good physiochemical compatibility with polymer matrix, and a sustained release for longtime in TASS-PEEK/PGA scaffolds. Moreover, the scaffolds manifested antimicrobial activity against E. coli and S. aureus in antibacterial tests and superb biocompatibility in vitro cell culture. These suggested that TASS-PEEK/PGA scaffolds were able to efficiently sustain TASS delivery and were a promising implant for treating bacterial infection. © 2019, © 2019 Taylor & Francis Group, LLC.

语种： 英文

作者： Liu, Sisi;Qiao, Zhongquan;Wang, Xiaoming*;Zeng, Huijie;Li, Yongxin;...

期刊： Gene,2019年705:127-132 ISSN：0378-1119

通讯作者： Wang, Xiaoming

作者机构： [Chen, Yi; Zeng, Huijie; Cai, Neng; Liu, Sisi; Wang, Xiaoming; Li, Yongxin; Qiao, Zhongquan] Hunan Acad Forestry, Changsha 410004, Hunan, Peoples R China.;[Liu, Sisi] Hunan Univ Tradit Chinese Med, Changsha 410208, Hunan, Peoples R China.

通讯机构： [Wang, Xiaoming] H;Hunan Acad Forestry, Changsha 410004, Hunan, Peoples R China.

关键词： Codon usage bias;Lonicera macranthoides;Lonicerae Flos;Transcriptome

摘要： Lonicera macranthoides Hand. -Mazz. is an important medicinal and economical plant in China, however, the codon usage bias (CUB) in L. macranthoides genes is still unknown. In this study, L. macranthoides transcriptome sequencing has been completed, and codon usage patterns in 36,090 reconstructed genes from the L. macranthoides transcriptome were examined. The mean GC content and GC3 value is 44.9% and 43.1%, respectively, which indicates that nucleotide contents of L. macranthoides genome is somewhat AT rich, and its codon bias pattern tends to use A/T-ending codons. According to neutrality plot, ENC plot, PR2-Bias plot and correspondence analysis, we know that both compositional constraint under selection and mutation could affect the CUB in L. macranthoides, and the mutation is the most determinant factor. Meanwhile, gene expression levels can influence its codon usage pattern. Furthermore, we identified 29 optimal codons and most of them ended with A/U. The study will lay a foundation for future research on gene prediction, genetic engineering and molecular evolution in L. macranthoides.

语种： 英文

作者： Zheng, Hui;Li, Juan;Li, Ying;Zhao, Ling;Wu, Xi;...

期刊： JOURNAL OF CLINICAL HYPERTENSION,2019年21(3):412-420 ISSN：1524-6175

通讯作者： Liang, Fan-Rong

作者机构： [Zhao, Ling; Liang, Fan-Rong; Chen, Jie; Li, Ying; Zheng, Hui; Wu, Xi; Li, Xiang] Chengdu Univ Tradit Chinese Med, Teaching Hosp 3, Acupuncture & Tuina Sch, Chengdu, Sichuan, Peoples R China.;[Li, Juan] Chengdu Univ Tradit Chinese Med, Coll Hlth Preservat & Rehabil, Chengdu, Sichuan, Peoples R China.;[Huang, Yin-Lan] Ningxia Med Univ, Gen Hosp, Tradit Chinese Med Dept, Ningxia, Peoples R China.;[Liu, Mi; Chang, Xiao-Rong] Hunan Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Changsha, Hunan, Peoples R China.;[Cui, Jin] Guiyang Univ Chinese Med, Acupuncture & Tuina Sch, Guiyang, Guizhou, Peoples R China.

通讯机构： [Liang, Fan-Rong] C;Chengdu Univ Tradit Chinese Med TCM, Chengdu, Sichuan, Peoples R China.

关键词： acupuncture;blood pressure variability;mild hypertension;randomized controlled trial

摘要： Acupuncture may be beneficial for patients with mild hypertension, but the evidence is not convincing. We aimed to examine the effect of acupuncture on blood pressure (BP) reduction in patients with mild hypertension. We conducted a multicenter, single-blind, sham-controlled, randomized trial in eleven hospitals in China. The trial included 428 patients with systolic blood pressure (SBP) from 140 to 159 mm Hg and/or with diastolic blood pressure (DBP) from 90 to 99 mm Hg. The patients were randomly assigned to receive 18 sessions of affected meridian acupuncture (n = 107) or non-affected meridian acupuncture (n = 107) or sham acupuncture (n = 107) during 6 weeks, or to stay in a waiting-list control (n = 107). All patients received 24-hour ambulatory blood pressure monitoring at weeks 6, 9, and 12. We included 415 participants in the intention-to-treat analysis. The two acupuncture groups were pooled in the analysis, since they had no difference in all outcomes. SBP decreased at week 6 in acupuncture group vs sham acupuncture vs waiting-list group (7.2 +/- 11.0 mm Hg vs 4.1 +/- 11.5 mm Hg vs 4.1 +/- 13.2 mm Hg); acupuncture was not superior to sham acupuncture (mean difference 2.7 mm Hg, 95% CI 0.4 to 5.9, adjusted P = 0.103) or waiting-list control (2.9 mm Hg, 95% CI -0.2 to 6.0, adjusted P = 0.078). However, acupuncture was superior to sham acupuncture (3.3 mm Hg, 95% CI 0.2 to 6.3, adjusted P = 0.035) and waiting-list control (4.8 mm Hg, 95% CI 1.8 to 7.8, P < 0.001) at week 9. Acupuncture had a small effect size on the reduction of BP in patients with mild hypertension.

语种： 英文

作者： Zhao, Ling*;Li, Dehua;Zheng, Hui;Chang, Xiaorong;Cui, Jin;...

期刊： JAMA INTERNAL MEDICINE,2019年179(10):1388-1397 ISSN：2168-6106

通讯作者： Zhao, Ling;Liang, Fanrong

作者机构： [Liang, Fanrong; Zhao, Ling; Zhao, L; Li, Ying; Fan, Hailong; Zheng, Hui; Wu, Xi] Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, 37 Shier Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.;[Li, Dehua] Hosp Chengdu Univ Tradit Chinese Med, Dept Acupuncture, Chengdu, Sichuan, Peoples R China.;[Chang, Xiaorong] Hunan Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Changsha, Hunan, Peoples R China.;[Cui, Jin] Guiyang Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Guiyang, Guizhou, Peoples R China.;[Wang, Ruihui] Shaanxi Univ Chinese Med, Acupuncture & Tuina Sch, Xianyang, Shaanxi, Peoples R China.

通讯机构： [Zhao, L; Liang, FR] C;Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, 37 Shier Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.

关键词： angina pectoris;angina, stable;acupuncture therapy discipline;antianginal therapy;adjunctive therapy;acupuncture procedure;acupuncture points;meridians

摘要： Importance: The effects of acupuncture as adjunctive treatment to antianginal therapies for patients with chronic stable angina are uncertain. Objective: To investigate the efficacy and safety of acupuncture as adjunctive therapy to antianginal therapies in reducing frequency of angina attacks in patients with chronic stable angina. Design, Setting, and Participants: In this 20-week randomized clinical trial conducted in outpatient and inpatient settings at 5 clinical centers in China from October 10, 2012, to September 19, 2015, 404 participants were randomly assigned to receive acupuncture on the acupoints on the disease-affected meridian (DAM), receive acupuncture on the acupoints on the nonaffected meridian (NAM), receive sham acupuncture (SA), and receive no acupuncture (wait list [WL] group). Participants were 35 to 80 years of age with chronic stable angina based on the criteria of the American College of Cardiology and the American Heart Association, with angina occurring at least twice weekly. Statistical analysis was conducted from December 1, 2015, to July 30, 2016. Interventions: All participants in the 4 groups received antianginal therapies as recommended by the guidelines. Participants in the DAM, NAM, and SA groups received acupuncture treatment 3 times weekly for 4 weeks for a total of 12 sessions. Participants in the WL group did not receive acupuncture during the 16-week study period. Main Outcomes and Measures: Participants used diaries to record angina attacks. The primary outcome was the change in frequency of angina attacks every 4 weeks from baseline to week 16. Results: A total of 398 participants (253 women and 145 men; mean [SD] age, 62.6 [9.7] years) were included in the intention-to-treat analyses. Baseline characteristics were comparable across the 4 groups. Mean changes in frequency of angina attacks differed significantly among the 4 groups at 16 weeks: a greater reduction of angina attacks was observed in the DAM group vs the NAM group (difference, 4.07; 95% CI, 2.43-5.71; P <.001), in the DAM group vs the SA group (difference, 5.18; 95% CI, 3.54-6.81; P <.001), and in the DAM group vs the WL group (difference, 5.63 attacks; 95% CI, 3.99-7.27; P <.001). Conclusions and Relevance: Compared with acupuncture on the NAM, SA, or no acupuncture (WL), acupuncture on the DAM as adjunctive treatment to antianginal therapy showed superior benefits in alleviating angina. © 2019 American Medical Association. All rights reserved.

语种： 英文

作者： Hu, Yi;Cai, Xiong;Zhan, Yang;Yuan, Xiaomin;Liu, Tanbin;...

期刊： BMC Veterinary Research,2019年15(1):1-10 ISSN：1746-6148

通讯作者： Wang, Aibing;Guo, Shiyin

作者机构： [Yang, Lingchen; Liu, Wei; Wang, Aibing; Tan, Lei; Li, Yalan; Yuan, Xiaomin; Hu, Yi; Liu, Tanbin] Hunan Agr Univ HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, LAMFG, Changsha 410128, Hunan, Peoples R China.;[Cai, Xiong] Hunan Univ Chinese Med, Inst Innovat & Appl Res Chinese Med, Changsha 410208, Hunan, Peoples R China.;[Zhan, Yang; Wang, Naidong; Deng, Zhibang; Yang, Yi] HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, LFP, Changsha 410128, Hunan, Peoples R China.;[Zhang, Lijie] Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China.;[Zhang, Lijie] Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China.

通讯机构： [Wang, Aibing; Guo, Shiyin] H;Hunan Agr Univ HUNAU, Coll Vet Med, Key Lab Anim Vaccine & Prot Engn, LAMFG, Changsha 410128, Hunan, Peoples R China.;HUNAU, Coll Food Sci & Technol, Changsha 410128, Hunan, Peoples R China.

关键词： Porcine circovirus 2(PCV2);Truncated Rep protein;Infectious clone;Rescued viruses;Virus replication;PCV2 evolution

摘要： Background: Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-YiY-3-2-1 and IF-YiY-3-2-10. Subsequently, these infectious clones which have 1.1 copy of PCV2 genome of their corresponding strains were transfected into PK15 cells to obtain rescued viruses, respectively. Results: Though all of the three infectious clones could be rescued, the copy number and infectivity of these rescued viruses were significantly different, as analyzed by fluorescence quantitative PCR, Tissue culture infectious dose 50 (TCID50), and indirect immunofluorescence assay (IFA). Notably, whether the PCV2 copy number, viral titer or the infectivity of rescued viruses from infectious clone IF-YiY-3-2-3 was significantly less than those of its parental clones. Meanwhile, the spatial structure of the Rep protein from the IF-YiY-3-2-3 displayed an apparent truncation at the C-terminal. Conclusions: These findings therefore suggest that the Rep protein with truncated C-terminal would reduce virus replication and infectivity, and there might also exist both favorable and unfavorable mutations in the ORF1 of PCV2 in the process of its evolution. © 2019 The Author(s).

语种： 英文

作者： Bao, Tingting;Yang, Kailin;Long, Zhiyong;Zeng, Liuting*;Li, Yuehua*

期刊： Medical Science Monitor,2019年25:8152-8171 ISSN：1643-3750

通讯作者： Li, Yuehua;Zeng, Liuting

作者机构： [Bao, Tingting] Beijing Univ Chinese Med, Beijing, Peoples R China.;[Li, Yuehua; Bao, Tingting] China Acad Chinese Mec Sci, Xiyuan Hosp, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing Anzhen Hosp, Beijing, Peoples R China.;[Long, Zhiyong] Shantou Univ, Med Coll, Shantou, Guangdong, Peoples R China.

通讯机构： [Li, Yuehua] C;[Zeng, Liuting] H;China Acad Chinese Mec Sci, Xiyuan Hosp, Beijing, Peoples R China.;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.

关键词： Herbal Medicine;Medicine, Chinese Traditional;Osteoporosis;Pharmacology

摘要： Background: Osteoporosis is an important health problem worldwide. Siwu decoction (SWD) and its modification have a good clinical effect on osteoporosis. However, the molecular mechanism of SWD on osteoporosis has not been thoroughly explained. Material/Methods: A systematic pharmacological methodology was utilized to predict the active compounds and potential targets of SWD, collect the genes of osteoporosis and the known targets of SWD, and analyze the osteoporosis and SWD’s network. Results: Five networks were constructed and analyzed: (1) Osteoporosis genes’ protein–protein interaction (PPI) network; (2) Compound-compound target network of SWD; (3) SWD-osteoporosis PPI network; (4) Compound-known target network of SWD; and (5) SWD known target- osteoporosis PPI network. Several osteoporosis and treatment-related targets (eg.,. HSP90AB1, FGFR1, HRAS, GRB2, and PGF), clusters, biological processes, and signaling pathways (e.g., PI3K-Akt signaling pathway, insulin signaling pathway, MAPK signaling pathway and FoxO signaling pathway) were found. Conclusions: The therapeutic effect of SWD on osteoporosis may be achieved by interfering with the biological processes and signaling pathways related to the development of osteoporosis. © Med Sci Monit.

语种： 英文

作者： Zhang, Zhixue;He, Lei;Huang, Shucai;Fan, Lidan;Li, Yining;...

期刊： Frontiers in Psychiatry,2019年9:722 ISSN：1664-0640

通讯作者： Liu, Jun;Yang, Ru

作者机构： [Li, Yining; Yang, Ru; Zhang, Zhixue; He, Lei; Fan, Lidan; Liu, Jun; Yang, R] Cent South Univ, Second Xiangya Hosp, Dept Radiol, Changsha, Hunan, Peoples R China.;[Li, Ping; Zhang, Zhixue] Hunan Univ Chinese Med, Fist Hosp, Dept Radiol, Changsha, Hunan, Peoples R China.;[Huang, Shucai] Fourth Peoples Hosp Wuhu, Dept Psychiat, Wuhu, Peoples R China.;[Zhang, Jun] Hunan Judicial Police Acad, Changsha, Hunan, Peoples R China.

通讯机构： [Liu, J; Yang, R] C;Cent South Univ, Second Xiangya Hosp, Dept Radiol, Changsha, Hunan, Peoples R China.

关键词： addiction;gray matter volume;long-term abstinence;methamphetamine;voxel-based morphometry

摘要： Background: A large portion of previous studies that have demonstrated brain gray matter reduction in individuals who use methamphetamine (MA) have focused on short-term abstinence, but few studies have focused on the effects of long-term abstinence of methamphetamine on brain structures. Materials and Methods: Our study includes 40 healthy controls and 44 abstinent methamphetamine-dependent (AMD) subjects who have abstained for at least 14 months. For every AMD subject, the age when they first used MA, the total time of MA use, the frequency of MA use in the last month before abstinence, the duration of abstinence and the craving score were recorded. Here we used magnetic resonance imaging (MRI) to measure the gray matter volume (GMV) of each subject with voxel-based morphometry method. Two-sample t-test (AlphaSim corrected) was performed to obtain brain regions with different gray matter volume (GMV) between groups. In addition, partial correlation coefficients adjusted for age, years of education, smoking, and drinking were calculated in the AMD group to assess associations between the mean GMV values in significant clusters and variables of MA use and abstinence. Results: Compared with the healthy control group, AMD group showed increased gray matter volumes in the bilateral cerebellum and decreased volumes in the right calcarine and right cuneus. Moreover, GMV of left cerebellum are positively correlated with the duration of abstinence in the AMD group (p = 0.040, r = 0.626). Conclusions: The present study showed that the gray matter volume in some brain regions is abnormal in the AMD subjects with long-term abstinence. Changes in gray matter volume of visual and cognitive function regions suggested that these areas play important roles in the progress of MA addiction and abstinence. In addition, positive correlation between GMV of the left cerebellum crus and duration of abstinence suggested that prolonged abstinence is beneficial to cognitive function recovery.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Zou, Guoying;He, Junyu;Huang, Yiran;Ma, Guoan;...

期刊： Oncology Letters,2018年15(1):415-422 ISSN：1792-1074

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; Zou, Guoying; He, Junyu; Xu, Guofeng; Li, Yong] Hunan Prov Second Peoples Hosp, Dept Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; Zou, Guoying; He, Junyu; Huang, Yiran; Xu, Guofeng; Li, Yong] Hunan Univ Chinese Med, Clin Med Sch, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; Zou, Guoying; He, Junyu; Ma, Guoan; Yu, Ping] Cent S Univ, Sch Basic Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China.;[Ma, Guoan] Tumor Hosp Hunan Prov, Dept Hepatobiliary Dis, Changsha 410008, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Dept Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.

关键词： cell cycle;cell migration;cell proliferation;hepatoma carcinoma;short interfering RNA;sperm-associated antigen 9

摘要： The incidence and mortality rates of hepatocellular carcinoma (HCC) are higher in China compared with in other countries. Further research is required in order to improve the diagnosis and treatment of HCC. Sperm-associated antigen 9 (SPAG9) protein has been revealed to serve an important function in cancer progression; however, the underlying mechanisms remain to be elucidated. The present study investigated the expression level of SPAG9 in HCC tissues using quantitative-polymerase chain reaction, immunohistochemistry and western blotting, and the results demonstrated that SPAG9 was overexpressed in HCC tissues compared with the adjacent non-cancerous tissues. To explore the potential mechanisms underlying SPAG9 in HCC, the effect of SPAG9 on cell proliferation, cell cycle, migration and invasion capacities were investigated in the QGY HCC cell line by RNA interference. It was revealed that inhibition of SPAG9 mRNA in QGY cells significantly inhibited the expression level of SPAG9 compared with the control. Depletion of SPAG9 expression decreased cell proliferation (P<0.01) and increased the percentage of cells in the G1/G2 cell cycle phase. The percentage of cells in the S phase was decreased, and cell migration and invasion capabilities in vitro were reduced (P<0.01). In summary, the results of the present study suggested that SPAG9 was upregulated in HCC and may serve an important function in cancer cell proliferation, differentiation and invasion. Whether SPAG9 is a potential diagnostic marker and therapeutic target of human HCC requires additional study. © 2018, Spandidos Publications. All rights reserved.

语种： 英文

作者： Liang, Hao（梁昊）;Wu, Lan;Li, Yingchen;Zeng, Yidi;Hu, Zhixi（胡志希）;...

期刊： Medicine,2018年97(24):e10889 ISSN：0025-7974

通讯作者： Zhang, Qiuyan;Zhou, Xiaoqing（周小青）

作者机构： [Hu, Zhixi; Liang, Hao; Zhou, Xiaoqing; Li, Xinchun; Zhang, Qiuyan; Wu, Lan; Zeng, Yidi] Hunan Univ Chinese Med, Inst TCM Diagnost, Changsha, Hunan, Peoples R China.;[Hu, Zhixi; Liang, Hao; Zhou, Xiaoqing; Li, Xinchun; Zhang, Qiuyan; Wu, Lan; Zeng, Yidi] Hunan Univ Chinese Med, Hunan Prov Key Lab TCM Diagnost, Changsha, Hunan, Peoples R China.;[Li, Yingchen] Cent S Univ, Xiangya Hosp 3, Changsha, Hunan, Peoples R China.;[Li, Yingchen] Hunan Inst Tradit Chinese Med, Affiliated Hosp, Changsha, Hunan, Peoples R China.;[Sun, Xiang] Hosp Changsha, Cardiol Dept, Changsha, Hunan, Peoples R China.

通讯机构： [Zhang, QY; Zhou, XQ] H;Hunan Univ Chinese Med, 300 Xueshi Rd,Sci Educ Ind Pk, Changsha 410208, Hunan, Peoples R China.

关键词： Culprit artery;Diagnostic meta-analysis;Electrocardiography;Inferior wall myocardial infarction

摘要： Background: Prior studies have proposed several electrocardiogram (ECG) criteria in limb leads for identifying the culprit coronary artery (CCA) in patients with acute inferior wall myocardial infarction (IWMI). The aim of our study was to conduct an evidence-based evaluation and test accuracy comparison of these criteria. Methods: We searched the PubMed, Embase, and Ovid. Eligible studies to assess the diagnostic performance of ECG criteria predicting CCA in IWMI were reviewed for inclusion. A diagnostic meta-analysis of bivariate approach was performed for pooled estimates of sensitivity and specificity, and meta-regression was implemented to investigate sources of heterogeneity. Results: Twenty-four studies with 4431 unique participants met the inclusion criteria. The pooled sensitivity and specificity for STsegment elevation (STE) in III>II, ST-segment depression (STD) in I, STD in aVL, STD in aVL>I, STE in III>II, and STD in aVL>I were 0.91 (0.88-0.94) and 0.69 (0.53-0.81), 0.80 (0.73-0.87) and 0.69 (0.62-0.76), 0.90 (0.81-0.95) and 0.41 (0.22-0.62), 0.84 (0.750.91) and 0.72 (0.48-0.88), and 0.79 (0.62-0.90) and 1.00 (0.37-1.00), respectively. Heterogeneity investigation showed that whether multi-vessel diseased patients were excluded, sample size, publication year, etc., could influence the diagnostic performance. Conclusion: STE in III > II performed better than other criteria for predicting RCA as CCA in IWMI, and STE in III > II and STD in aVL > I were potential and simple algorithms. ECG could be an effective tool to identify the CCA, but future studies are clearly needed to address the potential of diagnostic and prognostic value. Abbreviations: CAD = coronary artery disease, CAG = coronary angiography, CCA = culprit coronary artery, 95% confidence interval = 95% CI, ECG = electrocardiogram, IVUS = intravascular ultrasound, IWMI = inferior wall myocardial infarction, LAD = left anterior descending artery, LCX = left circumflex coronary artery, MI = myocardial infarction, PCI = percutaneous coronary intervention, RCA = right coronary artery, sROC = summary receiver operating characteristic, STD = ST-segment depression, STE = ST-segment elevation.

语种： 英文

作者： Zheng, H.;Xu, J.;Sun, X.;Zeng, F.;Li, Y.;...

期刊： NEUROGASTROENTEROLOGY AND MOTILITY,2018年30(7):e13316- ISSN：1350-1925

通讯作者： Liang, F. -R.

作者机构： [Liang, F. -R.; Li, Y.; Wu, X.; Zeng, F.; Zheng, H.; Li, J.; Xu, J.; Zhao, L.] Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Coll, Teaching Hosp 3, Chengdu, Sichuan, Peoples R China.;[Sun, X.] Sichuan Univ, West China Hosp, Chinese Evidence Based Med Ctr, Chengdu, Sichuan, Peoples R China.;[Liu, M.; Chang, X. -R.] Hunan Univ Tradit Chinese Med, Acupuncture Coll, Changsha, Hunan, Peoples R China.;[Li, X. -Z.; Gong, B.] Chongqing Med Univ, Tradit Chinese Med Coll, Chongqing, Peoples R China.;[Liang, F. -R.] Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Chengdu, Sichuan, Peoples R China.

通讯机构： [Liang, F. -R.] C;Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Chengdu, Sichuan, Peoples R China.

关键词： electroacupuncture;functional dyspepsia;randomized controlled trial;refractory

摘要： Background: To test the efficacy of electroacupuncture for patients with refractory functional dyspepsia (FD). Methods: A 24-week, 2-arm, single-blind, randomized controlled trial was conducted at three hospitals in China. Patients with refractory FD were randomly assigned to receive 20 sessions of authentic or sham electroacupuncture in a treatment duration of 4 weeks. The primary outcome was complete absence of dyspeptic symptoms at 16 weeks after initiation of acupuncture (week 16). The secondary outcomes included adequate relief of dyspeptic symptoms, Leeds Dyspepsia Questionnaire (LDQ), Nepean Dyspepsia Index (NDI), and adverse events. Intention-to-treat analysis was performed. Key Results: Two hundred patients were included, of which 196 (98%) completed follow-up data at week 24. At week 16, 17 (17%) patients in the authentic electroacupuncture group vs 6 (6%) patients in the sham group achieved the primary outcome (P =.014). Sixty-two (62%) patients had adequate relief in the authentic electroacupuncture group, as compared to 22 (22%) in the sham group (P =.001). The scores of LDQ and NDI were significantly improved in both groups at week 16, and patients in the authentic electroacupuncture group have more improvements (LDQ, mean difference, −2.2, 95% confidence interval, −2.3 to −2.1, P <.001; NDI, −7.3, −10.5 to −4.2, P <.001). Results were similar for all the outcomes assessed at week 24. No serious adverse events were reported in both groups. Conclusion: Acupuncture efficaciously improves dyspeptic symptoms in patients with refractory FD. © 2018 John Wiley & Sons Ltd

语种： 英文

作者： Huang, Xiangjun;Yuan, Lamei;Xu, Hongbo;Zheng, Wen;Cao, Yanna;...

期刊： Bioscience Reports,2018年38(2):BSR20171300. ISSN：0144-8463

通讯作者： Deng, Hao

作者机构： [Huang, Xiangjun] Hunan Univ Chinese Med, Affiliated Hosp 1, Dept Gen Surg, Changsha, Hunan, Peoples R China.;[Li, Yu; Yuan, Lamei; Xu, Hongbo; Deng, Hao; Yang, Zhijian] Cent S Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Hunan, Peoples R China.;[Deng, Hao; Zheng, Wen] Cent S Univ, Xiangya Hosp 3, Dept Neurol, Changsha, Hunan, Peoples R China.;[Yi, Junhui; Cao, Yanna] Cent S Univ, Xiangya Hosp 3, Dept Ophthalmol, Changsha, Hunan, Peoples R China.;[Guo, Yi] Cent S Univ, Informat Secur & Big Data Res Inst, Dept Med Informat, Changsha, Hunan, Peoples R China.

通讯机构： [Deng, Hao] C;Cent S Univ, Xiangya Hosp 3, Ctr Expt Med, Changsha, Hunan, Peoples R China.;Cent S Univ, Xiangya Hosp 3, Dept Neurol, Changsha, Hunan, Peoples R China.

关键词： ABCA4;exome sequencing;inherited retinal degeneration;mutation;retinitis pigmentosa

摘要： Retinitis pigmentosa (RP) is a group of hereditary, degenerative retinal disorders characterized by progressive retinal dysfunction, outer retina cell loss, and retinal tissue atrophy. It eventually leads to tunnel vision and legal or total blindness. Here, we aimed to reveal the causal gene and mutation contributing to the development of autosomal recessive RP (arRP) in a consanguineous family. A novel homozygous mutation, c.4845delT (p.K1616Rfs∗46), in the ATP-binding cassette subfamily A member 4 gene (ABCA4) was identified. It may reduce ABCA4 protein activity, leading to progressive degeneration of both rod and cone photoreceptors. The study extends the arRP genotypic spectrum and confirms a genotype-phenotype relationship. The present study may also disclose some new clues for RP genetic causes and pathogenesis, as well as clinical and genetic diagnosis. The research findingsmay contribute to improvement in clinical care, therapy, genetic screening, and counseling. © 2018 The Author(s).

语种： 英文

作者： Zheng, H.;Liu, Z. -S.;Zhang, W.;Chen, M.;Zhong, F.;...

期刊： NEUROGASTROENTEROLOGY AND MOTILITY,2018年30(7):e13307- ISSN：1350-1925

通讯作者： Li, Y.;Zhu, B.

作者机构： [Li, Y.; Zheng, H.] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Li, Y.; Zhu, W. -Z.; Liu, Z. -S.] China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China.;[Zhong, F.; Zhang, W.] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Chen, M.] Chengdu Univ Tradit Chinese Med, Teaching Hosp, Chengdu, Sichuan, Peoples R China.;[Rong, P. -J.; Zhu, B.; Jing, X. -H.] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.

通讯机构： [Li, Y.; Zhu, B.] C;Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.

关键词： acupuncture;functional constipation;randomized controlled trial

摘要： Background: Acupuncture is used to treat chronic functional constipation (CFC) in China, despite limited evidence. We aim to assess the effectiveness and safety of acupuncture in managing CFC. Methods: A multicenter randomized controlled trial was performed involving 684 patients with CFC; the patients were randomly allocated to receive He acupuncture (n = 172), Shu-mu acupuncture (n = 171), He-shu-mu acupuncture (n = 171), or oral administration of mosapride (n = 170). Sixteen sessions of acupuncture were given in the treatment duration of 4 weeks. The primary outcome was the change in spontaneous bowel movements (SBMs) at week 4 (at the end of treatment) compared to baseline. The secondary outcomes included stool consistency (Bristol scale), the degree of straining during defecation, and adverse events. Key Results: The SBMs increased in all the four groups at week 4, and the magnitude of increase was equivalent in the four groups (He acupuncture, 2.7 [95% CI, 2.3-3.1]; Shu-mu acupuncture, 2.7 [95% CI, 2.3-3.0]; He-shu-mu acupuncture, 2.2 [95% CI, 1.9-2.5]; and mosapride, 2.4 [95% CI, 2.0-2.9]; P =.226). However, the change in SBMs at week 8 was significantly smaller in mosapride group (1.4 [95% CI, 1.0-1.8]) than the three acupuncture groups (2.4 [95% CI, 2.1-2.7], 2.3 [95% CI, 1.9-2.7], 2.1 [95% CI, 1.7-2.5] in He, Shu-mu, and He-shu-mu group, respectively, P =.005). Conclusions & Interferences: The three acupuncture treatments were as effective as mosapride in improving stool frequency and stool consistency in CFC, but the magnitude of the treatment effect is unknown due to the lack of sham acupuncture control. © 2018 John Wiley & Sons Ltd

语种： 英文

作者： Li, Yingchen;Cheng, Qilai;Hu, Guoheng;Deng, Tianhao;Wang, Qimei;...

期刊： EXPERIMENTAL AND THERAPEUTIC MEDICINE,2018年15(5):4067-4079 ISSN：1792-0981

通讯作者： Zhou, Jianda;Su, Xinping

作者机构： [Li, Yingchen] Cent S Univ, Xiangya Hosp 3, Postdoctoral Res Stn Clin Med, Changsha 410013, Hunan, Peoples R China.;[Cheng, Qilai] Gannan Med Univ, Coll Pharm, Ganzhou 341000, Jiangxi, Peoples R China.;[Hu, Guoheng] Hunan Univ Tradit Chinese Med, Dept Neurol, Affiliated Hosp 1, Changsha 410007, Hunan, Peoples R China.;[Wang, Qimei; Deng, Tianhao] Hunan Inst Tradit Chinese Med, Dept Oncol, Affiliated Hosp, Changsha 410006, Hunan, Peoples R China.;[Zhou, Jianda] Cent S Univ, Xiangya Hosp 3, Dept Plast Surg, 138 Tongzhipo Rd, Changsha 410013, Hunan, Peoples R China.

通讯机构： [Zhou, Jianda] C;[Su, Xinping] H;Cent S Univ, Xiangya Hosp 3, Dept Plast Surg, 138 Tongzhipo Rd, Changsha 410013, Hunan, Peoples R China.;Hunan Inst Tradit Chinese Med, Dept Osteol, Affiliated Hosp, 58 Lushan Rd, Changsha 410006, Hunan, Peoples R China.

关键词： exosomes;extracellular vesicles;mesenchymal stromal cells;microvesicles;stroke

摘要： A stroke is a focal cerebral insult that frequently causes severe neurological deficit and mortality. Recent studies have demonstrated that multipotent mesenchymal stromal cells (MSCs) hold great promise for neurovascular remodeling and neurological function recovery following a stroke. Rather than a direct replacement of parenchymal brain cells, the therapeutic mechanism of MSCs is suggested to be the secretion of soluble factors. Specifically, emerging data described MSCs as being able to release extracellular vesicles (EVs), which contain a variety of cargo including proteins, lipids, DNA and various RNA species. The released EVs can target neurocytes and vascular cells and modify the cell's functions by delivering the cargo, which are considered to mediate the neural restoration effects of MSCs. Therefore, EVs may be developed as a novel cell-free therapy for neurological disorders. In the present review, the current advances regarding the components, functions and therapeutic potential of EVs are summarized and the use of MSC-derived EVs as a promising approach in the treatment of stroke are highlighted. © 2018, Spandidos Publications. All rights reserved.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Zou, Guoying;Xu, Fei;Huang, Yiran;Xu, Guofeng;...

期刊： Oncology Letters,2017年14(6):7608-7614 ISSN：1792-1074

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; He, Junyu; Zou, Guoying; Xu, Fei] Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Huang, Yiran; Xu, Guofeng; Xu, Fei] Hunan Univ Chinese Med, Clin Med Sch, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Xu, Guofeng; Yu, Ping] Cent S Univ, Dept Immunol, Sch Basic Med, Changsha 410078, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.

关键词： autoantibody;biomarker;diagnosis;hepatocellular carcinoma;sperm-associated antigen 9

摘要： At present, there is a high incidence of viral hepatitis and high mortality rates due to hepatocellular carcinoma (HCC) in China. In the current study, the quantification of antibodies against the cancer-testis antigen sperm-associated antigen 9 (SPAG9), alone and combined with a-fetoprotein (AFP), were evaluated as biomarkers for the diagnosis of HCC. The levels of anti-SPAG9 antibody and AFP were quantified in serum samples from patients with HCC and hepatitis or cirrhosis, as well as healthy volunteers. The results revealed that the serum levels of anti-SPAG9 immunoglobulin G antibody in patients with HCC were significantly higher compared with those in patients with hepatitis/cirrhosis and healthy controls. Using receiver operator characteristic curves, the area under the curve (AUC, 0.870) of SPAG9 as a diagnostic marker of HCC was significant [P<0.001; 95% confidence interval (CI), 0.793-0.947], whereas the AUC of AFP was 0.832 (P<0.001; 95% CI, 0.736-0.928). Serum anti-SPAG9 antibody levels exhibited significant potential for the differential diagnosis of HCC, with an AUC value of 0.729, (P=0.008; 95% CI, 0.559-0.899). Similarly, serum AFP levels exhibited significant value for the differential diagnosis of HCC, with an AUC value of 0.842 (P<0.001; 95% CI, 0.732-0.953). When combined with quantification of AFP, the diagnostic sensitivity and specificity of anti-SPAG9 levels were increased. In summary, the results suggested that anti-SPAG9 antibody is a potential early diagnostic marker of HCC. © 2017, Spandidos Publications. All rights reserved.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Luo, Shudi;Xu, Fei;Zou, Guoying;Xu, Guofeng;...

期刊： CELL STRESS & CHAPERONES,2017年22(2):237-244 ISSN：1355-8145

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Xu, Guofeng; Xu, Fei] Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zou, Guoying; Huang, Yiran; Xu, Guofeng; Luo, Shudi; Xu, Fei] Hunan Univ Tradit Chinese Med, Sch Clin Med, Changsha 410007, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.

关键词： Heat shock protein 70;Sperm-associated antigen 9;Auto-antibody;Hepatocellular carcinoma;Lung cancer

摘要： There are different views of how the immune system participates in the reaction to cancer. Here, we evaluated expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in patients with hepatocellular carcinoma (HCC) and lung cancer to explore tumor immunity. Our analysis showed that levels of HSP70 and SPAG9 antibody were significantly higher in the serum of lung cancer and HCC patients than in the serum of healthy subjects (P < 0.001), but there were no differences in levels of HSP70 antibody in patients and controls. Levels of serum SPAG9 antibody in newly diagnosed lung cancer patients were significantly higher than in treated lung cancer patients (P < 0.05), but there were no differences in levels of HSP70 or HSP70 antibody. Levels of serum HSP70 and SPAG9 antibody, but not HSP70 antibody, were also higher in hepatitis/cirrhosis patients than in healthy subjects (P = 0.005, P < 0.001). Levels of serum SPAG9 antibody were significantly higher in HCC patients than in hepatitis/cirrhosis patients, but there were no differences in HSP70 or HSP70 antibody levels. Finally, levels of serum HSP70 and SPAG9 antibody were significantly higher in HCC patients than in lung cancer patients (P < 0.05, P < 0.001). These results indicate that cancer-testis antigen SPAG9 induces a strong humoral immune response in cancer patients but HSP70 does not. These results show that SPAG9 has potential as a tumor-specific biomarker.

语种： 英文

作者： Zhao, Ling;Chen, Jiao;Li, Ying;Sun, Xin;Chang, Xiaorong;...

期刊： JAMA INTERNAL MEDICINE,2017年177(4):508-515 ISSN：2168-6106

通讯作者： Liang, Fanrong

作者机构： [Yang, Mingxiao; Li, Ying; Liang, Fanrong; Zhao, Ling; Zheng, Hui; Wu, Xi; Chen, Jiao] Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, 37 Shier Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.;[Sun, Xin] Sichuan Univ, Chinese Cochrane Ctr, West China Hosp, Chengdu, Sichuan, Peoples R China.;[Chang, Xiaorong] Hunan Univ Tradit Chinese Med, Acupuncture & Tuina Sch, Changsha, Hunan, Peoples R China.;[Li, Xuezhi; Gong, Biao] Chongqing Med Univ, Dept Tradit Chinese Med, Chongqing, Peoples R China.;[Huang, Yinlan] Ningxia Med Univ, Dept Tradit Chinese Med, Ningxia, Yinchuan, Peoples R China.

通讯机构： [Liang, Fanrong] C;Chengdu Univ Tradit Chinese Med, Acupuncture & Tuina Sch, 37 Shier Qiao Rd, Chengdu 610075, Sichuan, Peoples R China.

关键词： acupuncture therapy discipline;migraine disorders;migraine prophylaxis;acupuncture procedure;common migraine;waiting lists

摘要： IMPORTANCE The long-term prophylactic effects of acupuncture for migraine are uncertain. OBJECTIVE To investigate the long-term effects of true acupuncture compared with sham acupuncture and being placed in a waiting-list control group for migraine prophylaxis. DESIGN, SETTING, AND PARTICIPANTS Thiswas a 24-week randomized clinical trial (4weeks of treatment followed by 20 weeks of follow-up). Participants were randomly assigned to true acupuncture, sham acupuncture, or a waiting-list control group. The trial was conducted from October 2012 to September 2014 in outpatient settings at 3 clinical sites in China. A total of 249 participants 18 to 65 years old with migraine without aura based on the criteria of the International Headache Society, with migraine occurring 2 to 8 times per month. INTERVENTIONS Participants in the true acupuncture and sham acupuncture groups received treatment 5 days per week for 4 weeks for a total of 20 sessions. Participants in the waiting-list group did not receive acupuncture but were informed that 20 sessions of acupuncture would be provided free of charge at the end of the trial. MAIN OUTCOMES AND MEASURES Participants used diaries to record migraine attacks. The primary outcome was the change in the frequency of migraine attacks from baseline to week 16. Secondary outcome measures included the migraine days, average headache severity, and medication intake every 4 weeks within 24 weeks. RESULTS A total of 249 participants 18 to 65 years old were enrolled, and 245 were included in the intention-to-treat analyses. One hundred eighty-nine (77.1%) were women. Baseline characteristics were comparable across the 3 groups. The mean (SD) change in frequency of migraine attacks differed significantly among the 3 groups at 16 weeks after randomization (P < .001); the mean (SD) frequency of attacks decreased in the true acupuncture group by 3.2 (2.1), in the sham acupuncture group by 2.1 (2.5), and the waiting-list group by 1.4 (2.5); a greater reduction was observed in the true acupuncture than in the sham acupuncture group (difference of 1.1 attacks; 95%CI, 0.4-1.9; P = .002) and in the true acupuncture vs waiting-list group (difference of 1.8 attacks; 95%CI, 1.1-2.5; P < .001). Sham acupuncture was not statistically different from the waiting-list group (difference of 0.7 attacks; 95%CI, -0.1 to 1.4; P = .07). CONCLUSIONS AND RELEVANCE Among patients with migraine without aura, true acupuncture may be associated with long-term reduction in migraine recurrence compared with sham acupuncture or assigned to a waiting list. © 2017 American Medical Association.

语种： 英文

作者： Jin, Liang;Li, Chengyin;Li, Yanping*;Wu, Bin*

期刊： Evidence-Based Complementary and Alternative Medicine,2017年2017:3242301 ISSN：1741-427X

通讯作者： Li, Yanping;Wu, Bin

作者机构： [Jin, Liang; Li, Chengyin; Wu, Bin; Li, Yanping; Wu, B] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;[Jin, Liang] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha 410007, Hunan, Peoples R China.;[Wu, Bin; Li, Yanping; Wu, B] Chongqing Engn Technol Res Ctr Tradit Chinese Med, Chongqing 400021, Peoples R China.

通讯机构： [Li, YP; Wu, B] C;Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;Chongqing Engn Technol Res Ctr Tradit Chinese Med, Chongqing 400021, Peoples R China.

摘要： Objective. To evaluate the clinical efficacy and safety of total glucosides of paeony (TGP) for primary Sjögren's syndrome (pSS). Methods. Eight electronic databases were searched from their inception to July 2016. Clinical randomized controlled trials (RCTs) were included. The study quality was evaluated according to the standard suggested in the Cochrane Handbook. RevMan 5.1 was used for statistical analysis. Results. Seven RCTs involving 443 patients were included. The results showed that TGP combined with an immunosuppressant (IS) showed greater efficacy for improving the saliva flow test of pSS compared to immunosuppressant alone (WMD -6.88, 95% CI -9.02 to -4.74, and P<0.00001). And the same trend favouring TGP-IS dual combination was found in Schirmer test (WMD 1.63, 95% CI 0.26 to 3.01, and P=0.02), ESR (WMD 7.33, 95% CI -10.08 to -4.59, and P<0.00001), CRP (WMD -6.00, 95% CI -7.17 to -4.83, and P<0.00001), IgM (WMD = -0.42, 95% CI -0.70 to 0.13, and P=0.004), and IgG (WMD -3.22, 95% CI -4.32 to -2.12, and P<0.00001) analysis. However, TGP did not affect IgA (WMD 0.53, 95% CI -1.34 to -0.29, and P=0.20). The adverse events manifested no significant differences between the two groups. Conclusions. The TGP-IS combination is superior to IS alone in the treatment of pSS. However, due to the low quality of included studies, high-quality RCTs are needed to confirm the beneficial effects of TGP. © 2017 Liang Jin et al.

语种： 英文

作者： Lu, Cong;Shi, Zhe;Sun, Xiuping;Pan, Ruile;Chen, Shanguang;...

期刊： Journal of Ethnopharmacology,2017年201:73-81 ISSN：0378-8741

通讯作者： Liu, Xinmin

作者机构： [Pan, Ruile; Lu, Cong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;[Pan, Ruile; Lu, Cong; Sun, Xiuping; Shi, Zhe; Liu, Xinmin] Peking Union Med Coll, Beijing, Peoples R China.;[Chen, Shanguang; Li, Yinghui; Qu, Lina] China Astronaut Res & Training Ctr, Beijing, Peoples R China.;[Sun, Lihua; Dang, Haixia] Chinese Acad Chinese Med Sci, Beijing, Peoples R China.;[Chen, Lingling; Bu, Lanlan; Liu, Xinmin] Hunan Univ Tradit Chinese Med, Changsha, Hunan, Peoples R China.

通讯机构： [Liu, Xinmin] C;[Liu, Xinmin] P;[Liu, Xinmin] H;Chinese Acad Med Sci, Inst Med Plant Dev IMPLAD, Res Ctr Pharmacol & Toxicol, Beijing, Peoples R China.;Peking Union Med Coll, Beijing, Peoples R China.

关键词： Abeta(1-40);Cognitive deficits;Kai Xin San;Memory-related molecules;Reward-directed instrumental learning

摘要： Ethnopharmacological relevance Kai Xin San (KXS), a traditional formula of Chinese medicine, has been used to treat dementia. Aim of the study The present study aimed to investigate its ameliorating effects on Aβ 1-40 -induced cognitive impairment in rats using a series of novel reward-directed instrumental learning tasks, and to determine its possible mechanism of action. Materials and methods Rats were pretreated with KXS aqueous extract (0.72 and 1.44 g/kg, p.o.) for 10 days, and were trained to gain reward reinforcement by lever pressing at the meantime. Thereafter, rats received a bilateral microinjection of Aβ 1-40 in CA1 regions of the hippocampus. Cognitive performance was evaluated with the goal directed (higher response ratio) and habit (visual signal discrimination and extinction) learning tasks, as well as on the levels of memory-related biochemical parameters and molecules. Results Our findings first demonstrated that KXS can improve Aβ 1-40 -induced amnesia in RDIL via enhancing the comprehension of action-outcome association and the utilization of cue information to guide behavior. Then, its ameliorating effects should be attributed to the modulation of memory-related molecules in the hippocampus. Conclusion In conclusion, KXS has the potential to prevent and/or delay the deterioration of cognitive impairment in AD. © 2017 Elsevier Ireland Ltd

语种： 英文