作者机构：
[Tian, Xiao-Ting; Ba, Hui-Xue; Cao, Yuan-Yuan; Li, Xiao-Hui] Cent South Univ, Xiangya Sch Pharmaceut Sci, Dept Pharmacol, Changsha 410013, Peoples R China.;[Li, Ke] Hunan Acad Tradit Chinese Med, Changsha 410013, Hunan, Peoples R China.;[Li, Ying] Cent South Univ, Xiangya Hosp 3, Dept Hlth Management, Changsha, Peoples R China.;[Li, Xiao-Hui] Hunan Key Lab Bioanal Complex Matrix Samples, Changsha, Peoples R China.;[Wang, Aiping] Univ South China, Nanhua Affiliated Hosp, Inst Clin Res, Hengyang, Peoples R China.
通讯机构：
[Ke Li] H;[Xiao-Hui Li] D;Hunan Academy of Traditional Chinese Medicine, Hunan, China<&wdkj&>Department of Pharmacology, Xiangya School of Pharmaceutical Science, Central South University, Changsha, China<&wdkj&>Hunan Key Laboratory for Bioanalysis of Complex Matrix Samples, Changsha, China
期刊：
AMERICAN JOURNAL OF CHINESE MEDICINE,2020年48(3):559-577 ISSN：0192-415X
通讯作者：
Wang, Yong-Jun;Zhang, Y;Yuan, TF
作者机构：
[Wang, Yong-Jun; Sha, Nan-Nan; Shu, Bing; He, Ming-Chao; Sun, Yue-Li; Li, Yue; Zhang, Yan; Wang, YJ] Shanghai Univ Tradit Chinese Med, Longhua Hosp, Spine Dis Res Inst, Room 908,Bldg 12,725 South Wanping Rd, Shanghai 200032, Peoples R China.;[Wang, Yong-Jun; Shu, Bing; He, Ming-Chao; Sun, Yue-Li; Li, Yue; Zhang, Yan] Minist Educ, Key Lab Theory & Therapy Muscles & Bones, Shanghai 200032, Peoples R China.;[Yuan, Ti-Fei; Shi, Zhe] Shanghai Jiao Tong Univ, Shanghai Mental Hlth Ctr, Shanghai Key Lab Psychot Disorders, Sch Med, Shanghai 201108, Peoples R China.;[Liao, Duan-Fang; Shi, Zhe] Hunan Univ Chinese Med, Div Stem Cell Regulat & Applicat, Key Lab Qual Evaluat Bulk Herbs Hunan Prov, Changsha 410208, Peoples R China.;[Qin, Meng] Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China.
通讯机构：
[Yuan, TF ; Zhang, Y ; Wang, YJ] S;Shanghai Univ Tradit Chinese Med, Longhua Hosp, Spine Dis Res Inst, Room 908,Bldg 12,725 South Wanping Rd, Shanghai 200032, Peoples R China.;Shanghai Jiao Tong Univ, Shanghai Mental Hlth Ctr, Shanghai Key Lab Psychot Disorders, Sch Med, Shanghai 201108, Peoples R China.
关键词：
Depression;Muscone;Microglia;Neuroinflammation;Renin-Angiotensin System
摘要：
<jats:p> Depression is partially caused by inflammation in the central nervous system. Early study demonstrated that musk, glandular secretion from male musk deer, exerted an antidepressant-like effect. The aim of this study was to investigate if muscone, a bioactive ingredient in musk, could ameliorate neuroinflammation and depressive-like behaviors as well as explore the potential action mechanism. Mice were intraperitoneally (i.p.) injected with muscone for 2 weeks prior to administration of lipopolysaccharides (LPS, 1[Formula: see text]mg/kg, i.p.). Pre-treatment with muscone reversed the LPS-induced decrease in body weight within 24[Formula: see text]h and ameliorated depressive-like behaviors shown by sucrose preference, tail suspension test, and forced swimming test. LPS-induced activation of microglial cells and elevation in expression of inflammatory cytokines including IL-1[Formula: see text], RANTES, and MCP-1 in the prefrontal cortex of mice were effectively abrogated by muscone, which significantly down-regulated expression of TLR4, MyD88, Caspase-1, NLRP3, renin, and Ang II. In addition, treatment of BV2 microglia cells with muscone markedly attenuated the LPS-induced rise in protein expression of TLR4, Ang II, and IL-1[Formula: see text]. This study revealed that muscone could ameliorate LPS-induced depressive-like behaviors by repressing neuroinflammation in the prefrontal cortex of mice caused by its suppression on microglia activation and production of inflammatory cytokines via acting on TLR4 pathway and RAS cascade. </jats:p>
摘要：
ImportanceThe effects of acupuncture as adjunctive treatment to antianginal therapies for patients with chronic stable angina are uncertain.ObjectiveTo investigate the efficacy and safety of acupuncture as adjunctive therapy to antianginal therapies in reducing frequency of angina attacks in patients with chronic stable angina.Design, Setting, and ParticipantsIn this 20-week randomized clinical trial conducted in outpatient and inpatient settings at 5 clinical centers in China from October 10, 2012, to September 19, 2015, 404 participants were randomly assigned to receive acupuncture on the acupoints on the disease-affected meridian (DAM), receive acupuncture on the acupoints on the nonaffected meridian (NAM), receive sham acupuncture (SA), and receive no acupuncture (wait list [WL] group). Participants were 35 to 80 years of age with chronic stable angina based on the criteria of the American College of Cardiology and the American Heart Association, with angina occurring at least twice weekly. Statistical analysis was conducted from December 1, 2015, to July 30, 2016.InterventionsAll participants in the 4 groups received antianginal therapies as recommended by the guidelines. Participants in the DAM, NAM, and SA groups received acupuncture treatment 3 times weekly for 4 weeks for a total of 12 sessions. Participants in the WL group did not receive acupuncture during the 16-week study period.Main Outcomes and MeasuresParticipants used diaries to record angina attacks. The primary outcome was the change in frequency of angina attacks every 4 weeks from baseline to week 16.ResultsA total of 398 participants (253 women and 145 men; mean [SD] age, 62.6 [9.7] years) were included in the intention-to-treat analyses. Baseline characteristics were comparable across the 4 groups. Mean changes in frequency of angina attacks differed significantly among the 4 groups at 16 weeks: a greater reduction of angina attacks was observed in the DAM group vs the NAM group (difference, 4.07; 95% CI, 2.43-5.71;<i>P</i> < .001), in the DAM group vs the SA group (difference, 5.18; 95% CI, 3.54-6.81;<i>P</i> < .001), and in the DAM group vs the WL group (difference, 5.63 attacks; 95% CI, 3.99-7.27;<i>P</i> < .001).Conclusions and RelevanceCompared with acupuncture on the NAM, SA, or no acupuncture (WL), acupuncture on the DAM as adjunctive treatment to antianginal therapy showed superior benefits in alleviating angina.Trial RegistrationClinicalTrials.gov identifier:NCT01686230
摘要：
Lonicera macranthoides Hand. -Mazz. is an important medicinal and economical plant in China, however, the codon usage bias (CUB) in L. macranthoides genes is still unknown. In this study, L. macranthoides transcriptome sequencing has been completed, and codon usage patterns in 36,090 reconstructed genes from the L. macranthoides transcriptome were examined. The mean GC content and GC3 value is 44.9% and 43.1%, respectively, which indicates that nucleotide contents of L. macranthoides genome is somewhat AT rich, and its codon bias pattern tends to use A/T-ending codons. According to neutrality plot, ENC plot, PR2-Bias plot and correspondence analysis, we know that both compositional constraint under selection and mutation could affect the CUB in L. macranthoides, and the mutation is the most determinant factor. Meanwhile, gene expression levels can influence its codon usage pattern. Furthermore, we identified 29 optimal codons and most of them ended with A/U. The study will lay a foundation for future research on gene prediction, genetic engineering and molecular evolution in L. macranthoides.
摘要：
Acupuncture may be beneficial for patients with mild hypertension, but the evidence is not convincing. We aimed to examine the effect of acupuncture on blood pressure (BP) reduction in patients with mild hypertension. We conducted a multicenter, single-blind, sham-controlled, randomized trial in eleven hospitals in China. The trial included 428 patients with systolic blood pressure (SBP) from 140 to 159 mm Hg and/or with diastolic blood pressure (DBP) from 90 to 99 mm Hg. The patients were randomly assigned to receive 18 sessions of affected meridian acupuncture (n = 107) or non-affected meridian acupuncture (n = 107) or sham acupuncture (n = 107) during 6 weeks, or to stay in a waiting-list control (n = 107). All patients received 24-hour ambulatory blood pressure monitoring at weeks 6, 9, and 12. We included 415 participants in the intention-to-treat analysis. The two acupuncture groups were pooled in the analysis, since they had no difference in all outcomes. SBP decreased at week 6 in acupuncture group vs sham acupuncture vs waiting-list group (7.2 +/- 11.0 mm Hg vs 4.1 +/- 11.5 mm Hg vs 4.1 +/- 13.2 mm Hg); acupuncture was not superior to sham acupuncture (mean difference 2.7 mm Hg, 95% CI 0.4 to 5.9, adjusted P = 0.103) or waiting-list control (2.9 mm Hg, 95% CI -0.2 to 6.0, adjusted P = 0.078). However, acupuncture was superior to sham acupuncture (3.3 mm Hg, 95% CI 0.2 to 6.3, adjusted P = 0.035) and waiting-list control (4.8 mm Hg, 95% CI 1.8 to 7.8, P < 0.001) at week 9. Acupuncture had a small effect size on the reduction of BP in patients with mild hypertension.
摘要：
Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVADs). The infection of PCV2 is widespread and has serious consequence, thereby causing significant economic losses in the swine industry worldwide. Previously, we found that a strain named YiY-3-2-3 has a naturally occurring point mutation (G710 to A710) in ORF1 region, which leads to a shorten product of the rep gene (945 to 660 base pair). Importantly, the Rep protein is responsible for genome replication of PCV2. To explore the effects of this mutation on the PCV2 replication, in the current study we constructed infectious clone of this IF-YiY-3-2-3, as well as those of its two parental strains of IF-YiY-3-2-1 and IF-YiY-3-2-10. Subsequently, these infectious clones which have 1.1 copy of PCV2 genome of their corresponding strains were transfected into PK15 cells to obtain rescued viruses, respectively. Though all of the three infectious clones could be rescued, the copy number and infectivity of these rescued viruses were significantly different, as analyzed by fluorescence quantitative PCR, Tissue culture infectious dose 50 (TCID50), and indirect immunofluorescence assay (IFA). Notably, whether the PCV2 copy number, viral titer or the infectivity of rescued viruses from infectious clone IF-YiY-3-2-3 was significantly less than those of its parental clones. Meanwhile, the spatial structure of the Rep protein from the IF-YiY-3-2-3 displayed an apparent truncation at the C-terminal. These findings therefore suggest that the Rep protein with truncated C-terminal would reduce virus replication and infectivity, and there might also exist both favorable and unfavorable mutations in the ORF1 of PCV2 in the process of its evolution.
期刊：
MEDICAL SCIENCE MONITOR,2019年25:8152-8171 ISSN：1643-3750
通讯作者：
Li, YH;Zeng, LT
作者机构：
[Bao, Tingting] Beijing Univ Chinese Med, Beijing, Peoples R China.;[Li, YH; Li, Yuehua; Bao, Tingting] China Acad Chinese Mec Sci, Xiyuan Hosp, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing, Peoples R China.;[Yang, Kailin] Capital Med Univ, Beijing Anzhen Hosp, Beijing, Peoples R China.;[Long, Zhiyong] Shantou Univ, Med Coll, Shantou, Guangdong, Peoples R China.
通讯机构：
[Li, YH ] C;[Zeng, LT ] H;China Acad Chinese Mec Sci, Xiyuan Hosp, Beijing, Peoples R China.;Hunan Univ Chinese Med, Changsha, Hunan, Peoples R China.
作者：
Zheng, H.;Liu, Z. -S.;Zhang, W.;Chen, M.;Zhong, F.;...
期刊：
NEUROGASTROENTEROLOGY AND MOTILITY,2018年30(7):e13307- ISSN：1350-1925
通讯作者：
Li, Y;Zhu, B
作者机构：
[Li, Y; Zheng, H.] Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;[Li, Y.; Zhu, W. -Z.; Liu, Z. -S.] China Acad Chinese Med Sci, Guanganmen Hosp, Beijing, Peoples R China.;[Zhong, F.; Zhang, W.] Hunan Univ Tradit Chinese Med, Affiliated Hosp 1, Changsha, Hunan, Peoples R China.;[Chen, M.] Chengdu Univ Tradit Chinese Med, Teaching Hosp, Chengdu, Sichuan, Peoples R China.;[Rong, P. -J.; Zhu, B.; Jing, X. -H.] China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
通讯机构：
[Li, Y ; Zhu, B ] C;Chengdu Univ Tradit Chinese Med, Chengdu, Sichuan, Peoples R China.;China Acad Chinese Med Sci, Inst Acupuncture & Moxibust, Beijing, Peoples R China.
关键词：
Heat shock protein 70;Sperm-associated antigen 9;Auto-antibody;Hepatocellular carcinoma;Lung cancer
摘要：
There are different views of how the immune system participates in the reaction to cancer. Here, we evaluated expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in patients with hepatocellular carcinoma (HCC) and lung cancer to explore tumor immunity. Our analysis showed that levels of HSP70 and SPAG9 antibody were significantly higher in the serum of lung cancer and HCC patients than in the serum of healthy subjects (P < 0.001), but there were no differences in levels of HSP70 antibody in patients and controls. Levels of serum SPAG9 antibody in newly diagnosed lung cancer patients were significantly higher than in treated lung cancer patients (P < 0.05), but there were no differences in levels of HSP70 or HSP70 antibody. Levels of serum HSP70 and SPAG9 antibody, but not HSP70 antibody, were also higher in hepatitis/cirrhosis patients than in healthy subjects (P = 0.005, P < 0.001). Levels of serum SPAG9 antibody were significantly higher in HCC patients than in hepatitis/cirrhosis patients, but there were no differences in HSP70 or HSP70 antibody levels. Finally, levels of serum HSP70 and SPAG9 antibody were significantly higher in HCC patients than in lung cancer patients (P < 0.05, P < 0.001). These results indicate that cancer-testis antigen SPAG9 induces a strong humoral immune response in cancer patients but HSP70 does not. These results show that SPAG9 has potential as a tumor-specific biomarker.
摘要：
ImportanceThe long-term prophylactic effects of acupuncture for migraine are uncertain.ObjectiveTo investigate the long-term effects of true acupuncture compared with sham acupuncture and being placed in a waiting-list control group for migraine prophylaxis.Design, Setting, and ParticipantsThis was a 24-week randomized clinical trial (4 weeks of treatment followed by 20 weeks of follow-up). Participants were randomly assigned to true acupuncture, sham acupuncture, or a waiting-list control group. The trial was conducted from October 2012 to September 2014 in outpatient settings at 3 clinical sites in China. A total of 249 participants 18 to 65 years old with migraine without aura based on the criteria of the International Headache Society, with migraine occurring 2 to 8 times per month.InterventionsParticipants in the true acupuncture and sham acupuncture groups received treatment 5 days per week for 4 weeks for a total of 20 sessions. Participants in the waiting-list group did not receive acupuncture but were informed that 20 sessions of acupuncture would be provided free of charge at the end of the trial.Main Outcomes and MeasuresParticipants used diaries to record migraine attacks. The primary outcome was the change in the frequency of migraine attacks from baseline to week 16. Secondary outcome measures included the migraine days, average headache severity, and medication intake every 4 weeks within 24 weeks.ResultsA total of 249 participants 18 to 65 years old were enrolled, and 245 were included in the intention-to-treat analyses. One hundred eighty-nine (77.1%) were women. Baseline characteristics were comparable across the 3 groups. The mean (SD) change in frequency of migraine attacks differed significantly among the 3 groups at 16 weeks after randomization (<i>P</i> < .001); the mean (SD) frequency of attacks decreased in the true acupuncture group by 3.2 (2.1), in the sham acupuncture group by 2.1 (2.5), and the waiting-list group by 1.4 (2.5); a greater reduction was observed in the true acupuncture than in the sham acupuncture group (difference of 1.1 attacks; 95% CI, 0.4-1.9;<i>P</i> = .002) and in the true acupuncture vs waiting-list group (difference of 1.8 attacks; 95% CI, 1.1-2.5;<i>P</i> < .001). Sham acupuncture was not statistically different from the waiting-list group (difference of 0.7 attacks; 95% CI, −0.1 to 1.4;<i>P</i> = .07).Conclusions and RelevanceAmong patients with migraine without aura, true acupuncture may be associated with long-term reduction in migraine recurrence compared with sham acupuncture or assigned to a waiting list.Trial Registrationclinicaltrials.gov Identifier:NCT01687660
期刊：
Evidence-Based Complementary and Alternative Medicine,2017年2017:1-9 ISSN：1741-427X
通讯作者：
Li, Yanping;Wu, B
作者机构：
[Jin, Liang; Li, Chengyin; Li, Yanping; Wu, Bin; Wu, B] Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;[Jin, Liang] Hunan Univ Tradit Chinese Med, Grad Sch, Changsha 410007, Hunan, Peoples R China.;[Li, Yanping; Wu, Bin; Wu, B] Chongqing Engn Technol Res Ctr Tradit Chinese Med, Chongqing 400021, Peoples R China.
通讯机构：
[Li, YP; Wu, B ] C;Chongqing Hosp Tradit Chinese Med, Dept Rheumatol, Chongqing 400021, Peoples R China.;Chongqing Engn Technol Res Ctr Tradit Chinese Med, Chongqing 400021, Peoples R China.
摘要：
<jats:p><jats:italic>Objective</jats:italic>. To evaluate the clinical efficacy and safety of total glucosides of paeony (TGP) for primary Sjögren’s syndrome (pSS).<jats:italic> Methods</jats:italic>. Eight electronic databases were searched from their inception to July 2016. Clinical randomized controlled trials (RCTs) were included. The study quality was evaluated according to the standard suggested in the Cochrane Handbook. RevMan 5.1 was used for statistical analysis.<jats:italic> Results</jats:italic>. Seven RCTs involving 443 patients were included. The results showed that TGP combined with an immunosuppressant (IS) showed greater efficacy for improving the saliva flow test of pSS compared to immunosuppressant alone (WMD −6.88, 95% CI −9.02 to −4.74, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.00001</mml:mn></mml:math>). And the same trend favouring TGP-IS dual combination was found in Schirmer test (WMD 1.63, 95% CI 0.26 to 3.01, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">0.02</mml:mn></mml:math>), ESR (WMD 7.33, 95% CI −10.08 to −4.59, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0</mml:mn><mml:mo>.</mml:mo><mml:mn fontstyle="italic">00001</mml:mn></mml:math>), CRP (WMD −6.00, 95% CI −7.17 to −4.83, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.00001</mml:mn></mml:math>), IgM (WMD = −0.42, 95% CI −0.70 to 0.13, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">0.004</mml:mn></mml:math>), and IgG (WMD −3.22, 95% CI −4.32 to −2.12, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M6"><mml:mi>P</mml:mi><mml:mo><</mml:mo><mml:mn fontstyle="italic">0.00001</mml:mn></mml:math>) analysis. However, TGP did not affect IgA (WMD 0.53, 95% CI −1.34 to −0.29, and <mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M7"><mml:mi>P</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">0.20</mml:mn></mml:math>). The adverse events manifested no significant differences between the two groups.<jats:italic> Conclusions</jats:italic>. The TGP-IS combination is superior to IS alone in the treatment of pSS. However, due to the low quality of included studies, high-quality RCTs are needed to confirm the beneficial effects of TGP.</jats:p>
摘要：
The β1-adrenergic receptor (AR) is the primary β-AR subtype in the heart and is the target of metoprolol (Met), which is commonly used to treat angina and hypertension. Previous studies have revealed a positive correlation between the methylation levels of the adrenoreceptor β1 gene (Adrb1) promoter in the myocardium with the antihypertensive activity of Met in spontaneously hypertensive rats (SHR), which affects β1-AR expression in H9C2 cells. The aim of the present study was to investigate the effects of myocardial β1-AR downregulation using short-hairpin RNA (shRNA) against Adrb1 on the antihypertensive activity of Met in SHR. Recombinant adeno-associated virus type 9 (rAAV9) vectors carrying Adrb1 shRNA (rAAV9-Adrb1) or a negative control sequence (rAAV9-NC) were generated and used to infect rat hearts via the pericardial cavity. The results of reverse transcription-quantitative polymerase chain reaction, immunohistochemistry and western blotting analyses demonstrated that cardiac β1-AR expression in the rAAV9-Adrb1 group was significantly downregulated when compared with the rAAV9-NC group (P<0.001, P<0.001 and P=0.032, respectively). In addition, a greater reduction in systolic blood pressure (SBP) was observed in the rAAV9-NC group compared with the rAAV9-Adrb1 group following Met treatment (P=0.035). Furthermore, downregulation of myocardial β1-AR was associated with a significant decrease in SBP (P<0.001). In conclusion, these data suggest that suppression of β1-AR expression in the myocardium reduces SBP and sensitivity to Met in SHR.
