作者： Ren, Biqiong*（任碧琼）;Luo, Shudi;Tian, Xuefei（田雪飞）;Jiang, Zhichao;Zou, Guoying;...

期刊： Oncology Reports,2018年40(2):895-901 ISSN：1021-335X

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Tian, Xuefei; Ren, Biqiong; Zou, Guoying; Jiang, Zhichao; Yin, Tieqiu; Xu, Fei] Hunan Univ Chinese Med, Peoples Hosp Hunan Prov 2, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.;[Tian, Xuefei; Ren, Biqiong; Zou, Guoying; Huang, Yiran; Liu, Junlong; Jiang, Zhichao; Yin, Tieqiu; Luo, Shudi; Xu, Fei] Hunan Univ Chinese Med, Clin Med Coll, Dept Clin Lab Med, Changsha 410007, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Univ Chinese Med, Peoples Hosp Hunan Prov 2, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.

关键词： liver cancer;HepG2;eHSP70;TLR4;curcumin

摘要： Curcumin has been revealed to inhibit liver cancer, however, no studies have reported that the mechanism of curcumin's action on liver cancer is related to damage-associated molecular pattern (DAMP) molecules heat shock protein 70 (HSP70) and the toll-like receptor 4 (TLR4) signaling. This study aimed to investigate whether the activation of TLR4 signaling by HSP70 could be inhibited by curcumin, thus investigating the possible mechanism of curcumin in the inhibition of liver cancer. Western blotting was used to evaluate the expression of the HSP70 and TLR4 in HepG2 cells and ELISA was used to detect the concentration of HSP70 in cell culture medium. A thermal tolerance HepG2 (HepG2TT) cell model was established to simulate HSP70 accumulation in the microenvironment. A certain concentration of curcumin was co-cultured with HepG2 and HepG2TT cells to observe the changes of HSP70 and TLR4. Our results revealed that heat stress significantly increased the expression of extracellular HSP70 (eHSP70) and TLR4 (P<0.01), but significantly reduced the expression of intracellular HSP70 (P<0.01). Curcumin inhibited proliferation, invasion, and metastasis of HepG2 cells, caused cells to remain in the DNA S phase, promoted apoptosis, and significantly reduced intracellular HSP70, eHSP70 and TLR4 levels of HepG2TT cells. Following the removal of curcumin, eHSP70 increased again. In summary, our results demonstrated that the antitumor effect of curcumin was related to the inhibition HSP70-TLR4 signaling.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Zou, Guoying;He, Junyu;Huang, Yiran;Ma, Guoan;...

期刊： Oncology Letters,2018年15(1):415-422 ISSN：1792-1074

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; Zou, Guoying; He, Junyu; Xu, Guofeng; Li, Yong] Hunan Prov Second Peoples Hosp, Dept Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; Zou, Guoying; He, Junyu; Huang, Yiran; Xu, Guofeng; Li, Yong] Hunan Univ Chinese Med, Clin Med Sch, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; Zou, Guoying; He, Junyu; Ma, Guoan; Yu, Ping] Cent S Univ, Sch Basic Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China.;[Ma, Guoan] Tumor Hosp Hunan Prov, Dept Hepatobiliary Dis, Changsha 410008, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Dept Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.

关键词： cell cycle;cell migration;cell proliferation;hepatoma carcinoma;short interfering RNA;sperm-associated antigen 9

摘要： The incidence and mortality rates of hepatocellular carcinoma (HCC) are higher in China compared with in other countries. Further research is required in order to improve the diagnosis and treatment of HCC. Sperm-associated antigen 9 (SPAG9) protein has been revealed to serve an important function in cancer progression; however, the underlying mechanisms remain to be elucidated. The present study investigated the expression level of SPAG9 in HCC tissues using quantitative-polymerase chain reaction, immunohistochemistry and western blotting, and the results demonstrated that SPAG9 was overexpressed in HCC tissues compared with the adjacent non-cancerous tissues. To explore the potential mechanisms underlying SPAG9 in HCC, the effect of SPAG9 on cell proliferation, cell cycle, migration and invasion capacities were investigated in the QGY HCC cell line by RNA interference. It was revealed that inhibition of SPAG9 mRNA in QGY cells significantly inhibited the expression level of SPAG9 compared with the control. Depletion of SPAG9 expression decreased cell proliferation (P<0.01) and increased the percentage of cells in the G1/G2 cell cycle phase. The percentage of cells in the S phase was decreased, and cell migration and invasion capabilities in vitro were reduced (P<0.01). In summary, the results of the present study suggested that SPAG9 was upregulated in HCC and may serve an important function in cancer cell proliferation, differentiation and invasion. Whether SPAG9 is a potential diagnostic marker and therapeutic target of human HCC requires additional study. © 2018, Spandidos Publications. All rights reserved.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Zou, Guoying;Xu, Fei;Huang, Yiran;Xu, Guofeng;...

期刊： Oncology Letters,2017年14(6):7608-7614 ISSN：1792-1074

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; He, Junyu; Zou, Guoying; Xu, Fei] Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Huang, Yiran; Xu, Guofeng; Xu, Fei] Hunan Univ Chinese Med, Clin Med Sch, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Xu, Guofeng; Yu, Ping] Cent S Univ, Dept Immunol, Sch Basic Med, Changsha 410078, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd, Changsha 410007, Hunan, Peoples R China.

关键词： autoantibody;biomarker;diagnosis;hepatocellular carcinoma;sperm-associated antigen 9

摘要： At present, there is a high incidence of viral hepatitis and high mortality rates due to hepatocellular carcinoma (HCC) in China. In the current study, the quantification of antibodies against the cancer-testis antigen sperm-associated antigen 9 (SPAG9), alone and combined with a-fetoprotein (AFP), were evaluated as biomarkers for the diagnosis of HCC. The levels of anti-SPAG9 antibody and AFP were quantified in serum samples from patients with HCC and hepatitis or cirrhosis, as well as healthy volunteers. The results revealed that the serum levels of anti-SPAG9 immunoglobulin G antibody in patients with HCC were significantly higher compared with those in patients with hepatitis/cirrhosis and healthy controls. Using receiver operator characteristic curves, the area under the curve (AUC, 0.870) of SPAG9 as a diagnostic marker of HCC was significant [P<0.001; 95% confidence interval (CI), 0.793-0.947], whereas the AUC of AFP was 0.832 (P<0.001; 95% CI, 0.736-0.928). Serum anti-SPAG9 antibody levels exhibited significant potential for the differential diagnosis of HCC, with an AUC value of 0.729, (P=0.008; 95% CI, 0.559-0.899). Similarly, serum AFP levels exhibited significant value for the differential diagnosis of HCC, with an AUC value of 0.842 (P<0.001; 95% CI, 0.732-0.953). When combined with quantification of AFP, the diagnostic sensitivity and specificity of anti-SPAG9 levels were increased. In summary, the results suggested that anti-SPAG9 antibody is a potential early diagnostic marker of HCC. © 2017, Spandidos Publications. All rights reserved.

语种： 英文

作者： Ren, Biqiong*（任碧琼）;Luo, Shudi;Xu, Fei;Zou, Guoying;Xu, Guofeng;...

期刊： CELL STRESS & CHAPERONES,2017年22(2):237-244 ISSN：1355-8145

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; He, Junyu; Zhu, Haowen; Li, Yong; Zou, Guoying; Xu, Guofeng; Xu, Fei] Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zou, Guoying; Huang, Yiran; Xu, Guofeng; Luo, Shudi; Xu, Fei] Hunan Univ Tradit Chinese Med, Sch Clin Med, Changsha 410007, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.

关键词： Heat shock protein 70;Sperm-associated antigen 9;Auto-antibody;Hepatocellular carcinoma;Lung cancer

摘要： There are different views of how the immune system participates in the reaction to cancer. Here, we evaluated expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in patients with hepatocellular carcinoma (HCC) and lung cancer to explore tumor immunity. Our analysis showed that levels of HSP70 and SPAG9 antibody were significantly higher in the serum of lung cancer and HCC patients than in the serum of healthy subjects (P < 0.001), but there were no differences in levels of HSP70 antibody in patients and controls. Levels of serum SPAG9 antibody in newly diagnosed lung cancer patients were significantly higher than in treated lung cancer patients (P < 0.05), but there were no differences in levels of HSP70 or HSP70 antibody. Levels of serum HSP70 and SPAG9 antibody, but not HSP70 antibody, were also higher in hepatitis/cirrhosis patients than in healthy subjects (P = 0.005, P < 0.001). Levels of serum SPAG9 antibody were significantly higher in HCC patients than in hepatitis/cirrhosis patients, but there were no differences in HSP70 or HSP70 antibody levels. Finally, levels of serum HSP70 and SPAG9 antibody were significantly higher in HCC patients than in lung cancer patients (P < 0.05, P < 0.001). These results indicate that cancer-testis antigen SPAG9 induces a strong humoral immune response in cancer patients but HSP70 does not. These results show that SPAG9 has potential as a tumor-specific biomarker.

语种： 英文

作者： 邹国英;伏尹;徐国锋;任碧琼（任碧琼）

期刊： 卒中与神经疾病,2017年24(5):472-474 ISSN：1007-0478

作者机构： 湖南省脑科医院检验科, 长沙,410007;湖南中医药大学临床医学院检验教研室;[任碧琼; 邹国英; 徐国锋] 湖南省脑科医院;[伏尹] 湖南中医药大学临床医学院

关键词： 出血性脑卒中;药物治疗方法;单核巨噬细胞;血管再生;平滑肌细胞;成纤维细胞;不良预后;内皮细胞

摘要： <正>出血性脑卒中（Hemorrhagic stroke,HS）起病急骤、病情凶险,具有较高病死率和不良预后率特征,目前还没有令人满意的药物治疗方法。PTX3（Pentraxin-3）是正五聚蛋白超家族中的一员,内皮细胞、平滑肌细胞、脂肪细胞、成纤维细胞、单核巨噬细胞及树突细胞等均可分泌PTX3,能反映血管的炎症状态,并与神经和血管再生有关,对脑卒中起功能性修复作用,可能成为治疗HS的新靶点。

语种： 中文

作者： Ren, Biqiong*（任碧琼）;Zou, Guoying;Huang, Yiran;Xu, Guofeng;Xu, Fei;...

期刊： Cell Stress & Chaperones,2016年21(4):677-686 ISSN：1355-8145

通讯作者： Ren, Biqiong（任碧琼）

作者机构： [Ren, Biqiong; He, Junyu; Zhu, Haowen; Zou, Guoying; Xu, Guofeng; Xu, Fei] Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zhu, Haowen; Zou, Guoying; Huang, Yiran; Xu, Guofeng; Xu, Fei] Hunan Univ Chinese Med, Sch Clin Med, Changsha 410007, Hunan, Peoples R China.;[Ren, Biqiong; He, Junyu; Zou, Guoying; Yu, Ping] Cent South Univ, Sch Basic Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China.

通讯机构： [Ren, Biqiong] H;[Ren, Biqiong] C;Hunan Prov Second Peoples Hosp, Clin Lab, 427 Furong Rd Changsha, Changsha 410007, Hunan, Peoples R China.;Hunan Univ Chinese Med, Sch Clin Med, Changsha 410007, Hunan, Peoples R China.;Cent South Univ, Sch Basic Med, Dept Immunol, Changsha 410078, Hunan, Peoples R China.

关键词： Biomarkers;DAMPs;Diagnosis;HSP70;Infection;Trauma

摘要： Danger-associated molecular patterns (DAMPs) are activated by endogenous signals that originate from stressed, injured, or necrotic cells, signifying “danger” to the host. In this study, we evaluated the expression of the DAMP heat shock protein 70 (HSP70) in trauma patients with and without secondary infections. Levels of glucose (GLU), procalcitonin (PCT), total cholesterol (T-Chol), and white blood cell (WBC) counts were also evaluated at three time stages after trauma. Our analysis showed that the levels of serum HSP70 in patients with minor, moderate, and severe injuries were significantly higher than in healthy patients at each time point post-injury (P < 0.01), and levels of serum HSP70 in the severe injury group were significantly higher than in the minor injury group at 1–6 h after trauma (P = 0.047). HSP70 was correlated with GLU and was negatively correlated with T-Chol in the period 1–6 h after injury (P = 0.008/0.032). WBC and GLU were elevated after trauma, with mutual positive correlation (P < 0.001). PCT levels increased later than WBC counts and GLU levels; these levels were correlated at the two later time periods, 24–48 h and 60–90 h (P = 0.008/0.041). PCT continued to rise in patients with secondary infection, but PCT dropped at the third time period in patients without secondary infection. In summary, our results suggest that danger and stress theory can be used to predict severity of trauma. © 2016, Cell Stress Society International.

语种： 英文

作者： 邹国英;谭李红;徐飞;朱浩稳;任碧琼（任碧琼）

期刊： 现代检验医学杂志,2016年31(5):150-152,156 ISSN：1671-7414

作者机构： 湖南省脑科医院检验科;湖南中医药大学临床医学院检验教研室;[任碧琼; 朱浩稳] 湖南省第二人民医院;[徐飞] 湖南中医药大学临床医学院;[谭李红; 邹国英] 湖南省脑科医院

关键词： 临床实验室;生物安全;检验医学生;培训

摘要： 目的：评估检验医学生实习前生物安全理论强化训练的效果。方法收集湖南三所大学实习前医学检验专业学生共220名，按不同学校、同学校不同年级、同班级培训前后方法进行分组，按照基本概念、微生物危害评估、实验室个人防护装备、生物安全操作技术规范四个方面内容进行培训，并于培训前后进行无记名现场问卷调查，通过比较评价培训前后效果。结果三所大学培训前差异无统计学意义（χ^2＝0.081～3.135，P 值均＞0.05）。同一所大学湖南中医药大学两个年级学生，培训前学生对各部分考核存在差异（P＝0.000），基本概念和微生物危害评估掌握较好（P＝0.000，0.000）；培训后学生对各部分考核也存在差异（P＝0.000），基本概念和生物安全操作技术规范掌握较好（P＝0.000，0.002）。同一所大学湖南中医药大学检验专业2008级和2009级的学生培训前后，发现前者除实验室个人防护装备使用外其他均无差异（χ^2＝0.096～2.408，P值均＞0.05）；而后者除基本概念外其他均存在差异（χ^2＝4.821～12.27，P值均＜0.05）。结论生物安全强化培训对动手操作技能有良好的作用，要根据学生的具体情况有的放矢培训，使学生真正受益，保证学生的实验室安全。

语种： 中文

作者： Yin, Tie-qiu*;Xuan Ou-yang（欧阳旋）;Jiao, Fang-yan;Huang, Lu-ping;Tang, Xu-dong;...

期刊： Oncotarget,2016年7(24):37121-37131 ISSN：1949-2553

通讯作者： Yin, Tie-qiu

作者机构： [Xuan Ou-yang; Ren, Bi-qiong; Tang, Xu-dong; Yin, Tie-qiu; Jiao, Fang-yan; Huang, Lu-ping] Hunan Univ Chinese Med, Peoples Hosp Hunan Prov 2, Clin Med Coll, Changsha 410007, Hunan, Peoples R China.

通讯机构： [Yin, Tie-qiu] H;Hunan Univ Chinese Med, Peoples Hosp Hunan Prov 2, Clin Med Coll, Changsha 410007, Hunan, Peoples R China.

关键词： PA-MSHA;PTEN;cervical cancer;p-AKT;p-GSK3beta

摘要： We investigated the effects of Pseudomonas aeruginosa mannose-sensitive hemagglutinin (PA-MSHA) on the proliferation and invasion of human cervical cancer cell lines, as well as the molecular pathways underlying these effects. MTT cell proliferation assays revealed a time- and concentration-dependent cytotoxic effect of PA-MSHA on HeLa cells but not H8 cells. Flow cytometry with propidium iodide and annexin-V-fluorescein isothiocyanate labeling (FITC) indicated that various concentrations of PA-MSHA could induce apoptosis and G2-M cell cycle arrest in HeLa cells. PA-MSHA also impaired the migration and invasion abilities of HeLa cells in Wound healing and Transwell invasion assays. Western blot results demonstrated that PA-MSHA reduced the expression of p-AKT, p-GSK3beta, BCL-2, Vimentin and beta-catenin, but increased the levels of PTEN, BAD, BAX and E-cadherin in HeLa cells. Importantly, PTEN siRNA induced the activity of p-AKT, while PA-MSHA partly inhibited this induction, indicating that PA-MSHA may reduce the cell proliferation and invasion potential by activating PTEN and thus inhibiting the AKT pathway in vitro. These data suggest the potential application of PA-MSHA to the treatment of human cervical cancer.

语种： 英文

作者： 徐国锋;徐飞;任碧琼（任碧琼）;朱浩稳;邹国英;...

期刊： 临床检验杂志,2015年33(9):657-658 ISSN：1001-764X

作者机构： [任碧琼; 徐国锋; 徐飞; 朱浩稳; 邹国英] 湖南省第二人民医院检验科;[李勇] 湖南中医药大学临床医学院

关键词： 全自动分析;温育

摘要： 目的探讨不同温育模式对ADDCARE全自动酶免分析仪上部分检测项目检测结果的影响。方法在全自动酶免分析仪上检测HBsAg和抗HBs,采用仪器自动加盖、不加盖温育以及人工温箱温育3种模式,比较结果间差异。结果3种不同温育模式之间HBsAg S/CO值差异均有统计学意义(P<0.01);仪器加盖与不加盖温育模式抗HBs结果差异无统计学意义(P=1.000),但两种仪器温育模式与人工温箱温育模式相比,结果差异均有统计学意义(P<0.01)。加盖与不加盖对不同浓度抗HBs检测结果无影响。对高浓度阳性和阴性HBsAg标本检测结果无影响,但对cut off值附近临界浓度HBsAg标本影响较大,不加盖温育模式出现假阳性结果。结论全自动酶免分析系统温育是关键环节,检测时应采用加盖温育模式。

语种： 中文

作者： 任碧琼（任碧琼）

期刊： 国际检验医学杂志,2015年(21):3212-3213 ISSN：1673-4130

作者机构： 湖南省第二人民医院检验科;湖南中医药大学临床医学院检验教研室

关键词： 专业岗位;检验医学;检验医师;医学高等教育;医学检验技术;专科教育;执业医师资格;人才培养;医学专业;医学本科

摘要： 国内检验医学在经历了一个飞速发展时期后，硬件设施与发达国家没有什么差距，但在内涵建设上仍存在相当大的提升空间，尤其是人才培养及专业岗位设置方面，很有必要借鉴成功经验。国内检验医学高等教育的历史并不长，20世纪80年代初期才正式建立，先是3年制专科教育，随后设置了旨在培养检验医师的5年制本科教育。然而，2012年国家教育部将高等院校的检验医学专业本科教育改为4年制，命名医学检验技术专业，授予理学学士学位，将检验医学专业的高等教育定位于培养检验技术人员。然而，国内检验医师的培养途径尚未完全明确。虽然随后颁布的住院医师规范化培训制度及检验人员报考执业医师的相关规定中有所体现，但实施起来存在一些问题，本文对此进行了分析和探讨。

语种： 中文

作者： 李勇;徐飞;周卫民;任碧琼（任碧琼）

期刊： 检验医学与临床,2015年(22):3385-3386 ISSN：1672-9455

作者机构： 湖南省中医药大学临床医学院/湖南省第二人民医院检验科,长沙,410007

关键词： 肺炎支原体肺炎;微量元素;C-反应蛋白;儿童

摘要： 目的：探究肺炎支原体肺炎（MPP）儿童微量元素（锌、铜、钙、镁、铁）及C‐反应蛋白（CRP）水平检测的临床意义。方法54例M PP患儿作为观察组，40例健康儿童体检者作为对照组。原子吸收光谱法检测全血铜和锌，比色法检测血清钙、镁、铁离子，免疫透射比浊法检测CRP。结果观察组全血锌、血清铁离子水平明显低于对照组，差异有统计学意义（P＜0．01）；铜、钙、镁水平在两组间差异无统计学意义（均 P＞0．05）；观察组CRP水平明显高于对照组，差异有统计学意义（P＜0．01）。结论 MPP患儿CRP水平明显上升，这提示有炎症存在，同时M PP患儿锌、铁水平明显降低，应及时补充铁、锌来辅助治疗小儿M PP。

语种： 中文

作者： 朱浩稳;徐飞;徐国锋;李勇;邹国英;...

期刊： 检验医学与临床,2015年(24):3629-3630 ISSN：1672-9455

作者机构： 湖南省第二人民医院/湖南中医药大学临床医学院,长沙,410007;[任碧琼; 徐飞; 朱浩稳; 李勇; 邹国英; 徐国锋] 湖南省第二人民医院

关键词： 酶联免疫吸附试验;抗体;人免疫缺陷病毒;阳性预测值

摘要： 目的探讨不同厂家酶联免疫吸附试验（ELISA）试剂检测人免疫缺陷病毒抗体（抗-HIV）结果的差异性。方法同时用两种不同厂家生产的第3代ELISA抗-HIV初筛试剂检测日常标本,初筛阳性结果的标本再用原来的试剂双孔复检排除假阳性,复检阳性的标本送确认实验室做免疫印迹试验（WB试验）确认。结果用A、B两种试剂共检测30批次2 166例标本,经复检A、B两种试剂仍为阳性的标本数分别为9例和19例,其中A、B两种试剂共同阳性反应的标本6例;WB试验确认2例阳性,为A、B两种试剂同时阳性。单独A试剂的阳性预测值为22.22%（2/9）,单独B试剂的阳性预测值为10.53%（2/19）,两种试剂双阳性标本的阳性预测值为33.33%（2/6）。结论不同厂家ELISA试剂检测抗-HIV结果的阳性预测值有较大差异,同时采用两种试剂检测临床标本能提高阳性预测值。

语种： 中文

作者： 任碧琼（任碧琼）

期刊： 中华检验医学杂志,2014年37(12):989-990 ISSN：1009-9158

作者机构： 湖南省第二人民医院检验科;湖南中医药大学临床医学院检验教研室

关键词： 检验医学教育;岗位设置;意大利;检验科人员;大学医院;临床医学;疾病诊治;实验室检验

摘要： 21世纪我国检验医学迅速实现技术的自动化、试剂的商品化、方法的标准化[1]，人员素质也大幅度提高。在近20多年的发展历程中，检验界从业者们进行了各种尝试和探索，尤其在人才培养方面，政府多次对检验人员的教育模式进行改革，从20世纪80年代初期检验高等教育的建立，到随后5年制检验本科的设置，再到2012年始检验本科4年制改制，在某种程度上促进了我国检验医学的发展，使得检验医学教育逐步形成规模，步入正轨。但从检验学科的发展前景来看，尚存在一些待完善的地方。当今我国的检验科人员组成仍限于检验技师，医院没有设置检验医师岗位，检验科只能被归属到辅助科室以及旁系列。笔者2014年在意大利锡耶纳大学医院研修。意大利医院实验室检验医生岗位的明确设置使检验医学被提升到与临床医学等同的高度，整个学科被带动起来，已逐步地从辅助临床、佐证疾病朝着指导临床、引领疾病诊治方向的趋势发展。

语种： 中文

作者： 向万忠;任碧琼（任碧琼）

期刊： 国际检验医学杂志,2013年34(10):1323-1324 ISSN：1673-4130

作者机构： 湖南省脑科医院,湖南长沙410007;湖南中医药大学临床医学院,湖南长沙410208

关键词： 假单胞菌;铜绿;药物耐受性;抗菌药

摘要： 目的监测铜绿假单胞菌的耐药性变迁及探讨耐药机制,为临床提供经验用药参考依据。方法使用MicroscanWalkaway40对铜绿假单胞菌菌株进行鉴定和药物敏感试验。结果从2010～2012年,铜绿假单胞菌对头孢曲松耐药率始终最高,依次为62.8%、64.2%、50.9%。头孢噻肟次之,依次为56.4%、55.7%、44.0%。头孢他啶耐药率依次为33.0%、35.8%、17.1%,亚胺培南的耐药率依次为20.2%、27.4%、19.4%。其余抗菌药物耐药率均低于50.0%,且逐年降低。合计耐药率阿米卡星最低21.8%、头孢曲松最高为57.9%。结论铜绿假单胞菌对常见抗菌药物的耐药率总体呈下降趋势。关注耐药机制有助于临床选用合适抗菌药物提高抗感染疗效。加强耐药性监测有助于减缓细菌耐药性增加趋势。

语种： 中文

作者： 尹铁球;欧阳旋（欧阳旋）;焦芳艳;黄露萍;晏坤;...

期刊： 实用预防医学,2013年20(9):1040-1044 ISSN：1006-3110

作者机构： 湖南中医药大学临床医学院＆湖南省第二人民医院 湖南 长沙 410007

关键词： 宫颈癌;铜绿假单胞菌制剂

摘要： 目的 研究铜绿假单胞菌制剂(pseudomonas aeruginosa with mannose sensitive hemagglutination pili,PAMSHA)由甘露糖依赖方式影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,为治疗宫颈癌的新的生物制剂提供依据. 方法 采用单独处理PA-MSHA或甘露糖与PA-MSHA联合处理宫颈癌Hela细胞,随后采用MTT法检测细胞增殖,流式检测细胞周期,荧光定量PCR和western blot检测凋亡抑制蛋白Bcl-2和促凋亡蛋白Bad、Bax的表达变化. 结果 PA-MSHA单独处理宫颈癌Hela后细胞增殖减慢,细胞周期阻滞在G0/G1期,同时荧光定量PCR和western blot检测显示促凋亡蛋白Bad、Bax的表达增加而凋亡抑制蛋白Bcl-2表达减少.而甘露糖与PAMSHA共培育组与空白组对比在细胞增殖、细胞周期及凋亡蛋白表达上差异无统计学意义. 结论 PA-MSHA可以影响Bad、Bax及Bcl-2的表达进而影响癌细胞的增殖和细胞周期进程,且这种作用为甘露糖敏感型,该结果为其成为治疗宫颈癌的新的生物制剂提供了可靠依据.

语种： 中文